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The map label for this gene is aceF [H]

Identifier: 54294454

GI number: 54294454

Start: 1696494

End: 1698128

Strand: Direct

Name: aceF [H]

Synonym: lpl1523

Alternate gene names: 54294454

Gene position: 1696494-1698128 (Clockwise)

Preceding gene: 54294453

Following gene: 54294455

Centisome position: 50.71

GC content: 37.55

Gene sequence:

>1635_bases
ATGACAAAAGAAAGTGAAATTAAAATTCCTGATATCGGTGGTGCTAATCAAGTTGATGTCATTGAGATTTTAGTGAAAGA
GGGTGATCAGATCGAAGTAGATACACCATTGATTACGCTTGAGTCTGAGAAGGCAAGCATGGACATCCCTTCTCCAATAT
CTGGAACGGTAACGCAAATTCTCGTTAAAGTCGGTGACAAAGTATCCGAAGGCGATCTCATTGTCAAAGCCAAGTCAGAT
ACAACGGCAAATATTTCCAGTTCTCAAGAACAAAAGGCTGAACCAGAAAAACAGAATTTGCAAACCAGAGCAGAAGAGCA
ATCTGTTGATACAAAGGCAACTGCATCTACACCTGGTTCCAAAGATATTGAAATAACTATCCCGGATATTGGTGGTGCAA
ATGACGTTGATGTAATTGATATTTTAGTCAAACCAGGGATGGAAGTAGAAAAGGATCAGGCATTAATTACATTAGAGGGT
GATAAAGCAACGATGGATATTCCTTCGCCCTATGCAGGTAAAGTCATCGAAATGAAAATTAAATTAGGAGATAAAGTATC
TCAAGGTACTCCTATTCTAACTCTAAAAACCCCAGGAAAATCTGAGACACCTGATATCGAGAAAAGCCAGATTAAAAATA
TCTCAGAACAATCTATAAAGGAAATAGAGAAGCCATATGAAGAAGTAAAATCCGAACCAATATCAATTAATAGCCTTGAG
ATAGCTGAATCAAAAAGTATCTTAATATCTGCAGGTCCAGCCGTAAGAAGGTTGGCAAGAGAGTTTGGAGTTGATTTATC
TTTGGTTCAGGGAAGTGGGAGAAAATCTCGTATTACAAAAGAAGATCTGCAAAATTATATCAAAGTACGTTTAAGTGAAA
AAACTACGTCTGGTGGCTTCAGCTTACCGTCAAATCCAGCCATCGATTTCAGTAAATTTGGTTCAATTGAGACAAAGCCT
TTAAATAAAATTAAAAAACTTACTGGAACAAACGTACATCGCTCCTGGATAACCATTCCACATGTCACTCAATTTGACGA
AGCAGATATTACAGACCTGGAAGCGTTTAGAAAATCAGAATCAGAGAGCGCTAAAAATCAAGATTACAAATTAACCCTGT
TAGCATTTGTTTGCAGCGTAGTTTGTAAGGCTCTCCATGCTTACCCGCAATTTAATGCGTCACTGGATGCCAGTGGTGAA
AATTTAATTTATAAAAAATATTATAATATAGGTATAGCAGTAGATACACCTAACGGTCTGGTGGTTCCTGTAATAAAGAA
TGTTGATAAATTGAGTGTAATTGATATTGCAAAGGAAATGTCCAGACTCAGTACGAAAGCTAGAGAAAAGGGATTAACAC
CTATTGATATGTCAGGCGGTTGTTTTACAATTTCCAGCCTTGGAGGTATAGGCGGAACGGCATTTACTCCTATTGTAAAT
AGCCCGGAAGTTGCAATTTTGGGCTTATCAAGATCAATAATAAAACCCATATACGATAATAAAGAGTTTAAGCCCAGATT
GATGTTACCTATTTCCCTTTCTTACGATCATAGAGTAATCGACGGCGCTGAGGCAGCCCGGTTTACTCGCTTTCTTTGTG
ATTGTTTAGGTGATATTAGAAGAGTTTTACTCTGA

Upstream 100 bases:

>100_bases
TTACAATATTAATCAAGACAAATTGGATCCAATGACCCATTAGTTAAACAATTAATCAGAACAGAAGATAGTTCCGGTTT
AACTGAGTGCTGAGGAAAAT

Downstream 100 bases:

>100_bases
CATATAAAGGAATAGAGCTATTGGTATTTCCTTGTTCTGAATGAAATGCCAATAACCCCAGAAAACATATTTTTTAATGA
GGCTTTTTATGGCTAATAAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MTKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEKASMDIPSPISGTVTQILVKVGDKVSEGDLIVKAKSD
TTANISSSQEQKAEPEKQNLQTRAEEQSVDTKATASTPGSKDIEITIPDIGGANDVDVIDILVKPGMEVEKDQALITLEG
DKATMDIPSPYAGKVIEMKIKLGDKVSQGTPILTLKTPGKSETPDIEKSQIKNISEQSIKEIEKPYEEVKSEPISINSLE
IAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKSRITKEDLQNYIKVRLSEKTTSGGFSLPSNPAIDFSKFGSIETKP
LNKIKKLTGTNVHRSWITIPHVTQFDEADITDLEAFRKSESESAKNQDYKLTLLAFVCSVVCKALHAYPQFNASLDASGE
NLIYKKYYNIGIAVDTPNGLVVPVIKNVDKLSVIDIAKEMSRLSTKAREKGLTPIDMSGGCFTISSLGGIGGTAFTPIVN
SPEVAILGLSRSIIKPIYDNKEFKPRLMLPISLSYDHRVIDGAEAARFTRFLCDCLGDIRRVLL

Sequences:

>Translated_544_residues
MTKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEKASMDIPSPISGTVTQILVKVGDKVSEGDLIVKAKSD
TTANISSSQEQKAEPEKQNLQTRAEEQSVDTKATASTPGSKDIEITIPDIGGANDVDVIDILVKPGMEVEKDQALITLEG
DKATMDIPSPYAGKVIEMKIKLGDKVSQGTPILTLKTPGKSETPDIEKSQIKNISEQSIKEIEKPYEEVKSEPISINSLE
IAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKSRITKEDLQNYIKVRLSEKTTSGGFSLPSNPAIDFSKFGSIETKP
LNKIKKLTGTNVHRSWITIPHVTQFDEADITDLEAFRKSESESAKNQDYKLTLLAFVCSVVCKALHAYPQFNASLDASGE
NLIYKKYYNIGIAVDTPNGLVVPVIKNVDKLSVIDIAKEMSRLSTKAREKGLTPIDMSGGCFTISSLGGIGGTAFTPIVN
SPEVAILGLSRSIIKPIYDNKEFKPRLMLPISLSYDHRVIDGAEAARFTRFLCDCLGDIRRVLL
>Mature_543_residues
TKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEKASMDIPSPISGTVTQILVKVGDKVSEGDLIVKAKSDT
TANISSSQEQKAEPEKQNLQTRAEEQSVDTKATASTPGSKDIEITIPDIGGANDVDVIDILVKPGMEVEKDQALITLEGD
KATMDIPSPYAGKVIEMKIKLGDKVSQGTPILTLKTPGKSETPDIEKSQIKNISEQSIKEIEKPYEEVKSEPISINSLEI
AESKSILISAGPAVRRLAREFGVDLSLVQGSGRKSRITKEDLQNYIKVRLSEKTTSGGFSLPSNPAIDFSKFGSIETKPL
NKIKKLTGTNVHRSWITIPHVTQFDEADITDLEAFRKSESESAKNQDYKLTLLAFVCSVVCKALHAYPQFNASLDASGEN
LIYKKYYNIGIAVDTPNGLVVPVIKNVDKLSVIDIAKEMSRLSTKAREKGLTPIDMSGGCFTISSLGGIGGTAFTPIVNS
PEVAILGLSRSIIKPIYDNKEFKPRLMLPISLSYDHRVIDGAEAARFTRFLCDCLGDIRRVLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=456, Percent_Identity=32.4561403508772, Blast_Score=192, Evalue=9e-49,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=35.3982300884956, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI31711992, Length=294, Percent_Identity=29.2517006802721, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI203098816, Length=310, Percent_Identity=27.741935483871, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI203098753, Length=310, Percent_Identity=27.741935483871, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI260898739, Length=147, Percent_Identity=34.6938775510204, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786305, Length=548, Percent_Identity=45.8029197080292, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=29.1764705882353, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=416, Percent_Identity=30.5288461538462, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=436, Percent_Identity=27.9816513761468, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560088, Length=434, Percent_Identity=27.6497695852535, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=28.2467532467532, Blast_Score=102, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=400, Percent_Identity=31.25, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324258, Length=343, Percent_Identity=27.4052478134111, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI18859875, Length=417, Percent_Identity=32.6139088729017, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24645909, Length=211, Percent_Identity=37.4407582938389, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=27.8969957081545, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=27.8969957081545, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 59162; Mature: 59031

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEKASMDIPSPISGTVTQI
CCCCCCEECCCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCCCHHHHHH
LVKVGDKVSEGDLIVKAKSDTTANISSSQEQKAEPEKQNLQTRAEEQSVDTKATASTPGS
HHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCC
KDIEITIPDIGGANDVDVIDILVKPGMEVEKDQALITLEGDKATMDIPSPYAGKVIEMKI
CEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEEE
KLGDKVSQGTPILTLKTPGKSETPDIEKSQIKNISEQSIKEIEKPYEEVKSEPISINSLE
EECCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEE
IAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKSRITKEDLQNYIKVRLSEKTTSGGF
ECCCCEEEEECCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHEEEECCCCCCCCC
SLPSNPAIDFSKFGSIETKPLNKIKKLTGTNVHRSWITIPHVTQFDEADITDLEAFRKSE
CCCCCCCCCHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC
SESAKNQDYKLTLLAFVCSVVCKALHAYPQFNASLDASGENLIYKKYYNIGIAVDTPNGL
HHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCE
VVPVIKNVDKLSVIDIAKEMSRLSTKAREKGLTPIDMSGGCFTISSLGGIGGTAFTPIVN
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCEECCCCC
SPEVAILGLSRSIIKPIYDNKEFKPRLMLPISLSYDHRVIDGAEAARFTRFLCDCLGDIR
CCCEEEEECCHHHHHHHHCCCCCCCEEEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHH
RVLL
HHHC
>Mature Secondary Structure 
TKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEKASMDIPSPISGTVTQI
CCCCCEECCCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCCCHHHHHH
LVKVGDKVSEGDLIVKAKSDTTANISSSQEQKAEPEKQNLQTRAEEQSVDTKATASTPGS
HHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCC
KDIEITIPDIGGANDVDVIDILVKPGMEVEKDQALITLEGDKATMDIPSPYAGKVIEMKI
CEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEEE
KLGDKVSQGTPILTLKTPGKSETPDIEKSQIKNISEQSIKEIEKPYEEVKSEPISINSLE
EECCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEE
IAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKSRITKEDLQNYIKVRLSEKTTSGGF
ECCCCEEEEECCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHEEEECCCCCCCCC
SLPSNPAIDFSKFGSIETKPLNKIKKLTGTNVHRSWITIPHVTQFDEADITDLEAFRKSE
CCCCCCCCCHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC
SESAKNQDYKLTLLAFVCSVVCKALHAYPQFNASLDASGENLIYKKYYNIGIAVDTPNGL
HHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCE
VVPVIKNVDKLSVIDIAKEMSRLSTKAREKGLTPIDMSGGCFTISSLGGIGGTAFTPIVN
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCEECCCCC
SPEVAILGLSRSIIKPIYDNKEFKPRLMLPISLSYDHRVIDGAEAARFTRFLCDCLGDIR
CCCEEEEECCHHHHHHHHCCCCCCCEEEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHH
RVLL
HHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]