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The map label for this gene is ppsA [H]

Identifier: 54296826

GI number: 54296826

Start: 974558

End: 976945

Strand: Direct

Name: ppsA [H]

Synonym: lpp0867

Alternate gene names: 54296826

Gene position: 974558-976945 (Clockwise)

Preceding gene: 54296823

Following gene: 54296827

Centisome position: 27.82

GC content: 41.25

Gene sequence:

>2388_bases
ATGACTGTTAAAAGACATACTATAGATTTGGCACATCTTGGCATGCGCGATTTGGATCAGGTCGGTGGTAAGAACTCTTC
TCTTGGTGAAATGATTAGTCATTTGTCATCAGCAGGCGTTTCTGTGCCGGGAGGATTTGCTACAACTGCTGATTCTTTCA
GAGAATTTTTGTCCCAAAACGATCTTGATAAGAAAATTTATGATAAGTTGACTGCTCTCGATACGGATGATGTCTGCCAA
CTGGCAGTGGTAGGGAAACAAATTAGGGAAATGATTATTGATACGCCATTCACCCCGGAGTTTGAACAGTCGGTTCGCTC
TTCCTACCAGAAACTTGCTCAAGAGATTGGGCATGATAATTTTAGTGTTGCAGTAAGATCTTCTGCTACTGCTGAAGATT
TGCCTGATGCTTCTTTTGCCGGTCAGCAGGAAACTTTTTTAAATGTGAAGGGAGAAGAGGCGGTATTGGCTGCCATTAAA
CAGGTATATGCTTCTTTATTTAATGACAGGGCAATAGCTTATCGTGTGCATCATAATTTTGCTCATAACGAAGTGGCATT
GTCTGCTGGCATTCAGCAAATGATTCGTAGTGATTTGGCAGTCAGTGGTGTCATGTTTACTATGGATACTGAATCCGGTT
TTGATCAAGTAGTATTTATCACTTCCTCTTATGGTTTAGGTGAAATGGTTGTTCAAGGCGCTGTAAATCCAGATGAATTT
TATGTCCATAAACCATGCCTTGAAGCCGGAAAACCGGCGATTATCCGTCGAAACCTGGGCAGCAAGGCGTTAAAGATGGT
TTATTGCGATGACCCTTCCCTTGAAAGACGTGTTAAAACAGTCGATGTAGACCCTGCTGAAAGATTATTGTTTTCCTTAT
CCAAAGAGGAAGTAGAGCAATTAGCGAATCAGGCACTTATTATTGAAAAACATTATGGTCGTCCCATGGATATTGAATGG
GCGAAAGACGGGGTCAATGGTAAACTGTATATCCTGCAAGCTCGTCCGGAGACTGTAAAAAGCAGGGATAACAAACAAGT
GCTCGAACGGTATACTTTGCAAAAAAGAGGCGACGTATTGGCTGAAGGCCGTAGTATAGGTCAAAGAATCGGTCAGGGTA
AAGCAAAGGTTATTAAAGATATTAATGAAATGCACAGGGTTCAACCCGGCGATGTTTTGATATCCGATATGACCGATCCT
GATTGGGAACCTGTCATGAAGCGTGCTTCTGCAATCGTTACCAATCGTGGAGGCAGAACTTGTCATGCAGCTATTATTGC
TCGTGAATTGGGGATTCCTGCAGTTGTTGGGTGTGGTGATGCAACAAAAACGATTAAAGATGGTGATGTGGTGACTGTAA
GCTGTGCGGAAGGCGATACAGGTTTTGTCTATTCTGGTCTGTTGCCTTACGAGCAAGAGCGTCTTGATGTAGAAACCATG
CCCGAATTACCCATGAAAGTGATGTTGAATGTTGGAAACCCTGAAAGAGCCTTCGCATTTCAATCAATACCTAATTCCGG
AGTGGGATTGGCCCGTCTTGAATTTTTGATTTCCAACACGATAGGTATTCATCCTAAAGCGCTTCTGGAATTTGATACTT
TAAAGGATGAGGAACTTAAGCGGTACATTAAAGAAAAGACCATAGCGTATGATTCACCTGTAGAATATTACATTGAAAGA
TTAAAAGAAGGCATAGCGACTATTGCTGCTGCTTTTTATCCCAAACCCGTTATAGTCAGGCTTTCAGATTTTAAATCGAA
TGAATACGCTAATCTGGTTGGTGGCTCATTGTATGAACCCCAAGAAGAAAATCCTATGCTTGGTTTTCGTGGCGCCTCTC
GTTATGTTTCTTCAAATTTTTCTGAGTGCTTTGCTCTTGAATGTAAAGCAGTAAGACGAGTCAGGGAGGAGATGGGTTTA
GATAATGTTGAGGTCATGATTCCTTTTGTCCGAACAGTTTCTGAAGCTGGTAATGTCATCGAAGTATTAAAAAAGCATGG
TCTGGAAAGAGGAAAATGTGGGTTAAGAGTGATTATGATGTGCGAGTTACCTTCCAATGCCTTGTTAGCCAGTGAATTTT
TGCAGTATTTTGATGGTTTTTCCATAGGATCGAATGATTTAACTCAATTGACTTTAGGATTAGACAGGGATTCAGGTTTG
GTGGCTTCACAATTTGATGAGCGCAACGATGCTGTGAAAGCTTTATTGCATATGGCTATTTCAGCTTGTAAAAAAGAAGG
CAAGTATATAGGTATTTGCGGTCAAGGTCCTTCAGATCACCAGGATTTTGCCCAATGGCTGATGAAGGAAGGAATTGAGA
GTGTTTCTCTAAACCCTGACTCTGTTTTACAAACATGTTTGTTTTTAGCTAAACAATCGATGAATTGA

Upstream 100 bases:

>100_bases
TATTTTGACCTTTATAAAAATGAAATACAGCACAAGTTCTAATTCCAATAAAGTAACAATTTAAGTAGAATGTTTACTAT
TTTATGAATTTGGGGCGGTT

Downstream 100 bases:

>100_bases
TGAACCAGAGTGTGCTGGCAATTCAGTTTGAAATTGTTAGGCTGTTGGTGCATTAGGAATTTACTGAGGCATTCCTATGA
TTTTGAAATGCAGCAGATTG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 795; Mature: 794

Protein sequence:

>795_residues
MTVKRHTIDLAHLGMRDLDQVGGKNSSLGEMISHLSSAGVSVPGGFATTADSFREFLSQNDLDKKIYDKLTALDTDDVCQ
LAVVGKQIREMIIDTPFTPEFEQSVRSSYQKLAQEIGHDNFSVAVRSSATAEDLPDASFAGQQETFLNVKGEEAVLAAIK
QVYASLFNDRAIAYRVHHNFAHNEVALSAGIQQMIRSDLAVSGVMFTMDTESGFDQVVFITSSYGLGEMVVQGAVNPDEF
YVHKPCLEAGKPAIIRRNLGSKALKMVYCDDPSLERRVKTVDVDPAERLLFSLSKEEVEQLANQALIIEKHYGRPMDIEW
AKDGVNGKLYILQARPETVKSRDNKQVLERYTLQKRGDVLAEGRSIGQRIGQGKAKVIKDINEMHRVQPGDVLISDMTDP
DWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATKTIKDGDVVTVSCAEGDTGFVYSGLLPYEQERLDVETM
PELPMKVMLNVGNPERAFAFQSIPNSGVGLARLEFLISNTIGIHPKALLEFDTLKDEELKRYIKEKTIAYDSPVEYYIER
LKEGIATIAAAFYPKPVIVRLSDFKSNEYANLVGGSLYEPQEENPMLGFRGASRYVSSNFSECFALECKAVRRVREEMGL
DNVEVMIPFVRTVSEAGNVIEVLKKHGLERGKCGLRVIMMCELPSNALLASEFLQYFDGFSIGSNDLTQLTLGLDRDSGL
VASQFDERNDAVKALLHMAISACKKEGKYIGICGQGPSDHQDFAQWLMKEGIESVSLNPDSVLQTCLFLAKQSMN

Sequences:

>Translated_795_residues
MTVKRHTIDLAHLGMRDLDQVGGKNSSLGEMISHLSSAGVSVPGGFATTADSFREFLSQNDLDKKIYDKLTALDTDDVCQ
LAVVGKQIREMIIDTPFTPEFEQSVRSSYQKLAQEIGHDNFSVAVRSSATAEDLPDASFAGQQETFLNVKGEEAVLAAIK
QVYASLFNDRAIAYRVHHNFAHNEVALSAGIQQMIRSDLAVSGVMFTMDTESGFDQVVFITSSYGLGEMVVQGAVNPDEF
YVHKPCLEAGKPAIIRRNLGSKALKMVYCDDPSLERRVKTVDVDPAERLLFSLSKEEVEQLANQALIIEKHYGRPMDIEW
AKDGVNGKLYILQARPETVKSRDNKQVLERYTLQKRGDVLAEGRSIGQRIGQGKAKVIKDINEMHRVQPGDVLISDMTDP
DWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATKTIKDGDVVTVSCAEGDTGFVYSGLLPYEQERLDVETM
PELPMKVMLNVGNPERAFAFQSIPNSGVGLARLEFLISNTIGIHPKALLEFDTLKDEELKRYIKEKTIAYDSPVEYYIER
LKEGIATIAAAFYPKPVIVRLSDFKSNEYANLVGGSLYEPQEENPMLGFRGASRYVSSNFSECFALECKAVRRVREEMGL
DNVEVMIPFVRTVSEAGNVIEVLKKHGLERGKCGLRVIMMCELPSNALLASEFLQYFDGFSIGSNDLTQLTLGLDRDSGL
VASQFDERNDAVKALLHMAISACKKEGKYIGICGQGPSDHQDFAQWLMKEGIESVSLNPDSVLQTCLFLAKQSMN
>Mature_794_residues
TVKRHTIDLAHLGMRDLDQVGGKNSSLGEMISHLSSAGVSVPGGFATTADSFREFLSQNDLDKKIYDKLTALDTDDVCQL
AVVGKQIREMIIDTPFTPEFEQSVRSSYQKLAQEIGHDNFSVAVRSSATAEDLPDASFAGQQETFLNVKGEEAVLAAIKQ
VYASLFNDRAIAYRVHHNFAHNEVALSAGIQQMIRSDLAVSGVMFTMDTESGFDQVVFITSSYGLGEMVVQGAVNPDEFY
VHKPCLEAGKPAIIRRNLGSKALKMVYCDDPSLERRVKTVDVDPAERLLFSLSKEEVEQLANQALIIEKHYGRPMDIEWA
KDGVNGKLYILQARPETVKSRDNKQVLERYTLQKRGDVLAEGRSIGQRIGQGKAKVIKDINEMHRVQPGDVLISDMTDPD
WEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATKTIKDGDVVTVSCAEGDTGFVYSGLLPYEQERLDVETMP
ELPMKVMLNVGNPERAFAFQSIPNSGVGLARLEFLISNTIGIHPKALLEFDTLKDEELKRYIKEKTIAYDSPVEYYIERL
KEGIATIAAAFYPKPVIVRLSDFKSNEYANLVGGSLYEPQEENPMLGFRGASRYVSSNFSECFALECKAVRRVREEMGLD
NVEVMIPFVRTVSEAGNVIEVLKKHGLERGKCGLRVIMMCELPSNALLASEFLQYFDGFSIGSNDLTQLTLGLDRDSGLV
ASQFDERNDAVKALLHMAISACKKEGKYIGICGQGPSDHQDFAQWLMKEGIESVSLNPDSVLQTCLFLAKQSMN

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=780, Percent_Identity=68.8461538461538, Blast_Score=1137, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=462, Percent_Identity=25.3246753246753, Blast_Score=123, Evalue=6e-29,
Organism=Escherichia coli, GI1788726, Length=190, Percent_Identity=31.0526315789474, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789193, Length=413, Percent_Identity=25.4237288135593, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI48994992, Length=402, Percent_Identity=24.3781094527363, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17564524, Length=333, Percent_Identity=26.1261261261261, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87827; Mature: 87696

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKRHTIDLAHLGMRDLDQVGGKNSSLGEMISHLSSAGVSVPGGFATTADSFREFLSQN
CCCCCCCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
DLDKKIYDKLTALDTDDVCQLAVVGKQIREMIIDTPFTPEFEQSVRSSYQKLAQEIGHDN
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
FSVAVRSSATAEDLPDASFAGQQETFLNVKGEEAVLAAIKQVYASLFNDRAIAYRVHHNF
EEEEEECCCCHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCC
AHNEVALSAGIQQMIRSDLAVSGVMFTMDTESGFDQVVFITSSYGLGEMVVQGAVNPDEF
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCCCCE
YVHKPCLEAGKPAIIRRNLGSKALKMVYCDDPSLERRVKTVDVDPAERLLFSLSKEEVEQ
EEECCHHHCCCCEEEECCCCCCCEEEEECCCCCHHHHHEECCCCHHHHHHHHCCHHHHHH
LANQALIIEKHYGRPMDIEWAKDGVNGKLYILQARPETVKSRDNKQVLERYTLQKRGDVL
HHCCEEEEEECCCCCCCCCHHCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHCCCHH
AEGRSIGQRIGQGKAKVIKDINEMHRVQPGDVLISDMTDPDWEPVMKRASAIVTNRGGRT
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCH
CHAAIIARELGIPAVVGCGDATKTIKDGDVVTVSCAEGDTGFVYSGLLPYEQERLDVETM
HHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCHHHHCCHHHC
PELPMKVMLNVGNPERAFAFQSIPNSGVGLARLEFLISNTIGIHPKALLEFDTLKDEELK
CCCCEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
RYIKEKTIAYDSPVEYYIERLKEGIATIAAAFYPKPVIVRLSDFKSNEYANLVGGSLYEP
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCCCC
QEENPMLGFRGASRYVSSNFSECFALECKAVRRVREEMGLDNVEVMIPFVRTVSEAGNVI
CCCCCCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EVLKKHGLERGKCGLRVIMMCELPSNALLASEFLQYFDGFSIGSNDLTQLTLGLDRDSGL
HHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCC
VASQFDERNDAVKALLHMAISACKKEGKYIGICGQGPSDHQDFAQWLMKEGIESVSLNPD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHH
SVLQTCLFLAKQSMN
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVKRHTIDLAHLGMRDLDQVGGKNSSLGEMISHLSSAGVSVPGGFATTADSFREFLSQN
CCCCCCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
DLDKKIYDKLTALDTDDVCQLAVVGKQIREMIIDTPFTPEFEQSVRSSYQKLAQEIGHDN
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
FSVAVRSSATAEDLPDASFAGQQETFLNVKGEEAVLAAIKQVYASLFNDRAIAYRVHHNF
EEEEEECCCCHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCC
AHNEVALSAGIQQMIRSDLAVSGVMFTMDTESGFDQVVFITSSYGLGEMVVQGAVNPDEF
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCCCCE
YVHKPCLEAGKPAIIRRNLGSKALKMVYCDDPSLERRVKTVDVDPAERLLFSLSKEEVEQ
EEECCHHHCCCCEEEECCCCCCCEEEEECCCCCHHHHHEECCCCHHHHHHHHCCHHHHHH
LANQALIIEKHYGRPMDIEWAKDGVNGKLYILQARPETVKSRDNKQVLERYTLQKRGDVL
HHCCEEEEEECCCCCCCCCHHCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHCCCHH
AEGRSIGQRIGQGKAKVIKDINEMHRVQPGDVLISDMTDPDWEPVMKRASAIVTNRGGRT
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCH
CHAAIIARELGIPAVVGCGDATKTIKDGDVVTVSCAEGDTGFVYSGLLPYEQERLDVETM
HHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCHHHHCCHHHC
PELPMKVMLNVGNPERAFAFQSIPNSGVGLARLEFLISNTIGIHPKALLEFDTLKDEELK
CCCCEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
RYIKEKTIAYDSPVEYYIERLKEGIATIAAAFYPKPVIVRLSDFKSNEYANLVGGSLYEP
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCCCC
QEENPMLGFRGASRYVSSNFSECFALECKAVRRVREEMGLDNVEVMIPFVRTVSEAGNVI
CCCCCCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EVLKKHGLERGKCGLRVIMMCELPSNALLASEFLQYFDGFSIGSNDLTQLTLGLDRDSGL
HHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCC
VASQFDERNDAVKALLHMAISACKKEGKYIGICGQGPSDHQDFAQWLMKEGIESVSLNPD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHH
SVLQTCLFLAKQSMN
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]