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The map label for this gene is bcr [H]

Identifier: 54293277

GI number: 54293277

Start: 370050

End: 371264

Strand: Reverse

Name: bcr [H]

Synonym: lpl0325

Alternate gene names: 54293277

Gene position: 371264-370050 (Counterclockwise)

Preceding gene: 54293281

Following gene: 54293276

Centisome position: 11.1

GC content: 41.07

Gene sequence:

>1215_bases
ATGTATCCAAAAACCAAACTTTCTTTCGTGACTTTATTGTTGTTAATTTCTTTTGCTTCCGTGAATGCTGTCCTATTTAC
ACCGGCATTGCCCAGCATTACTGCATTTTTCGGGATCACAGAACATGCCGCACAGCAGACAATCACCAGTTTTTTGGTTG
GTTATGCCATAGGGCAACTTGTTTATGGCCCTACAGCGAATCGCTATGGAAGGAAAAAAACACTTTATATAGGTATTGGC
TTGCAAATTATCAGCAGTCTTCTATGTGTATTGGCCGGAATGATTTCCATGTACCCATTGCTTGTTTTAGGACGATTTCT
ATTAGCCTTAGGATCCGGTGTCGGACTGAAGATGACTTTTACTTTGGTTAATGAATGCTATGAGCCCCAATTAGCCGCAC
AAAAAACCTCCTATCTGATGCTGGCTTTTGCGATAGCACCCGGTTTGAGTGTTGCACTTGGCGGCATTTTAAACTCCCAT
TACGGCTGGGAAAGTTGCTTTTATGCAGGTATTGTTTATGGTGCTATTCTGCTTGCCATGGTCAGACAGCTTCCCGAAAC
ACAAAAAAATCTTCAACTCGATGCTTTTCAAATCAAGCATCTGCTTCATGGATATGTAAGTCAATTTAAAAACAAGCCAT
TAATCTCTGGTGGAGCATTAATGGGTGGCGCGTCCTGTTTTGTCTACCTTTTTGCAGCGACGGCACCGTTTATTGCCATC
AATTTGCTAGGCATGAGTACAACAGAGTATGGCCTTGCCAATATTTTACCTTCATTAGGTCTGACCCTCGGCTCAATCTG
GTCTGCTCAATTTGCAAAAAAATACTCGCAACGCGCGGGAATCCAATTAGGCATTTTAATCTCAGGGTTTGCCGCACTGG
CAATGATGATGGCCGCGTTAACCCATCATTCAGCGCTTATCAAGCTATTTTTTCCCATGATATTCATCTATTTGGGACTA
TCTTTTATTTTTGCAAATGCTTCGACATTTGCCATGAGCCATACCTCCGATAAAGCCCATGGCTCCGCGGTTATGAACTT
CATCAATATGGGATTGGTTACAGTGGTTATTTTGGGATTAAGCTTACTTCCAATGTCACAATATTTATTACCAGCCATCT
ACATCATTCTATGCATTTTAATGATGTGGCTATTCTTTTCTTTCCTAAAAAGTAATGAAATAATTTCTTCTATCGACAGG
AACAAGGAATCATGA

Upstream 100 bases:

>100_bases
GCAATATAGTTAATAAATGTTAATTAATTATGATATTGTTAATTATTTTGTCAATAATTATACTGTTCTTAACTGTTAAT
CGTTAAAAATTATTCTCATC

Downstream 100 bases:

>100_bases
TGAGTATACAATTGAATCATACTATCGTATGGGTAAAAGATCGGGACAAATCAGCTCACTTCCTTACAGAAATTTTGGGG
TTGCCTGAAGCAATTCCATA

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MYPKTKLSFVTLLLLISFASVNAVLFTPALPSITAFFGITEHAAQQTITSFLVGYAIGQLVYGPTANRYGRKKTLYIGIG
LQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSH
YGWESCFYAGIVYGAILLAMVRQLPETQKNLQLDAFQIKHLLHGYVSQFKNKPLISGGALMGGASCFVYLFAATAPFIAI
NLLGMSTTEYGLANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAALTHHSALIKLFFPMIFIYLGL
SFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVILGLSLLPMSQYLLPAIYIILCILMMWLFFSFLKSNEIISSIDR
NKES

Sequences:

>Translated_404_residues
MYPKTKLSFVTLLLLISFASVNAVLFTPALPSITAFFGITEHAAQQTITSFLVGYAIGQLVYGPTANRYGRKKTLYIGIG
LQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSH
YGWESCFYAGIVYGAILLAMVRQLPETQKNLQLDAFQIKHLLHGYVSQFKNKPLISGGALMGGASCFVYLFAATAPFIAI
NLLGMSTTEYGLANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAALTHHSALIKLFFPMIFIYLGL
SFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVILGLSLLPMSQYLLPAIYIILCILMMWLFFSFLKSNEIISSIDR
NKES
>Mature_404_residues
MYPKTKLSFVTLLLLISFASVNAVLFTPALPSITAFFGITEHAAQQTITSFLVGYAIGQLVYGPTANRYGRKKTLYIGIG
LQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSH
YGWESCFYAGIVYGAILLAMVRQLPETQKNLQLDAFQIKHLLHGYVSQFKNKPLISGGALMGGASCFVYLFAATAPFIAI
NLLGMSTTEYGLANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAALTHHSALIKLFFPMIFIYLGL
SFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVILGLSLLPMSQYLLPAIYIILCILMMWLFFSFLKSNEIISSIDR
NKES

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=277, Percent_Identity=23.826714801444, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082312, Length=263, Percent_Identity=24.3346007604563, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI48994889, Length=347, Percent_Identity=24.4956772334294, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790146, Length=223, Percent_Identity=25.5605381165919, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43790; Mature: 43790

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPKTKLSFVTLLLLISFASVNAVLFTPALPSITAFFGITEHAAQQTITSFLVGYAIGQL
CCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYGPTANRYGRKKTLYIGIGLQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTF
HCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSHYGWESCFYAGIVYGAILLAM
HHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VRQLPETQKNLQLDAFQIKHLLHGYVSQFKNKPLISGGALMGGASCFVYLFAATAPFIAI
HHHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NLLGMSTTEYGLANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAAL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
THHSALIKLFFPMIFIYLGLSFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVILGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
SLLPMSQYLLPAIYIILCILMMWLFFSFLKSNEIISSIDRNKES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
>Mature Secondary Structure
MYPKTKLSFVTLLLLISFASVNAVLFTPALPSITAFFGITEHAAQQTITSFLVGYAIGQL
CCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYGPTANRYGRKKTLYIGIGLQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTF
HCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSHYGWESCFYAGIVYGAILLAM
HHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VRQLPETQKNLQLDAFQIKHLLHGYVSQFKNKPLISGGALMGGASCFVYLFAATAPFIAI
HHHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NLLGMSTTEYGLANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAAL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
THHSALIKLFFPMIFIYLGLSFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVILGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
SLLPMSQYLLPAIYIILCILMMWLFFSFLKSNEIISSIDRNKES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]