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The map label for this gene is flgJ [H]

Identifier: 54294168

GI number: 54294168

Start: 1371668

End: 1372543

Strand: Direct

Name: flgJ [H]

Synonym: lpl1232

Alternate gene names: 54294168

Gene position: 1371668-1372543 (Clockwise)

Preceding gene: 54294167

Following gene: 54294169

Centisome position: 41.0

GC content: 37.44

Gene sequence:

>876_bases
ATGACCATACAAAGTATTGCTACGAGTGATTTTCAAGGATTAAATGAATTAAAAGTCCAGGCAAAAAACAATGCAAAAGA
AGCATTGCCTGAAGTAGCTAAACAGTTTGAAGGAATATTCTTGCAATCCATGTTAAAAAGCATGAGGATGGGGCTGCATT
TCCTTGATGAATCAAGTCCTTTTAGTGGTAAGAATGAAGCAACTTTTCAAGAGATGCTGGATACACAATATGCTAGCACT
ATTGCCGAGTCTAAAGGAATAGGATTGGCAGCTCTATTGGCTAAACAACTGGAAAATTCAGTTGGGGATAAAGCAAACAA
TCCAGTTAACTCTTCTACAGAGGTTAGCAATACTAAAGTAACTAATTCAGAAGAATCGCTATCTGTTGTTGATGATTTCG
TAAAATCTGTGTGGCCCACAGCAAAACAGGCTGCATTATTAATTGGATTGGATCCCAAGCTGTTGGTAGCGCAAGCGGCA
TTAGAGACTGGCTGGGGTAAATTTGTTACAAGAGATGCAGATGGTTCAAGCAGTAATAATTTATTTAATATAAAAACCGG
ATCGCATAGCGAAGTAGAGTCCATTCAGGTTAAAACAACAGAATACATTGCCGATACACCCATTAAGATCAATGCTTCCT
TTAGAAAATATCCATCCATAGAGCATAGTTTTCATGACTATATTTCATTAATTAAGGGAAGTGAGCGATACCAGATGGCC
TTGGCCAATGCAGAAAATCCTGAGATTTATGTCAGTGAGCTTAATAAAGCAGGGTATGCTACTGACCCAAATTATAGTAA
TAAGATTTTGTCTATTTATCATGGTGATGAATTGAACCAGGCAATACAACGATGTGAATCATCCGAACAAAGTTGA

Upstream 100 bases:

>100_bases
GCTGTTGGTGCAACACCTGCTGATGTGATTTCCATACTGGAAGCTTTACAACAGGCCGGAGCATTGAGTGCTACATTAAT
AGTCATATAAAGGGATGGAT

Downstream 100 bases:

>100_bases
TAGTTAGGCCCAAGTTAAAGAAAAAATTAGTTCAGGGAGTGAACAAAATATGTCTATACTTAATATTGCCTATTCAGGAT
TAAATGCTTTTCAGCGTGCC

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTIQSIATSDFQGLNELKVQAKNNAKEALPEVAKQFEGIFLQSMLKSMRMGLHFLDESSPFSGKNEATFQEMLDTQYAST
IAESKGIGLAALLAKQLENSVGDKANNPVNSSTEVSNTKVTNSEESLSVVDDFVKSVWPTAKQAALLIGLDPKLLVAQAA
LETGWGKFVTRDADGSSSNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYPSIEHSFHDYISLIKGSERYQMA
LANAENPEIYVSELNKAGYATDPNYSNKILSIYHGDELNQAIQRCESSEQS

Sequences:

>Translated_291_residues
MTIQSIATSDFQGLNELKVQAKNNAKEALPEVAKQFEGIFLQSMLKSMRMGLHFLDESSPFSGKNEATFQEMLDTQYAST
IAESKGIGLAALLAKQLENSVGDKANNPVNSSTEVSNTKVTNSEESLSVVDDFVKSVWPTAKQAALLIGLDPKLLVAQAA
LETGWGKFVTRDADGSSSNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYPSIEHSFHDYISLIKGSERYQMA
LANAENPEIYVSELNKAGYATDPNYSNKILSIYHGDELNQAIQRCESSEQS
>Mature_290_residues
TIQSIATSDFQGLNELKVQAKNNAKEALPEVAKQFEGIFLQSMLKSMRMGLHFLDESSPFSGKNEATFQEMLDTQYASTI
AESKGIGLAALLAKQLENSVGDKANNPVNSSTEVSNTKVTNSEESLSVVDDFVKSVWPTAKQAALLIGLDPKLLVAQAAL
ETGWGKFVTRDADGSSSNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYPSIEHSFHDYISLIKGSERYQMAL
ANAENPEIYVSELNKAGYATDPNYSNKILSIYHGDELNQAIQRCESSEQS

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=284, Percent_Identity=32.0422535211268, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 31906; Mature: 31775

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQSIATSDFQGLNELKVQAKNNAKEALPEVAKQFEGIFLQSMLKSMRMGLHFLDESSP
CCCHHHCCHHHCCHHHHHHECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHECCCCCC
FSGKNEATFQEMLDTQYASTIAESKGIGLAALLAKQLENSVGDKANNPVNSSTEVSNTKV
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
TNSEESLSVVDDFVKSVWPTAKQAALLIGLDPKLLVAQAALETGWGKFVTRDADGSSSNN
CCCHHHHHHHHHHHHHHCCCCHHCEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCCCCC
LFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYPSIEHSFHDYISLIKGSERYQMA
EEEEECCCCCCHHHEEEEHHHHHCCCCEEECCCHHHCCCHHHHHHHHHHHHCCCHHHEEE
LANAENPEIYVSELNKAGYATDPNYSNKILSIYHGDELNQAIQRCESSEQS
ECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIQSIATSDFQGLNELKVQAKNNAKEALPEVAKQFEGIFLQSMLKSMRMGLHFLDESSP
CCHHHCCHHHCCHHHHHHECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHECCCCCC
FSGKNEATFQEMLDTQYASTIAESKGIGLAALLAKQLENSVGDKANNPVNSSTEVSNTKV
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
TNSEESLSVVDDFVKSVWPTAKQAALLIGLDPKLLVAQAALETGWGKFVTRDADGSSSNN
CCCHHHHHHHHHHHHHHCCCCHHCEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCCCCC
LFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYPSIEHSFHDYISLIKGSERYQMA
EEEEECCCCCCHHHEEEEHHHHHCCCCEEECCCHHHCCCHHHHHHHHHHHHCCCHHHEEE
LANAENPEIYVSELNKAGYATDPNYSNKILSIYHGDELNQAIQRCESSEQS
ECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]