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The map label for this gene is rep [H]

Identifier: 54295644

GI number: 54295644

Start: 3123079

End: 3125076

Strand: Reverse

Name: rep [H]

Synonym: lpl2731

Alternate gene names: 54295644

Gene position: 3125076-3123079 (Counterclockwise)

Preceding gene: 54295645

Following gene: 54295642

Centisome position: 93.41

GC content: 39.74

Gene sequence:

>1998_bases
ATGTTGAATAGCCAACAAATGGCTGCAGTAAAATATATTGATGGGCCTTTGTTGGTGCTTGCCGGGGCTGGGAGTGGTAA
AACCAGAGTTATTACACAAAAAATTGGTTATTTAATTAATTCATGTGGTTATAATGCCAACTCCATCTGCGCCGTTACTT
TCACGAATAAAGCGGCGAATGAAATGCGCGCAAGGGTTTCGGCCTTTTTACCAGCCGCAAGCAGGCGGGGATTAAAGGTT
GCGACATTTCATACATTAGGCCTAAGCATCATCAAGCGTGATGTTTCTAGGTGCGATTTGAAACCGGGATTTTCCATTTT
TGATAGTGAAGATTGTTTGCAAATTTTGCGGGGTTTTTTACCTGTTAATAAGGCAACCGATAGAGATTTTATCCTCAAAG
TTCAACAACAAATTTCTCTTTGGAAAAATAATTTATTGAATCCCGAATTAATACTCCAAAACCCTCCGGAAACACTTTTA
TGTGACGAGGCGTTAACAATTTATCCACGCTATCAAGAAGCATTAAAAGCATATAATGCAGTGGACTTTGATGATTTAAT
TCGGTTACCTGTCGATCTACTGAATGAGCATGCTGATGTGTTGGAATATTGGCAGAATAAAATTCGTCATTTGTTGGTGG
ATGAATATCAGGATTCAAACACTAGCCAGTATCTTCTGGTAAAAAAATTGGCAGGGGTTCGTGCCCACTTTACGGTTGTG
GGTGATGATGATCAATCGATTTATGCCTGGCGTGGTGCCAAACCAGAGAATTTGGCGCAGTTACAAAAAGATTTTCCTCG
GTTAAAGATTATTAAATTAGAACAGAATTACAGATCAACCAGCCGAATTTTACATGCTGCTAACCAACTGATCGCTCATA
ATCAACATTTATTTGAAAAAAAATTATGGAGTGATTTGGGGCATGGGGAATTATTAAGGGTTGTCAGCTGTAAGGATGAG
CAGGATGAGGCGGAACATGTGATTGCTGATTTAATAAGCCATAAATTACGTAATCGGACAAAATATAGCGATTACGCCAT
CTTATACAGAGGAAATCATCAAGCAAGAATATTTGAAAAGGTATTAAGACATCACGGTATTCCTTATCATATCAGCGGAG
GCCAGTCCTGGTTTGCCAAGTTAGAGGTCAGGGACATTTTTGCCTATCTTAAATTACTTTGTAATGAAGCAGATGATGCC
GCTTTTCTGCGAGCAATTACTACCCCCAAACGAGGTATAGGAGAGAGCAGTCTCGATGCTTTGGGACGCTATGCTCAGTC
CAGAGGGATAAGTTTATATCACGGGTCCGATCATTTGGGCTTGTCTGAGTTTGTTGCTGACAAACAGAGAATGATTTTTC
ATGATTTCAAATTGTGGATGGAAGACATTAAAAAACGTATCAGTTCAGATTCAGTTCTGGAGCATTTAAGGCAAATGGTG
GAAGATATTGGGTATGAAGCTTATATTTATGAACAATGTGATTCTCCCGCCAAGGCACAAAAAAAAATGGATAATGTATG
GGAGCTTTTAGAATGGGTTAATCGTTTGTTGAATAAGGAACCTGAACAATCGCTCATGGATGTTGTGAATAAATTAATAC
TAATCGATATACTGGAACAATCCGATGAATCTTCAAGTGATGTAGTCCAATTAATGACATTGCATGCCTCAAAAGGTTTG
GAATTTCCTTTTGTTTATTTGGTCGGGATGGAAGAAGAATTATTACCCCACCGAGTGAGTATCGATGATGACCAAATTGA
GGAAGAAAGAAGATTGGCTTATGTAGGGATCACACGTGCCCAGAAGGGATTATGTTTTACTTTAGCGAAGCAGAGACGTC
GAGCTGGAGAACTGCAAGATTGTGTTCCAAGCCGATTTTTGGATGAATTGCCGCAAGAGAGCCTGGAATGGTTCGGTAAA
GGCGGGGAGCGTTGTGAAGAACAATCAAAAAAACTGGCCAAATCCCACCTGGAAGGACTGAAAAATTTGTTGAGTTAA

Upstream 100 bases:

>100_bases
GACAACGAAGACGAGTTTGATGTAGAACGCTCTATCAATTAATTCTTGCGTGAAAAAGAAAGCCTGGGTGATAATGCCTT
TTTTTTGAGCGGGAATAAGT

Downstream 100 bases:

>100_bases
GAGTACATTATATTTTATCGCCAGTAAGGAGTATCATTAACTGAACTCCTTACTGCTTTTTAAGGTACTAATGTTAACCT
CTGTCATTTGCCAGAAGAGA

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MLNSQQMAAVKYIDGPLLVLAGAGSGKTRVITQKIGYLINSCGYNANSICAVTFTNKAANEMRARVSAFLPAASRRGLKV
ATFHTLGLSIIKRDVSRCDLKPGFSIFDSEDCLQILRGFLPVNKATDRDFILKVQQQISLWKNNLLNPELILQNPPETLL
CDEALTIYPRYQEALKAYNAVDFDDLIRLPVDLLNEHADVLEYWQNKIRHLLVDEYQDSNTSQYLLVKKLAGVRAHFTVV
GDDDQSIYAWRGAKPENLAQLQKDFPRLKIIKLEQNYRSTSRILHAANQLIAHNQHLFEKKLWSDLGHGELLRVVSCKDE
QDEAEHVIADLISHKLRNRTKYSDYAILYRGNHQARIFEKVLRHHGIPYHISGGQSWFAKLEVRDIFAYLKLLCNEADDA
AFLRAITTPKRGIGESSLDALGRYAQSRGISLYHGSDHLGLSEFVADKQRMIFHDFKLWMEDIKKRISSDSVLEHLRQMV
EDIGYEAYIYEQCDSPAKAQKKMDNVWELLEWVNRLLNKEPEQSLMDVVNKLILIDILEQSDESSSDVVQLMTLHASKGL
EFPFVYLVGMEEELLPHRVSIDDDQIEEERRLAYVGITRAQKGLCFTLAKQRRRAGELQDCVPSRFLDELPQESLEWFGK
GGERCEEQSKKLAKSHLEGLKNLLS

Sequences:

>Translated_665_residues
MLNSQQMAAVKYIDGPLLVLAGAGSGKTRVITQKIGYLINSCGYNANSICAVTFTNKAANEMRARVSAFLPAASRRGLKV
ATFHTLGLSIIKRDVSRCDLKPGFSIFDSEDCLQILRGFLPVNKATDRDFILKVQQQISLWKNNLLNPELILQNPPETLL
CDEALTIYPRYQEALKAYNAVDFDDLIRLPVDLLNEHADVLEYWQNKIRHLLVDEYQDSNTSQYLLVKKLAGVRAHFTVV
GDDDQSIYAWRGAKPENLAQLQKDFPRLKIIKLEQNYRSTSRILHAANQLIAHNQHLFEKKLWSDLGHGELLRVVSCKDE
QDEAEHVIADLISHKLRNRTKYSDYAILYRGNHQARIFEKVLRHHGIPYHISGGQSWFAKLEVRDIFAYLKLLCNEADDA
AFLRAITTPKRGIGESSLDALGRYAQSRGISLYHGSDHLGLSEFVADKQRMIFHDFKLWMEDIKKRISSDSVLEHLRQMV
EDIGYEAYIYEQCDSPAKAQKKMDNVWELLEWVNRLLNKEPEQSLMDVVNKLILIDILEQSDESSSDVVQLMTLHASKGL
EFPFVYLVGMEEELLPHRVSIDDDQIEEERRLAYVGITRAQKGLCFTLAKQRRRAGELQDCVPSRFLDELPQESLEWFGK
GGERCEEQSKKLAKSHLEGLKNLLS
>Mature_665_residues
MLNSQQMAAVKYIDGPLLVLAGAGSGKTRVITQKIGYLINSCGYNANSICAVTFTNKAANEMRARVSAFLPAASRRGLKV
ATFHTLGLSIIKRDVSRCDLKPGFSIFDSEDCLQILRGFLPVNKATDRDFILKVQQQISLWKNNLLNPELILQNPPETLL
CDEALTIYPRYQEALKAYNAVDFDDLIRLPVDLLNEHADVLEYWQNKIRHLLVDEYQDSNTSQYLLVKKLAGVRAHFTVV
GDDDQSIYAWRGAKPENLAQLQKDFPRLKIIKLEQNYRSTSRILHAANQLIAHNQHLFEKKLWSDLGHGELLRVVSCKDE
QDEAEHVIADLISHKLRNRTKYSDYAILYRGNHQARIFEKVLRHHGIPYHISGGQSWFAKLEVRDIFAYLKLLCNEADDA
AFLRAITTPKRGIGESSLDALGRYAQSRGISLYHGSDHLGLSEFVADKQRMIFHDFKLWMEDIKKRISSDSVLEHLRQMV
EDIGYEAYIYEQCDSPAKAQKKMDNVWELLEWVNRLLNKEPEQSLMDVVNKLILIDILEQSDESSSDVVQLMTLHASKGL
EFPFVYLVGMEEELLPHRVSIDDDQIEEERRLAYVGITRAQKGLCFTLAKQRRRAGELQDCVPSRFLDELPQESLEWFGK
GGERCEEQSKKLAKSHLEGLKNLLS

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=671, Percent_Identity=50.6706408345753, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=641, Percent_Identity=37.4414976599064, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1787196, Length=339, Percent_Identity=28.3185840707965, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=450, Percent_Identity=32.8888888888889, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324477, Length=666, Percent_Identity=24.1741741741742, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76045; Mature: 76045

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSQQMAAVKYIDGPLLVLAGAGSGKTRVITQKIGYLINSCGYNANSICAVTFTNKAAN
CCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHH
EMRARVSAFLPAASRRGLKVATFHTLGLSIIKRDVSRCDLKPGFSIFDSEDCLQILRGFL
HHHHHHHHHCCHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
PVNKATDRDFILKVQQQISLWKNNLLNPELILQNPPETLLCDEALTIYPRYQEALKAYNA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEECCCHHHCCHHHHHHHHHHCC
VDFDDLIRLPVDLLNEHADVLEYWQNKIRHLLVDEYQDSNTSQYLLVKKLAGVRAHFTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEE
GDDDQSIYAWRGAKPENLAQLQKDFPRLKIIKLEQNYRSTSRILHAANQLIAHNQHLFEK
ECCCCCEEEECCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
KLWSDLGHGELLRVVSCKDEQDEAEHVIADLISHKLRNRTKYSDYAILYRGNHQARIFEK
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
VLRHHGIPYHISGGQSWFAKLEVRDIFAYLKLLCNEADDAAFLRAITTPKRGIGESSLDA
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHH
LGRYAQSRGISLYHGSDHLGLSEFVADKQRMIFHDFKLWMEDIKKRISSDSVLEHLRQMV
HHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
EDIGYEAYIYEQCDSPAKAQKKMDNVWELLEWVNRLLNKEPEQSLMDVVNKLILIDILEQ
HHCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
SDESSSDVVQLMTLHASKGLEFPFVYLVGMEEELLPHRVSIDDDQIEEERRLAYVGITRA
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHEEEEEHHHH
QKGLCFTLAKQRRRAGELQDCVPSRFLDELPQESLEWFGKGGERCEEQSKKLAKSHLEGL
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KNLLS
HHHHC
>Mature Secondary Structure
MLNSQQMAAVKYIDGPLLVLAGAGSGKTRVITQKIGYLINSCGYNANSICAVTFTNKAAN
CCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHH
EMRARVSAFLPAASRRGLKVATFHTLGLSIIKRDVSRCDLKPGFSIFDSEDCLQILRGFL
HHHHHHHHHCCHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
PVNKATDRDFILKVQQQISLWKNNLLNPELILQNPPETLLCDEALTIYPRYQEALKAYNA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEECCCHHHCCHHHHHHHHHHCC
VDFDDLIRLPVDLLNEHADVLEYWQNKIRHLLVDEYQDSNTSQYLLVKKLAGVRAHFTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEE
GDDDQSIYAWRGAKPENLAQLQKDFPRLKIIKLEQNYRSTSRILHAANQLIAHNQHLFEK
ECCCCCEEEECCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
KLWSDLGHGELLRVVSCKDEQDEAEHVIADLISHKLRNRTKYSDYAILYRGNHQARIFEK
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
VLRHHGIPYHISGGQSWFAKLEVRDIFAYLKLLCNEADDAAFLRAITTPKRGIGESSLDA
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHH
LGRYAQSRGISLYHGSDHLGLSEFVADKQRMIFHDFKLWMEDIKKRISSDSVLEHLRQMV
HHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
EDIGYEAYIYEQCDSPAKAQKKMDNVWELLEWVNRLLNKEPEQSLMDVVNKLILIDILEQ
HHCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
SDESSSDVVQLMTLHASKGLEFPFVYLVGMEEELLPHRVSIDDDQIEEERRLAYVGITRA
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHEEEEEHHHH
QKGLCFTLAKQRRRAGELQDCVPSRFLDELPQESLEWFGKGGERCEEQSKKLAKSHLEGL
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KNLLS
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]