Click here to switch to the map view.

The map label for this gene is pilQ [H]

Identifier: 54296952

GI number: 54296952

Start: 1098147

End: 1100243

Strand: Direct

Name: pilQ [H]

Synonym: lpp0993

Alternate gene names: 54296952

Gene position: 1098147-1100243 (Clockwise)

Preceding gene: 54296951

Following gene: 54296953

Centisome position: 31.34

GC content: 38.72

Gene sequence:

>2097_bases
GTGAGAAAAATTATCGCGATTTTGATATTAATTAGTGCCAGTTTGAGCCTGGCATATTCTAAAGATAATTCGCTCATTTC
AGTACAAGTATTGCCATTGCCCGAAAACAGAGTACGTATTGATTTTCAATTTTCCAAGCCCTTAAAGCAGTTGCCCGCAA
GCTTTATTACTCAAAAGCCGCCTCGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCTTCGGATCAGAAAACT
AAAACTATTCAATTAGGCTCATTACACAGTTATAACATTGTTGCTGTTGGTGATAGAGTAAGAGCCATATTAGAACTGGA
TCGTTCAGTGTCCTATTCTGGCAGTACGGCTGGTTCCGTTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTATTTG
AAAATAGCAAGGAGGTATTCATAACGAACCAGGTTGTGAACGCCAAGCATGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAGGGAGGTAGAGTTTTAATTGATGTTTCTGATACGGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAATTTCTTGAATACCAAGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACGATGCAACAGGAAGGCAAAAAGGTTCGCATGACCATCCTCAGCAAAGGGGATTATGGGCATTTTGTTTAT
CAGGTCAATAAACAATTTATGGTCGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTGAAGAAACAGGT
ATTTACCGGTAAACGGATTTCTTTGAATTTCCAGAATATTAGTATTCGAGCTGTGCTTCAACTTCTGGCAGATTTTACCG
GAATCAATATGGTTGTCAGTGACAAAGTGCAAGGAGATATTACGCTTAGATTAAATGATATCCCCTGGGATCAGGCTCTT
GATATTATTTTAACCACACAAGGGCTAGATAAGCGCAGAAAGGGCAACGTCATGTTGATAGATACCAAAGCCTCCATGGA
TAAAATGGAGGAAGAACAACTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATCCGCTCTGATTTAATACAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAACTCCCTATTATCAGACAGAGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCTGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTAACTAAAGATTTTAGCCAGGATATTGGAATAC
GCTGGGGGGTATCAAGACCAACTCACTTAAGTGGTACACTGGAAGGTGCTAATGAGTTAGCGAGAGGCACAGCACCAGCC
AATGTTGTCCCCTTGGAAAGAAGGTTAAATCTTGATTTGGCAGCTGCTCCTTTAACTGGAGCTACACCGGCCTCGGTAGG
TATTGCGCTAGCTAAACTTAATGATAACATTCTGCTTGATCTGGAGTTGTCTGCTTTGGAAAGCGAAGGGCGTGCTGAAT
TAATATCCAGTCCTCGCCTGATTACTACGAATCAACAGGCAGCAGTCATAGAGTCTGGTGAGGAGATTCCCTATCAGGAG
GCAACATCAAGTGGAGCGACTGCTGTTGCATTTAAAAAAGCAGTATTGAGTTTGAAAGTTACGCCGCAAATTACGCCTGA
TGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCCCCACAAACATTCCTTGGTGTTCCAGCGATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGAGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAAGAGTTACTTATTTTTATTACACCAAGAATAATAACGAATGCCTTATCGATTACAACGATAGAGGGACGTGAGA
AAGATGTTTATAAATAA

Upstream 100 bases:

>100_bases
AAATGATCTCATTAAATTGGAAGAAACTGTACAGAAATCAGGAACGTGGGAAAAACAGTCCACCACAATTAATTTGGATA
CTGGCAAGCAGGAGTGAGGC

Downstream 100 bases:

>100_bases
TTAATATTGGACTAATTTTATCTCATTAATAAAGATAAAAAACATTAATTTAATAGAAGTGCCTCAACACGGTGAACATG
TTATAATTAAAATATAGCCC

Product: type IV pilus (Tfp) assembly protein PilQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MRKIIAILILISASLSLAYSKDNSLISVQVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Sequences:

>Translated_698_residues
MRKIIAILILISASLSLAYSKDNSLISVQVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK
>Mature_698_residues
MRKIIAILILISASLSLAYSKDNSLISVQVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Specific function: Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a "porthole" allowing passage of the fimbrae through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=420, Percent_Identity=33.3333333333333, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI87082242, Length=364, Percent_Identity=25.8241758241758, Blast_Score=100, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 77457; Mature: 77457

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIIAILILISASLSLAYSKDNSLISVQVLPLPENRVRIDFQFSKPLKQLPASFITQKP
CHHHHHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEEEEEECCHHHHCCHHHHCCCC
PRLVLDFISTDMQIPSDQKTKTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSV
HHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCCEE
YSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGIEKQGGRVLIDVSDTGIPIDV
EEEEEECCCCCCCCCCCEEEEEEEHHCCHHHHEECCCCCCCCCCCEEEEEECCCCCEEEH
TQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEE
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVS
EECCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCEEHHHHHHHHHCCCCCEEEEE
DKVQGDITLRLNDIPWDQALDIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTI
CCCCCEEEEEEECCCHHHCEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
EKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKISVDARTNTIWIQDTGTKIEEV
HHHHHHHHHHEEEEECCCCCEEEEEECCCCCHHCCCCCEEEEECCCEEEEEECCCHHHHH
RELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHCCCCCC
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRL
CEEEHHHHCCCEEEECCCCCCCCHHHCEEEEEECCCEEEEEEEHHCCCCCCHHHHCCCEE
ITTNQQAAVIESGEEIPYQEATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDT
EECCCCEEEEECCCCCCCHHCCCCCCCHHEEEEEEEEEEECCEECCCCCEEEEEEECCCC
PSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKNKVINRIPFFGELPVVGILFS
CCCCEEECCCHHEECCCEEEEEEECCCEEEEEEEEECCHHHHHHHCCCCCCCCEEEEEEC
NRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK
CCEEEEECCEEEEEECHHHHHCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MRKIIAILILISASLSLAYSKDNSLISVQVLPLPENRVRIDFQFSKPLKQLPASFITQKP
CHHHHHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEEEEEECCHHHHCCHHHHCCCC
PRLVLDFISTDMQIPSDQKTKTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSV
HHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCCEE
YSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGIEKQGGRVLIDVSDTGIPIDV
EEEEEECCCCCCCCCCCEEEEEEEHHCCHHHHEECCCCCCCCCCCEEEEEECCCCCEEEH
TQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEE
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVS
EECCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCEEHHHHHHHHHCCCCCEEEEE
DKVQGDITLRLNDIPWDQALDIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTI
CCCCCEEEEEEECCCHHHCEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
EKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKISVDARTNTIWIQDTGTKIEEV
HHHHHHHHHHEEEEECCCCCEEEEEECCCCCHHCCCCCEEEEECCCEEEEEECCCHHHHH
RELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHCCCCCC
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRL
CEEEHHHHCCCEEEECCCCCCCCHHHCEEEEEECCCEEEEEEEHHCCCCCCHHHHCCCEE
ITTNQQAAVIESGEEIPYQEATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDT
EECCCCEEEEECCCCCCCHHCCCCCCCHHEEEEEEEEEEECCEECCCCCEEEEEEECCCC
PSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKNKVINRIPFFGELPVVGILFS
CCCCEEECCCHHEECCCEEEEEEECCCEEEEEEEEECCHHHHHHHCCCCCCCCEEEEEEC
NRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK
CCEEEEECCEEEEEECHHHHHCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7901733; 10984043 [H]