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The map label for this gene is ald [H]

Identifier: 54296945

GI number: 54296945

Start: 1090182

End: 1091303

Strand: Direct

Name: ald [H]

Synonym: lpp0986

Alternate gene names: 54296945

Gene position: 1090182-1091303 (Clockwise)

Preceding gene: 54296944

Following gene: 54296948

Centisome position: 31.12

GC content: 44.21

Gene sequence:

>1122_bases
ATGTTAGTAGGGGTACCAAAAGAAATCAAATCGCACGAAAACAGAGTAGGACTTGTTCCCTCGAGTGTTAGAGAAATTAT
TAGGGCAGGAAGTGAAGTATTGGTAGAGAAGGGAGCTGGGCTTGGTATTGGCATATCGGATGATGATTACAGAAATGCCG
GGGCTGAAGTAGTTGATACTGCTGATGACGTATTTGCCAATGCTGAATTAATAGTCAAAGTAAAAGAGCCTCAGCCTATT
GAATGTAAACGGTTACGTGAAGGGCAAACATTATTCACTTATTTACATTTGGCTCCTGATCCTCATCAAGCGCGTTTATT
AAAAGAATCCGGAGTTACAGCGATAGCTTATGAAACTGTAACACAGGATGATGGCGGGCTACCTTTACTTACTCCTATGT
CCCAAGTGGCTGGTCGTATGGCAATCCAGGCCGGTGCACATTGCTTGGAGATGGCTCAAGGGGGTAGCGGTATATTACTA
GGTGGTGTACCTGGTGTTGCTGCAGCCAATGTCGTAGTCATTGGCGGGGGCGTTGTAGGAACAAATGCTGTTCGCATGGC
GATGGGGATGGAAGCTCGTGTTATTGTGCTGGATCGTTCACTGCAACGTTTAAATGAATTGGATTTTCAGTTTGGTTCTA
AACTCAATACAGTTTACTCGACTGCAGATTCCATAGAAAAATACGTATCCAGCGCTGATTTAGTGATTGGTGCTGTATTG
GTACCAGGTGCTGCAGCCCCAAAATTAGTGACTCGCTCTATGCTGAGCTCAATGCGTCCTGGTTCTGTTTTAGTCGACGT
TGCTATTGATCAGGGAGGCTGTTTTGAGACAAGTCGTGCGACAACCCATCAAGAACCTACTTACGTGGTCGATGGCGTAG
TGCATTATTGTGTGGCCAATATGCCTGGTGCTGTACCCAGAACATCAACGTTTGCTTTAAATAATGCCACATTACCTTTC
ATTTTAAGTATTGTTTCCAAAGGGGTTAAGCTGGCTTTATTGAGTGACCCGCATTTACTTAATGGTTTAAATGTTCACCA
GGGTAAAATCACTTATGAGGCTGTTGCTCGTGATTTAGGATATGAATACACACCAGCTGCAGCTGCTTTATCAGGACGAT
AA

Upstream 100 bases:

>100_bases
CACCTATTTTTCAAATTGCGACTTATGGGTTAGTAGGTGATCTGTTTGAATTAGTTCCACAATTAATAGAACATTTAAAG
AATCGATAAGAGGTAACAAT

Downstream 100 bases:

>100_bases
TTGTTAGTTAAAACCCGTATTACAAAAATTGAATACGGGTTTTGGGCTTCATCAGAATATAAATCTTCAAATTAAAACAA
ACTTTCTTCCACTGGCGCTA

Product: hypothetical protein

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLVGVPKEIKSHENRVGLVPSSVREIIRAGSEVLVEKGAGLGIGISDDDYRNAGAEVVDTADDVFANAELIVKVKEPQPI
ECKRLREGQTLFTYLHLAPDPHQARLLKESGVTAIAYETVTQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILL
GGVPGVAAANVVVIGGGVVGTNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTVYSTADSIEKYVSSADLVIGAVL
VPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFETSRATTHQEPTYVVDGVVHYCVANMPGAVPRTSTFALNNATLPF
ILSIVSKGVKLALLSDPHLLNGLNVHQGKITYEAVARDLGYEYTPAAAALSGR

Sequences:

>Translated_373_residues
MLVGVPKEIKSHENRVGLVPSSVREIIRAGSEVLVEKGAGLGIGISDDDYRNAGAEVVDTADDVFANAELIVKVKEPQPI
ECKRLREGQTLFTYLHLAPDPHQARLLKESGVTAIAYETVTQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILL
GGVPGVAAANVVVIGGGVVGTNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTVYSTADSIEKYVSSADLVIGAVL
VPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFETSRATTHQEPTYVVDGVVHYCVANMPGAVPRTSTFALNNATLPF
ILSIVSKGVKLALLSDPHLLNGLNVHQGKITYEAVARDLGYEYTPAAAALSGR
>Mature_373_residues
MLVGVPKEIKSHENRVGLVPSSVREIIRAGSEVLVEKGAGLGIGISDDDYRNAGAEVVDTADDVFANAELIVKVKEPQPI
ECKRLREGQTLFTYLHLAPDPHQARLLKESGVTAIAYETVTQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILL
GGVPGVAAANVVVIGGGVVGTNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTVYSTADSIEKYVSSADLVIGAVL
VPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFETSRATTHQEPTYVVDGVVHYCVANMPGAVPRTSTFALNNATLPF
ILSIVSKGVKLALLSDPHLLNGLNVHQGKITYEAVARDLGYEYTPAAAALSGR

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=346, Percent_Identity=30.0578034682081, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI122939153, Length=346, Percent_Identity=30.0578034682081, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1787887, Length=380, Percent_Identity=30.5263157894737, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17550456, Length=346, Percent_Identity=31.2138728323699, Blast_Score=131, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39182; Mature: 39182

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVGVPKEIKSHENRVGLVPSSVREIIRAGSEVLVEKGAGLGIGISDDDYRNAGAEVVDT
CCCCCCHHHHCCCCEECCCHHHHHHHHHCCCCEEEECCCCCEECCCCCCHHCCCCHHHCC
ADDVFANAELIVKVKEPQPIECKRLREGQTLFTYLHLAPDPHQARLLKESGVTAIAYETV
HHHHHCCCEEEEEECCCCCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCEEEEEEEE
TQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILLGGVPGVAAANVVVIGGGVVG
ECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCEEC
TNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTVYSTADSIEKYVSSADLVIGAVL
CHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHEEEHEE
VPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFETSRATTHQEPTYVVDGVVHYCVAN
CCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
MPGAVPRTSTFALNNATLPFILSIVSKGVKLALLSDPHLLNGLNVHQGKITYEAVARDLG
CCCCCCCCCEEEECCCCHHHHHHHHHCCEEEEEECCCCHHCCCCCCCCEEHHHHHHHHCC
YEYTPAAAALSGR
CCCCCHHHHCCCC
>Mature Secondary Structure
MLVGVPKEIKSHENRVGLVPSSVREIIRAGSEVLVEKGAGLGIGISDDDYRNAGAEVVDT
CCCCCCHHHHCCCCEECCCHHHHHHHHHCCCCEEEECCCCCEECCCCCCHHCCCCHHHCC
ADDVFANAELIVKVKEPQPIECKRLREGQTLFTYLHLAPDPHQARLLKESGVTAIAYETV
HHHHHCCCEEEEEECCCCCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCEEEEEEEE
TQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILLGGVPGVAAANVVVIGGGVVG
ECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCEEC
TNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTVYSTADSIEKYVSSADLVIGAVL
CHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHEEEHEE
VPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFETSRATTHQEPTYVVDGVVHYCVAN
CCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
MPGAVPRTSTFALNNATLPFILSIVSKGVKLALLSDPHLLNGLNVHQGKITYEAVARDLG
CCCCCCCCCEEEECCCCHHHHHHHHHCCEEEEEECCCCHHCCCCCCCCEEHHHHHHHHCC
YEYTPAAAALSGR
CCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]