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The map label for this gene is nusA [H]

Identifier: 54295603

GI number: 54295603

Start: 3070053

End: 3071531

Strand: Reverse

Name: nusA [H]

Synonym: lpl2690

Alternate gene names: 54295603

Gene position: 3071531-3070053 (Counterclockwise)

Preceding gene: 54295604

Following gene: 54295602

Centisome position: 91.81

GC content: 41.04

Gene sequence:

>1479_bases
ATGAGCAAAGAATTGTTATTAGTTGCTGAGGCATTATCAAACGAAAGAGGCGTTAGTAAAGACGTCATTATACTTGCCAT
TCAGGCTGCACTGGAATCTGCAACTCGAAAGATTCTTGGTTTAGATATAGGTGTGCGTATCAAAATAGATTCTAAAACCG
GTGACTATGAAACCTTTAGATACTGGGATGTAGTGAATGAAGAGGAATTAGAGTTTCCGGACCGCCAACTGTCCTTGGAA
CAAGCAAAAGAGCGAAAGCCCTCGGCTGCTGTTGGTGACCGAATTGAAGAAATTATTCCTTCCATAGAATTTGGAAGAAT
TGAAGCGCAAACAGCGCGACAAGTTATTATGCAAAAAGTCAGGGAAGCGGAACGCGAATTGATCATAGACCAATTCCGTA
GCAAACTTGGGCAGCTAATCTATGGAACTGTTAAAAAAGTAACTCGTGACAATATAATTATTGATTTAGGTGGTAAAGCA
GAGGCTTTCTTGCCACGCTCTGAAATGTTGCCTCATGAAATGTTTCGTCCCAATGACAGAGTTCGAGCATATCTGTACGA
AATCACGCCGCAGGCGAGAGGACCACAATTGTTCGTAAGCAGAACTCGAAATGAAATGCTCATTGAATTATTCCGTATTG
AAGTTCCGGAAATTGGTGAAAATATAATAGATATCAAAGCTGCAGCGCGCGATCCGGGAAATAGAGCCAAAATTGCTGTT
AAAACCAATGATGGGCGTATTGACCCCATCGGCGCTTGCGTTGGTATGCGTGGAGCAAGGGTACAAGCGGTTTCCAGCGA
ATTAGGCGGTGAGCGAGTTGATATCATTCTATGGGATGATAACCCTGCCCAATTAGTCATAAATGCGATGGCTCCAGCTG
ATATTACATCTATCGTTGTGGATGAAGATACCCATACCATGGATTTAGCTGTTGTGAAAGAACAATTATCGCAGGCAATT
GGTCGAAACGGACAAAATGTTCGTTTGGCTAGCCAATTAACTGGTTGGACATTGAATGTCATGACAACGGAAGAGTTTGA
AAGCAAGAATCAAGAGGAATCAAGTAAAATAGTTAATCTGTTTACCAGCGCGCTAGAAATCGATGAGGAAATAGCGACAT
TATTGGTAGCTCATGGATTTTCTTCTCTCGAAGAAGTGGCTTATGTTCCGAAGGAGGAGCTGCTGGCTATTGAAGAATTT
GATGAAGAGATAGTTGAAGAGTTAAGAAACAGAGCAAATGACAAGTTATTGGAGATGGCACTGTCATCAGGCAAAGGATT
ATCTGGTACTCCAGATGATTCCCTTATCAATATGGAAGGCATGACAGAGGATTTGGCAAATAAATTAGCAAGTAAAGGAA
TTACAACAATGGAAGATCTTGCAGAGCAATCTGTAGATGAATTAATGGAAATAGAAGGCATTAGTGAAGAAAAAGCAGGT
GCTTTAATTATGAAAGCAAGAGAACCATGGTTTCAATAA

Upstream 100 bases:

>100_bases
CATCAGGAGATACAATAGTATTGGATATTAATAATGAACATCAAGAAATACTTTTATCAAATATTGTGAAAGCAAATTTG
ACAGTCTAGAGAGGCGAACA

Downstream 100 bases:

>100_bases
ACTATGGTTATCGAAATTGATCAATACGATGCTAGATGCATTTTAAATATATTCAGCTTTATAAAAAGTTGCTGAGAGGG
GATTAAATATGGCGGATGTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSKELLLVAEALSNERGVSKDVIILAIQAALESATRKILGLDIGVRIKIDSKTGDYETFRYWDVVNEEELEFPDRQLSLE
QAKERKPSAAVGDRIEEIIPSIEFGRIEAQTARQVIMQKVREAERELIIDQFRSKLGQLIYGTVKKVTRDNIIIDLGGKA
EAFLPRSEMLPHEMFRPNDRVRAYLYEITPQARGPQLFVSRTRNEMLIELFRIEVPEIGENIIDIKAAARDPGNRAKIAV
KTNDGRIDPIGACVGMRGARVQAVSSELGGERVDIILWDDNPAQLVINAMAPADITSIVVDEDTHTMDLAVVKEQLSQAI
GRNGQNVRLASQLTGWTLNVMTTEEFESKNQEESSKIVNLFTSALEIDEEIATLLVAHGFSSLEEVAYVPKEELLAIEEF
DEEIVEELRNRANDKLLEMALSSGKGLSGTPDDSLINMEGMTEDLANKLASKGITTMEDLAEQSVDELMEIEGISEEKAG
ALIMKAREPWFQ

Sequences:

>Translated_492_residues
MSKELLLVAEALSNERGVSKDVIILAIQAALESATRKILGLDIGVRIKIDSKTGDYETFRYWDVVNEEELEFPDRQLSLE
QAKERKPSAAVGDRIEEIIPSIEFGRIEAQTARQVIMQKVREAERELIIDQFRSKLGQLIYGTVKKVTRDNIIIDLGGKA
EAFLPRSEMLPHEMFRPNDRVRAYLYEITPQARGPQLFVSRTRNEMLIELFRIEVPEIGENIIDIKAAARDPGNRAKIAV
KTNDGRIDPIGACVGMRGARVQAVSSELGGERVDIILWDDNPAQLVINAMAPADITSIVVDEDTHTMDLAVVKEQLSQAI
GRNGQNVRLASQLTGWTLNVMTTEEFESKNQEESSKIVNLFTSALEIDEEIATLLVAHGFSSLEEVAYVPKEELLAIEEF
DEEIVEELRNRANDKLLEMALSSGKGLSGTPDDSLINMEGMTEDLANKLASKGITTMEDLAEQSVDELMEIEGISEEKAG
ALIMKAREPWFQ
>Mature_491_residues
SKELLLVAEALSNERGVSKDVIILAIQAALESATRKILGLDIGVRIKIDSKTGDYETFRYWDVVNEEELEFPDRQLSLEQ
AKERKPSAAVGDRIEEIIPSIEFGRIEAQTARQVIMQKVREAERELIIDQFRSKLGQLIYGTVKKVTRDNIIIDLGGKAE
AFLPRSEMLPHEMFRPNDRVRAYLYEITPQARGPQLFVSRTRNEMLIELFRIEVPEIGENIIDIKAAARDPGNRAKIAVK
TNDGRIDPIGACVGMRGARVQAVSSELGGERVDIILWDDNPAQLVINAMAPADITSIVVDEDTHTMDLAVVKEQLSQAIG
RNGQNVRLASQLTGWTLNVMTTEEFESKNQEESSKIVNLFTSALEIDEEIATLLVAHGFSSLEEVAYVPKEELLAIEEFD
EEIVEELRNRANDKLLEMALSSGKGLSGTPDDSLINMEGMTEDLANKLASKGITTMEDLAEQSVDELMEIEGISEEKAGA
LIMKAREPWFQ

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=493, Percent_Identity=60.0405679513185, Blast_Score=596, Evalue=1e-171,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54868; Mature: 54737

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKELLLVAEALSNERGVSKDVIILAIQAALESATRKILGLDIGVRIKIDSKTGDYETFR
CCCCCEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCCCCCCEE
YWDVVNEEELEFPDRQLSLEQAKERKPSAAVGDRIEEIIPSIEFGRIEAQTARQVIMQKV
EEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
REAERELIIDQFRSKLGQLIYGTVKKVTRDNIIIDLGGKAEAFLPRSEMLPHEMFRPNDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCHHHHCCCCH
VRAYLYEITPQARGPQLFVSRTRNEMLIELFRIEVPEIGENIIDIKAAARDPGNRAKIAV
HEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCCCEEEEE
KTNDGRIDPIGACVGMRGARVQAVSSELGGERVDIILWDDNPAQLVINAMAPADITSIVV
ECCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEEECCCCCEEEEEE
DEDTHTMDLAVVKEQLSQAIGRNGQNVRLASQLTGWTLNVMTTEEFESKNQEESSKIVNL
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECHHHHCCCHHHHHHHHHH
FTSALEIDEEIATLLVAHGFSSLEEVAYVPKEELLAIEEFDEEIVEELRNRANDKLLEMA
HHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGKGLSGTPDDSLINMEGMTEDLANKLASKGITTMEDLAEQSVDELMEIEGISEEKAG
HHCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
ALIMKAREPWFQ
EEEEECCCCCCC
>Mature Secondary Structure 
SKELLLVAEALSNERGVSKDVIILAIQAALESATRKILGLDIGVRIKIDSKTGDYETFR
CCCCEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCCCCCCEE
YWDVVNEEELEFPDRQLSLEQAKERKPSAAVGDRIEEIIPSIEFGRIEAQTARQVIMQKV
EEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
REAERELIIDQFRSKLGQLIYGTVKKVTRDNIIIDLGGKAEAFLPRSEMLPHEMFRPNDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCHHHHCCCCH
VRAYLYEITPQARGPQLFVSRTRNEMLIELFRIEVPEIGENIIDIKAAARDPGNRAKIAV
HEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCCCEEEEE
KTNDGRIDPIGACVGMRGARVQAVSSELGGERVDIILWDDNPAQLVINAMAPADITSIVV
ECCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEEECCCCCEEEEEE
DEDTHTMDLAVVKEQLSQAIGRNGQNVRLASQLTGWTLNVMTTEEFESKNQEESSKIVNL
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECHHHHCCCHHHHHHHHHH
FTSALEIDEEIATLLVAHGFSSLEEVAYVPKEELLAIEEFDEEIVEELRNRANDKLLEMA
HHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGKGLSGTPDDSLINMEGMTEDLANKLASKGITTMEDLAEQSVDELMEIEGISEEKAG
HHCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
ALIMKAREPWFQ
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]