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The map label for this gene is slt [H]

Identifier: 54293647

GI number: 54293647

Start: 786945

End: 788726

Strand: Reverse

Name: slt [H]

Synonym: lpl0700

Alternate gene names: 54293647

Gene position: 788726-786945 (Counterclockwise)

Preceding gene: 54293648

Following gene: 54293640

Centisome position: 23.57

GC content: 37.21

Gene sequence:

>1782_bases
ATGAAAAAATTATTAATTTTTTTATGTGGGCTTCTCTGTTCTTTCAACTTGTTTGCTTTATCTGGACAAGCCTACATGGA
CAGGTTTAATCGCTATCTTGCATGGTATGAACAGCTTCCTATTAACCCATCGCCAGAATTTTTGGAATTTGTAAAAGAAA
ACACACCGCTTGCTAATAAATTGCGTAACAAATGGTTATATGAACTGGCCAGGATAAAGGATTGGGAAGATTATAATAAG
TATTATCAACCTTCCAACGACATAAACCTCAGATGCTTTGCGCAAATTGCGAAATTCAATTTAGGATTATACAAAGAGGC
TATAGCAGGCTCTATTCCAATCTGGTTATCAGGAGAATCAAGACCCCAGGCATGCAATACCCTATTTGCATTACTGTTAA
AGCAGGATAACTTTGATCAGAAATTCATTACGCAACGCATAGCACTTGCACTAGACAAACGTAATGTTCTACTTGCTCGT
TACCTGTTAAAACAATACAAAACACCTCACGATACAGAAATAAAAAACTTAACCAGTGTTTATGAGAATCCTTCCAATAT
TTCCAAGTTGAACCCTGGCAAATTGAACGATTATTTCTATCTTTATGGATTAAAAAGATTAGTATCCATTAACATGGATA
AAGCAGTGAAACTATGGCAAGAGAAAAAAACCAACATCATGTTAAATGAGCGGCAAAAACAAGATTTTCTTGCACATTTG
GCATTATACAAGGCCATGAGAAATCATGCCGATGCTCAGGAATGGTTTGCCAAAATAAAACCTGAATACTACACCGATGT
GTTAGTCGACTGGCAAATCCGCTTTGCTCTGAAAAATAAAAACTGGAAACAAGTTGAGAAACTAATTAACAACTCACCAA
ATAAAGAGATGCCTTGTTGGCAATATTGGTTGGCTCGCTCCCTGGAAGAACAAGGGAAAAAAGCAGAGGCGATTAAAATT
TATGAGCCACTGGCTAAAAACAGGCATTATTATGGTTTTCTAGCCAGCAAACGCTTGAAAAAAAGACCCAGCTTTGACAA
TGAAAAACCAACAACCAACACAGAGATTCTCAAACCCTATCAACCTGTCATAGAGCAAGTTAAAAATTTTTACAACTCCA
AACAGCTACTGCAGGCATCAAGGTTATTAAATGACTTCATTAGTGAATTACCCAAAGAAGAGGCAAGTGCTCTGGTCTAT
TGGATAGCAACCGAACTAAAGTGGCATGGAAAATCAGTTTACTTAAGCAATAATGACACACTCAATAATCAATTAGCATT
AAGATTTCCATTGGCCTACAAAGACACAATAAAAGTATATGCGTCAAAATACGCCATACCTCCTGAGCTTGTATATGCTA
TTATTCGTCAGGAAAGTGGTTTTAGAGAAGATGTAACATCCTCTGCTGGGGCTAAAGGACTAATGCAAGTCATGCCACAA
ACAGCGAGCGCTGTTTCCAAAACCAGCAAAATACCGTATTCGGATCATAAGCAATTGTTTTTATCTCAAAAGAATATCAA
TATAGGGATTGCTTATTTACAGCACTTAACCAAGCGCTTCAGCAACCACCCGGTTCTTGTTGCTGCCGCTTACAACGCTG
GTCCCAGACAAGTAGTCTATTGGCTTAAAAATCACCCTCCCAAAGAAATAGATATCTGGATTGAAACACTTCCCTGGCAA
GAAACACGAAATTATCTAAAAAATGTAATAGCCTTTTATATTGTTTATCAGTACCGTTTAAACCAACAGCCTGATATGAA
TAGGTTTCTAACACCTTTGTAA

Upstream 100 bases:

>100_bases
ATAGCAGTAATTATAGTGAAACCATTAAACATATGCGTGAACAATTAGCAAGCATTTAAAAGTGCCAAATAATTGCCCTC
AAGCCTTATTAAGAGGAATT

Downstream 100 bases:

>100_bases
GAGATATTTTCATGGTAGTAAACGTAAATCGTTCTATGGCCGGATCTACGTTTGCGAGATACCTGTCTGACTTTTGCTAA
TTTAACCTAATGGGCGAGAG

Product: hypothetical protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MKKLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEQLPINPSPEFLEFVKENTPLANKLRNKWLYELARIKDWEDYNK
YYQPSNDINLRCFAQIAKFNLGLYKEAIAGSIPIWLSGESRPQACNTLFALLLKQDNFDQKFITQRIALALDKRNVLLAR
YLLKQYKTPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKLWQEKKTNIMLNERQKQDFLAHL
ALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFALKNKNWKQVEKLINNSPNKEMPCWQYWLARSLEEQGKKAEAIKI
YEPLAKNRHYYGFLASKRLKKRPSFDNEKPTTNTEILKPYQPVIEQVKNFYNSKQLLQASRLLNDFISELPKEEASALVY
WIATELKWHGKSVYLSNNDTLNNQLALRFPLAYKDTIKVYASKYAIPPELVYAIIRQESGFREDVTSSAGAKGLMQVMPQ
TASAVSKTSKIPYSDHKQLFLSQKNINIGIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVYWLKNHPPKEIDIWIETLPWQ
ETRNYLKNVIAFYIVYQYRLNQQPDMNRFLTPL

Sequences:

>Translated_593_residues
MKKLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEQLPINPSPEFLEFVKENTPLANKLRNKWLYELARIKDWEDYNK
YYQPSNDINLRCFAQIAKFNLGLYKEAIAGSIPIWLSGESRPQACNTLFALLLKQDNFDQKFITQRIALALDKRNVLLAR
YLLKQYKTPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKLWQEKKTNIMLNERQKQDFLAHL
ALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFALKNKNWKQVEKLINNSPNKEMPCWQYWLARSLEEQGKKAEAIKI
YEPLAKNRHYYGFLASKRLKKRPSFDNEKPTTNTEILKPYQPVIEQVKNFYNSKQLLQASRLLNDFISELPKEEASALVY
WIATELKWHGKSVYLSNNDTLNNQLALRFPLAYKDTIKVYASKYAIPPELVYAIIRQESGFREDVTSSAGAKGLMQVMPQ
TASAVSKTSKIPYSDHKQLFLSQKNINIGIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVYWLKNHPPKEIDIWIETLPWQ
ETRNYLKNVIAFYIVYQYRLNQQPDMNRFLTPL
>Mature_593_residues
MKKLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEQLPINPSPEFLEFVKENTPLANKLRNKWLYELARIKDWEDYNK
YYQPSNDINLRCFAQIAKFNLGLYKEAIAGSIPIWLSGESRPQACNTLFALLLKQDNFDQKFITQRIALALDKRNVLLAR
YLLKQYKTPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKLWQEKKTNIMLNERQKQDFLAHL
ALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFALKNKNWKQVEKLINNSPNKEMPCWQYWLARSLEEQGKKAEAIKI
YEPLAKNRHYYGFLASKRLKKRPSFDNEKPTTNTEILKPYQPVIEQVKNFYNSKQLLQASRLLNDFISELPKEEASALVY
WIATELKWHGKSVYLSNNDTLNNQLALRFPLAYKDTIKVYASKYAIPPELVYAIIRQESGFREDVTSSAGAKGLMQVMPQ
TASAVSKTSKIPYSDHKQLFLSQKNINIGIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVYWLKNHPPKEIDIWIETLPWQ
ETRNYLKNVIAFYIVYQYRLNQQPDMNRFLTPL

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=563, Percent_Identity=30.0177619893428, Blast_Score=214, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 69669; Mature: 69669

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEQLPINPSPEFLEFVKENTPLANK
CHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHH
LRNKWLYELARIKDWEDYNKYYQPSNDINLRCFAQIAKFNLGLYKEAIAGSIPIWLSGES
HHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
RPQACNTLFALLLKQDNFDQKFITQRIALALDKRNVLLARYLLKQYKTPHDTEIKNLTSV
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
YENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKLWQEKKTNIMLNERQKQDFLAHL
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECHHHHHHHHHHH
ALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFALKNKNWKQVEKLINNSPNKEMPCW
HHHHHHHCCCCHHHHHHHCCCCHHHHEEEEEEEEEEECCCCHHHHHHHHCCCCCCCCCHH
QYWLARSLEEQGKKAEAIKIYEPLAKNRHYYGFLASKRLKKRPSFDNEKPTTNTEILKPY
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHCCCCCCCCCCCCCCHHCCCH
QPVIEQVKNFYNSKQLLQASRLLNDFISELPKEEASALVYWIATELKWHGKSVYLSNNDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEEHHEECCCEEEECCCCC
LNNQLALRFPLAYKDTIKVYASKYAIPPELVYAIIRQESGFREDVTSSAGAKGLMQVMPQ
CCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHH
TASAVSKTSKIPYSDHKQLFLSQKNINIGIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVY
HHHHHHHHCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHEEE
WLKNHPPKEIDIWIETLPWQETRNYLKNVIAFYIVYQYRLNQQPDMNRFLTPL
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCC
>Mature Secondary Structure
MKKLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEQLPINPSPEFLEFVKENTPLANK
CHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHH
LRNKWLYELARIKDWEDYNKYYQPSNDINLRCFAQIAKFNLGLYKEAIAGSIPIWLSGES
HHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
RPQACNTLFALLLKQDNFDQKFITQRIALALDKRNVLLARYLLKQYKTPHDTEIKNLTSV
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
YENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKLWQEKKTNIMLNERQKQDFLAHL
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECHHHHHHHHHHH
ALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFALKNKNWKQVEKLINNSPNKEMPCW
HHHHHHHCCCCHHHHHHHCCCCHHHHEEEEEEEEEEECCCCHHHHHHHHCCCCCCCCCHH
QYWLARSLEEQGKKAEAIKIYEPLAKNRHYYGFLASKRLKKRPSFDNEKPTTNTEILKPY
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHCCCCCCCCCCCCCCHHCCCH
QPVIEQVKNFYNSKQLLQASRLLNDFISELPKEEASALVYWIATELKWHGKSVYLSNNDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEEHHEECCCEEEECCCCC
LNNQLALRFPLAYKDTIKVYASKYAIPPELVYAIIRQESGFREDVTSSAGAKGLMQVMPQ
CCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHH
TASAVSKTSKIPYSDHKQLFLSQKNINIGIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVY
HHHHHHHHCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHEEE
WLKNHPPKEIDIWIETLPWQETRNYLKNVIAFYIVYQYRLNQQPDMNRFLTPL
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]