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The map label for this gene is citH [H]

Identifier: 54297120

GI number: 54297120

Start: 1295575

End: 1296861

Strand: Direct

Name: citH [H]

Synonym: lpp1165

Alternate gene names: 54297120

Gene position: 1295575-1296861 (Clockwise)

Preceding gene: 54297118

Following gene: 54297121

Centisome position: 36.98

GC content: 38.31

Gene sequence:

>1287_bases
ATGAATACATCAAATACCTCCTTTAAAACATTGGGAGCAATATGTATTGGTAATGCTATGGAATGGTATGATTTCATGAT
TTATAACTTCATGCTGCTTTTTATTGCCCAAGCTTTTTTTCCCGATCATAGTCCTGTGACTTCCCTCTTGGCTACAATGG
CAAGCTCCGGAGTGGCTCTGGTAGTCCGGCCTCTTGGTGGTTTTATTTTAGCAGTTTGGGCAGATAAGTATGGGCATCAT
AAAGCATTAATGTTCGTTTTTTATTTGATGGCTGTTGCAACTATGCTTATTGCTTTTACACCATCCTACTCCACAATAGG
TCTGGCTGCTACTTGTGTCATTGTATTTGCGAGATTGTTGCAAGGGCTTGCTACTGGTGGCGAGTTTGGCATATCCAGCG
CTCTATTAGTTAGCTTATCGCCAAGCGATAAAAAGGGGTTTTACACTTCCTTACAAATGGTAGGCCAGCTTTTGGCAATA
TTAATGGGATCTTCAGTGTGCCTTATTTTAACTCTCTATTGTTCCAATGAAACCCTATATCAATTTGCCTGGAGAATACC
TTTTGCTTTAGGACTATTGATTATACCTTTAGGATGGATTTTAAGACGTCGATTGCAACGGGAATTTACTTGGAAGAAAG
AGCAACAGGACACAGGAAAGCTACTTATCATGGTAAAGCAACAAAAACGTTCGCTGTTGATAGCTTTTAGTCTTGTAGCC
GGGTGTACAGGCTCGATTTATACCTTATTCAGCTATATGCCTACCTATTGTAAATTGTACTTGAATTTATCGCTAACAGA
GGCGTACTTAGGCACTGCATCTGGAATATTAGTCAGTATTCTTACTATTCCCTTTTTTGGAGCATTGTCGGATAGAATAG
GCAAAAAAATAGTTATGTTATGTGCTTTGGCTTGTTATTTTGGATTAATTTATCCTTTACTTTTCTTGCTCAACCAATAC
CCTAGTGTAACTATTCTCATTTTGGTTGAAAACATTTTAGGTTTGTGTATCGGCGCTTATTTTGGAGTGTTAACCATTAC
AGTTAGCTCCTTATTTCCACCATCAGTACGTTCTACTTGTTTAGCCGTCAGCTATAATTCAGCTGTCCTGTTGTTTGGTG
GTTTTGCTCCATTTATTATAACAGCGTTGATTGAATACACTAAAAACCCTATGGTTTTTACTTATTATCAAATGACGACA
GTCTTTATCAGTTTGCTAGCAGTCCTTTCTTATCAGGAGGAAAGGAAGCTATCTGAGAATCAGCCAGAACATCTCGTTGC
AATTTAA

Upstream 100 bases:

>100_bases
AGTAAAATTAATATTGATATTAGTTTCTTATAAAGCATGGTAATTTTTTTTATATTCTACTAGTATATGCTTTGCTCTAA
ATTTACCCTTGTAAGGAAAC

Downstream 100 bases:

>100_bases
AAGGTAGGAATATGACAACTTTAGATTGGTTAAAACAACTCATTTCTTTTGATACTACTTCGCGGAACTCTAATTTATCA
TTGATTGAATATTTGGCGAA

Product: hypothetical protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPDHSPVTSLLATMASSGVALVVRPLGGFILAVWADKYGHH
KALMFVFYLMAVATMLIAFTPSYSTIGLAATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
LMGSSVCLILTLYCSNETLYQFAWRIPFALGLLIIPLGWILRRRLQREFTWKKEQQDTGKLLIMVKQQKRSLLIAFSLVA
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPFFGALSDRIGKKIVMLCALACYFGLIYPLLFLLNQY
PSVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTT
VFISLLAVLSYQEERKLSENQPEHLVAI

Sequences:

>Translated_428_residues
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPDHSPVTSLLATMASSGVALVVRPLGGFILAVWADKYGHH
KALMFVFYLMAVATMLIAFTPSYSTIGLAATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
LMGSSVCLILTLYCSNETLYQFAWRIPFALGLLIIPLGWILRRRLQREFTWKKEQQDTGKLLIMVKQQKRSLLIAFSLVA
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPFFGALSDRIGKKIVMLCALACYFGLIYPLLFLLNQY
PSVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTT
VFISLLAVLSYQEERKLSENQPEHLVAI
>Mature_428_residues
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPDHSPVTSLLATMASSGVALVVRPLGGFILAVWADKYGHH
KALMFVFYLMAVATMLIAFTPSYSTIGLAATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
LMGSSVCLILTLYCSNETLYQFAWRIPFALGLLIIPLGWILRRRLQREFTWKKEQQDTGKLLIMVKQQKRSLLIAFSLVA
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPFFGALSDRIGKKIVMLCALACYFGLIYPLLFLLNQY
PSVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTT
VFISLLAVLSYQEERKLSENQPEHLVAI

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=29.8368298368298, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788942, Length=413, Percent_Identity=29.7820823244552, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788292, Length=422, Percent_Identity=29.6208530805687, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789941, Length=433, Percent_Identity=26.5588914549654, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI87082404, Length=359, Percent_Identity=23.9554317548747, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47281; Mature: 47281

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPDHSPVTSLLATMASSGVAL
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
VVRPLGGFILAVWADKYGHHKALMFVFYLMAVATMLIAFTPSYSTIGLAATCVIVFARLL
EEEHHCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAILMGSSVCLILTLYCSNETLY
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHEEEEHCCCCHHH
QFAWRIPFALGLLIIPLGWILRRRLQREFTWKKEQQDTGKLLIMVKQQKRSLLIAFSLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHH
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPFFGALSDRIGKKIVML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CALACYFGLIYPLLFLLNQYPSVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTC
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHE
LAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTTVFISLLAVLSYQEERKLSEN
EEEEECCEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
QPEHLVAI
CCCCCCCC
>Mature Secondary Structure
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPDHSPVTSLLATMASSGVAL
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
VVRPLGGFILAVWADKYGHHKALMFVFYLMAVATMLIAFTPSYSTIGLAATCVIVFARLL
EEEHHCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAILMGSSVCLILTLYCSNETLY
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHEEEEHCCCCHHH
QFAWRIPFALGLLIIPLGWILRRRLQREFTWKKEQQDTGKLLIMVKQQKRSLLIAFSLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHH
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPFFGALSDRIGKKIVML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CALACYFGLIYPLLFLLNQYPSVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTC
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHE
LAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTTVFISLLAVLSYQEERKLSEN
EEEEECCEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
QPEHLVAI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]