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The map label for this gene is mlaE [H]

Identifier: 54293817

GI number: 54293817

Start: 988920

End: 989702

Strand: Direct

Name: mlaE [H]

Synonym: lpl0873

Alternate gene names: 54293817

Gene position: 988920-989702 (Clockwise)

Preceding gene: 54293816

Following gene: 54293818

Centisome position: 29.56

GC content: 39.97

Gene sequence:

>783_bases
ATGCTTGATACAATCGCGCGTCTGGGTAATCGAGGTGTACAAGCCTTACAAGGTATTGGTAGTTCAGGGCTGTTTTTATT
TTCCATGTTGTTCAGAAAGCCTAATGTTTTAAGGTTATGGCCATTATTACGTTATCAGCTTTATTTTATCGGTGTATTGT
CTTGTTTAATTATTGTGGTTTCAGCCTTGTTTATTGGTATGGTTGTAGGACTTCAGGGGTATAATACGTTACAAAAATTT
GGTGCTTCAAGTCAATTAGGTCAATTATTGGCTTTGAGCGTCGCCAGAGAATTAGGACCTGTGATTAGCGCTCTGTTGTT
TGCAGGGCGTGCCGGGTCGGCTCTTACAGCTGAAATTGGTTTAATGAAAGCAACAGAGCAGTTATCCAGTATGGATATGA
TGGGAGTGGATCCATTAGGAAGAGTGGTTTATCCGCGTTTTGTGGCAGGATTTATAGCGTTACCGATACTTGCTTTGATT
TTTTCTGCAGTAGCTATATATGGCGGTTATTTTATTGGTGTTTACTGGTTAGGTGTGGATGCAGGGAGTTTTTGGTCTAA
TATGCAAGCTGCTGTAAATTTTCGTATAGATATCTTAAGCGGGATTATTAAGAGCCTGGTTTTCGCATTTGTGGTTACCT
GGATTTCTGTATACCAAGGTTTTGAGTGTGTGCCAACAGCTGAAGGCATCAGTCATGCTACAACTAAAACTGTAGTATAC
TCATCGCTTGCTGTATTAGGTCTTGATTTCTTATTGACTGCAATGATGATTGGAGATTGGTAA

Upstream 100 bases:

>100_bases
CGATCTATTGAGCCTCAGATAAGACAGTTTATGCATGGAGAAGCGGATGGGGTAGTGCCTTTCCATTATCCGGCAAAACC
GTATACAGAGGAGTTATTAA

Downstream 100 bases:

>100_bases
ATGAAAAAGCAACGTTATGTGGACATTAGTGTAGGAATATTTATGTTGCTTGGGCTGCTAGCATTATTAGTAATGGCGAT
GAAAGTGAGTAACATTACTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLDTIARLGNRGVQALQGIGSSGLFLFSMLFRKPNVLRLWPLLRYQLYFIGVLSCLIIVVSALFIGMVVGLQGYNTLQKF
GASSQLGQLLALSVARELGPVISALLFAGRAGSALTAEIGLMKATEQLSSMDMMGVDPLGRVVYPRFVAGFIALPILALI
FSAVAIYGGYFIGVYWLGVDAGSFWSNMQAAVNFRIDILSGIIKSLVFAFVVTWISVYQGFECVPTAEGISHATTKTVVY
SSLAVLGLDFLLTAMMIGDW

Sequences:

>Translated_260_residues
MLDTIARLGNRGVQALQGIGSSGLFLFSMLFRKPNVLRLWPLLRYQLYFIGVLSCLIIVVSALFIGMVVGLQGYNTLQKF
GASSQLGQLLALSVARELGPVISALLFAGRAGSALTAEIGLMKATEQLSSMDMMGVDPLGRVVYPRFVAGFIALPILALI
FSAVAIYGGYFIGVYWLGVDAGSFWSNMQAAVNFRIDILSGIIKSLVFAFVVTWISVYQGFECVPTAEGISHATTKTVVY
SSLAVLGLDFLLTAMMIGDW
>Mature_260_residues
MLDTIARLGNRGVQALQGIGSSGLFLFSMLFRKPNVLRLWPLLRYQLYFIGVLSCLIIVVSALFIGMVVGLQGYNTLQKF
GASSQLGQLLALSVARELGPVISALLFAGRAGSALTAEIGLMKATEQLSSMDMMGVDPLGRVVYPRFVAGFIALPILALI
FSAVAIYGGYFIGVYWLGVDAGSFWSNMQAAVNFRIDILSGIIKSLVFAFVVTWISVYQGFECVPTAEGISHATTKTVVY
SSLAVLGLDFLLTAMMIGDW

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=61.003861003861, Blast_Score=315, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28017; Mature: 28017

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]