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The map label for this gene is pnp [H]

Identifier: 54295598

GI number: 54295598

Start: 3063358

End: 3065547

Strand: Reverse

Name: pnp [H]

Synonym: lpl2685

Alternate gene names: 54295598

Gene position: 3065547-3063358 (Counterclockwise)

Preceding gene: 54295599

Following gene: 54295597

Centisome position: 91.63

GC content: 41.23

Gene sequence:

>2190_bases
GTGGCAAAAATTACAAAAGAAATAGTATTCGGTAACCATAAATTAATCTTGGAAACAGGTGAAGTAGCACGACAGGCTGA
TGGTGCCGTAATGGCAAGTATGAACGGTACACAAGTGCTAGTGACTGTAGTTTGGAAAAAGGATGGTGGTGAAAGTAATG
ATTTTTTCCCATTAACAGTGAATTATCAAGAAAAGTTCTATGCCATAGGTAAAATCCCAGGTGGCTTTAACAAACGTGAA
GGACGGCCATCTGATAATGAAACATTAATTTCTCGGTTAATAGATAGACCGATCCGGCCATTATTCCCGGATAATTTTTT
TAACGAAGTACAGATAATTGCTACTGTCCTGTCCTTAAATCCTGAGGTATCTCCTGATATTATCGCTATGATTGGAGCCT
CCGCAGCGCTTTCAATTTCTGGCGTGCCATTTAATGGCCCTATAGGTGCCGCTCGAGTAGGCTATAAAGATGGCGTTTAT
CTGCTCAATCCAAGTAGAAAAGAACAAGAAGAGTCCAAGCTTGATTTGGTTATTGCGGGAACAAAAGATGCCATTTTGAT
GGTGGAATCAGAAGCACAGGAATTAAGTGAAGACATCATGCGTGGTGCTATGTTATATGGCCATGAAATGATGAAAAATG
TCATAAAATCAATAGAAGAACTTGCTAGAGACGTGGGTAAGAGCAAACCTGAGTGGAAAGCACCAGAAATAGATACAGTA
CTAAAAGCTAGAATCAATGACGTAGCTAGGAATGAAGTTGAAGCCGCTTATCTTATTAAAGACAAGCAACAACGGTATCA
GCGATTAGGCGAATTAAGAGAGCAAACTATTTCTGCTTTATTGGCTGAAAATGATGAGTTAAATGCAGATGTTATTGCCA
ATATGTTTGGTGAGCTAGAACGTTCTATAGTACGTAATCGTATTCTTGACGGCGAGCCTCGCATCGATGGTCGTGATCAC
AGAACAGTTAGGCCTATTTCTGTTCGTACCAAATTCTTGGAAAGAACTCATGGTTCCTGTTTGTTTACCAGAGGAGAAAC
TCAAGCTATTGTTGTTGCCACTTTGGGTAATGAACGTGATGCACAGATATTAGACGGAATTAGTGGCGAAAGCAGAGATC
GATTTATGCTTCATTATAATTTTCCTCCTTATTCTGTCGGTGAAACAGGGCAAGTGGGCAGTCCTAAACGGCGTGAAATT
GGGCATGGCCGTTTAGCTAAGCGTGCTTTAATGGCTGTACTGCCTGATGCTAATGAGTTTCCTTATGTACTACGTATTGT
ATCTGAGATTACCGAATCCAATGGCTCCAGTTCTATGGCTACTGTCTGTGGAACAAGTCTGGCATTGATGGATGCCGGGG
TACCTTTAAAAGCACCAGTCGCTGGTGTGGCCATGGGCTTAATCAAGGAAGGTGATCGTTATGCTGTATTAACAGACATA
TTGGGTGATGAGGATCATTTGGGTGATATGGACTTTAAAGTGGCCGGTACGGAAAAAGGGATTACTGCTTTGCAGATGGA
TATCAAAATTTCCGGGATTACCAATGAAATTATGGAACGGGCTCTGGAGCAAGCATTGGAAGGTCGTACCCATATTCTTG
GTGTTATGAATAATGCTCTTGCCGAACATAGAACCGAGTTATCTCAGCACGCTCCAAGAATCACAACCATGAAAGTCGCT
GAAGATAAAATCCGCACAATTATTGGCAAAGGCGGTGCAACAATCAAAGGACTTATTGAAAGTACCGGCGTATCTATAGA
TATTGATGACTCAGGGGTTATCCAATTATTTTCTCCGGATAAGATTGCTTTGGAAGAGGCGCAAAAACAAATTAAAGCTT
TAATTGCTGAAATTGAAGTAGGTCAGACTTATCAAGGGAAAGTAAGCAAGATAGTTGACTTTGGAGCCTTTATCAATTTA
CTGCCTGGTAAAGATGGCTTACTGCATATTTCACAAATATGTGCCGCCAGAACACAAAAAGTAGAAGAGGTATTGCAGGA
AGGTCAGGAAATTGAAGTCTTTGTTGCTGGGATAGATAAGCAAGGACGAGTCAAACTGGAGTGGAAGGATAAGCCACAAG
CTGAAGCCAAAGAGGTCGAAGACGCCCCGGTATCTGCTACGTTTCTTACAATGGAAGAGCAATCTGAAGAAATTAATTCA
GGCAACAAAATCTCTGAAGAAGAGGAATAA

Upstream 100 bases:

>100_bases
GTCTCTATGGGGACGCATCAAATAATAAATCAGTTTTAGAATAAAAGGCGCAGCGTTCTGCGCCTTTTTTTTCTTATATT
TACTTAATGGGGAACATTAC

Downstream 100 bases:

>100_bases
TAAGATAGTAGCATTTCAAGTTCTATTTATTATAAACTCGTGGAAGGTATGAGCTTTTCGCGAGTTTTAACTGAGTGCTT
GCAATTGGTATATATGACCG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MAKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVVWKKDGGESNDFFPLTVNYQEKFYAIGKIPGGFNKRE
GRPSDNETLISRLIDRPIRPLFPDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVY
LLNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSIEELARDVGKSKPEWKAPEIDTV
LKARINDVARNEVEAAYLIKDKQQRYQRLGELREQTISALLAENDELNADVIANMFGELERSIVRNRILDGEPRIDGRDH
RTVRPISVRTKFLERTHGSCLFTRGETQAIVVATLGNERDAQILDGISGESRDRFMLHYNFPPYSVGETGQVGSPKRREI
GHGRLAKRALMAVLPDANEFPYVLRIVSEITESNGSSSMATVCGTSLALMDAGVPLKAPVAGVAMGLIKEGDRYAVLTDI
LGDEDHLGDMDFKVAGTEKGITALQMDIKISGITNEIMERALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVA
EDKIRTIIGKGGATIKGLIESTGVSIDIDDSGVIQLFSPDKIALEEAQKQIKALIAEIEVGQTYQGKVSKIVDFGAFINL
LPGKDGLLHISQICAARTQKVEEVLQEGQEIEVFVAGIDKQGRVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINS
GNKISEEEE

Sequences:

>Translated_729_residues
MAKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVVWKKDGGESNDFFPLTVNYQEKFYAIGKIPGGFNKRE
GRPSDNETLISRLIDRPIRPLFPDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVY
LLNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSIEELARDVGKSKPEWKAPEIDTV
LKARINDVARNEVEAAYLIKDKQQRYQRLGELREQTISALLAENDELNADVIANMFGELERSIVRNRILDGEPRIDGRDH
RTVRPISVRTKFLERTHGSCLFTRGETQAIVVATLGNERDAQILDGISGESRDRFMLHYNFPPYSVGETGQVGSPKRREI
GHGRLAKRALMAVLPDANEFPYVLRIVSEITESNGSSSMATVCGTSLALMDAGVPLKAPVAGVAMGLIKEGDRYAVLTDI
LGDEDHLGDMDFKVAGTEKGITALQMDIKISGITNEIMERALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVA
EDKIRTIIGKGGATIKGLIESTGVSIDIDDSGVIQLFSPDKIALEEAQKQIKALIAEIEVGQTYQGKVSKIVDFGAFINL
LPGKDGLLHISQICAARTQKVEEVLQEGQEIEVFVAGIDKQGRVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINS
GNKISEEEE
>Mature_728_residues
AKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVVWKKDGGESNDFFPLTVNYQEKFYAIGKIPGGFNKREG
RPSDNETLISRLIDRPIRPLFPDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVYL
LNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSIEELARDVGKSKPEWKAPEIDTVL
KARINDVARNEVEAAYLIKDKQQRYQRLGELREQTISALLAENDELNADVIANMFGELERSIVRNRILDGEPRIDGRDHR
TVRPISVRTKFLERTHGSCLFTRGETQAIVVATLGNERDAQILDGISGESRDRFMLHYNFPPYSVGETGQVGSPKRREIG
HGRLAKRALMAVLPDANEFPYVLRIVSEITESNGSSSMATVCGTSLALMDAGVPLKAPVAGVAMGLIKEGDRYAVLTDIL
GDEDHLGDMDFKVAGTEKGITALQMDIKISGITNEIMERALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVAE
DKIRTIIGKGGATIKGLIESTGVSIDIDDSGVIQLFSPDKIALEEAQKQIKALIAEIEVGQTYQGKVSKIVDFGAFINLL
PGKDGLLHISQICAARTQKVEEVLQEGQEIEVFVAGIDKQGRVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINSG
NKISEEEE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=747, Percent_Identity=36.813922356091, Blast_Score=446, Evalue=1e-125,
Organism=Escherichia coli, GI145693187, Length=687, Percent_Identity=59.5342066957787, Blast_Score=852, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=654, Percent_Identity=35.0152905198777, Blast_Score=344, Evalue=9e-95,
Organism=Drosophila melanogaster, GI281362905, Length=710, Percent_Identity=37.6056338028169, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651641, Length=710, Percent_Identity=37.6056338028169, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651643, Length=710, Percent_Identity=37.6056338028169, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI161079377, Length=637, Percent_Identity=37.6766091051805, Blast_Score=421, Evalue=1e-118,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 80016; Mature: 79885

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVVWKKDGGESNDFFPLTV
CCCCCHHHHCCCCEEEEECCHHHHHCCCCEEEECCCCEEEEEEEEECCCCCCCCEEEEEE
NYQEKFYAIGKIPGGFNKREGRPSDNETLISRLIDRPIRPLFPDNFFNEVQIIATVLSLN
ECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
PEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVYLLNPSRKEQEESKLDLVIAG
CCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHCCEEEEEEC
TKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSIEELARDVGKSKPEWKAPEIDTV
CCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LKARINDVARNEVEAAYLIKDKQQRYQRLGELREQTISALLAENDELNADVIANMFGELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RSIVRNRILDGEPRIDGRDHRTVRPISVRTKFLERTHGSCLFTRGETQAIVVATLGNERD
HHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCC
AQILDGISGESRDRFMLHYNFPPYSVGETGQVGSPKRREIGHGRLAKRALMAVLPDANEF
HHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCC
PYVLRIVSEITESNGSSSMATVCGTSLALMDAGVPLKAPVAGVAMGLIKEGDRYAVLTDI
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEC
LGDEDHLGDMDFKVAGTEKGITALQMDIKISGITNEIMERALEQALEGRTHILGVMNNAL
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AEHRTELSQHAPRITTMKVAEDKIRTIIGKGGATIKGLIESTGVSIDIDDSGVIQLFSPD
HHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCEEEEECCC
KIALEEAQKQIKALIAEIEVGQTYQGKVSKIVDFGAFINLLPGKDGLLHISQICAARTQK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHH
VEEVLQEGQEIEVFVAGIDKQGRVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINS
HHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEHHHHHHHCC
GNKISEEEE
CCCCCCCCC
>Mature Secondary Structure 
AKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVVWKKDGGESNDFFPLTV
CCCCHHHHCCCCEEEEECCHHHHHCCCCEEEECCCCEEEEEEEEECCCCCCCCEEEEEE
NYQEKFYAIGKIPGGFNKREGRPSDNETLISRLIDRPIRPLFPDNFFNEVQIIATVLSLN
ECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
PEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVYLLNPSRKEQEESKLDLVIAG
CCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHCCEEEEEEC
TKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSIEELARDVGKSKPEWKAPEIDTV
CCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LKARINDVARNEVEAAYLIKDKQQRYQRLGELREQTISALLAENDELNADVIANMFGELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RSIVRNRILDGEPRIDGRDHRTVRPISVRTKFLERTHGSCLFTRGETQAIVVATLGNERD
HHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCC
AQILDGISGESRDRFMLHYNFPPYSVGETGQVGSPKRREIGHGRLAKRALMAVLPDANEF
HHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCC
PYVLRIVSEITESNGSSSMATVCGTSLALMDAGVPLKAPVAGVAMGLIKEGDRYAVLTDI
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEC
LGDEDHLGDMDFKVAGTEKGITALQMDIKISGITNEIMERALEQALEGRTHILGVMNNAL
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AEHRTELSQHAPRITTMKVAEDKIRTIIGKGGATIKGLIESTGVSIDIDDSGVIQLFSPD
HHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCEEEEECCC
KIALEEAQKQIKALIAEIEVGQTYQGKVSKIVDFGAFINLLPGKDGLLHISQICAARTQK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHH
VEEVLQEGQEIEVFVAGIDKQGRVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINS
HHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEHHHHHHHCC
GNKISEEEE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA