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The map label for this gene is holB [H]

Identifier: 54294286

GI number: 54294286

Start: 1506540

End: 1507445

Strand: Direct

Name: holB [H]

Synonym: lpl1351

Alternate gene names: 54294286

Gene position: 1506540-1507445 (Clockwise)

Preceding gene: 54294285

Following gene: 54294287

Centisome position: 45.03

GC content: 35.76

Gene sequence:

>906_bases
ATGATTACTTATCAAGAACAATGGCAGAAAATGCAAATGGCCTTGGCGATAAGACGAATTCCTCAAGCCATGCTATTTTT
GGGTTCTTTGCATTGTGGTCTTATAGAATTTACAAATCAAGTGATCAAATTACTCTTGTGTAAAATAAAACAGAATGAGC
CTTGTTTGACTTGCATGGATTGCCAAATGATAGAGCGTTTTGAGCATCCGGATGTGGAATGGGTTAAGCCTGAAAGAAGC
GGTGGTATTATTAAAATTGATCAAATTAGGGGATTGCAGAGTACGGCGTTTCTAACACCACAGAGGACTAATCATAAACT
TATAATTATCGAATCAGCTGAATCAATGAATACTGCGGCATCAAATGCCTTATTGAAAATTCTGGAAGAACCAGCGCAAC
ATACTGTCTTTATTTTGTTAGCGCAACAAATTGGTAATATACCACCCACCGTACTGAGCCGATGCCAAATGACGCAATTT
GCTGTTTTTGATGAGTTGCCTTGCAACAATTTATTGGCTTTAGGAGAGTACTATACTCAGGAATCGAATAGCACAATGGT
ATTCAAGCATTCAGAATCCATTTTAGAAGGCTTGATTGCAATTATTGAGCGAAGAGAGCATCCATGCACTTTGGCTGCAC
AATGGATTCAACATGAATTAAGTTCTTTTTTGTGGTTTTTATATTTGCTCTATTCTCAACTGCAGTATATGCATTTGTTT
CGAGTGGCTTATTCTGGTACGTTAACAAAACAATTGAATCAGCTATCCTCATTACTCAATCCGGTTATGATTTTTGCCCA
AGTAGATAAAATCAATACTTTGTTAAGAAAATTAAGCCATAATATGAATATGAATCATACATTGGTCTTGGAAGATTTAT
TATTTTCTTTGGTTGTTGATTATTAA

Upstream 100 bases:

>100_bases
AACAAAACCCGGAAATTATGATGATTGATGCTAACAGGTCTTTGGAAGAGGTTCAATCTTCCATTCAATCAGTAATCGAG
GAGTTTATAGAGCATAACCT

Downstream 100 bases:

>100_bases
TTGAGTTTAAAGGAATTAAACCATTGCGTGTGAACCGAGTTTTTATTAAGCAACTGAAGTGAGCAAGATGATTAGTTAAA
ACAGGACTATTCAAGGAGGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKLLLCKIKQNEPCLTCMDCQMIERFEHPDVEWVKPERS
GGIIKIDQIRGLQSTAFLTPQRTNHKLIIIESAESMNTAASNALLKILEEPAQHTVFILLAQQIGNIPPTVLSRCQMTQF
AVFDELPCNNLLALGEYYTQESNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQHELSSFLWFLYLLYSQLQYMHLF
RVAYSGTLTKQLNQLSSLLNPVMIFAQVDKINTLLRKLSHNMNMNHTLVLEDLLFSLVVDY

Sequences:

>Translated_301_residues
MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKLLLCKIKQNEPCLTCMDCQMIERFEHPDVEWVKPERS
GGIIKIDQIRGLQSTAFLTPQRTNHKLIIIESAESMNTAASNALLKILEEPAQHTVFILLAQQIGNIPPTVLSRCQMTQF
AVFDELPCNNLLALGEYYTQESNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQHELSSFLWFLYLLYSQLQYMHLF
RVAYSGTLTKQLNQLSSLLNPVMIFAQVDKINTLLRKLSHNMNMNHTLVLEDLLFSLVVDY
>Mature_301_residues
MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKLLLCKIKQNEPCLTCMDCQMIERFEHPDVEWVKPERS
GGIIKIDQIRGLQSTAFLTPQRTNHKLIIIESAESMNTAASNALLKILEEPAQHTVFILLAQQIGNIPPTVLSRCQMTQF
AVFDELPCNNLLALGEYYTQESNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQHELSSFLWFLYLLYSQLQYMHLF
RVAYSGTLTKQLNQLSSLLNPVMIFAQVDKINTLLRKLSHNMNMNHTLVLEDLLFSLVVDY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=143, Percent_Identity=30.0699300699301, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34688; Mature: 34688

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKLLLCKIKQNEPCLTCMD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHH
CQMIERFEHPDVEWVKPERSGGIIKIDQIRGLQSTAFLTPQRTNHKLIIIESAESMNTAA
HHHHHHCCCCCCCEECCCCCCCEEEEHHHCCCCHHEEECCCCCCCEEEEEECCCHHHHHH
SNALLKILEEPAQHTVFILLAQQIGNIPPTVLSRCQMTQFAVFDELPCNNLLALGEYYTQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
ESNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQHELSSFLWFLYLLYSQLQYMHLF
CCCCEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVAYSGTLTKQLNQLSSLLNPVMIFAQVDKINTLLRKLSHNMNMNHTLVLEDLLFSLVVD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
Y
C
>Mature Secondary Structure
MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKLLLCKIKQNEPCLTCMD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHH
CQMIERFEHPDVEWVKPERSGGIIKIDQIRGLQSTAFLTPQRTNHKLIIIESAESMNTAA
HHHHHHCCCCCCCEECCCCCCCEEEEHHHCCCCHHEEECCCCCCCEEEEEECCCHHHHHH
SNALLKILEEPAQHTVFILLAQQIGNIPPTVLSRCQMTQFAVFDELPCNNLLALGEYYTQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
ESNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQHELSSFLWFLYLLYSQLQYMHLF
CCCCEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVAYSGTLTKQLNQLSSLLNPVMIFAQVDKINTLLRKLSHNMNMNHTLVLEDLLFSLVVD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]