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The map label for this gene is rlmI [H]

Identifier: 54297147

GI number: 54297147

Start: 1331712

End: 1332884

Strand: Direct

Name: rlmI [H]

Synonym: lpp1192

Alternate gene names: 54297147

Gene position: 1331712-1332884 (Clockwise)

Preceding gene: 54297143

Following gene: 54297148

Centisome position: 38.01

GC content: 39.81

Gene sequence:

>1173_bases
ATGAAAGCGAAAGTTATTTTAGTGAATGGAAAGCAAAACACTGTATTAAGAGGTCATCCCTGGATTTACCCCAAAGCCAT
AGCTCAATATTCTGGCAAGCTAATTACAGGTGAATTGGTTGAGATAATTGGCTCTGAAGGTGAGAGTTTAGGATTCGGGG
CCTATAATGAGCACTCTTTATATAGAGTAAGGGTTCTTGCTCTCTCCAATGAACAGATAAATAGTTTCGATTACAAGTCT
TTAATTACTCATAGAATTAAGCAAGCCAGTCTGGTGAGACGATGTCTTGATTTACCAAATGAACAGACTAATGCTTATCG
ATTGTTCAACAGCGAAGCGGATGGGCTCTCTGGTTTAACGATTGATTGCTTTAATGACTATTGCGTTATAGCAAGTTCTG
CTTATTGGGTAGAATTGAATAAATCGATTATTGTTCAGGTATTGCAAGAGTTATTCCCGCATAGTCATGTCATTTGGCTT
CCTCAAAACAAGCCGTTATCTCAAGATGGCTGGAAAGAAATTCATACAGAAAAAAGTCAGGGTAGCACTCAGGTTTTGGA
AGAAGGTGTTCTGTATCATGTTGAATTTGCGCAAACCCAAAAAACTGGATTGTTTCTTGACCAAAGAGAAAATCACAAAA
GGATAGCAAAACTATCCAGGGGAAAAAGAGTCCTGGATCTTTATACTTTTACGGGAGGGTTTGCTTTGCACGCAGCGAAA
GCGGGCGCTCTTCAAGTTACGGCTGTAGATAGCTCGGCGCAGGCAATAGAGCAGGCTAAAAATAATGCTGTTTTGAATCA
CTTAAATACCATTGAATTTATCAAGGCAGATGCCAGAGAATATCTGAAAATGGCTGGCGAATATGATGTGGTCATTCTTG
ACCCGCCAAAACTGGTTCCTTCACAAAAACATTTACAGCAAGCTAAAAATTATTACCGATTTTTACATAGAGAAGCATTT
AAATATATGAAACCTGGATCTTTATTGATGACTTGCAACTGTTCTTCAGCACTTTCATCCCAGGATTTTTGTTCCCTGGT
AAGCGCTCAGGCTGTTGCGATGGGAAAGCAGGCGCGTATTTTAGGCGTATACGGACCAGCAAGCTGCCATCCTACTTTGG
CAGCCTTTCCAGAGGGAAATTATTTAACAGCTGTTTTATTAGCATTGGTATAA

Upstream 100 bases:

>100_bases
AGGTATTTTGTAAATTGCCGCATATTTGTCCTTCTTTCCATTGCAAGTATAATTCTTGATAGTGTAGATTAACTGACTTT
TTGGTTTTGGGAAATAGAGA

Downstream 100 bases:

>100_bases
AAGGAACCTTGATTTTTAAATCAAGGATGGTATTAGGTTGATTTTTTTGTATAGAATTTGATTAGTTTGTCCTTAATATG
TATAATTGGCGCATGTCGCA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKAKVILVNGKQNTVLRGHPWIYPKAIAQYSGKLITGELVEIIGSEGESLGFGAYNEHSLYRVRVLALSNEQINSFDYKS
LITHRIKQASLVRRCLDLPNEQTNAYRLFNSEADGLSGLTIDCFNDYCVIASSAYWVELNKSIIVQVLQELFPHSHVIWL
PQNKPLSQDGWKEIHTEKSQGSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKLSRGKRVLDLYTFTGGFALHAAK
AGALQVTAVDSSAQAIEQAKNNAVLNHLNTIEFIKADAREYLKMAGEYDVVILDPPKLVPSQKHLQQAKNYYRFLHREAF
KYMKPGSLLMTCNCSSALSSQDFCSLVSAQAVAMGKQARILGVYGPASCHPTLAAFPEGNYLTAVLLALV

Sequences:

>Translated_390_residues
MKAKVILVNGKQNTVLRGHPWIYPKAIAQYSGKLITGELVEIIGSEGESLGFGAYNEHSLYRVRVLALSNEQINSFDYKS
LITHRIKQASLVRRCLDLPNEQTNAYRLFNSEADGLSGLTIDCFNDYCVIASSAYWVELNKSIIVQVLQELFPHSHVIWL
PQNKPLSQDGWKEIHTEKSQGSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKLSRGKRVLDLYTFTGGFALHAAK
AGALQVTAVDSSAQAIEQAKNNAVLNHLNTIEFIKADAREYLKMAGEYDVVILDPPKLVPSQKHLQQAKNYYRFLHREAF
KYMKPGSLLMTCNCSSALSSQDFCSLVSAQAVAMGKQARILGVYGPASCHPTLAAFPEGNYLTAVLLALV
>Mature_390_residues
MKAKVILVNGKQNTVLRGHPWIYPKAIAQYSGKLITGELVEIIGSEGESLGFGAYNEHSLYRVRVLALSNEQINSFDYKS
LITHRIKQASLVRRCLDLPNEQTNAYRLFNSEADGLSGLTIDCFNDYCVIASSAYWVELNKSIIVQVLQELFPHSHVIWL
PQNKPLSQDGWKEIHTEKSQGSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKLSRGKRVLDLYTFTGGFALHAAK
AGALQVTAVDSSAQAIEQAKNNAVLNHLNTIEFIKADAREYLKMAGEYDVVILDPPKLVPSQKHLQQAKNYYRFLHREAF
KYMKPGSLLMTCNCSSALSSQDFCSLVSAQAVAMGKQARILGVYGPASCHPTLAAFPEGNYLTAVLLALV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=392, Percent_Identity=29.8469387755102, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1787181, Length=156, Percent_Identity=31.4102564102564, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43420; Mature: 43420

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKVILVNGKQNTVLRGHPWIYPKAIAQYSGKLITGELVEIIGSEGESLGFGAYNEHSL
CCEEEEEEECCCCCEEECCCCCCHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCCCCCCE
YRVRVLALSNEQINSFDYKSLITHRIKQASLVRRCLDLPNEQTNAYRLFNSEADGLSGLT
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCE
IDCFNDYCVIASSAYWVELNKSIIVQVLQELFPHSHVIWLPQNKPLSQDGWKEIHTEKSQ
EEECCCEEEEECCEEEEEECHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCC
GSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKLSRGKRVLDLYTFTGGFALHAAK
CHHHHHHCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHCCCEEEEEEEECCCEEEEECC
AGALQVTAVDSSAQAIEQAKNNAVLNHLNTIEFIKADAREYLKMAGEYDVVILDPPKLVP
CCEEEEEEECCHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
SQKHLQQAKNYYRFLHREAFKYMKPGSLLMTCNCSSALSSQDFCSLVSAQAVAMGKQARI
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHCCCCEEE
LGVYGPASCHPTLAAFPEGNYLTAVLLALV
EEEECCCCCCCCEEECCCCCHHHHHHHHHC
>Mature Secondary Structure
MKAKVILVNGKQNTVLRGHPWIYPKAIAQYSGKLITGELVEIIGSEGESLGFGAYNEHSL
CCEEEEEEECCCCCEEECCCCCCHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCCCCCCE
YRVRVLALSNEQINSFDYKSLITHRIKQASLVRRCLDLPNEQTNAYRLFNSEADGLSGLT
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCE
IDCFNDYCVIASSAYWVELNKSIIVQVLQELFPHSHVIWLPQNKPLSQDGWKEIHTEKSQ
EEECCCEEEEECCEEEEEECHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCC
GSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKLSRGKRVLDLYTFTGGFALHAAK
CHHHHHHCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHCCCEEEEEEEECCCEEEEECC
AGALQVTAVDSSAQAIEQAKNNAVLNHLNTIEFIKADAREYLKMAGEYDVVILDPPKLVP
CCEEEEEEECCHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
SQKHLQQAKNYYRFLHREAFKYMKPGSLLMTCNCSSALSSQDFCSLVSAQAVAMGKQARI
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHCCCCEEE
LGVYGPASCHPTLAAFPEGNYLTAVLLALV
EEEECCCCCCCCEEECCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA