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The map label for this gene is artJ [H]

Identifier: 54297081

GI number: 54297081

Start: 1249226

End: 1249921

Strand: Direct

Name: artJ [H]

Synonym: lpp1126

Alternate gene names: 54297081

Gene position: 1249226-1249921 (Clockwise)

Preceding gene: 54297078

Following gene: 54297082

Centisome position: 35.66

GC content: 34.77

Gene sequence:

>696_bases
ATGATTCTAAAGCCGGGAAAATTATTGGTGGCTACTGCTTATCCAGATCCACCGTTTGATATTGAGCCAGGTGATGGTTT
TGATGGTGATTTAATAAAAGAAATTTGTCGAATTTTAAATTTGCAGCTAGTCCAGATCAAATATGAGGGAGAAAATTTTA
ATGATATTTTTTTAGGCTTGGCTAATAAAGAATACGATGTTGTCATTTCAGGAACGACTATTACACCAAACCGATCAAAA
CAAGTACTTTTTTCGAATCCCTATTTAGAGTTTAATCAGGGTATCGCGGTTAATACTGAATTAAAGCCAAATGTTTCTTC
TTTTGAAGATTTGAAAGGTTTGGTGGCTGGTATACAGAAAGGCAACACCTCAGATGAAGTTGCTCAGGATTTATTACGTA
AGAACATACTCAGTGATATTAAATATTATGCATACAATGAAATAGAATTAGCCATACAGGATCTGTCTGCCGGTAAAATT
GGTTTGGTCATTAAATTATATCCTGTTATTTCCTGTTTAATTCGAAACTATACAAACCTGGCTGTTCCTTTGCAGGCTGA
AACACATGAAAAACTTCGTATCGCGGTTGCTAAAGATAATTTTAAACTACGGGATAAAGTCAATCTTATTTTGGCCGATC
TTATGCAAAATGGTACTTTAAGTCGTCTGCAACATAAGTGGATAAGTACAACCTAG

Upstream 100 bases:

>100_bases
GTAAATTATTCTTTTAAATCAATGGCATAAAAAATATTTGGCTGATTTTTTTCTGATTAATCTATACTTAGTGAATCGAT
TCCAAACAATAGTTATATCT

Downstream 100 bases:

>100_bases
GGTAAATTAGGCCAATGTCAGTTTCAACTCAAGAAATAACCTTATTTAAGGAATCTTATTTTTCTTGAATCGTTCTGTAA
TTATTTTTCAAATCCTCTTG

Product: hypothetical protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSK
QVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQKGNTSDEVAQDLLRKNILSDIKYYAYNEIELAIQDLSAGKI
GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLRIAVAKDNFKLRDKVNLILADLMQNGTLSRLQHKWISTT

Sequences:

>Translated_231_residues
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSK
QVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQKGNTSDEVAQDLLRKNILSDIKYYAYNEIELAIQDLSAGKI
GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLRIAVAKDNFKLRDKVNLILADLMQNGTLSRLQHKWISTT
>Mature_231_residues
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSK
QVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQKGNTSDEVAQDLLRKNILSDIKYYAYNEIELAIQDLSAGKI
GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLRIAVAKDNFKLRDKVNLILADLMQNGTLSRLQHKWISTT

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=221, Percent_Identity=31.2217194570136, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787088, Length=227, Percent_Identity=28.1938325991189, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1787031, Length=232, Percent_Identity=31.0344827586207, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1788228, Length=231, Percent_Identity=26.8398268398268, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 25840; Mature: 25840

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGL
CCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEEECCCCCCEEEEEE
ANKEYDVVISGTTITPNRSKQVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQK
CCCCEEEEEECCEECCCCCCEEEEECCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHC
GNTSDEVAQDLLRKNILSDIKYYAYNEIELAIQDLSAGKIGLVIKLYPVISCLIRNYTNL
CCCHHHHHHHHHHHHHHHHHHHHEECEEEEEEEECCCCCEEEEEEHHHHHHHHHHCCCCE
AVPLQAETHEKLRIAVAKDNFKLRDKVNLILADLMQNGTLSRLQHKWISTT
EEEEECCCCCEEEEEEECCCEEEHHHHHHHHHHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGL
CCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEEECCCCCCEEEEEE
ANKEYDVVISGTTITPNRSKQVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQK
CCCCEEEEEECCEECCCCCCEEEEECCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHC
GNTSDEVAQDLLRKNILSDIKYYAYNEIELAIQDLSAGKIGLVIKLYPVISCLIRNYTNL
CCCHHHHHHHHHHHHHHHHHHHHEECEEEEEEEECCCCCEEEEEEHHHHHHHHHHCCCCE
AVPLQAETHEKLRIAVAKDNFKLRDKVNLILADLMQNGTLSRLQHKWISTT
EEEEECCCCCEEEEEEECCCEEEHHHHHHHHHHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]