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The map label for this gene is cca [H]

Identifier: 54295580

GI number: 54295580

Start: 3037406

End: 3038638

Strand: Reverse

Name: cca [H]

Synonym: lpl2667

Alternate gene names: 54295580

Gene position: 3038638-3037406 (Counterclockwise)

Preceding gene: 54295582

Following gene: 54295579

Centisome position: 90.82

GC content: 41.12

Gene sequence:

>1233_bases
ATGAAAGTCTACTTGGTAGGCGGTGCAGTTCGTGATCGATTATTAGGAATTCCTGTCCAGGAACAGGATTGGGTAGTCGT
CGGAGCAACGCCGGAAGAATTATTAAAAAGAAAATATAGGCAAGTTGGGCGGGATTTCCCCGTTTTTTTGCATCCTGAAA
CGAAAGAGGAATATGCATTAGCAAGAACAGAGCGTAAATCAGCTCCAGGATATTATGGATTCATTTGTGATTTTAGTGAA
TCAGTGACGTTGGAAGAGGATTTGGCGCGACGAGATTTAACGATTAATGCCATGGCTATGGATGAACAGGGAAATTTAAT
TGATCCATATCAGGGACAGCGAGATCTCGAAGAGAAATTATTACGTCATGTGTCTTCCGCTTTTGCAGAAGATCCTGTTC
GGGTTTTACGTGTTGCTCGTTTTGCCAGTCGGTTTCATCATCTTGGATTTAGAATAGCCAATGAGACTCGTTTATTAATG
TATTCGATGGTTAAACAAGGTGAATTAGCTCATTTGATCCCCGAGCGTGTTTGGCAGGAATGGCAAAAAAGTCTTGAAGA
AAAAAATCCTGAACAATTCATTCTTTCTTTACGTTCCTGTGATGCATTAAGAGTGATTTTGCCAGAAATTAACTCATTAT
TTGGTGTGCCTAATCCACATCAATACCATCAAGAAATAGATACGGGCATTCATTCATTAATGACATTACGGGCATCATCT
GAGCTTAGCGAAGAACCATTAGTACGATTTGCTGCTTTGGTTCATGATTTAGGCAAAGCCTCGACACCAATTCAGGCATG
GCCTAAACACCATGGGCATGAGGAGGAGGGTACCAAGCTGATTCGAGCATTATGTGCTCGCTTGCGCATTCCTAATGATT
ATCGCGACTTGGCCGTTACAGTGGCGCGAGCTCATTTGAATATTCATAGGGTATGCGAATTGCGTCCTAACACAATCGTC
AAGCTTTTGGAGCAGGTAGATGCCTTTAGACGACCTCAATTATTTCATAAAATATTGATTGCTTGTCAAGCAGATGCCGA
GAGTTGTGGCAAAACAGTGGTCTATCGCCAAACTCAATTATGGAATGAAATATTATCTGAATGTGTTAAAGTGACGCCAC
AAACTTTTATCGTACAAGGTTATGAAGGTAAAGCGATTAAGGAGGCCATGCATCAAAGTCGTGTGGCTTGTGTAGAGCGA
ATTATGACTTCTTGGAAATCAAATGAAAAATAA

Upstream 100 bases:

>100_bases
CGGGCAGATTGCTGCTTATTGATTCGTCTTTTACTCATAGTGTCAATCTATACTATAATTGATGCGATTAATAATACAGT
ATGAAGGTAAATCATATTTA

Downstream 100 bases:

>100_bases
TCAAAATTTAATTTGGATTGATTTGGAAATGACTGGCCTTGAGCCAGAACAAGATAGAATTATAGAAATGGCCACGATTG
TAACGGATCCACAACTTAAC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRKYRQVGRDFPVFLHPETKEEYALARTERKSAPGYYGFICDFSE
SVTLEEDLARRDLTINAMAMDEQGNLIDPYQGQRDLEEKLLRHVSSAFAEDPVRVLRVARFASRFHHLGFRIANETRLLM
YSMVKQGELAHLIPERVWQEWQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPNPHQYHQEIDTGIHSLMTLRASS
ELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEEGTKLIRALCARLRIPNDYRDLAVTVARAHLNIHRVCELRPNTIV
KLLEQVDAFRRPQLFHKILIACQADAESCGKTVVYRQTQLWNEILSECVKVTPQTFIVQGYEGKAIKEAMHQSRVACVER
IMTSWKSNEK

Sequences:

>Translated_410_residues
MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRKYRQVGRDFPVFLHPETKEEYALARTERKSAPGYYGFICDFSE
SVTLEEDLARRDLTINAMAMDEQGNLIDPYQGQRDLEEKLLRHVSSAFAEDPVRVLRVARFASRFHHLGFRIANETRLLM
YSMVKQGELAHLIPERVWQEWQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPNPHQYHQEIDTGIHSLMTLRASS
ELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEEGTKLIRALCARLRIPNDYRDLAVTVARAHLNIHRVCELRPNTIV
KLLEQVDAFRRPQLFHKILIACQADAESCGKTVVYRQTQLWNEILSECVKVTPQTFIVQGYEGKAIKEAMHQSRVACVER
IMTSWKSNEK
>Mature_410_residues
MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRKYRQVGRDFPVFLHPETKEEYALARTERKSAPGYYGFICDFSE
SVTLEEDLARRDLTINAMAMDEQGNLIDPYQGQRDLEEKLLRHVSSAFAEDPVRVLRVARFASRFHHLGFRIANETRLLM
YSMVKQGELAHLIPERVWQEWQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPNPHQYHQEIDTGIHSLMTLRASS
ELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEEGTKLIRALCARLRIPNDYRDLAVTVARAHLNIHRVCELRPNTIV
KLLEQVDAFRRPQLFHKILIACQADAESCGKTVVYRQTQLWNEILSECVKVTPQTFIVQGYEGKAIKEAMHQSRVACVER
IMTSWKSNEK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=408, Percent_Identity=54.9019607843137, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI87081691, Length=171, Percent_Identity=35.0877192982456, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281360112, Length=274, Percent_Identity=26.6423357664234, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21357337, Length=274, Percent_Identity=26.6423357664234, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47135; Mature: 47135

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRKYRQVGRDFPVFLHPETKEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKSAPGYYGFICDFSESVTLEEDLARRDLTINAMAMDEQGNLIDPYQGQRDLEEKL
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
LRHVSSAFAEDPVRVLRVARFASRFHHLGFRIANETRLLMYSMVKQGELAHLIPERVWQE
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCHHHHHHHHHHHH
WQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPNPHQYHQEIDTGIHSLMTLRASS
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEEGTKLIRALCARLRIPNDYRDLAVT
CCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
VARAHLNIHRVCELRPNTIVKLLEQVDAFRRPQLFHKILIACQADAESCGKTVVYRQTQL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
WNEILSECVKVTPQTFIVQGYEGKAIKEAMHQSRVACVERIMTSWKSNEK
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRKYRQVGRDFPVFLHPETKEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKSAPGYYGFICDFSESVTLEEDLARRDLTINAMAMDEQGNLIDPYQGQRDLEEKL
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
LRHVSSAFAEDPVRVLRVARFASRFHHLGFRIANETRLLMYSMVKQGELAHLIPERVWQE
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCHHHHHHHHHHHH
WQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPNPHQYHQEIDTGIHSLMTLRASS
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEEGTKLIRALCARLRIPNDYRDLAVT
CCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
VARAHLNIHRVCELRPNTIVKLLEQVDAFRRPQLFHKILIACQADAESCGKTVVYRQTQL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
WNEILSECVKVTPQTFIVQGYEGKAIKEAMHQSRVACVERIMTSWKSNEK
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA