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The map label for this gene is cmr [H]

Identifier: 54294291

GI number: 54294291

Start: 1511391

End: 1512671

Strand: Reverse

Name: cmr [H]

Synonym: lpl1356

Alternate gene names: 54294291

Gene position: 1512671-1511391 (Counterclockwise)

Preceding gene: 54294293

Following gene: 54294275

Centisome position: 45.21

GC content: 37.94

Gene sequence:

>1281_bases
ATGTCTGAACCACTAATTAAAATTTCATACAGACAGGCCATTATCTTTGCTTGCTTCCTGGTTTTATATGAGTTTTTGAC
TTATATAGCCAATGACATGATCATGCCAGGTATGATCAATGTTGTTAAATCATTCAACGCGCATGAAAGTGTTGTTGCCA
CCTCTCTAACTATCTATGTCCTCGGAGGGGCCAGTTTGCAGCTCATACTTGGCCCACTATCAGATGCTTATGGCAGAAGA
CCTATGATGTTAATAGGATCATGCTTATTTTTTCTGTTCACTCTTTTGATTGCAAGCTCACACTCCATGAATCAGTTTTT
AATCGCCCGTTTTTTTCAGGGAATGGGATTGTGTTTCATTGGCGTTATAGGCTATGCCACAATTCAAGAAATATTTGAAG
AAATGGACGCTATCCGATTAATTGCTATCATGGCTAATGCCGCAATATTAGCCCCATTATTAGGGCCACTATTAGGAGCC
ATAATCATTCATTATGCGTCCTGGCGTTTTATTTTTATTATTATTGCTCTGGGTGCTCTTTTAGCTTATTGGGGATTATG
GCGGTTTATGCCAGAACCCATAGGCCAGATAAAAAGAGACGGCCAGCTTATACCTAAAACACCGTTCGCACTGAACGCTA
TAACCCGAAATTATAAAACGTTATTATCTAATCAGGCCTTTTGTTATAGTGCAATTGCTGAAGGTCTGGTAGGGATCCCA
TGCATCGCCTGGATTGCTCTGGCACCAATCATATTGATTGCTGAAGCAAAATTAACTGTAATTCAATATGGCCTTTGGCA
ACTACCCATCTTCGGGGCAACAATTTTAGGAAACTGGTGCTTACATCATTTAACTTACAAATATAAAATCGAGAGAATTA
TTTTTATAGGTTGTATCATAATGATCGTGGGACTTGCTCTGACAGCTCTTCTCCCTTATTTTTATGGCAATAATTATGTG
TATTTGATCCCGGGAATCATAATTTACTTTTTCTCACTGAGTGTAATTAATGCACCCTTGAACAGGTATTGTTTATTTGT
AACCGCTGTAAGCAAAGGAACAGCCTCGGCATTAATCAGCCTAAGTATTATGATCATTGGGGCTATTGGAATAGAAATAG
CTAACCTGTTTTATCAACATCACAATAATCTGCATTTCGCCCTCTATTGTAATGCCGTTGGATTATTATTTCTAATCTTT
ATCGGTTTAACTTTTTTTATAGGGACTCCCAAGAAATCTGAAATTCATGATGAAGCAAATAGTAATGTGGCTAACTCATG
A

Upstream 100 bases:

>100_bases
TTACTTACCAAAACATTTATCAGATTTTCTACCAAATTATGAAATAAACGTATATACTGATAGACTGCCTTTTAGAAACG
ATTGATTCAGGGTTAATGCT

Downstream 100 bases:

>100_bases
GAACAAGCCACATTGACATAAACTGATTACTTTGTTTTAGTCAATAACGCAAAATTGAATTGATAAATCTGTTATTAATC
TCTGCTCGCAGGATGCGATT

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKSFNAHESVVATSLTIYVLGGASLQLILGPLSDAYGRR
PMMLIGSCLFFLFTLLIASSHSMNQFLIARFFQGMGLCFIGVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGA
IIIHYASWRFIFIIIALGALLAYWGLWRFMPEPIGQIKRDGQLIPKTPFALNAITRNYKTLLSNQAFCYSAIAEGLVGIP
CIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATILGNWCLHHLTYKYKIERIIFIGCIIMIVGLALTALLPYFYGNNYV
YLIPGIIIYFFSLSVINAPLNRYCLFVTAVSKGTASALISLSIMIIGAIGIEIANLFYQHHNNLHFALYCNAVGLLFLIF
IGLTFFIGTPKKSEIHDEANSNVANS

Sequences:

>Translated_426_residues
MSEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKSFNAHESVVATSLTIYVLGGASLQLILGPLSDAYGRR
PMMLIGSCLFFLFTLLIASSHSMNQFLIARFFQGMGLCFIGVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGA
IIIHYASWRFIFIIIALGALLAYWGLWRFMPEPIGQIKRDGQLIPKTPFALNAITRNYKTLLSNQAFCYSAIAEGLVGIP
CIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATILGNWCLHHLTYKYKIERIIFIGCIIMIVGLALTALLPYFYGNNYV
YLIPGIIIYFFSLSVINAPLNRYCLFVTAVSKGTASALISLSIMIIGAIGIEIANLFYQHHNNLHFALYCNAVGLLFLIF
IGLTFFIGTPKKSEIHDEANSNVANS
>Mature_425_residues
SEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKSFNAHESVVATSLTIYVLGGASLQLILGPLSDAYGRRP
MMLIGSCLFFLFTLLIASSHSMNQFLIARFFQGMGLCFIGVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGAI
IIHYASWRFIFIIIALGALLAYWGLWRFMPEPIGQIKRDGQLIPKTPFALNAITRNYKTLLSNQAFCYSAIAEGLVGIPC
IAWIALAPIILIAEAKLTVIQYGLWQLPIFGATILGNWCLHHLTYKYKIERIIFIGCIIMIVGLALTALLPYFYGNNYVY
LIPGIIIYFFSLSVINAPLNRYCLFVTAVSKGTASALISLSIMIIGAIGIEIANLFYQHHNNLHFALYCNAVGLLFLIFI
GLTFFIGTPKKSEIHDEANSNVANS

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=407, Percent_Identity=41.2776412776413, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1790794, Length=416, Percent_Identity=32.6923076923077, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI48994889, Length=157, Percent_Identity=33.1210191082803, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788509, Length=248, Percent_Identity=25, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082312, Length=309, Percent_Identity=25.2427184466019, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790146, Length=146, Percent_Identity=24.6575342465753, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319480, Length=192, Percent_Identity=27.6041666666667, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322071, Length=204, Percent_Identity=21.5686274509804, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321838, Length=207, Percent_Identity=28.9855072463768, Blast_Score=66, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=222, Percent_Identity=22.0720720720721, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47218; Mature: 47087

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKSFNAHESVVATSLTIYV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHEEEEE
LGGASLQLILGPLSDAYGRRPMMLIGSCLFFLFTLLIASSHSMNQFLIARFFQGMGLCFI
ECCCEEEEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
GVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGAIIIHYASWRFIFIIIALGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYWGLWRFMPEPIGQIKRDGQLIPKTPFALNAITRNYKTLLSNQAFCYSAIAEGLVGIP
HHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
CIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATILGNWCLHHLTYKYKIERIIFIGCII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIVGLALTALLPYFYGNNYVYLIPGIIIYFFSLSVINAPLNRYCLFVTAVSKGTASALIS
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCHHHHEEEEEEECCCHHHHHHH
LSIMIIGAIGIEIANLFYQHHNNLHFALYCNAVGLLFLIFIGLTFFIGTPKKSEIHDEAN
HHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHC
SNVANS
CCCCCC
>Mature Secondary Structure 
SEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKSFNAHESVVATSLTIYV
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHEEEEE
LGGASLQLILGPLSDAYGRRPMMLIGSCLFFLFTLLIASSHSMNQFLIARFFQGMGLCFI
ECCCEEEEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
GVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGAIIIHYASWRFIFIIIALGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYWGLWRFMPEPIGQIKRDGQLIPKTPFALNAITRNYKTLLSNQAFCYSAIAEGLVGIP
HHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
CIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATILGNWCLHHLTYKYKIERIIFIGCII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIVGLALTALLPYFYGNNYVYLIPGIIIYFFSLSVINAPLNRYCLFVTAVSKGTASALIS
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCHHHHEEEEEEECCCHHHHHHH
LSIMIIGAIGIEIANLFYQHHNNLHFALYCNAVGLLFLIFIGLTFFIGTPKKSEIHDEAN
HHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHC
SNVANS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]