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The map label for this gene is rluE [H]

Identifier: 54293791

GI number: 54293791

Start: 962437

End: 963039

Strand: Reverse

Name: rluE [H]

Synonym: lpl0847

Alternate gene names: 54293791

Gene position: 963039-962437 (Counterclockwise)

Preceding gene: 54293798

Following gene: 54293781

Centisome position: 28.78

GC content: 38.97

Gene sequence:

>603_bases
ATGCCGCAATTAATACTTTTTAATAAACCTTATGGAGTCTTGACTCAATTTACTGGCGAATTACCCGAACAAACTCTCTC
GGCATTTATTAAATTTCCGGGTTTTTATGCGGCTGGACGTCTGGATAAACAAAGTGAGGGCTTACTTATCTTAACTGATG
ATGGAAAACTACAACACCGGTTAACCCATCCTAAATTTGAGAAGAAGAAACATTACTGGGTTCAAGTAGAGGGGGTCCCT
ACAGACAAAGATTTACAACCTTTAAGAAAAGGCCTGGTAATCAAAGACATTACTTTTTTACCCGCCGAAGCCAAGTTGAT
TTCAGAGCCAAAAATCTGGCCACGAATTCCGCCCATAAGAGAAAGGAAACACATACCAACTACCTGGCTGGGAATTATTT
TAAGAGAAGGAAAAAATCATCAAATAAGAAAAATGACAGCCGCTATTGGATTTCCTACTTTACGTTTAATTAGATACCAG
ATTGGTGATTGGCACTTAGATCAACTCCAGCCCGGGGAATATAAATTAATCAACCTTACTAACGATAAACTAAACGAGTT
AACAAGGAAGAGTAACCCAAGACCCGAATCCAGGGCCAAATAA

Upstream 100 bases:

>100_bases
CGCCGTTTATTGTGCTTAAAGGCGTGATCTGGTCCTGAAGTGGTTGCTGACAATAACCAAAAAATTTATCATTAATTTCC
ACAATCAACGCAATCATAAA

Downstream 100 bases:

>100_bases
TCATACCCAAAATGGCACTTGTCACGGGCATTATCAGGTTATAATTAAAACCTAACAGAGCATCAATAAATATAATAATT
GATTGATATAGAAAACAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQSEGLLILTDDGKLQHRLTHPKFEKKKHYWVQVEGVP
TDKDLQPLRKGLVIKDITFLPAEAKLISEPKIWPRIPPIRERKHIPTTWLGIILREGKNHQIRKMTAAIGFPTLRLIRYQ
IGDWHLDQLQPGEYKLINLTNDKLNELTRKSNPRPESRAK

Sequences:

>Translated_200_residues
MPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQSEGLLILTDDGKLQHRLTHPKFEKKKHYWVQVEGVP
TDKDLQPLRKGLVIKDITFLPAEAKLISEPKIWPRIPPIRERKHIPTTWLGIILREGKNHQIRKMTAAIGFPTLRLIRYQ
IGDWHLDQLQPGEYKLINLTNDKLNELTRKSNPRPESRAK
>Mature_199_residues
PQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQSEGLLILTDDGKLQHRLTHPKFEKKKHYWVQVEGVPT
DKDLQPLRKGLVIKDITFLPAEAKLISEPKIWPRIPPIRERKHIPTTWLGIILREGKNHQIRKMTAAIGFPTLRLIRYQI
GDWHLDQLQPGEYKLINLTNDKLNELTRKSNPRPESRAK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=57.9545454545455, Blast_Score=207, Evalue=4e-55,
Organism=Escherichia coli, GI1788510, Length=178, Percent_Identity=31.4606741573034, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1787524, Length=168, Percent_Identity=31.547619047619, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 23187; Mature: 23056

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQSEGLLILTDDGKLQHR
CCCEEEEECCCHHHHHHCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCEEEC
LTHPKFEKKKHYWVQVEGVPTDKDLQPLRKGLVIKDITFLPAEAKLISEPKIWPRIPPIR
CCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCEEEEEEECCCCHHCCCCCCCCCCCCCCC
ERKHIPTTWLGIILREGKNHQIRKMTAAIGFPTLRLIRYQIGDWHLDQLQPGEYKLINLT
CCCCCCHHHEEEEEECCCCCHHHHHHHHHCCCHHHHEEHHCCCEECCCCCCCCEEEEEEC
NDKLNELTRKSNPRPESRAK
CHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQSEGLLILTDDGKLQHR
CCEEEEECCCHHHHHHCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCEEEC
LTHPKFEKKKHYWVQVEGVPTDKDLQPLRKGLVIKDITFLPAEAKLISEPKIWPRIPPIR
CCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCEEEEEEECCCCHHCCCCCCCCCCCCCCC
ERKHIPTTWLGIILREGKNHQIRKMTAAIGFPTLRLIRYQIGDWHLDQLQPGEYKLINLT
CCCCCCHHHEEEEEECCCCCHHHHHHHHHCCCHHHHEEHHCCCEECCCCCCCCEEEEEEC
NDKLNELTRKSNPRPESRAK
CHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]