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The map label for this gene is artJ [H]

Identifier: 54294402

GI number: 54294402

Start: 1640582

End: 1641298

Strand: Direct

Name: artJ [H]

Synonym: lpl1471

Alternate gene names: 54294402

Gene position: 1640582-1641298 (Clockwise)

Preceding gene: 54294401

Following gene: 54294404

Centisome position: 49.04

GC content: 31.24

Gene sequence:

>717_bases
ATGAAGTTACTACAACTTCTCTTATTTATTATTATTTCCTCTAATTCTTATTCATTAAATTTAACAATTGGTACTTCAAA
ATTTAATCCGCCTTTTGAAGTTTGGTCTGGTAACAACAGTTCATTGTATGGCTTTGATATTGACCTTATGCAAGAAATAT
GTAGAAGATTACATGCAACCTGTACTTTTGAAGCCTATATATTCGATGATTTATTTCCCGCGTTAAAAAAACGCGAAGTC
GATCTGGTTATTGCCTCAATGATTATTACAGATGAAAGAAAGAAACACTTTATTTTTAGTCTTCCCTATATGGAAAGTAA
CTCACAATATATAACTACCGTTGATTCTAAAATCAGCACATTTGATGATTTGCACGGGAAAAAAATTGGAGTAAGAAAAG
GAACTCCTTATGCAAGGCAAGTTTTGTCAGAAAACCGCAACAATCAGGTAATTTTTTATGAGCTCATACAAGATATGCTT
CTTGGTTTAAGCAATAATGAAGTTGATGCCAGTTTAATGGATTATGAAGCAGCAAAATATTGGATGGCATCTGAGCCCTA
TGCTTATAAATTAATTGGTAAGAAAATTTCTATTGGAGAAGGGTATTCTATTATGGCAAACCCTGATCAATTTGTATTAA
TTAAAAAAATAAATAAAATTCTTCTTGAATTGGAAGCTGATGGAGCTTATCTGAAACTATATTCAGAGTACTTTTAG

Upstream 100 bases:

>100_bases
AAACCAAATTAGAGAAATCGTATGCTACAAATTGTACAAATCAAATTTTTTGTACCATGATTATGTTTAAACAAACTTCC
TAAATTACAGGATATAGTTT

Downstream 100 bases:

>100_bases
AAGAGGAGTATTAAGGACAAATAGCACCCGAAATACTCCAATTATACCTAAACAGGCTCCATTGTTTTTTTATTGACCGA
GTTTTTTTGTTCCTTTTCTT

Product: hypothetical protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHATCTFEAYIFDDLFPALKKREV
DLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKISTFDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDML
LGLSNNEVDASLMDYEAAKYWMASEPYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLELEADGAYLKLYSEYF

Sequences:

>Translated_238_residues
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHATCTFEAYIFDDLFPALKKREV
DLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKISTFDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDML
LGLSNNEVDASLMDYEAAKYWMASEPYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLELEADGAYLKLYSEYF
>Mature_238_residues
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHATCTFEAYIFDDLFPALKKREV
DLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKISTFDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDML
LGLSNNEVDASLMDYEAAKYWMASEPYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLELEADGAYLKLYSEYF

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=239, Percent_Identity=33.0543933054393, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1787088, Length=213, Percent_Identity=33.8028169014084, Blast_Score=133, Evalue=9e-33,
Organism=Escherichia coli, GI1788649, Length=249, Percent_Identity=32.1285140562249, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=28.8793103448276, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788228, Length=227, Percent_Identity=29.0748898678414, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787031, Length=240, Percent_Identity=27.0833333333333, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27381; Mature: 27381

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHAT
CHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCEEEEECHHHHHHHHHHHCCE
CTFEAYIFDDLFPALKKREVDLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKIST
EEEEHHHHHHHHHHHHHCCHHHEEEEHHHCCCCCCEEEEECCEECCCCCEEEEECCHHHH
FDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDMLLGLSNNEVDASLMDYEAAKY
HHHCCCCEEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHH
WMASEPYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLELEADGAYLKLYSEYF
CCCCCCEEEEEECCEEECCCCEEEEECCCCEEHHHHHHHHEEEECCCCCEEEEEECCC
>Mature Secondary Structure
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHAT
CHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCEEEEECHHHHHHHHHHHCCE
CTFEAYIFDDLFPALKKREVDLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKIST
EEEEHHHHHHHHHHHHHCCHHHEEEEHHHCCCCCCEEEEECCEECCCCCEEEEECCHHHH
FDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDMLLGLSNNEVDASLMDYEAAKY
HHHCCCCEEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHH
WMASEPYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLELEADGAYLKLYSEYF
CCCCCCEEEEEECCEEECCCCEEEEECCCCEEHHHHHHHHEEEECCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]