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The map label for this gene is lig [H]

Identifier: 54293931

GI number: 54293931

Start: 1111323

End: 1113344

Strand: Direct

Name: lig [H]

Synonym: lpl0987

Alternate gene names: 54293931

Gene position: 1111323-1113344 (Clockwise)

Preceding gene: 54293926

Following gene: 54293932

Centisome position: 33.22

GC content: 42.24

Gene sequence:

>2022_bases
ATGAATGATCAAGGAATTAAGGAATCGATAGAAACGCTTAAAGAGCAAATAAGAAAATACGATTATCACTATTATGTTTT
AGATGAACCTTTGGTTCCCGACGCGGAATATGATCGATGTTTCAAGGCATTGCAACAGTATGAAGAGCAATATCCGCAAT
TTTTATCGCCGGACTCTCCTACACAGAGAGTGAGCGGTACTCCTTCAGATGCTTTTATGCCGGTAGCCCATAAGCAACCC
ATGTTGTCTTTGTCCAATGTGTTTACTACTGATGAATTAAAAGCATTCATTAAGCGAGCAATTGAGAAACTGGATGAACC
AAATCAACAACTGGTATTTGCTTGCGAACCAAAGCTTGATGGGTTGGCTGTTAACATGACTTATGAGGGCGGGATCTTGA
CTCATGCCGCAACTCGTGGCGATGGTGCTGTAGGTGAAAACATCACAGCAAATATTAAGACTATTGCTTCAGTTCCATTA
AGGCTAAGGGTTAGTAACCCTCCAAAATTGATCGAAGTGCGGGGTGAGGTCTATATCCCGAAAGCCGATTTTGAAGCATA
CAACGCAAGGGCTAGAGAACTCGGTGAAAAAACTTTTGCTAATCCGCGAAATGCTGCTGCAGGCAGTTTAAGGCAATTAA
ATCCTGAAATTTCTGCCAGTCGCCCACTTGCTATTTATTGTTATGGTATAGGGGCTTGCGAGGATTATAAGTTACCTAAC
AGTCATTTGGAGCAATTGAATTTATTAAAAGAATTTGGATTTAGAGTGTCTCCAGAAACGAGGAGGGCGGTTGGAATAGA
AGGCTGTTTAGATTATTACCAGTATATGTTAGCGAAACGGAATCAATTGCCATTTGAAATCGATGGGGTTGTTTATAAGA
TTGACAGTATCTCCTTGCAACAGCAATTAGGTTATGTTTCTCGTGCCCCAAGATTTGCTTGTGCCCATAAATTTCCCGCT
ACAGAAGAAATGACCCGTCTGATAGCCGTGGATTTCCAGGTAGGTAGAACGGGTGCTGTGACACCGGTTGCACGTTTGGA
GCCAGTTAGTGTTGGTGGCGTTACAGTAAGTAATGCGACTTTGCATAATTTTGATGAAATTACCCGAAAAGACATTCGTA
TTGGTGATACGGTTATTATTCGTCGTGCCGGTGATGTGATCCCTGAAGTGGTTTCTGTGATTTTGGAAAAGCGTCCCGCT
AATGCCAGAATGATTGAGCTTCCTAAAAATTGCCCTGTTTGTGGTTCTGAAGTCGTAAGGGAAGCCGATGAAGCAATTGC
TCGTTGTGTCGGCGGTTTATATTGCAAAGCACAATTAAAAAGGATGATGTGGCATTTTGCTTCTCGAAAAGCAATGTATA
TTGAAGGACTTGGCAGTGTTTTAATTGACCAGTTAGTTGATGAGGGTATTGTCCATCATTTGGCGGATCTTTATGAACTC
GACTTGCAGACTTTAGCTAGCCTGCCAAGGATGGGGGAGAAATCTGCAAAAAACCTATTATCCGCTTTGGAAAAAAGTAA
AAAAACGACTTTCAATCGCTTTCTTTATGCTTTGGGGATCAGAGAAATCGGTGAAGCCGGCGCAAGGGTTTTAGCGGAGC
ACTACTGCGATGTAGAGGGCTTGAAAGCGGCAACGATTGAGGAATTAATGACTCTGAATGACATAGGTCCAGTAGCGGCT
TCTCATGTAGTCCATTTCTTTGCCCAAGCGCATAATCTTGAAGTGATTGACCGTCTTCTCGAGTTGGGTATTCATTGGCC
TAAGTCCGAAAAAATACAGGTTAATCAGCAAAATCCATTTTTTGGTAAAACAGTAGTTTTAACTGGGACTCTGAGTACCA
TGGGGAGGGAAGAGGCAAAGGCAAAATTATTAGCCTTGGGCGCAAAAGTGAGTGGAAGTGTGTCTTCCAAAACGGATTAT
GTAGTAGCAGGAAGTGAAGCCGGTTCAAAACTGATTAAAGCGACAGAACTGGGAGTAGCGATTATAGAGGAAGACGAGTT
TTTAAAATGGGTTAATTCATGA

Upstream 100 bases:

>100_bases
GCTTCCAAAAAAAGATTTTGCCTTGAATGATTAAACCACTCATATTTGAAAGAAGTAAAAAAATGATAAACTGTTGTTTT
TATTTTGGGAATCTTCATTA

Downstream 100 bases:

>100_bases
GTAGCTAGATCTTTGTTATTTGGTTTATGATAGGTAGCAATGAATCGGGTGAAGTAGGTATTTAAACCTGTAATAATGAC
TGATTGGCACGAGGGAAGTA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSPTQRVSGTPSDAFMPVAHKQP
MLSLSNVFTTDELKAFIKRAIEKLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPL
RLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYGIGACEDYKLPN
SHLEQLNLLKEFGFRVSPETRRAVGIEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAPRFACAHKFPA
TEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPA
NARMIELPKNCPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGIVHHLADLYEL
DLQTLASLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVEGLKAATIEELMTLNDIGPVAA
SHVVHFFAQAHNLEVIDRLLELGIHWPKSEKIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLLALGAKVSGSVSSKTDY
VVAGSEAGSKLIKATELGVAIIEEDEFLKWVNS

Sequences:

>Translated_673_residues
MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSPTQRVSGTPSDAFMPVAHKQP
MLSLSNVFTTDELKAFIKRAIEKLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPL
RLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYGIGACEDYKLPN
SHLEQLNLLKEFGFRVSPETRRAVGIEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAPRFACAHKFPA
TEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPA
NARMIELPKNCPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGIVHHLADLYEL
DLQTLASLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVEGLKAATIEELMTLNDIGPVAA
SHVVHFFAQAHNLEVIDRLLELGIHWPKSEKIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLLALGAKVSGSVSSKTDY
VVAGSEAGSKLIKATELGVAIIEEDEFLKWVNS
>Mature_673_residues
MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSPTQRVSGTPSDAFMPVAHKQP
MLSLSNVFTTDELKAFIKRAIEKLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPL
RLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYGIGACEDYKLPN
SHLEQLNLLKEFGFRVSPETRRAVGIEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAPRFACAHKFPA
TEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPA
NARMIELPKNCPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGIVHHLADLYEL
DLQTLASLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVEGLKAATIEELMTLNDIGPVAA
SHVVHFFAQAHNLEVIDRLLELGIHWPKSEKIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLLALGAKVSGSVSSKTDY
VVAGSEAGSKLIKATELGVAIIEEDEFLKWVNS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=53.6512667660209, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=526, Percent_Identity=22.4334600760456, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74582; Mature: 74582

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSP
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCC
TQRVSGTPSDAFMPVAHKQPMLSLSNVFTTDELKAFIKRAIEKLDEPNQQLVFACEPKLD
CHHCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPLRLRVSNPPKLIEVRGEVYIP
CEEEEEEECCCEEEEECCCCCCCCCCCCEECHHHHCCCCEEEEECCCCCEEEECCCEEEC
KADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYGIGACEDYKLPN
CCCCHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCH
SHLEQLNLLKEFGFRVSPETRRAVGIEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCHHHH
QQLGYVSRAPRFACAHKFPATEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNAT
HHHHHHHCCCCHHHHCCCCCHHHHHHHHHEEEEECCCCCCCCHHHCCCEEECCEEECCCC
LHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPANARMIELPKNCPVCGSEVVR
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
EADEAIARCVGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGIVHHLADLYEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLQTLASLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVEG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LKAATIEELMTLNDIGPVAASHVVHFFAQAHNLEVIDRLLELGIHWPKSEKIQVNQQNPF
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECCCCCC
FGKTVVLTGTLSTMGREEAKAKLLALGAKVSGSVSSKTDYVVAGSEAGSKLIKATELGVA
CCCEEEEEECHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCEE
IIEEDEFLKWVNS
EECCHHHHHHHCC
>Mature Secondary Structure
MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSP
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCC
TQRVSGTPSDAFMPVAHKQPMLSLSNVFTTDELKAFIKRAIEKLDEPNQQLVFACEPKLD
CHHCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPLRLRVSNPPKLIEVRGEVYIP
CEEEEEEECCCEEEEECCCCCCCCCCCCEECHHHHCCCCEEEEECCCCCEEEECCCEEEC
KADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYGIGACEDYKLPN
CCCCHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCH
SHLEQLNLLKEFGFRVSPETRRAVGIEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCHHHH
QQLGYVSRAPRFACAHKFPATEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNAT
HHHHHHHCCCCHHHHCCCCCHHHHHHHHHEEEEECCCCCCCCHHHCCCEEECCEEECCCC
LHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPANARMIELPKNCPVCGSEVVR
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
EADEAIARCVGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGIVHHLADLYEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLQTLASLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVEG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LKAATIEELMTLNDIGPVAASHVVHFFAQAHNLEVIDRLLELGIHWPKSEKIQVNQQNPF
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECCCCCC
FGKTVVLTGTLSTMGREEAKAKLLALGAKVSGSVSSKTDYVVAGSEAGSKLIKATELGVA
CCCEEEEEECHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCEE
IIEEDEFLKWVNS
EECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA