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The map label for this gene is tphB [H]

Identifier: 54294983

GI number: 54294983

Start: 2320595

End: 2321863

Strand: Direct

Name: tphB [H]

Synonym: lpl2062

Alternate gene names: 54294983

Gene position: 2320595-2321863 (Clockwise)

Preceding gene: 54294981

Following gene: 54294988

Centisome position: 69.36

GC content: 37.59

Gene sequence:

>1269_bases
TTGATTTATTGTATGGAAAATAAGAAGAAAAGGGTCGTTGTTGCAACCGCCGGAACTTTTCTGGAATGGGCTGAGTTTAC
TTATTACGCCTATATCGCAAATATTATCTCGGGATTGTTTTTTCCAAAATTGGGAAATCATCTTGGTTTGATAGCAACCT
TTTCTGTTTTCGCTCTCAGTTATTTTTTTCGTCCATTAGGAGCCCTCTTTTTTGGATATATTGGAGACAAGATGGGGCGT
CGAGTTGCCTTGCAATCTTCTATTATGCTGATGGGCCTATCTTCTCTGTTGATTGGTTGTCTTCCTTCTTACAACAGTAT
CGGTATCTTGGCTCCAATTCTCTTGCTCATCTTTCGTTGCATACAAGGCTTTGCCGTATCAGGTGAATTTAATGGCAGCG
CGATCTATCTGATTGAACATGATTCACAAAAACCATGCCAGGCTGGTAGCTGGACTGGATTTGCTTCCGCATTGGGTATG
ATGTTTGGCGGATTGATGTCTACTTTAATTTATCTACCTCACATGCCTGAGTGGGCATGGCGTATCCCTTTCCTGTTGGG
AACTTTATCCTGCTTTTGTTCGATGTATTTTCGTAAAAATTTGAGCGAATCACCGCCCTACCTCACACTAAGTAAAAAAA
AATCATCCAATCCACTGAAAATTTTATTTGAAAATTATAAAAAACAATTAATTAGGGCAGTATTATTAATAGCTGCATTA
GGCATTTATATCTACATTATGAATGTTTATTATGCTTCTCATTTGGCAAAATACACTACGTTGTCAGAAGCCCAAACCAA
ATTCGTTGTTACCTTAGGACAAGGATTGGTTGTACTATTCATTGTTTTAACAGGAATGTATGCTGATCGAATTGACGAAA
GACATATTATAAAAACCGGTTTGTGGGGGTATTTTATTGCGGCTCCTGTTGCTTTCCTGATCCCTCAAACCAATTCTTTT
GCATTAATTCTCATATCGCAGATTCCCTATGCATTATGCAATGCTCTGGTTGGCACACCAATTTTTAAACTATTGAATGA
TTTTTTTCCTACTCAGATTCGATATAGCGGTGTTTCTGTTGCATGGGGAGTAAGCATGGCTATTTTTGGAGGAACAGCGC
CATTGGTTGCCAATTATTTACAATATTCATTCAAATTAACAACTGCGCCAATATTCTATATACTTTTATCTGCCAGTATT
GCCTTGCTCGTTTTGCATGATAAAAAGAGTTACAAGATAACCTCCATAAAATTAAGATCAGATAATTGA

Upstream 100 bases:

>100_bases
TGACACTATAGTAACTCTATGAATCTGTTATATCATTCAGTTACATAGAATAATGAGAGCAATTTAAGGTAAAATCTCTC
TACATCACTTTAATATTCTA

Downstream 100 bases:

>100_bases
ATAATTGAATAAACGACTTATCACCATTGCAAAAACATGTCTACGCAAGCATCTAATCAATTTGTAACAATTGCTTCTTA
ATAGCCCCCATTGTCAGAGT

Product: proline/glycine betaine transporter-like protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MIYCMENKKKRVVVATAGTFLEWAEFTYYAYIANIISGLFFPKLGNHLGLIATFSVFALSYFFRPLGALFFGYIGDKMGR
RVALQSSIMLMGLSSLLIGCLPSYNSIGILAPILLLIFRCIQGFAVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGM
MFGGLMSTLIYLPHMPEWAWRIPFLLGTLSCFCSMYFRKNLSESPPYLTLSKKKSSNPLKILFENYKKQLIRAVLLIAAL
GIYIYIMNVYYASHLAKYTTLSEAQTKFVVTLGQGLVVLFIVLTGMYADRIDERHIIKTGLWGYFIAAPVAFLIPQTNSF
ALILISQIPYALCNALVGTPIFKLLNDFFPTQIRYSGVSVAWGVSMAIFGGTAPLVANYLQYSFKLTTAPIFYILLSASI
ALLVLHDKKSYKITSIKLRSDN

Sequences:

>Translated_422_residues
MIYCMENKKKRVVVATAGTFLEWAEFTYYAYIANIISGLFFPKLGNHLGLIATFSVFALSYFFRPLGALFFGYIGDKMGR
RVALQSSIMLMGLSSLLIGCLPSYNSIGILAPILLLIFRCIQGFAVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGM
MFGGLMSTLIYLPHMPEWAWRIPFLLGTLSCFCSMYFRKNLSESPPYLTLSKKKSSNPLKILFENYKKQLIRAVLLIAAL
GIYIYIMNVYYASHLAKYTTLSEAQTKFVVTLGQGLVVLFIVLTGMYADRIDERHIIKTGLWGYFIAAPVAFLIPQTNSF
ALILISQIPYALCNALVGTPIFKLLNDFFPTQIRYSGVSVAWGVSMAIFGGTAPLVANYLQYSFKLTTAPIFYILLSASI
ALLVLHDKKSYKITSIKLRSDN
>Mature_422_residues
MIYCMENKKKRVVVATAGTFLEWAEFTYYAYIANIISGLFFPKLGNHLGLIATFSVFALSYFFRPLGALFFGYIGDKMGR
RVALQSSIMLMGLSSLLIGCLPSYNSIGILAPILLLIFRCIQGFAVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGM
MFGGLMSTLIYLPHMPEWAWRIPFLLGTLSCFCSMYFRKNLSESPPYLTLSKKKSSNPLKILFENYKKQLIRAVLLIAAL
GIYIYIMNVYYASHLAKYTTLSEAQTKFVVTLGQGLVVLFIVLTGMYADRIDERHIIKTGLWGYFIAAPVAFLIPQTNSF
ALILISQIPYALCNALVGTPIFKLLNDFFPTQIRYSGVSVAWGVSMAIFGGTAPLVANYLQYSFKLTTAPIFYILLSASI
ALLVLHDKKSYKITSIKLRSDN

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=413, Percent_Identity=30.0242130750605, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788292, Length=423, Percent_Identity=27.1867612293144, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788942, Length=423, Percent_Identity=29.3144208037825, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=29.3706293706294, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87082231, Length=207, Percent_Identity=26.5700483091787, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082404, Length=366, Percent_Identity=24.5901639344262, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46997; Mature: 46997

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYCMENKKKRVVVATAGTFLEWAEFTYYAYIANIISGLFFPKLGNHLGLIATFSVFALS
CEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YFFRPLGALFFGYIGDKMGRRVALQSSIMLMGLSSLLIGCLPSYNSIGILAPILLLIFRC
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IQGFAVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGMMFGGLMSTLIYLPHMPEWAW
HCCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RIPFLLGTLSCFCSMYFRKNLSESPPYLTLSKKKSSNPLKILFENYKKQLIRAVLLIAAL
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GIYIYIMNVYYASHLAKYTTLSEAQTKFVVTLGQGLVVLFIVLTGMYADRIDERHIIKTG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHH
LWGYFIAAPVAFLIPQTNSFALILISQIPYALCNALVGTPIFKLLNDFFPTQIRYSGVSV
HHHHHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEE
AWGVSMAIFGGTAPLVANYLQYSFKLTTAPIFYILLSASIALLVLHDKKSYKITSIKLRS
EHHHHHHEECCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEEEC
DN
CC
>Mature Secondary Structure
MIYCMENKKKRVVVATAGTFLEWAEFTYYAYIANIISGLFFPKLGNHLGLIATFSVFALS
CEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YFFRPLGALFFGYIGDKMGRRVALQSSIMLMGLSSLLIGCLPSYNSIGILAPILLLIFRC
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IQGFAVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGMMFGGLMSTLIYLPHMPEWAW
HCCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RIPFLLGTLSCFCSMYFRKNLSESPPYLTLSKKKSSNPLKILFENYKKQLIRAVLLIAAL
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GIYIYIMNVYYASHLAKYTTLSEAQTKFVVTLGQGLVVLFIVLTGMYADRIDERHIIKTG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHH
LWGYFIAAPVAFLIPQTNSFALILISQIPYALCNALVGTPIFKLLNDFFPTQIRYSGVSV
HHHHHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEE
AWGVSMAIFGGTAPLVANYLQYSFKLTTAPIFYILLSASIALLVLHDKKSYKITSIKLRS
EHHHHHHEECCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEEEC
DN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA