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The map label for this gene is rnhB [H]

Identifier: 54294259

GI number: 54294259

Start: 1481100

End: 1481675

Strand: Direct

Name: rnhB [H]

Synonym: lpl1324

Alternate gene names: 54294259

Gene position: 1481100-1481675 (Clockwise)

Preceding gene: 54294255

Following gene: 54294265

Centisome position: 44.27

GC content: 40.8

Gene sequence:

>576_bases
ATGAATACTGAGCTAAAAATATTGATGGCTGGGGTCGATGAAGTGGGGAGAGGGCCACTTGCGGGTGCCGTTGTAACTGC
AGCCGTTATTTTAAAAAAACCAATAGATGGGCTTACCGACTCAAAAAAATTAAGCCCAAAACAAAGAAATTTATTAGCAA
TTAGAATAAAGGAAGAAGCGTTAGCTTTTGCCTATGGGCGAGCAGAAGTTGAGGAAATAGATCAATTAAATATTCATCAT
GCTACTCTATTAGCTATGCGAAGGGCAGTTGAAGCTTTACCCATTCAGCCAGATAATGTCCTTGTCGATGGCGCTTTTAC
TCCTCAATTGAACATGCCATGCAAGGCTATTGTCCAGGGAGATAGCTTGATACCTGAAATTAGTGCGGCATCCATCCTCG
CCAAGGTATTGCGTGATGAAGAAATGGTAGCTCTTGATAAAATATATCCAGGATATGGTTTTGCAGAACATAAAGGTTAT
GCAACCCCGGTTCATAAAGAAGCGCTAATGCGTTTGGGTCCTTGTAAAATACATAGAAGAAGTTACTCTCCTGTTGCGGA
TTTGATTTCAAAGTAA

Upstream 100 bases:

>100_bases
AACTCCTGAAATTGGTTCAAATCAAAGAAATCGATTCTTGTCTTGCACTTGCATTCAATAGCTATTATCTTACGCCTAAT
TTTTCGCAGAGAGCAGTTAA

Downstream 100 bases:

>100_bases
AATTCTGAGTGGTATTAATTTGGAAAATACCGTTCAAATAAAAATACATAAGAAATAGCTTTTAAAATGTCATAATGTGA
TTTATACAAATCAGAGTATA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKLSPKQRNLLAIRIKEEALAFAYGRAEVEEIDQLNIHH
ATLLAMRRAVEALPIQPDNVLVDGAFTPQLNMPCKAIVQGDSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGY
ATPVHKEALMRLGPCKIHRRSYSPVADLISK

Sequences:

>Translated_191_residues
MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKLSPKQRNLLAIRIKEEALAFAYGRAEVEEIDQLNIHH
ATLLAMRRAVEALPIQPDNVLVDGAFTPQLNMPCKAIVQGDSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGY
ATPVHKEALMRLGPCKIHRRSYSPVADLISK
>Mature_191_residues
MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKLSPKQRNLLAIRIKEEALAFAYGRAEVEEIDQLNIHH
ATLLAMRRAVEALPIQPDNVLVDGAFTPQLNMPCKAIVQGDSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGY
ATPVHKEALMRLGPCKIHRRSYSPVADLISK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=180, Percent_Identity=63.3333333333333, Blast_Score=214, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 20786; Mature: 20786

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKLSPKQRNLLAIRIKEEA
CCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHH
LAFAYGRAEVEEIDQLNIHHATLLAMRRAVEALPIQPDNVLVDGAFTPQLNMPCKAIVQG
HHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHEEECC
DSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGYATPVHKEALMRLGPCKIHRR
CCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHEECC
SYSPVADLISK
CCCHHHHHHCC
>Mature Secondary Structure
MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKLSPKQRNLLAIRIKEEA
CCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHH
LAFAYGRAEVEEIDQLNIHHATLLAMRRAVEALPIQPDNVLVDGAFTPQLNMPCKAIVQG
HHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHEEECC
DSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGYATPVHKEALMRLGPCKIHRR
CCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHEECC
SYSPVADLISK
CCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA