Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 54294936

GI number: 54294936

Start: 2260302

End: 2261570

Strand: Direct

Name: eno [H]

Synonym: lpl2015

Alternate gene names: 54294936

Gene position: 2260302-2261570 (Clockwise)

Preceding gene: 54294935

Following gene: 54294937

Centisome position: 67.56

GC content: 39.8

Gene sequence:

>1269_bases
ATGCACATACATAAAATTCAGGCACGTGAAATATTAGATTCTCGCGGTAATCCGACTATTGAGGCAGACGTCATTCTAGA
AAACGGTATTATTGGAAGAGCCAGCGTTCCTTCAGGTGCTTCCACAGGAAGCCGGGAAGCCTGTGAACTTAGAGACAATG
ATCCAAAGCGATATGCTGGCAAAGGGGTGCAAAAAGCGGTTAAACACGTTAATGAAGAGATAAATCAGGCATTACAAGGA
TTGTCTGTTAGTGATCAGGAAAATTTAGATCGCATTTTATGCCAATTAGATAATACAGAAAACAAATCTCATCTGGGAGC
TAATGCTATCCTGGCCACTTCTCTGGCATGTGCCAGAGCAAGAGCATTATCTCTTAATCAGCCATTGTATATGACCCTTA
ATCAGGGTGACATGATGACCATGCCTGTTCCTATGATGAATATTCTGAATGGAGGCGCTCATGCTGATAATAATGTGGAT
ATTCAAGAATTTATGATCATGCCAATCGGCGCTCCTGATTTTCCAGTTGCATTACAAATGGGAACTGAAATTTTTCATGT
CCTGAAATCTGTATTAAAAAAGCAGGGATTAAATACTGCTGTTGGTGATGAAGGCGGTTTTGCACCTAATATTCAATCTA
ATCGTCAAGCGCTGGATCTTCTTAGCGAGGCGATTGAAAAGGCAGGCTTTCGATTAGGAGAAGACATTGTATTTGCCTTG
GATGTTGCTGCTTCTGAGCTTTTTAATGAAGGCTTTTATCATATGTCTTCTGAAAATCAAAAATTTGATTCTCATCAACT
TATAGAATACTACGCAAATCTCATTTCCAGTTATCCCATAGCCAGTATTGAAGATGGCCTGGATGAAAAAGACTGGTCAG
GATGGAAACAATTAACCACCCTTTTGGGAAATAAAGTCCAGCTCGTCGGAGATGATTTATTTGTAACTAATCCCAAAATT
CTGCAAGAAGGAATTGCTCAGGGAATTGCTAATGCCATATTAATTAAAGTCAATCAAATAGGTACTTTAAGCGAAACCAG
GCAAGCTATAAAACTCGCATACGACAATGGATACCGATGTGTCATGTCCCATCGTTCAGGAGAAACAGAAGATACCTTCA
TTGCTGATCTTGCAGTGGCCAGTGGTTGTGGCCAAATAAAAACCGGATCTTTGTGTCGAACAGACAGGACTGCGAAATAT
AATCAACTACTAAGAATCAATGAATTGGCGTCTTTACCCTATGCTGGAAAAAATATTCTAAAGAGATGA

Upstream 100 bases:

>100_bases
ACTATTCTTGGTTTGCCACTGATGCCATTAGTTAATGCGCTTGTAAACCTTAAGGTGGTAGGTATTTAAGTTAATAAATA
TAAAAAGATTAGGAAAGATC

Downstream 100 bases:

>100_bases
GTTCAAGCTTTCAGCATTATTAATGTTTAAGTTGGAGTGATGTTGAGTGCTGAATAAAACTCGGCATCAGCTCTAATTTA
AATCAAAGGTAATATAATTA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MHIHKIQAREILDSRGNPTIEADVILENGIIGRASVPSGASTGSREACELRDNDPKRYAGKGVQKAVKHVNEEINQALQG
LSVSDQENLDRILCQLDNTENKSHLGANAILATSLACARARALSLNQPLYMTLNQGDMMTMPVPMMNILNGGAHADNNVD
IQEFMIMPIGAPDFPVALQMGTEIFHVLKSVLKKQGLNTAVGDEGGFAPNIQSNRQALDLLSEAIEKAGFRLGEDIVFAL
DVAASELFNEGFYHMSSENQKFDSHQLIEYYANLISSYPIASIEDGLDEKDWSGWKQLTTLLGNKVQLVGDDLFVTNPKI
LQEGIAQGIANAILIKVNQIGTLSETRQAIKLAYDNGYRCVMSHRSGETEDTFIADLAVASGCGQIKTGSLCRTDRTAKY
NQLLRINELASLPYAGKNILKR

Sequences:

>Translated_422_residues
MHIHKIQAREILDSRGNPTIEADVILENGIIGRASVPSGASTGSREACELRDNDPKRYAGKGVQKAVKHVNEEINQALQG
LSVSDQENLDRILCQLDNTENKSHLGANAILATSLACARARALSLNQPLYMTLNQGDMMTMPVPMMNILNGGAHADNNVD
IQEFMIMPIGAPDFPVALQMGTEIFHVLKSVLKKQGLNTAVGDEGGFAPNIQSNRQALDLLSEAIEKAGFRLGEDIVFAL
DVAASELFNEGFYHMSSENQKFDSHQLIEYYANLISSYPIASIEDGLDEKDWSGWKQLTTLLGNKVQLVGDDLFVTNPKI
LQEGIAQGIANAILIKVNQIGTLSETRQAIKLAYDNGYRCVMSHRSGETEDTFIADLAVASGCGQIKTGSLCRTDRTAKY
NQLLRINELASLPYAGKNILKR
>Mature_422_residues
MHIHKIQAREILDSRGNPTIEADVILENGIIGRASVPSGASTGSREACELRDNDPKRYAGKGVQKAVKHVNEEINQALQG
LSVSDQENLDRILCQLDNTENKSHLGANAILATSLACARARALSLNQPLYMTLNQGDMMTMPVPMMNILNGGAHADNNVD
IQEFMIMPIGAPDFPVALQMGTEIFHVLKSVLKKQGLNTAVGDEGGFAPNIQSNRQALDLLSEAIEKAGFRLGEDIVFAL
DVAASELFNEGFYHMSSENQKFDSHQLIEYYANLISSYPIASIEDGLDEKDWSGWKQLTTLLGNKVQLVGDDLFVTNPKI
LQEGIAQGIANAILIKVNQIGTLSETRQAIKLAYDNGYRCVMSHRSGETEDTFIADLAVASGCGQIKTGSLCRTDRTAKY
NQLLRINELASLPYAGKNILKR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=420, Percent_Identity=51.9047619047619, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=433, Percent_Identity=50.1154734411085, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=420, Percent_Identity=50.4761904761905, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI301897469, Length=420, Percent_Identity=50.4761904761905, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI301897479, Length=418, Percent_Identity=45.4545454545455, Blast_Score=335, Evalue=3e-92,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=25.8160237388724, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=25.8160237388724, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=25.8160237388724, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=58.6854460093897, Blast_Score=491, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71995829, Length=434, Percent_Identity=51.1520737327189, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=51.1520737327189, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=50, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=423, Percent_Identity=48.2269503546099, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324974, Length=423, Percent_Identity=46.3356973995272, Blast_Score=352, Evalue=7e-98,
Organism=Saccharomyces cerevisiae, GI6324969, Length=423, Percent_Identity=46.3356973995272, Blast_Score=352, Evalue=7e-98,
Organism=Saccharomyces cerevisiae, GI6323985, Length=423, Percent_Identity=46.0992907801418, Blast_Score=350, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6321968, Length=436, Percent_Identity=46.5596330275229, Blast_Score=338, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24580918, Length=436, Percent_Identity=50.4587155963303, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=436, Percent_Identity=50.4587155963303, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=436, Percent_Identity=50.4587155963303, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=436, Percent_Identity=50.4587155963303, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=436, Percent_Identity=50.4587155963303, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=436, Percent_Identity=50.4587155963303, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46088; Mature: 46088

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHKIQAREILDSRGNPTIEADVILENGIIGRASVPSGASTGSREACELRDNDPKRYAG
CCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
KGVQKAVKHVNEEINQALQGLSVSDQENLDRILCQLDNTENKSHLGANAILATSLACARA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
RALSLNQPLYMTLNQGDMMTMPVPMMNILNGGAHADNNVDIQEFMIMPIGAPDFPVALQM
HHHCCCCCEEEEECCCCEEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCCCCCHHHHH
GTEIFHVLKSVLKKQGLNTAVGDEGGFAPNIQSNRQALDLLSEAIEKAGFRLGEDIVFAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHEEHH
DVAASELFNEGFYHMSSENQKFDSHQLIEYYANLISSYPIASIEDGLDEKDWSGWKQLTT
HHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHH
LLGNKVQLVGDDLFVTNPKILQEGIAQGIANAILIKVNQIGTLSETRQAIKLAYDNGYRC
HHCCCEEEEECCEEECCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCCCEE
VMSHRSGETEDTFIADLAVASGCGQIKTGSLCRTDRTAKYNQLLRINELASLPYAGKNIL
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
KR
CC
>Mature Secondary Structure
MHIHKIQAREILDSRGNPTIEADVILENGIIGRASVPSGASTGSREACELRDNDPKRYAG
CCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
KGVQKAVKHVNEEINQALQGLSVSDQENLDRILCQLDNTENKSHLGANAILATSLACARA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
RALSLNQPLYMTLNQGDMMTMPVPMMNILNGGAHADNNVDIQEFMIMPIGAPDFPVALQM
HHHCCCCCEEEEECCCCEEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCCCCCHHHHH
GTEIFHVLKSVLKKQGLNTAVGDEGGFAPNIQSNRQALDLLSEAIEKAGFRLGEDIVFAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHEEHH
DVAASELFNEGFYHMSSENQKFDSHQLIEYYANLISSYPIASIEDGLDEKDWSGWKQLTT
HHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHH
LLGNKVQLVGDDLFVTNPKILQEGIAQGIANAILIKVNQIGTLSETRQAIKLAYDNGYRC
HHCCCEEEEECCEEECCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCCCEE
VMSHRSGETEDTFIADLAVASGCGQIKTGSLCRTDRTAKYNQLLRINELASLPYAGKNIL
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
KR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA