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The map label for this gene is irlS [H]

Identifier: 54293698

GI number: 54293698

Start: 852655

End: 853728

Strand: Reverse

Name: irlS [H]

Synonym: lpl0751

Alternate gene names: 54293698

Gene position: 853728-852655 (Counterclockwise)

Preceding gene: 54293699

Following gene: 54293693

Centisome position: 25.52

GC content: 34.17

Gene sequence:

>1074_bases
ATGTATTGGAGATTCGCTAAATCAATGTCTATTACTAACCGCTTATTACTTATATTTATTGGTTCAACCGTACTGATATT
GTCTATTATCACAGGGTTAATCTATCCTCCAATGAAAGAATTACTCCATCATACTCATTTGAATAATGAGCATTATAATT
ATTTATTAACACGAATTTGTATTAAAAATTTCTTTGTTGGACTCTGGTTTTCTACTTTGATAATCATTCTTGCCAGTTAT
TTGCTCGCAAAAAAAAGCATGAGACCAATTAAAATATTCACTAAAGAACTTGCATCGATTAATGCTTCATCACTAGAGAA
ACGATTACCTTACACAGGGAATCCCAAAGAACTGGAAGAATTAGCCTCGACTTGCAATGACATGCTCTTTCGAATAGAAA
GTGCATTTAGTCATATAAAGCAGTTTTCAGCCAGTATAGCTCATGAATTACGAAATCCCATTCATTACCTCAGAACTGCC
ACCGAAATTACCCTGACTAAACCACAAACGATTGAAACCTATCAACATGTATTACAAACGCATCTCGAAGAATATCAAAG
TTTAACCCAACTTATAGATAATCTGCTCTTTTTAACTCGCTGTGAGCACGGCCAACTTCAATTAAATTTAAGAACATTAT
CTGCAAACCACTTAATCAGTTCAATTATTGAGTATTATTATTCAATAGCTCAAGAAAAGGGCATAGAAATTCAAGTTTTT
GGTGAGGCTCAGATTGAAGTAGATGAACATTTATTTAAGCGCGTTATTTCAAATTTAATAGACAACAGTCTGACCTATAC
TAACAAAGGCGGAAGAATACTAATCATCATAGAAAAAAGAACAGACGACAGCATTCAAATCCGTATAAAAGACAATGGAA
TTGGTATATCAAAAGAACACTTACCTCTTTTATATCACGGATTTTATCGAGTAGATTATAGTACTAACAAAGAAAACGCA
GGCCTTGGTTTAGGTTTGGCCATTGCTAAATCAATCATGGAGCACCATAAAGGCCAGCTTGCGGTAGATAGCAAGATAGG
TTTAGGCACCACTGTTATATTAACCCTACCATGA

Upstream 100 bases:

>100_bases
ACGAATACCAATATTATTGATGTAGCGATCAAGCGTTTAAGGGAAAAAGTGGATAATGATCATCCTACTAAATTGATCCA
TACTGTCAGAGGAGTAGGCT

Downstream 100 bases:

>100_bases
AAAAGCTTTTTAAAATTGAATAATATATGTATTTCATCTATCCGAAACCATAAGGGATCGGATATTGAACCAAGTTATTC
TAAGAACATAGAGTTTTCCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MYWRFAKSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHHTHLNNEHYNYLLTRICIKNFFVGLWFSTLIIILASY
LLAKKSMRPIKIFTKELASINASSLEKRLPYTGNPKELEELASTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTA
TEITLTKPQTIETYQHVLQTHLEEYQSLTQLIDNLLFLTRCEHGQLQLNLRTLSANHLISSIIEYYYSIAQEKGIEIQVF
GEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILIIIEKRTDDSIQIRIKDNGIGISKEHLPLLYHGFYRVDYSTNKENA
GLGLGLAIAKSIMEHHKGQLAVDSKIGLGTTVILTLP

Sequences:

>Translated_357_residues
MYWRFAKSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHHTHLNNEHYNYLLTRICIKNFFVGLWFSTLIIILASY
LLAKKSMRPIKIFTKELASINASSLEKRLPYTGNPKELEELASTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTA
TEITLTKPQTIETYQHVLQTHLEEYQSLTQLIDNLLFLTRCEHGQLQLNLRTLSANHLISSIIEYYYSIAQEKGIEIQVF
GEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILIIIEKRTDDSIQIRIKDNGIGISKEHLPLLYHGFYRVDYSTNKENA
GLGLGLAIAKSIMEHHKGQLAVDSKIGLGTTVILTLP
>Mature_357_residues
MYWRFAKSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHHTHLNNEHYNYLLTRICIKNFFVGLWFSTLIIILASY
LLAKKSMRPIKIFTKELASINASSLEKRLPYTGNPKELEELASTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTA
TEITLTKPQTIETYQHVLQTHLEEYQSLTQLIDNLLFLTRCEHGQLQLNLRTLSANHLISSIIEYYYSIAQEKGIEIQVF
GEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILIIIEKRTDDSIQIRIKDNGIGISKEHLPLLYHGFYRVDYSTNKENA
GLGLGLAIAKSIMEHHKGQLAVDSKIGLGTTVILTLP

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=287, Percent_Identity=34.1463414634146, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1788279, Length=260, Percent_Identity=30.7692307692308, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786600, Length=283, Percent_Identity=27.5618374558304, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788393, Length=277, Percent_Identity=28.5198555956679, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=26.8292682926829, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790346, Length=277, Percent_Identity=26.7148014440433, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=30.2631578947368, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=26.2008733624454, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=29.2576419213974, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790551, Length=358, Percent_Identity=23.7430167597765, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789403, Length=264, Percent_Identity=26.5151515151515, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=29.0697674418605, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=23.4782608695652, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=26.3636363636364, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787894, Length=243, Percent_Identity=25.5144032921811, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790861, Length=219, Percent_Identity=25.5707762557078, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787374, Length=294, Percent_Identity=23.1292517006803, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081816, Length=248, Percent_Identity=22.5806451612903, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40826; Mature: 40826

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYWRFAKSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHHTHLNNEHYNYLLTRIC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHH
IKNFFVGLWFSTLIIILASYLLAKKSMRPIKIFTKELASINASSLEKRLPYTGNPKELEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHH
LASTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTATEITLTKPQTIETYQHVLQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHH
HLEEYQSLTQLIDNLLFLTRCEHGQLQLNLRTLSANHLISSIIEYYYSIAQEKGIEIQVF
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEE
GEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILIIIEKRTDDSIQIRIKDNGIGISKEH
CCCEEHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEECCCCEEEEEEECCCCCCCHHC
LPLLYHGFYRVDYSTNKENAGLGLGLAIAKSIMEHHKGQLAVDSKIGLGTTVILTLP
CCCEEECEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECC
>Mature Secondary Structure
MYWRFAKSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHHTHLNNEHYNYLLTRIC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHH
IKNFFVGLWFSTLIIILASYLLAKKSMRPIKIFTKELASINASSLEKRLPYTGNPKELEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHH
LASTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTATEITLTKPQTIETYQHVLQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHH
HLEEYQSLTQLIDNLLFLTRCEHGQLQLNLRTLSANHLISSIIEYYYSIAQEKGIEIQVF
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEE
GEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILIIIEKRTDDSIQIRIKDNGIGISKEH
CCCEEHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEECCCCEEEEEEECCCCCCCHHC
LPLLYHGFYRVDYSTNKENAGLGLGLAIAKSIMEHHKGQLAVDSKIGLGTTVILTLP
CCCEEECEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]