Click here to switch to the map view.

The map label for this gene is ndhF [H]

Identifier: 54293228

GI number: 54293228

Start: 307129

End: 308649

Strand: Reverse

Name: ndhF [H]

Synonym: lpl0276

Alternate gene names: 54293228

Gene position: 308649-307129 (Counterclockwise)

Preceding gene: 54293234

Following gene: 54293227

Centisome position: 9.23

GC content: 38.72

Gene sequence:

>1521_bases
GTGGATAAACTTTTAAATGGGTTATCGATTTCACTCATGATATCACCCTTGATAGCGATGCTATTCTATAGCTTGTTAGG
AAAAACAAGAAATATTCAAGCAACTTATTACGGTACCACAGTTATAGGTTTTGGTTTTGGATTGAGTCTCTTGGCATTAA
TTGTTTCTGCAAATACTGTAAACCAAAACAGTTGTTTTTTATTTCATGCCAGCACACTAAGCCTCTTGCTAAGCACATTA
ATATTATTCATAAGCTTTATTATCCACCGTTTTTCCCTACGATACATGCATGGGGACAAGTGCTACCGGCGTTATTTTTT
CAAACTTTCAGCCATAACTTTAACAACTGTTGTGATGGCTTTAGCAGACAATCTTTTATTATTCTGGCTTGCCTGGTCAG
TTAGTAACTTGCTCTTGATAAACCTCATGATTCATAAGTCACAATGGAAAGCAGCCTACAACTCAGGGATGCTGACTTTA
AAAACATTATTACCAGGAAGCTTGTTGCTTATCACTGCATTTTGGATTCTTTTTACATTAAACAATACTCTGTCAATTGA
AACAATAACCAGCGAAGTGAGCAATAAATCATCACATGCTATTGCTGTGGCGCTTGGATTTATTATCTTAACAGCGATGA
CACAATCAGCCATATGGCCTTTTCATCGCTGGCTAATGAGCTCATTAAACTCTCCAACACCGGTATCCGCTCTAATGCAT
GCTGGATTAGTTAATGGAGGGGGCTTTCTGATTGTTAAATTTGCTCCGCTGTTCGTAGCGAAAGGGAATTTATTGACCAT
TTTATTTATTTTCGGCGCACTGACTGCTTTATTAGGCACCCTCTGGAAACTATTGCAGTGGAATATCAAAAGTATGCTTG
CCAGTTCCACCATGGCACAAATGGGTTTTATGATGATGCAATGTGGCTTAGGACTTTTCCCTGCCGCTATCGCCCATCTA
TGCTGGCATGGCTTGTTTAAATCCTATCTTTTTCTCAATTCGGGCTCTGCCGTCCAACAAAAGAAATATCAAAATAAATC
ACGTCCCGAAAAATTGACAAACTTATTGACTGCATTTTTGGGAGGACTTGGTGGAATGTATGGCTTTGCCCTTATCACAG
GGAAACCCATCTTATCTCTCCATGCCTCCACCTTTCTTTTAGGTTTTGTATTCATCACAGGAACACAACTCACCCTGGGC
TTGATTGCATATGAGAAGCATTTCAAAAAAGTAATTTTAGCCTGGGCTTTAGTTTTCTCAATTGGAATTTTTTATGGAGG
AAGTATCCACCTCATCGAGTCACTCTTACCTAATCTAACAGGATTTCCTCAATACCAATTAAGCGGCTTACAAGTCTTAA
CTCTGATTATCTTCTTTTCCCTTTGGCTTGTATTCAGCATGGGGTTATTCAAAAAAATCAGAGAGACCAGACTTTGGGAT
TGGTTTTACATGTACATGTTTAACGCAAGTACACCTCACCCCAAAACCATTACGGCAAATCGCCATACTTATTACTATTA
A

Upstream 100 bases:

>100_bases
AGTTTTTCTAATAGTTTAAATCATTATTTTTAATTTTACTTATATTAACACATCTAATATATTTCCACAATAATAACAGA
TTTTCTAATGGAGAATTAAT

Downstream 100 bases:

>100_bases
GAAAAGGAGACAACATGAGTAGCCACATCACACTAATCAAAACTAACACAAACAATGAAAATAAAATGACCCAATTTAAC
TACAAAGTTGATAATGATGT

Product: hypothetical protein

Products: NA

Alternate protein names: NADH dehydrogenase subunit 5 [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MDKLLNGLSISLMISPLIAMLFYSLLGKTRNIQATYYGTTVIGFGFGLSLLALIVSANTVNQNSCFLFHASTLSLLLSTL
ILFISFIIHRFSLRYMHGDKCYRRYFFKLSAITLTTVVMALADNLLLFWLAWSVSNLLLINLMIHKSQWKAAYNSGMLTL
KTLLPGSLLLITAFWILFTLNNTLSIETITSEVSNKSSHAIAVALGFIILTAMTQSAIWPFHRWLMSSLNSPTPVSALMH
AGLVNGGGFLIVKFAPLFVAKGNLLTILFIFGALTALLGTLWKLLQWNIKSMLASSTMAQMGFMMMQCGLGLFPAAIAHL
CWHGLFKSYLFLNSGSAVQQKKYQNKSRPEKLTNLLTAFLGGLGGMYGFALITGKPILSLHASTFLLGFVFITGTQLTLG
LIAYEKHFKKVILAWALVFSIGIFYGGSIHLIESLLPNLTGFPQYQLSGLQVLTLIIFFSLWLVFSMGLFKKIRETRLWD
WFYMYMFNASTPHPKTITANRHTYYY

Sequences:

>Translated_506_residues
MDKLLNGLSISLMISPLIAMLFYSLLGKTRNIQATYYGTTVIGFGFGLSLLALIVSANTVNQNSCFLFHASTLSLLLSTL
ILFISFIIHRFSLRYMHGDKCYRRYFFKLSAITLTTVVMALADNLLLFWLAWSVSNLLLINLMIHKSQWKAAYNSGMLTL
KTLLPGSLLLITAFWILFTLNNTLSIETITSEVSNKSSHAIAVALGFIILTAMTQSAIWPFHRWLMSSLNSPTPVSALMH
AGLVNGGGFLIVKFAPLFVAKGNLLTILFIFGALTALLGTLWKLLQWNIKSMLASSTMAQMGFMMMQCGLGLFPAAIAHL
CWHGLFKSYLFLNSGSAVQQKKYQNKSRPEKLTNLLTAFLGGLGGMYGFALITGKPILSLHASTFLLGFVFITGTQLTLG
LIAYEKHFKKVILAWALVFSIGIFYGGSIHLIESLLPNLTGFPQYQLSGLQVLTLIIFFSLWLVFSMGLFKKIRETRLWD
WFYMYMFNASTPHPKTITANRHTYYY
>Mature_506_residues
MDKLLNGLSISLMISPLIAMLFYSLLGKTRNIQATYYGTTVIGFGFGLSLLALIVSANTVNQNSCFLFHASTLSLLLSTL
ILFISFIIHRFSLRYMHGDKCYRRYFFKLSAITLTTVVMALADNLLLFWLAWSVSNLLLINLMIHKSQWKAAYNSGMLTL
KTLLPGSLLLITAFWILFTLNNTLSIETITSEVSNKSSHAIAVALGFIILTAMTQSAIWPFHRWLMSSLNSPTPVSALMH
AGLVNGGGFLIVKFAPLFVAKGNLLTILFIFGALTALLGTLWKLLQWNIKSMLASSTMAQMGFMMMQCGLGLFPAAIAHL
CWHGLFKSYLFLNSGSAVQQKKYQNKSRPEKLTNLLTAFLGGLGGMYGFALITGKPILSLHASTFLLGFVFITGTQLTLG
LIAYEKHFKKVILAWALVFSIGIFYGGSIHLIESLLPNLTGFPQYQLSGLQVLTLIIFFSLWLVFSMGLFKKIRETRLWD
WFYMYMFNASTPHPKTITANRHTYYY

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=290, Percent_Identity=29.3103448275862, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1788614, Length=282, Percent_Identity=32.9787234042553, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1788831, Length=350, Percent_Identity=26.2857142857143, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788829, Length=246, Percent_Identity=29.2682926829268, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788827, Length=225, Percent_Identity=26.6666666666667, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56535; Mature: 56535

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLLNGLSISLMISPLIAMLFYSLLGKTRNIQATYYGTTVIGFGFGLSLLALIVSANTV
CCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCC
NQNSCFLFHASTLSLLLSTLILFISFIIHRFSLRYMHGDKCYRRYFFKLSAITLTTVVMA
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADNLLLFWLAWSVSNLLLINLMIHKSQWKAAYNSGMLTLKTLLPGSLLLITAFWILFTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHHHH
NNTLSIETITSEVSNKSSHAIAVALGFIILTAMTQSAIWPFHRWLMSSLNSPTPVSALMH
CCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AGLVNGGGFLIVKFAPLFVAKGNLLTILFIFGALTALLGTLWKLLQWNIKSMLASSTMAQ
CCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGFMMMQCGLGLFPAAIAHLCWHGLFKSYLFLNSGSAVQQKKYQNKSRPEKLTNLLTAFL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCHHHHHHHHHHHH
GGLGGMYGFALITGKPILSLHASTFLLGFVFITGTQLTLGLIAYEKHFKKVILAWALVFS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
IGIFYGGSIHLIESLLPNLTGFPQYQLSGLQVLTLIIFFSLWLVFSMGLFKKIRETRLWD
HHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WFYMYMFNASTPHPKTITANRHTYYY
HHHHHHCCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MDKLLNGLSISLMISPLIAMLFYSLLGKTRNIQATYYGTTVIGFGFGLSLLALIVSANTV
CCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCC
NQNSCFLFHASTLSLLLSTLILFISFIIHRFSLRYMHGDKCYRRYFFKLSAITLTTVVMA
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADNLLLFWLAWSVSNLLLINLMIHKSQWKAAYNSGMLTLKTLLPGSLLLITAFWILFTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHHHH
NNTLSIETITSEVSNKSSHAIAVALGFIILTAMTQSAIWPFHRWLMSSLNSPTPVSALMH
CCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AGLVNGGGFLIVKFAPLFVAKGNLLTILFIFGALTALLGTLWKLLQWNIKSMLASSTMAQ
CCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGFMMMQCGLGLFPAAIAHLCWHGLFKSYLFLNSGSAVQQKKYQNKSRPEKLTNLLTAFL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCHHHHHHHHHHHH
GGLGGMYGFALITGKPILSLHASTFLLGFVFITGTQLTLGLIAYEKHFKKVILAWALVFS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
IGIFYGGSIHLIESLLPNLTGFPQYQLSGLQVLTLIIFFSLWLVFSMGLFKKIRETRLWD
HHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WFYMYMFNASTPHPKTITANRHTYYY
HHHHHHCCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]