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The map label for this gene is hisH

Identifier: 54296777

GI number: 54296777

Start: 914155

End: 914796

Strand: Direct

Name: hisH

Synonym: lpp0816

Alternate gene names: 54296777

Gene position: 914155-914796 (Clockwise)

Preceding gene: 54296776

Following gene: 54296778

Centisome position: 26.09

GC content: 39.25

Gene sequence:

>642_bases
ATGAGTTCAGTTTCAATTGTAGATTATGGGGTAGGCAATTTATTAAGTGTGGCTCGAGCTTTTCAATATTTTGATGCTTC
TGTCAATTTAGTGAGCACACCCGAGGAAATTATGAGTGCCGATCGGTTGGTTTTGCCAGGAGTAGGCGCTTTTGAAGATG
GGATGAAAGGATTAACTACATTAAATTTTATAGAACCCATAAAGCAATTTGCGCGGAGTGGAAAACCTTTTCTGGGAATA
TGTCTTGGCATGCAGATGATGCTCTCAAGAAGTACTGAATTCGGACAACATGAAGGCCTGGATCTAATAGCTGGCGAGGT
TGTTAGCGTTCCTTCACATGGAGTTGATGGTCAATTGCATAAAATACCTCATATAGGCTGGAATGAACTGGTTTCCACCT
CAGAAGGCGAGGATTGGTGTCATACTATCCTGAAAAATATACCATTAAATTCTTCAGTATATTTTGTTCATTCTTTTATG
GCCATGCCTAGTAATCCTAAAAAGCGTCTGGCTGATACTTTATATGATGGTCAGGCTATTAGCGCAGTAATAAAAGATGA
AAATATGTACGGATGCCAATTTCATCCTGAAAAAAGCGGGGAAGTAGGTTTAAGCATCATTCAACAGTTTTTGCAGATTT
AG

Upstream 100 bases:

>100_bases
CTGATGCAGTCGCTATGGCGGATGTTTTGCATTACAAAAGAATAGGTTTACCTGAAATCAGAAAGTATGCTTTAGATAAT
GGCATTCATGTGAGAGTCTT

Downstream 100 bases:

>100_bases
GGTGAATTAAAAATGAGAATATTGGCAGTAATCCCGGCAAGGGCTGGCTCAAAGAGGCTGCCCGGTAAAAATACCAGATT
ACTTGCCGGAAAGCCATTAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTTLNFIEPIKQFARSGKPFLGI
CLGMQMMLSRSTEFGQHEGLDLIAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFM
AMPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI

Sequences:

>Translated_213_residues
MSSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTTLNFIEPIKQFARSGKPFLGI
CLGMQMMLSRSTEFGQHEGLDLIAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFM
AMPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI
>Mature_212_residues
SSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTTLNFIEPIKQFARSGKPFLGIC
LGMQMMLSRSTEFGQHEGLDLIAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFMA
MPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=211, Percent_Identity=33.175355450237, Blast_Score=110, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6319725, Length=222, Percent_Identity=31.5315315315315, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS51_LEGPA (Q5X6Z7)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123146.1
- ProteinModelPortal:   Q5X6Z7
- STRING:   Q5X6Z7
- GeneID:   3117290
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp0816
- LegioList:   lpp0816
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   ASENFED
- ProtClustDB:   PRK13146
- BioCyc:   LPNE297246:LPP0816-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23310; Mature: 23179

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 195-195 ACT_SITE 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTT
CCCCEEECCCHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCEECCCCCCHHHHHHHHHH
LNFIEPIKQFARSGKPFLGICLGMQMMLSRSTEFGQHEGLDLIAGEVVSVPSHGVDGQLH
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEHCCCCCCCCCCHH
KIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFMAMPSNPKKRLADTLYDGQAI
CCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHH
SAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI
HHEECCCCEECCEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTT
CCCEEECCCHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCEECCCCCCHHHHHHHHHH
LNFIEPIKQFARSGKPFLGICLGMQMMLSRSTEFGQHEGLDLIAGEVVSVPSHGVDGQLH
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEHCCCCCCCCCCHH
KIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFMAMPSNPKKRLADTLYDGQAI
CCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHH
SAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI
HHEECCCCEECCEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA