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The map label for this gene is aspS [H]

Identifier: 54294481

GI number: 54294481

Start: 1727682

End: 1729463

Strand: Reverse

Name: aspS [H]

Synonym: lpl1550

Alternate gene names: 54294481

Gene position: 1729463-1727682 (Counterclockwise)

Preceding gene: 54294482

Following gene: 54294480

Centisome position: 51.69

GC content: 39.9

Gene sequence:

>1782_bases
ATGCGTACACACTACTGTTCGGCCGTGAATGAATTAAGCTTGGATAAGCAAATCACAGTTTGTGGGTGGGTGCATAATAG
ACGAGATCATGGCGGAGTTATTTTTTTAGACATTCGTGATCGTTCCGGATTATTGCAAGTGGTGTATGAGCCTGAAAATA
AGGAAATTTTTGCAATTGCTGAAAAACTAAGATCAGAGTTTGTTGTTCGTGTTACTGGCATAGTCCGTAAAAGACCAGAA
GGAATGATCAATGATAAAATGGAGACTGGCAGGGTTGAAGTAATCGGTACACAACTTGAAATTCTTAATCAATCTCCAAC
TCCACCGTTTTTGCCAGATGATCATCAAGTTATCAATGAAGATTTGCGCTATAAATACCGCTATATTGATTTGCGGCGTG
CTGTTATGCAGAAGAAACTGACTTTAAGACACAAATTAAACAGTTGCATACGCAATTACTTGAATGAGCAGAATTTTTTA
GACATAGAAACGCCTATGTTAACTAAGGCAACTCCCGAAGGCGCGCGGGATTATCTTGTGCCTTCAAGGGTGCATCCAGG
GCAGTTCTATGCTTTACCTCAATCGCCACAATTATTTAAGCAATTGTTGATGATGTCAGGGTTTGATAAATATTACCAGA
TTGTACGCTGTTTTAGAGACGAAGATTTAAGGGCTGACAGACAGCCGGAATTTACGCAGCTTGATATAGAAATGGCATTT
ATTAATGAAGAAGATATATTGCAACTAATTGAAGGATTGCTCAAGATTGTATTTAAGGAAATCCTTAACATTACCTTACC
TGATAAATTGCCAAGAATGCCGTATAAAGAGGCTATGACTCGCTATGGAAGTGATAAACCTGATCTGCGTAATCCACTGG
AGTTGATTGATATTGCAGATTTGGTAAAAAATTGCGACTTTAATGTATTTGCCTCTGCTGCGAATGATAATTCAGGAAGA
GTGGTTGCTTTAAAATTACCTAATGGATGTGATCTTAGTCGAAAGGATCTGGATAACTATGGACAATTCGTAACTATATA
TGGGGCTAAAGGACTTGCATATATTAAAGTCAACGATTTAAGTGCTGGTATGGCGGGTTTGCAGTCACCAATTCTAAAGT
TCTTATCTGAAACGGCCGTGCAATCTATTCTTAATCGGGTTGAGGTGCAGACTGGCGACGTCATTTTTTTCGGTGCTGAT
AAAGCTCATGTAGTAAACGAATCTATGGGAGCCTTAAGGAATAAACTGGGTCATGATAGAAATCTGATTAATTCTGGATG
GCAATTACTGTGGGTTGTAGATTGGCCTATGTTTGAATTGGATCCTCAGAGCAATAAGTTACAGCCTATGCATCATCCCT
TTACATCCCCTCAGGAACTATCTGCCGAGGCACTTCGTTCCAAGCCAACGCAAACATTGGCCAAGGCATATGATATAGTT
ATTAATGGTTATGAAATTGGCGGTGGATCTATTCGTATCCATCAACCTGAACTACAAAAAACTGTATTTGATTTGATCGG
AATTGATGAGCAAGAGGCACATGAGAAATTTGGATTTTTACTCGATGCCTTACAATATGGTGCTCCACCTCATGGTGGAA
TTGCGTTGGGAATTGATAGATTAGCTATGCTGCTGACAGACTCAACTTCCATAAGGGATGTGATTGCGTTTCCCAAAACT
CAAACAGCATCCTGTCCTTTAACAAGTGCGCCATCGCCTGCGGGCAATGCACAACTAACTGAACTGGGTATAAGACTTGC
GCCAACTATTACGACAAAGTAA

Upstream 100 bases:

>100_bases
CATTGATGAAGCGTTAAAATTAGTAATATCGTTAGGTGTAATGCAGTCTGATCCTGCATTGGCACAAGTAAAAACTCAAT
AAACTTTAATAGGTGGTTTT

Downstream 100 bases:

>100_bases
TTTAAATTTAAGCACCACAAGTTTAAAATACTTGTGGTGCTTTTTCCTGCCTCTTTTTTTATTATTTTTTAGTTTTTCAC
TCTTTTCAGCATCGGTTAAA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MRTHYCSAVNELSLDKQITVCGWVHNRRDHGGVIFLDIRDRSGLLQVVYEPENKEIFAIAEKLRSEFVVRVTGIVRKRPE
GMINDKMETGRVEVIGTQLEILNQSPTPPFLPDDHQVINEDLRYKYRYIDLRRAVMQKKLTLRHKLNSCIRNYLNEQNFL
DIETPMLTKATPEGARDYLVPSRVHPGQFYALPQSPQLFKQLLMMSGFDKYYQIVRCFRDEDLRADRQPEFTQLDIEMAF
INEEDILQLIEGLLKIVFKEILNITLPDKLPRMPYKEAMTRYGSDKPDLRNPLELIDIADLVKNCDFNVFASAANDNSGR
VVALKLPNGCDLSRKDLDNYGQFVTIYGAKGLAYIKVNDLSAGMAGLQSPILKFLSETAVQSILNRVEVQTGDVIFFGAD
KAHVVNESMGALRNKLGHDRNLINSGWQLLWVVDWPMFELDPQSNKLQPMHHPFTSPQELSAEALRSKPTQTLAKAYDIV
INGYEIGGGSIRIHQPELQKTVFDLIGIDEQEAHEKFGFLLDALQYGAPPHGGIALGIDRLAMLLTDSTSIRDVIAFPKT
QTASCPLTSAPSPAGNAQLTELGIRLAPTITTK

Sequences:

>Translated_593_residues
MRTHYCSAVNELSLDKQITVCGWVHNRRDHGGVIFLDIRDRSGLLQVVYEPENKEIFAIAEKLRSEFVVRVTGIVRKRPE
GMINDKMETGRVEVIGTQLEILNQSPTPPFLPDDHQVINEDLRYKYRYIDLRRAVMQKKLTLRHKLNSCIRNYLNEQNFL
DIETPMLTKATPEGARDYLVPSRVHPGQFYALPQSPQLFKQLLMMSGFDKYYQIVRCFRDEDLRADRQPEFTQLDIEMAF
INEEDILQLIEGLLKIVFKEILNITLPDKLPRMPYKEAMTRYGSDKPDLRNPLELIDIADLVKNCDFNVFASAANDNSGR
VVALKLPNGCDLSRKDLDNYGQFVTIYGAKGLAYIKVNDLSAGMAGLQSPILKFLSETAVQSILNRVEVQTGDVIFFGAD
KAHVVNESMGALRNKLGHDRNLINSGWQLLWVVDWPMFELDPQSNKLQPMHHPFTSPQELSAEALRSKPTQTLAKAYDIV
INGYEIGGGSIRIHQPELQKTVFDLIGIDEQEAHEKFGFLLDALQYGAPPHGGIALGIDRLAMLLTDSTSIRDVIAFPKT
QTASCPLTSAPSPAGNAQLTELGIRLAPTITTK
>Mature_593_residues
MRTHYCSAVNELSLDKQITVCGWVHNRRDHGGVIFLDIRDRSGLLQVVYEPENKEIFAIAEKLRSEFVVRVTGIVRKRPE
GMINDKMETGRVEVIGTQLEILNQSPTPPFLPDDHQVINEDLRYKYRYIDLRRAVMQKKLTLRHKLNSCIRNYLNEQNFL
DIETPMLTKATPEGARDYLVPSRVHPGQFYALPQSPQLFKQLLMMSGFDKYYQIVRCFRDEDLRADRQPEFTQLDIEMAF
INEEDILQLIEGLLKIVFKEILNITLPDKLPRMPYKEAMTRYGSDKPDLRNPLELIDIADLVKNCDFNVFASAANDNSGR
VVALKLPNGCDLSRKDLDNYGQFVTIYGAKGLAYIKVNDLSAGMAGLQSPILKFLSETAVQSILNRVEVQTGDVIFFGAD
KAHVVNESMGALRNKLGHDRNLINSGWQLLWVVDWPMFELDPQSNKLQPMHHPFTSPQELSAEALRSKPTQTLAKAYDIV
INGYEIGGGSIRIHQPELQKTVFDLIGIDEQEAHEKFGFLLDALQYGAPPHGGIALGIDRLAMLLTDSTSIRDVIAFPKT
QTASCPLTSAPSPAGNAQLTELGIRLAPTITTK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=608, Percent_Identity=38.9802631578947, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI194272210, Length=256, Percent_Identity=30.078125, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI5031815, Length=256, Percent_Identity=30.078125, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI45439306, Length=291, Percent_Identity=28.8659793814433, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI4758762, Length=258, Percent_Identity=26.7441860465116, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788173, Length=587, Percent_Identity=55.5366269165247, Blast_Score=698, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=313, Percent_Identity=25.5591054313099, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789256, Length=295, Percent_Identity=26.1016949152542, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787161, Length=275, Percent_Identity=28.7272727272727, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=605, Percent_Identity=36.0330578512397, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI71994340, Length=266, Percent_Identity=24.4360902255639, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=259, Percent_Identity=24.7104247104247, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535927, Length=259, Percent_Identity=24.7104247104247, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17551876, Length=278, Percent_Identity=26.978417266187, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=300, Percent_Identity=25.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=620, Percent_Identity=34.5161290322581, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6323011, Length=268, Percent_Identity=29.8507462686567, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320242, Length=261, Percent_Identity=26.0536398467433, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=287, Percent_Identity=26.8292682926829, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24584738, Length=602, Percent_Identity=36.5448504983389, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24640849, Length=250, Percent_Identity=27.6, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24640851, Length=250, Percent_Identity=27.6, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17136276, Length=259, Percent_Identity=28.1853281853282, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19922416, Length=312, Percent_Identity=24.6794871794872, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66924; Mature: 66924

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTHYCSAVNELSLDKQITVCGWVHNRRDHGGVIFLDIRDRSGLLQVVYEPENKEIFAIA
CCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHH
EKLRSEFVVRVTGIVRKRPEGMINDKMETGRVEVIGTQLEILNQSPTPPFLPDDHQVINE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHEECCCCCCCCCCCCHHHHHH
DLRYKYRYIDLRRAVMQKKLTLRHKLNSCIRNYLNEQNFLDIETPMLTKATPEGARDYLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCC
PSRVHPGQFYALPQSPQLFKQLLMMSGFDKYYQIVRCFRDEDLRADRQPEFTQLDIEMAF
CCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEE
INEEDILQLIEGLLKIVFKEILNITLPDKLPRMPYKEAMTRYGSDKPDLRNPLELIDIAD
ECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHH
LVKNCDFNVFASAANDNSGRVVALKLPNGCDLSRKDLDNYGQFVTIYGAKGLAYIKVNDL
HHHCCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEEECCCEEEEEEECC
SAGMAGLQSPILKFLSETAVQSILNRVEVQTGDVIFFGADKAHVVNESMGALRNKLGHDR
CCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCH
NLINSGWQLLWVVDWPMFELDPQSNKLQPMHHPFTSPQELSAEALRSKPTQTLAKAYDIV
HHHHCCCEEEEEEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHEE
INGYEIGGGSIRIHQPELQKTVFDLIGIDEQEAHEKFGFLLDALQYGAPPHGGIALGIDR
EEEEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECHHH
LAMLLTDSTSIRDVIAFPKTQTASCPLTSAPSPAGNAQLTELGIRLAPTITTK
HHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEECCEECCC
>Mature Secondary Structure
MRTHYCSAVNELSLDKQITVCGWVHNRRDHGGVIFLDIRDRSGLLQVVYEPENKEIFAIA
CCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHH
EKLRSEFVVRVTGIVRKRPEGMINDKMETGRVEVIGTQLEILNQSPTPPFLPDDHQVINE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHEECCCCCCCCCCCCHHHHHH
DLRYKYRYIDLRRAVMQKKLTLRHKLNSCIRNYLNEQNFLDIETPMLTKATPEGARDYLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCC
PSRVHPGQFYALPQSPQLFKQLLMMSGFDKYYQIVRCFRDEDLRADRQPEFTQLDIEMAF
CCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEE
INEEDILQLIEGLLKIVFKEILNITLPDKLPRMPYKEAMTRYGSDKPDLRNPLELIDIAD
ECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHH
LVKNCDFNVFASAANDNSGRVVALKLPNGCDLSRKDLDNYGQFVTIYGAKGLAYIKVNDL
HHHCCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEEECCCEEEEEEECC
SAGMAGLQSPILKFLSETAVQSILNRVEVQTGDVIFFGADKAHVVNESMGALRNKLGHDR
CCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCH
NLINSGWQLLWVVDWPMFELDPQSNKLQPMHHPFTSPQELSAEALRSKPTQTLAKAYDIV
HHHHCCCEEEEEEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHEE
INGYEIGGGSIRIHQPELQKTVFDLIGIDEQEAHEKFGFLLDALQYGAPPHGGIALGIDR
EEEEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECHHH
LAMLLTDSTSIRDVIAFPKTQTASCPLTSAPSPAGNAQLTELGIRLAPTITTK
HHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEECCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA