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The map label for this gene is mraW

Identifier: 54296934

GI number: 54296934

Start: 1079352

End: 1080278

Strand: Direct

Name: mraW

Synonym: lpp0975

Alternate gene names: 54296934

Gene position: 1079352-1080278 (Clockwise)

Preceding gene: 54296933

Following gene: 54296935

Centisome position: 30.81

GC content: 40.56

Gene sequence:

>927_bases
ATGGCAAAGCATCAATCTGTTTTATTACATGAATCAATCAAAGGTTTGGCTATAAAAGCTGACGGTATCTATTTTGATGG
AACTTTTGGCCGAGGAGGCCACAGCCGCGAGATTTTAAATCATTTAAGCGATAAAGGAAGATTGTTTGCAATAGATAAGG
ACCTTGATGCGGTGCAATATGCCAAGGATTATTTTGGTTTGGATAAACGATTTCAAATTTTTCACGGTTCATTCGCGCAA
ATCAAGGAATTTGCGTCACAAGCGGGGGTAATTGGGGCCGTTGATGGAATTTTGCTTGATCTGGGAGTGTCTTCACCTCA
GTTGGACAATCCAGAAAGAGGTTTTAGTTTTATGCTGCAAGGCCCTCTCGATATGCGAATGGATTTAACTCAATCAATTA
ATGCTGCGAATTTTGTTAATGAAGCAGAGGTCAACGAGCTGGCTCATGTATTCAGGGCTTATGGTGAGGAACGATTTGCA
GGCAGGATTGCAAAAGCTATTGTTGATGCAAGAAAACTAAAACCAATAACAACCACTTTGGAGTTAGCGGAAATTGTTAA
AGAAGCAAACCCCAAATGGGAAAAACACAAACATCCTGCAACCCGGGTATTTCAAGCGATTCGAATACATGTCAATCAGG
AATTAACCGATTTGAGTAATTGTCTAGAGCAATGTCTGGACGTGTTGGGGCCAGGAGGACGCTTGGCCGTTATCAGTTTC
CACTCTTTGGAAGACCGAATAGTCAAACAATTTATGAGAGATAAAGAGCAAGGTAACAGGCCACCTGTTGAAGTTCCAAT
CAAATACGAAGAACTAAAGACCAATTTTAAAAAAGTAGGTAAAGCAGTGAAACCACAGAGTAGCGAAATAAAGGAAAATG
TTAGATCTCGAAGTGCAGTACTTAGGATAGGGGAGAAATTAGCATGA

Upstream 100 bases:

>100_bases
TATGGGAATCCAAGCGTGAGCAATGGCTGGCAGAAGAAGCGTCCATGACGGATGGGTTACCCGAGGAAATGAAAACGTTT
TCTTTATAACGGAAAGAGAA

Downstream 100 bases:

>100_bases
ACGCTGCAGCGAAAGTCATTAATCAAGGTAATTTATTTAATGGTCAATTGGCAGATATGCAAATGTCAAAATCACTCTAT
ATGTTGATAGTATTGTTAGC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MAKHQSVLLHESIKGLAIKADGIYFDGTFGRGGHSREILNHLSDKGRLFAIDKDLDAVQYAKDYFGLDKRFQIFHGSFAQ
IKEFASQAGVIGAVDGILLDLGVSSPQLDNPERGFSFMLQGPLDMRMDLTQSINAANFVNEAEVNELAHVFRAYGEERFA
GRIAKAIVDARKLKPITTTLELAEIVKEANPKWEKHKHPATRVFQAIRIHVNQELTDLSNCLEQCLDVLGPGGRLAVISF
HSLEDRIVKQFMRDKEQGNRPPVEVPIKYEELKTNFKKVGKAVKPQSSEIKENVRSRSAVLRIGEKLA

Sequences:

>Translated_308_residues
MAKHQSVLLHESIKGLAIKADGIYFDGTFGRGGHSREILNHLSDKGRLFAIDKDLDAVQYAKDYFGLDKRFQIFHGSFAQ
IKEFASQAGVIGAVDGILLDLGVSSPQLDNPERGFSFMLQGPLDMRMDLTQSINAANFVNEAEVNELAHVFRAYGEERFA
GRIAKAIVDARKLKPITTTLELAEIVKEANPKWEKHKHPATRVFQAIRIHVNQELTDLSNCLEQCLDVLGPGGRLAVISF
HSLEDRIVKQFMRDKEQGNRPPVEVPIKYEELKTNFKKVGKAVKPQSSEIKENVRSRSAVLRIGEKLA
>Mature_307_residues
AKHQSVLLHESIKGLAIKADGIYFDGTFGRGGHSREILNHLSDKGRLFAIDKDLDAVQYAKDYFGLDKRFQIFHGSFAQI
KEFASQAGVIGAVDGILLDLGVSSPQLDNPERGFSFMLQGPLDMRMDLTQSINAANFVNEAEVNELAHVFRAYGEERFAG
RIAKAIVDARKLKPITTTLELAEIVKEANPKWEKHKHPATRVFQAIRIHVNQELTDLSNCLEQCLDVLGPGGRLAVISFH
SLEDRIVKQFMRDKEQGNRPPVEVPIKYEELKTNFKKVGKAVKPQSSEIKENVRSRSAVLRIGEKLA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=343, Percent_Identity=39.9416909620991, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI165377202, Length=199, Percent_Identity=43.7185929648241, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1786270, Length=308, Percent_Identity=54.2207792207792, Blast_Score=332, Evalue=2e-92,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=35.8433734939759, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=35.8433734939759, Blast_Score=171, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_LEGPA (Q5X6J0)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123303.1
- ProteinModelPortal:   Q5X6J0
- SMR:   Q5X6J0
- STRING:   Q5X6J0
- GeneID:   3116568
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp0975
- LegioList:   lpp0975
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   LPNE297246:LPP0975-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34461; Mature: 34330

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 78-78 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHQSVLLHESIKGLAIKADGIYFDGTFGRGGHSREILNHLSDKGRLFAIDKDLDAVQY
CCCCHHHHHHHHCCCEEEEECCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHH
AKDYFGLDKRFQIFHGSFAQIKEFASQAGVIGAVDGILLDLGVSSPQLDNPERGFSFMLQ
HHHHHCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEC
GPLDMRMDLTQSINAANFVNEAEVNELAHVFRAYGEERFAGRIAKAIVDARKLKPITTTL
CCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHH
ELAEIVKEANPKWEKHKHPATRVFQAIRIHVNQELTDLSNCLEQCLDVLGPGGRLAVISF
HHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEH
HSLEDRIVKQFMRDKEQGNRPPVEVPIKYEELKTNFKKVGKAVKPQSSEIKENVRSRSAV
HHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LRIGEKLA
HHHHHHCC
>Mature Secondary Structure 
AKHQSVLLHESIKGLAIKADGIYFDGTFGRGGHSREILNHLSDKGRLFAIDKDLDAVQY
CCCHHHHHHHHCCCEEEEECCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHH
AKDYFGLDKRFQIFHGSFAQIKEFASQAGVIGAVDGILLDLGVSSPQLDNPERGFSFMLQ
HHHHHCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEC
GPLDMRMDLTQSINAANFVNEAEVNELAHVFRAYGEERFAGRIAKAIVDARKLKPITTTL
CCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHH
ELAEIVKEANPKWEKHKHPATRVFQAIRIHVNQELTDLSNCLEQCLDVLGPGGRLAVISF
HHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEH
HSLEDRIVKQFMRDKEQGNRPPVEVPIKYEELKTNFKKVGKAVKPQSSEIKENVRSRSAV
HHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LRIGEKLA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA