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The map label for this gene is thyA [H]

Identifier: 54295693

GI number: 54295693

Start: 3178743

End: 3179537

Strand: Reverse

Name: thyA [H]

Synonym: lpl2781

Alternate gene names: 54295693

Gene position: 3179537-3178743 (Counterclockwise)

Preceding gene: 54295694

Following gene: 54295692

Centisome position: 95.03

GC content: 39.5

Gene sequence:

>795_bases
ATGAAAACATATTTGCAATTGTTAGAGCATATTTTACAACATGGGGTAGAAAAATCGGATAGAACGGGCACTGGAACATT
ATCTGTATTTGGGTATCAAATGCGTTTTGATTTGGCCAAGGGATTCCCGCTTGTTACCACTAAAAAACTACATACGAGAA
GTATTGTTCATGAATTACTATGGTTTTTGCGTGGTGATACCAATATTTCTTATCTTAAGGAAAATGGGGTAACTATTTGG
GATGAATGGGCAGATAATAATGGTGATTTGGGGCCTGTCTATGGAAAGCAATGGAGAAGTTGGCCCACTGCTGATGGAGG
CACCATTGATCAATTAAGTGATGTAGTGCAACAAATAAAGAGCAACCCTGACTCTCGTCGCTTGATTGTAAGCGCCTGGA
ATGTGGGTGAATTGGATAAAATGGCTTTAATGCCTTGTCATGCCTTGTTTCAGTTTTATGTTGCAAACAACAAGTTATCC
TGTCAATTGTATCAACGCTCAGCCGATGTATTTTTAGGAGTTCCTTTCAATATCGCTTCTTATTCGTTGCTTACTCATAT
GGTCGCCCAACAATGTAATTTGGATGTGGCCGAGTTCATATGGACCGGGGGAGATTGTCATTTGTATCTTAATCATTTGG
AGCAAGCGCAAACTCAATTAACAAGAGAACCTTTGCCATTGCCCTCCTTAGCTATTAAGAGAAAACCGGCTTCTTTATTT
GATTATGCTTACGAGGATTTCGAATTTGTGAACTATCAATCACATCCTGCAATTAAAGCGCCTATCGCCGTTTAA

Upstream 100 bases:

>100_bases
ATTCGCAATTAGGTTTTGTGGCATTTGGCTGGCTGACAATGGGGCAGGTTTTATCCATCCCTATGTTATTAATTGGAATT
TGGTTATGGTGGGCTAAGCG

Downstream 100 bases:

>100_bases
TTTTCATCAGAATTGGATTTTTATTGCCACTTGTGGTTTAATATTGCCTCAAATGTTTGTTAGAGGTTCAATTTCGTGAA
AAAAATTATGGTGTTTGTAT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKTYLQLLEHILQHGVEKSDRTGTGTLSVFGYQMRFDLAKGFPLVTTKKLHTRSIVHELLWFLRGDTNISYLKENGVTIW
DEWADNNGDLGPVYGKQWRSWPTADGGTIDQLSDVVQQIKSNPDSRRLIVSAWNVGELDKMALMPCHALFQFYVANNKLS
CQLYQRSADVFLGVPFNIASYSLLTHMVAQQCNLDVAEFIWTGGDCHLYLNHLEQAQTQLTREPLPLPSLAIKRKPASLF
DYAYEDFEFVNYQSHPAIKAPIAV

Sequences:

>Translated_264_residues
MKTYLQLLEHILQHGVEKSDRTGTGTLSVFGYQMRFDLAKGFPLVTTKKLHTRSIVHELLWFLRGDTNISYLKENGVTIW
DEWADNNGDLGPVYGKQWRSWPTADGGTIDQLSDVVQQIKSNPDSRRLIVSAWNVGELDKMALMPCHALFQFYVANNKLS
CQLYQRSADVFLGVPFNIASYSLLTHMVAQQCNLDVAEFIWTGGDCHLYLNHLEQAQTQLTREPLPLPSLAIKRKPASLF
DYAYEDFEFVNYQSHPAIKAPIAV
>Mature_264_residues
MKTYLQLLEHILQHGVEKSDRTGTGTLSVFGYQMRFDLAKGFPLVTTKKLHTRSIVHELLWFLRGDTNISYLKENGVTIW
DEWADNNGDLGPVYGKQWRSWPTADGGTIDQLSDVVQQIKSNPDSRRLIVSAWNVGELDKMALMPCHALFQFYVANNKLS
CQLYQRSADVFLGVPFNIASYSLLTHMVAQQCNLDVAEFIWTGGDCHLYLNHLEQAQTQLTREPLPLPSLAIKRKPASLF
DYAYEDFEFVNYQSHPAIKAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=50.8896797153025, Blast_Score=280, Evalue=7e-76,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=71.5909090909091, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=45.0354609929078, Blast_Score=256, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6324648, Length=230, Percent_Identity=45.2173913043478, Blast_Score=193, Evalue=2e-50,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=50.530035335689, Blast_Score=277, Evalue=6e-75,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30027; Mature: 30027

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYLQLLEHILQHGVEKSDRTGTGTLSVFGYQMRFDLAKGFPLVTTKKLHTRSIVHELL
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEHHHCCCCCEECHHHHHHHHHHHHH
WFLRGDTNISYLKENGVTIWDEWADNNGDLGPVYGKQWRSWPTADGGTIDQLSDVVQQIK
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHH
SNPDSRRLIVSAWNVGELDKMALMPCHALFQFYVANNKLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YSLLTHMVAQQCNLDVAEFIWTGGDCHLYLNHLEQAQTQLTREPLPLPSLAIKRKPASLF
HHHHHHHHHHHHCCCHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHH
DYAYEDFEFVNYQSHPAIKAPIAV
HHHHHHHHEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKTYLQLLEHILQHGVEKSDRTGTGTLSVFGYQMRFDLAKGFPLVTTKKLHTRSIVHELL
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEHHHCCCCCEECHHHHHHHHHHHHH
WFLRGDTNISYLKENGVTIWDEWADNNGDLGPVYGKQWRSWPTADGGTIDQLSDVVQQIK
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHH
SNPDSRRLIVSAWNVGELDKMALMPCHALFQFYVANNKLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YSLLTHMVAQQCNLDVAEFIWTGGDCHLYLNHLEQAQTQLTREPLPLPSLAIKRKPASLF
HHHHHHHHHHHHCCCHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHH
DYAYEDFEFVNYQSHPAIKAPIAV
HHHHHHHHEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA