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The map label for this gene is epsN [H]

Identifier: 54296782

GI number: 54296782

Start: 918268

End: 919770

Strand: Direct

Name: epsN [H]

Synonym: lpp0821

Alternate gene names: 54296782

Gene position: 918268-919770 (Clockwise)

Preceding gene: 54296781

Following gene: 54296783

Centisome position: 26.21

GC content: 38.92

Gene sequence:

>1503_bases
ATGATTACTATCAAAGAGTTATATATACAAAAACAAGAAAATCTGAAAACTGCTTTGAGCAAGCTGGATGCTACAGCTCA
AGGTGTGTTATTTCTGGTAGATAGTGATGAACATTTAATAAGGACAGTTACAGATGGTGACATCCGAAGATTGCTATTAA
AAGGATTTACATTAGATTCTACTTTAGAGGAATTATCAGAGCATTCATCAAAAGCTTTACCTGTCTCTGCTACGATTCAG
GATGCTTATCATTTGATGCAGGAATATGAATTAGATCATATTCCCGTTATCGATGAGAGTAATCGCCCCATACGCTTGAT
CCACCGGAGAGAACTCTCTTCCAATATTCTGCTTTCCTCGCCTCATATCGGTGAGCATGAGCAGCAATACGTACAGGAAG
CTTTTGCCACCAACTGGGTAGCCCCTTTAGGCCCCAATGTTGATTCTTTTGAAAAAGAAGTGGCGGAATACATTAATATA
AAATCAGCTGTGGCTTTAAGCTCAGGAACAGCTGCAATTCATCTGGCACTCGTATTACTCGATGTGAAACCAGGAGATAT
TGTGTTCGCATCCAGCTTTACTTTTGTAGCCACAGTGAATCCTATCTTATACCAAGGTGCGACTCCGGTATTTATTGATT
CTGATTTGGACACCTGGAATATGTCTCCAGTTGCTCTTGAGCGGGCATTAAGAGAAAGTAAAGCAAAAAATCAAATGCCA
AAAGCTGTGATTATTGTTAATTTATATGGACAAAGTGCTAATTATGACGCTTTATGTCAATTATGCAATGAATACAATGT
GCCAATTATAGAAGATGCGGCGGAGTCTTTGGGAGCCACCTATAATAACAAGTACAGCGGGACTTTCGGCAAGTTAGGCG
TGTTTTCATTTAATGGCAACAAAATCATTACCACGTCTGGTGGCGGCATGTTGGTGTCTGAAGATGAGAGTCTCATACAA
AGAGCAAGATTTTTATCAACTCAGGCTCGCGAACCAATGCCCCATTATGAACATACTGTGGTTGGGTATAATTACAGAAT
GAGTAATGTGCTTGCTGGTATAGGCAGAGGTCAGCTGAAAGTATTGGAAAAACGGGTTAAGGCAAGACGAGCTATTTTTG
AACGATATAAGCAAGCCTTGGAATCTTACCCATTTATTGACATGATGCCAGAGATAACCCAAGGATACAGTACTCGTTGG
TTATCGACATTGGTTATTAAACCAACGCCAAACAAGATAAAACCGGAAGAAGTCATTGAGCAATTAAAGCCATTTAACAT
TGAAGCAAGAAGAACATGGAAGCCTATGCATAGACAGCCTTTATTTGCAGGCTCTAAATACTATCCTCATGATGAACAAT
TCAGCGTTTCAGATTACTTGTTTGATCATGGTATTTGTCTTCCTTCCGGGTCAAATTTAACCCCCAGAGACATTGATAGA
GTCATTCATTGTTTGAATGATATTTTTATTAAATATAAACAAAACAATTTTGAGGTAGCTTAA

Upstream 100 bases:

>100_bases
ATAGGTGATGGAGCAATAATTGGAGCTGGCTCAGTCGTTGTGAAGGATGTAAAAGAAAATGCTGTTGTGAAAGGCGTACC
CGCAGTTTAAAAGGAATAAA

Downstream 100 bases:

>100_bases
TTATTTGTTCTAGTGAAAACTGGATACAGGTAGTAAAAGATTTAAATATTAAGGAATTATAATTCTCTTTTAAGAGCTTT
AGTAACGATATGAATATAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MITIKELYIQKQENLKTALSKLDATAQGVLFLVDSDEHLIRTVTDGDIRRLLLKGFTLDSTLEELSEHSSKALPVSATIQ
DAYHLMQEYELDHIPVIDESNRPIRLIHRRELSSNILLSSPHIGEHEQQYVQEAFATNWVAPLGPNVDSFEKEVAEYINI
KSAVALSSGTAAIHLALVLLDVKPGDIVFASSFTFVATVNPILYQGATPVFIDSDLDTWNMSPVALERALRESKAKNQMP
KAVIIVNLYGQSANYDALCQLCNEYNVPIIEDAAESLGATYNNKYSGTFGKLGVFSFNGNKIITTSGGGMLVSEDESLIQ
RARFLSTQAREPMPHYEHTVVGYNYRMSNVLAGIGRGQLKVLEKRVKARRAIFERYKQALESYPFIDMMPEITQGYSTRW
LSTLVIKPTPNKIKPEEVIEQLKPFNIEARRTWKPMHRQPLFAGSKYYPHDEQFSVSDYLFDHGICLPSGSNLTPRDIDR
VIHCLNDIFIKYKQNNFEVA

Sequences:

>Translated_500_residues
MITIKELYIQKQENLKTALSKLDATAQGVLFLVDSDEHLIRTVTDGDIRRLLLKGFTLDSTLEELSEHSSKALPVSATIQ
DAYHLMQEYELDHIPVIDESNRPIRLIHRRELSSNILLSSPHIGEHEQQYVQEAFATNWVAPLGPNVDSFEKEVAEYINI
KSAVALSSGTAAIHLALVLLDVKPGDIVFASSFTFVATVNPILYQGATPVFIDSDLDTWNMSPVALERALRESKAKNQMP
KAVIIVNLYGQSANYDALCQLCNEYNVPIIEDAAESLGATYNNKYSGTFGKLGVFSFNGNKIITTSGGGMLVSEDESLIQ
RARFLSTQAREPMPHYEHTVVGYNYRMSNVLAGIGRGQLKVLEKRVKARRAIFERYKQALESYPFIDMMPEITQGYSTRW
LSTLVIKPTPNKIKPEEVIEQLKPFNIEARRTWKPMHRQPLFAGSKYYPHDEQFSVSDYLFDHGICLPSGSNLTPRDIDR
VIHCLNDIFIKYKQNNFEVA
>Mature_500_residues
MITIKELYIQKQENLKTALSKLDATAQGVLFLVDSDEHLIRTVTDGDIRRLLLKGFTLDSTLEELSEHSSKALPVSATIQ
DAYHLMQEYELDHIPVIDESNRPIRLIHRRELSSNILLSSPHIGEHEQQYVQEAFATNWVAPLGPNVDSFEKEVAEYINI
KSAVALSSGTAAIHLALVLLDVKPGDIVFASSFTFVATVNPILYQGATPVFIDSDLDTWNMSPVALERALRESKAKNQMP
KAVIIVNLYGQSANYDALCQLCNEYNVPIIEDAAESLGATYNNKYSGTFGKLGVFSFNGNKIITTSGGGMLVSEDESLIQ
RARFLSTQAREPMPHYEHTVVGYNYRMSNVLAGIGRGQLKVLEKRVKARRAIFERYKQALESYPFIDMMPEITQGYSTRW
LSTLVIKPTPNKIKPEEVIEQLKPFNIEARRTWKPMHRQPLFAGSKYYPHDEQFSVSDYLFDHGICLPSGSNLTPRDIDR
VIHCLNDIFIKYKQNNFEVA

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=394, Percent_Identity=29.6954314720812, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI2367285, Length=273, Percent_Identity=27.8388278388278, Blast_Score=118, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 56408; Mature: 56408

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIKELYIQKQENLKTALSKLDATAQGVLFLVDSDEHLIRTVTDGDIRRLLLKGFTLDS
CEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHCCCCHH
TLEELSEHSSKALPVSATIQDAYHLMQEYELDHIPVIDESNRPIRLIHRRELSSNILLSS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEHHHCCCCEEEEC
PHIGEHEQQYVQEAFATNWVAPLGPNVDSFEKEVAEYINIKSAVALSSGTAAIHLALVLL
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHEEECCCCHHEEEEEEEE
DVKPGDIVFASSFTFVATVNPILYQGATPVFIDSDLDTWNMSPVALERALRESKAKNQMP
ECCCCCEEEECCEEEEEECCHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
KAVIIVNLYGQSANYDALCQLCNEYNVPIIEDAAESLGATYNNKYSGTFGKLGVFSFNGN
CEEEEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHCCCCCCCCCCCCCCCEEEEEECCC
KIITTSGGGMLVSEDESLIQRARFLSTQAREPMPHYEHTVVGYNYRMSNVLAGIGRGQLK
EEEEECCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEECEEHHHHHHHCCCHHHH
VLEKRVKARRAIFERYKQALESYPFIDMMPEITQGYSTRWLSTLVIKPTPNKIKPEEVIE
HHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCCCEEEEEEEEECCCCCCCHHHHHH
QLKPFNIEARRTWKPMHRQPLFAGSKYYPHDEQFSVSDYLFDHGICLPSGSNLTPRDIDR
HHCCCCCCHHCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHCCEECCCCCCCCHHHHHH
VIHCLNDIFIKYKQNNFEVA
HHHHHHHHHEEEECCCCCCC
>Mature Secondary Structure
MITIKELYIQKQENLKTALSKLDATAQGVLFLVDSDEHLIRTVTDGDIRRLLLKGFTLDS
CEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHCCCCHH
TLEELSEHSSKALPVSATIQDAYHLMQEYELDHIPVIDESNRPIRLIHRRELSSNILLSS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEHHHCCCCEEEEC
PHIGEHEQQYVQEAFATNWVAPLGPNVDSFEKEVAEYINIKSAVALSSGTAAIHLALVLL
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHEEECCCCHHEEEEEEEE
DVKPGDIVFASSFTFVATVNPILYQGATPVFIDSDLDTWNMSPVALERALRESKAKNQMP
ECCCCCEEEECCEEEEEECCHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
KAVIIVNLYGQSANYDALCQLCNEYNVPIIEDAAESLGATYNNKYSGTFGKLGVFSFNGN
CEEEEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHCCCCCCCCCCCCCCCEEEEEECCC
KIITTSGGGMLVSEDESLIQRARFLSTQAREPMPHYEHTVVGYNYRMSNVLAGIGRGQLK
EEEEECCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEECEEHHHHHHHCCCHHHH
VLEKRVKARRAIFERYKQALESYPFIDMMPEITQGYSTRWLSTLVIKPTPNKIKPEEVIE
HHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCCCEEEEEEEEECCCCCCCHHHHHH
QLKPFNIEARRTWKPMHRQPLFAGSKYYPHDEQFSVSDYLFDHGICLPSGSNLTPRDIDR
HHCCCCCCHHCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHCCEECCCCCCCCHHHHHH
VIHCLNDIFIKYKQNNFEVA
HHHHHHHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]