Click here to switch to the map view.

The map label for this gene is metB [H]

Identifier: 54293865

GI number: 54293865

Start: 1032898

End: 1034049

Strand: Reverse

Name: metB [H]

Synonym: lpl0921

Alternate gene names: 54293865

Gene position: 1034049-1032898 (Counterclockwise)

Preceding gene: 54293872

Following gene: 54293864

Centisome position: 30.91

GC content: 41.93

Gene sequence:

>1152_bases
ATGAATAAGACTCATTTCGATACACGTGCAATTCATGCAGGACAAGAACCATGTCAAAGTACAGGGGCCGTGATGACCCC
TATTTATGCTACCTCCACCTATAAGCAAATTGCCCCTGGCGAACATCTTGGCTATGAATACTCACGTACCCAGAACCCCA
CCAGAAAAGCCTATGAGGATTGCATAGCAAGCCTTGAATCAGGCCAAAAAGGATTTGCGTTTGCTTCGGGCATGGCTGCA
ATCAATACAGTTATTGATTTATTAGATTCCGGAGATCACGTCGTTGCCATGGATGATCTTTATGGAGGAACTTTCCGGCT
TTTTGATAAAGTAAAAACCCGAACTTCTAATTTATCCTTTTCTTTTGTTGATATGTCAGTACCCGAGAATATTGAAGCTG
CAATAACTCCGAAGACAAAATTACTATGGTTGGAAACCCCTTCCAATCCCATGCTAAAGCTAGCTAATTTACGAAAAATC
GCTGCTATTGCAAAAAAACATAATTTGATTACAGTTGCCGATAACACCTTTGCAACTCCCTGGATTCAACGCCCTCTTGA
GCTAGGATTTGACATTGTACTTCATTCAGCAACCAAATATCTGAACGGACATTCTGATGTAATCAGTGGTGTCGTTGTCG
TCGGTGACAATCCCATGTTGTCTGATAAAATTGCTTTTTTGCAAAATTCATGCGGCGCTGTAGCTGGCCCTTTCGACAGT
TTCCTGGTTCTGAGAAGCTTAAAAACATTATCTCTTCGTATGCAAAGGCACTGTGAAAATGCCAACCATCTGGCTAACTG
GCTTAGCAGCCACCCCAAGATTGAAAAAGTCATTTATCCCGGATTAAAAAGTCATCCGCAATATTCTCTTGCCAAGGAAC
AAATGAACGATTTCGGTGGTATGATCTCTTTGGTACTTAAAGGTGGTCTTGAAGATGCCAAACGATTTTTAGCTCGCTGC
GAATTATTTACTTTGGCTGAAAGCCTTGGGGGTGTAGAAAGTTTAATTGAACACCCTGCAATTATGACCCATGCCTCTAT
TCCAGTCGAACAACGCAAAGCCCTGGGCATAGAAGATGGTTTCATAAGATTATCAGTTGGTATTGAACACATTGATGATC
TGCGTGCCGATCTGGAGCATGCTTTAGGTTAA

Upstream 100 bases:

>100_bases
GAACTTTAGCAAAAAAGGCGCCGAAAGTATAATTTTTAATCAGTATTTTGATCACCTATTGGTATTTCTTTGAATTTAAA
TATTTTTATGGGAATAAAAA

Downstream 100 bases:

>100_bases
AAGATGTAAATCCTGGTTCTGACTGTTAAAAAGAACCAGGGCAGATGTGTATAGGAGAAGTATTTCATGCAAAAAAATAA
ACAAAATGGCGGGCAGCTCC

Product: hypothetical protein

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNKTHFDTRAIHAGQEPCQSTGAVMTPIYATSTYKQIAPGEHLGYEYSRTQNPTRKAYEDCIASLESGQKGFAFASGMAA
INTVIDLLDSGDHVVAMDDLYGGTFRLFDKVKTRTSNLSFSFVDMSVPENIEAAITPKTKLLWLETPSNPMLKLANLRKI
AAIAKKHNLITVADNTFATPWIQRPLELGFDIVLHSATKYLNGHSDVISGVVVVGDNPMLSDKIAFLQNSCGAVAGPFDS
FLVLRSLKTLSLRMQRHCENANHLANWLSSHPKIEKVIYPGLKSHPQYSLAKEQMNDFGGMISLVLKGGLEDAKRFLARC
ELFTLAESLGGVESLIEHPAIMTHASIPVEQRKALGIEDGFIRLSVGIEHIDDLRADLEHALG

Sequences:

>Translated_383_residues
MNKTHFDTRAIHAGQEPCQSTGAVMTPIYATSTYKQIAPGEHLGYEYSRTQNPTRKAYEDCIASLESGQKGFAFASGMAA
INTVIDLLDSGDHVVAMDDLYGGTFRLFDKVKTRTSNLSFSFVDMSVPENIEAAITPKTKLLWLETPSNPMLKLANLRKI
AAIAKKHNLITVADNTFATPWIQRPLELGFDIVLHSATKYLNGHSDVISGVVVVGDNPMLSDKIAFLQNSCGAVAGPFDS
FLVLRSLKTLSLRMQRHCENANHLANWLSSHPKIEKVIYPGLKSHPQYSLAKEQMNDFGGMISLVLKGGLEDAKRFLARC
ELFTLAESLGGVESLIEHPAIMTHASIPVEQRKALGIEDGFIRLSVGIEHIDDLRADLEHALG
>Mature_383_residues
MNKTHFDTRAIHAGQEPCQSTGAVMTPIYATSTYKQIAPGEHLGYEYSRTQNPTRKAYEDCIASLESGQKGFAFASGMAA
INTVIDLLDSGDHVVAMDDLYGGTFRLFDKVKTRTSNLSFSFVDMSVPENIEAAITPKTKLLWLETPSNPMLKLANLRKI
AAIAKKHNLITVADNTFATPWIQRPLELGFDIVLHSATKYLNGHSDVISGVVVVGDNPMLSDKIAFLQNSCGAVAGPFDS
FLVLRSLKTLSLRMQRHCENANHLANWLSSHPKIEKVIYPGLKSHPQYSLAKEQMNDFGGMISLVLKGGLEDAKRFLARC
ELFTLAESLGGVESLIEHPAIMTHASIPVEQRKALGIEDGFIRLSVGIEHIDDLRADLEHALG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=365, Percent_Identity=52.6027397260274, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI299473759, Length=365, Percent_Identity=47.6712328767123, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI25453487, Length=364, Percent_Identity=47.2527472527472, Blast_Score=309, Evalue=3e-84,
Organism=Escherichia coli, GI1790375, Length=376, Percent_Identity=42.2872340425532, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI1789383, Length=398, Percent_Identity=27.1356783919598, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17538055, Length=368, Percent_Identity=49.4565217391304, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17560056, Length=368, Percent_Identity=48.0978260869565, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI71980708, Length=391, Percent_Identity=37.8516624040921, Blast_Score=257, Evalue=6e-69,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=47.012987012987, Blast_Score=345, Evalue=6e-96,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=34.4748858447489, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=404, Percent_Identity=33.9108910891089, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321170, Length=339, Percent_Identity=28.3185840707965, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321904, Length=343, Percent_Identity=30.3206997084548, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI19922554, Length=380, Percent_Identity=49.2105263157895, Blast_Score=382, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 42035; Mature: 42035

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTHFDTRAIHAGQEPCQSTGAVMTPIYATSTYKQIAPGEHLGYEYSRTQNPTRKAYED
CCCCCCCHHHHHCCCCHHHHCCCEECCEEECCCHHHCCCCCCCCCCHHCCCCHHHHHHHH
CIASLESGQKGFAFASGMAAINTVIDLLDSGDHVVAMDDLYGGTFRLFDKVKTRTSNLSF
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEE
SFVDMSVPENIEAAITPKTKLLWLETPSNPMLKLANLRKIAAIAKKHNLITVADNTFATP
EEEECCCCCCCCEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCH
WIQRPLELGFDIVLHSATKYLNGHSDVISGVVVVGDNPMLSDKIAFLQNSCGAVAGPFDS
HHHHHHHCCHHHHHHHHHHHHCCCHHHHCCEEEECCCCCCHHHHHHHHHCCCCCCCCHHH
FLVLRSLKTLSLRMQRHCENANHLANWLSSHPKIEKVIYPGLKSHPQYSLAKEQMNDFGG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHH
MISLVLKGGLEDAKRFLARCELFTLAESLGGVESLIEHPAIMTHASIPVEQRKALGIEDG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEECCCCHHHHHHCCCCCC
FIRLSVGIEHIDDLRADLEHALG
EEEEEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKTHFDTRAIHAGQEPCQSTGAVMTPIYATSTYKQIAPGEHLGYEYSRTQNPTRKAYED
CCCCCCCHHHHHCCCCHHHHCCCEECCEEECCCHHHCCCCCCCCCCHHCCCCHHHHHHHH
CIASLESGQKGFAFASGMAAINTVIDLLDSGDHVVAMDDLYGGTFRLFDKVKTRTSNLSF
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEE
SFVDMSVPENIEAAITPKTKLLWLETPSNPMLKLANLRKIAAIAKKHNLITVADNTFATP
EEEECCCCCCCCEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCH
WIQRPLELGFDIVLHSATKYLNGHSDVISGVVVVGDNPMLSDKIAFLQNSCGAVAGPFDS
HHHHHHHCCHHHHHHHHHHHHCCCHHHHCCEEEECCCCCCHHHHHHHHHCCCCCCCCHHH
FLVLRSLKTLSLRMQRHCENANHLANWLSSHPKIEKVIYPGLKSHPQYSLAKEQMNDFGG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHH
MISLVLKGGLEDAKRFLARCELFTLAESLGGVESLIEHPAIMTHASIPVEQRKALGIEDG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEECCCCHHHHHHCCCCCC
FIRLSVGIEHIDDLRADLEHALG
EEEEEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]