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The map label for this gene is folC [H]

Identifier: 54294230

GI number: 54294230

Start: 1446808

End: 1448094

Strand: Direct

Name: folC [H]

Synonym: lpl1295

Alternate gene names: 54294230

Gene position: 1446808-1448094 (Clockwise)

Preceding gene: 54294229

Following gene: 54294231

Centisome position: 43.24

GC content: 38.69

Gene sequence:

>1287_bases
TTGCACACGCCATGGAGTGAATGGGATTTAAGTCACTGGTTGACTCATTTGGAAACCAGAAATACCCGGGAAATACAGCT
TGGTTTGACCAGAGTTTTAACTGTAGCGCAGAAATTAAATTTGCAGCGGCCAAATTGCAAGGTAATAACCGTTGCAGGAA
CAAACGGTAAAGGTTCTACAGTCACAGCACTTGAAACCATCTATCACCAGTCAGGTTTTAGAGTTGGTACCTATACGTCG
CCCCATCTTCTTGCGTTTAATGAACGTATTCGAATTAATTTAGCACCGATTCAAGATAAAGATTTATGTCAGGCTTTTTC
TGTGATTGAAGAAGCGCGTTACCCTACCAATTTAACCTATTTTGAAATGGCAACACTTGCTGCCTTATGGTATTTCAAAC
AGCATGGCCTGGATCTGATAATTCTTGAAGTAGGCTTGGGAGGACGGTTGGATGCTACAAACATTGTTGATACTGATTTA
GCGATCATTACTACTATCGACTTTGATCATCAGAATTTTCTTGGTGACACACTTGAGTTGATAGGGTATGAGAAAGCCGG
AATACTACGAAAAAATAAACCTTTTATCTATGCGGATCTCTCACCACCAAAAAGTGTCGTTGAGTTTGCGGTACAACTTG
GAGCATCATCTTATTTATACGGTAAAGACTATTTTTATCAGGAAGAAGCAGACCATTGGGGGGTTACTTGTCAAGGTAAG
CCATTTGAGCAATTGACCAGGCCAAAAATTCAACTTCGTTCTGCGGCAGCAGCTGTAATGGCTTGTCATTTGATGCAAAA
TGATTTGCCTGTATCCTATAAGAATCTAAAGAAAGCGATGGAGCTCATTTATATCCCGGGGCGATTGCAATTAGAAAAAG
GAAAGACTGATATTTTGTATGATGTATCTCATAATCCGCAGTCAGTTCGATTACTTGCTGATACAGTAAAAAAAATCAAT
GTAAAAGGGCAAATTCATGCTGTTTTCTCCGCTTTAAAGGATAAAGATCTTTATGGATTGATTATGCCTTTAAGGGATTG
TGTTAATCGATGGTATCCTGCACAATTACATAATAAGCGCGCTGCAAGTCAAGAGGAATTGCTTTCTGTATTCAGGGAAG
CAGAAATTTTTCTAGATGTTTGTTATAATGACCCATTTACAGCTTTTGAAATGGCATTAAAGCAAGCATCAGATGACGAT
TTAATTATTGTTTATGGTTCATTTTTTACGGTTAGTCATGTAATGGCTGCCCAACATAATCTTTTGGAGCAGAAGGAGAA
TCAATGA

Upstream 100 bases:

>100_bases
GAACATGGGCATATTGATATGATAGTTGAACGTAAACATCTTCGAGCAACTATATCTGAATTGGTTTCCAAATTGACTCA
TAAAGGAGCGGAGAGTTTTT

Downstream 100 bases:

>100_bases
AACTGATAATGGATGAGAAAGTTAAACATCGCTTGATAGGTTTGGCCGTTATTATTTCATTGGGGGCAATTTTTGCTCCT
GCGCTAATAAAAAAATCCAG

Product: hypothetical protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MHTPWSEWDLSHWLTHLETRNTREIQLGLTRVLTVAQKLNLQRPNCKVITVAGTNGKGSTVTALETIYHQSGFRVGTYTS
PHLLAFNERIRINLAPIQDKDLCQAFSVIEEARYPTNLTYFEMATLAALWYFKQHGLDLIILEVGLGGRLDATNIVDTDL
AIITTIDFDHQNFLGDTLELIGYEKAGILRKNKPFIYADLSPPKSVVEFAVQLGASSYLYGKDYFYQEEADHWGVTCQGK
PFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNLKKAMELIYIPGRLQLEKGKTDILYDVSHNPQSVRLLADTVKKIN
VKGQIHAVFSALKDKDLYGLIMPLRDCVNRWYPAQLHNKRAASQEELLSVFREAEIFLDVCYNDPFTAFEMALKQASDDD
LIIVYGSFFTVSHVMAAQHNLLEQKENQ

Sequences:

>Translated_428_residues
MHTPWSEWDLSHWLTHLETRNTREIQLGLTRVLTVAQKLNLQRPNCKVITVAGTNGKGSTVTALETIYHQSGFRVGTYTS
PHLLAFNERIRINLAPIQDKDLCQAFSVIEEARYPTNLTYFEMATLAALWYFKQHGLDLIILEVGLGGRLDATNIVDTDL
AIITTIDFDHQNFLGDTLELIGYEKAGILRKNKPFIYADLSPPKSVVEFAVQLGASSYLYGKDYFYQEEADHWGVTCQGK
PFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNLKKAMELIYIPGRLQLEKGKTDILYDVSHNPQSVRLLADTVKKIN
VKGQIHAVFSALKDKDLYGLIMPLRDCVNRWYPAQLHNKRAASQEELLSVFREAEIFLDVCYNDPFTAFEMALKQASDDD
LIIVYGSFFTVSHVMAAQHNLLEQKENQ
>Mature_428_residues
MHTPWSEWDLSHWLTHLETRNTREIQLGLTRVLTVAQKLNLQRPNCKVITVAGTNGKGSTVTALETIYHQSGFRVGTYTS
PHLLAFNERIRINLAPIQDKDLCQAFSVIEEARYPTNLTYFEMATLAALWYFKQHGLDLIILEVGLGGRLDATNIVDTDL
AIITTIDFDHQNFLGDTLELIGYEKAGILRKNKPFIYADLSPPKSVVEFAVQLGASSYLYGKDYFYQEEADHWGVTCQGK
PFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNLKKAMELIYIPGRLQLEKGKTDILYDVSHNPQSVRLLADTVKKIN
VKGQIHAVFSALKDKDLYGLIMPLRDCVNRWYPAQLHNKRAASQEELLSVFREAEIFLDVCYNDPFTAFEMALKQASDDD
LIIVYGSFFTVSHVMAAQHNLLEQKENQ

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=155, Percent_Identity=37.4193548387097, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI66932990, Length=155, Percent_Identity=37.4193548387097, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1788654, Length=413, Percent_Identity=38.9830508474576, Blast_Score=287, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI17553150, Length=346, Percent_Identity=26.878612716763, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=346, Percent_Identity=26.878612716763, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71984923, Length=346, Percent_Identity=26.878612716763, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323760, Length=344, Percent_Identity=30.8139534883721, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324815, Length=300, Percent_Identity=27.6666666666667, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24641571, Length=165, Percent_Identity=33.9393939393939, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24581568, Length=207, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 48589; Mature: 48589

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTPWSEWDLSHWLTHLETRNTREIQLGLTRVLTVAQKLNLQRPNCKVITVAGTNGKGST
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
VTALETIYHQSGFRVGTYTSPHLLAFNERIRINLAPIQDKDLCQAFSVIEEARYPTNLTY
HHHHHHHHHHCCCEEECCCCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCHH
FEMATLAALWYFKQHGLDLIILEVGLGGRLDATNIVDTDLAIITTIDFDHQNFLGDTLEL
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEEECCCCHHHHHHHHH
IGYEKAGILRKNKPFIYADLSPPKSVVEFAVQLGASSYLYGKDYFYQEEADHWGVTCQGK
HCCCHHCCEECCCCEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEECCC
PFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNLKKAMELIYIPGRLQLEKGKTDILY
CHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEECCCEEEEECCCCEEEE
DVSHNPQSVRLLADTVKKINVKGQIHAVFSALKDKDLYGLIMPLRDCVNRWYPAQLHNKR
ECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHC
AASQEELLSVFREAEIFLDVCYNDPFTAFEMALKQASDDDLIIVYGSFFTVSHVMAAQHN
CCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
LLEQKENQ
HHHHCCCC
>Mature Secondary Structure
MHTPWSEWDLSHWLTHLETRNTREIQLGLTRVLTVAQKLNLQRPNCKVITVAGTNGKGST
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
VTALETIYHQSGFRVGTYTSPHLLAFNERIRINLAPIQDKDLCQAFSVIEEARYPTNLTY
HHHHHHHHHHCCCEEECCCCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCHH
FEMATLAALWYFKQHGLDLIILEVGLGGRLDATNIVDTDLAIITTIDFDHQNFLGDTLEL
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEEECCCCHHHHHHHHH
IGYEKAGILRKNKPFIYADLSPPKSVVEFAVQLGASSYLYGKDYFYQEEADHWGVTCQGK
HCCCHHCCEECCCCEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEECCC
PFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNLKKAMELIYIPGRLQLEKGKTDILY
CHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEECCCEEEEECCCCEEEE
DVSHNPQSVRLLADTVKKINVKGQIHAVFSALKDKDLYGLIMPLRDCVNRWYPAQLHNKR
ECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHC
AASQEELLSVFREAEIFLDVCYNDPFTAFEMALKQASDDDLIIVYGSFFTVSHVMAAQHN
CCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
LLEQKENQ
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]