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The map label for this gene is pilD [H]

Identifier: 54294433

GI number: 54294433

Start: 1671562

End: 1672425

Strand: Reverse

Name: pilD [H]

Synonym: lpl1502

Alternate gene names: 54294433

Gene position: 1672425-1671562 (Counterclockwise)

Preceding gene: 54294434

Following gene: 54294429

Centisome position: 49.99

GC content: 35.07

Gene sequence:

>864_bases
ATGATAAACGCACTAATAATCAATTATCCATGGTTTATGTATTTACTCGTTGGCCTCTTTTCTCTAGCCGTTGGAAGTTT
GCTTAATGTGATAATCTATCGCTTACCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCATTTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGTCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTGTTAAGAGGACGCTGTTACCAATGTGGTAGTCCAATTTCAATCCGCTA
CCCTTTTGTTGAGACGTTGACTACAGTTTTATCCTTATACGCCAGTTGGCACTTTGGGTTTACAATACAATTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTTATTGCTAATACCCAGAATGTATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCCTATTTAGCCTTATGGTTATTCATTAATCTGTTTTATCTGATGACAGGTAAAGTAGGTATGGGACATGGAGATTTCA
AATTATTTGCAGCATTTGGTGCCTGGCTTGGATGGATGTATTTACCATTAATTCTGCTAATATCTTCAATAACGGGGGCT
ATCATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGTCCTTTTCTTTGTATTTC
TGGATTAATAGCTATGTTTTGGGGAGACTCAATTATCAATTGGTATATAGGTTATTGGATGTAA

Upstream 100 bases:

>100_bases
GGGATTGGTGGTGGCTATGTATTTACCTATTTTCAAACTGGGTTCTGCTGTTTAAACAGATCCTATTTAATTTAATCATA
TAATATAGGCAAACGTGTCA

Downstream 100 bases:

>100_bases
AATGTTGATTAGCTTAGCGCAACCCAACAATAAATAGAGACCACACGCCTAAAATAACATGAGAAACAGATACCCCCAAA
AGAGATTTCTGTCTGTGAAA

Product: Type 4 prepilin-like proteins leader peptide processing enzyme

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MINALIINYPWFMYLLVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHFEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCGSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM

Sequences:

>Translated_287_residues
MINALIINYPWFMYLLVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHFEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCGSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM
>Mature_287_residues
MINALIINYPWFMYLLVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHFEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCGSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=272, Percent_Identity=37.5, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789732, Length=132, Percent_Identity=43.1818181818182, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 32760; Mature: 32760

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINALIINYPWFMYLLVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHFEQRKEKI
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KLNLFLPRSFCPHCKAMVKAWQNIPLLSYILLRGRCYQCGSPISIRYPFVETLTTVLSLY
EEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHCCCCCCEEECCHHHHHHHHHHHH
ASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTLGLLWIGLIANTQNVFVSLDV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCHHEEEHHH
AVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM
HHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINALIINYPWFMYLLVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHFEQRKEKI
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KLNLFLPRSFCPHCKAMVKAWQNIPLLSYILLRGRCYQCGSPISIRYPFVETLTTVLSLY
EEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHCCCCCCEEECCHHHHHHHHHHHH
ASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTLGLLWIGLIANTQNVFVSLDV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCHHEEEHHH
AVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM
HHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9529113 [H]