| Definition | Legionella pneumophila str. Lens, complete genome. |
|---|---|
| Accession | NC_006369 |
| Length | 3,345,687 |
Click here to switch to the map view.
The map label for this gene is polA [H]
Identifier: 54293062
GI number: 54293062
Start: 115634
End: 118324
Strand: Reverse
Name: polA [H]
Synonym: lpl0099
Alternate gene names: 54293062
Gene position: 118324-115634 (Counterclockwise)
Preceding gene: 54293063
Following gene: 54293058
Centisome position: 3.54
GC content: 41.1
Gene sequence:
>2691_bases ATGAAACCGCCTCTGATTCTAATAGACGGATCTTCCTATTTCTTTAGAGCGTTTCATGCCCTTCCACCATTGACAAACTC CAAAGGCCTGCCTACAGGCGCCATTTATGGAGTTGCCAATATGATTAAAAAAATAATCAAAGATTATCAACCAGAAGAAA TAGCCGTTGTCTTTGATGCCAAAGGGAAAACATTCCGAGATGAATGGTATCCAGAATACAAGGCGCACAGACCACCAATG CCTCAAGAATTAAGCTCTCAGTTTCAACCACTAATCCAGTTACTGCAAGCAATGGGATTGCCCTTGCTTATCATCGATGG AGTCGAAGCAGATGATGTGATTGGCACTTTGGCAAAACAAGCCACAGAGCAAGGCATACCAGTTGTTATATCAACCGGTG ATAAAGACATGGCACAATTGGTCAATGAACATGTTTCCCTCATCAATACCATGAGCAATTACAGTATGGATATTGATGGT GTCAAAGCAAAATTTGGTGTAACACCTGAGCAAATCATTGATTACCTCACTCTCATTGGAGACAGTGTAGATAATATTCC GGGAGTAGAAAAATGCGGCCCAAAAACAGCTGTAAAATGGCTGACAGAGTATCAAACGCTTGATAATTTGCTTGATCACG CCAACGAAATTGGCGGAAAAATTGGTGAATATTTGCGTGCCAGCATCCCTCATTTGCCACTCTCAAAAAAATTGGTCACT ATCAAAACTGATTTGGATTTGCCCTTAAATTTAAATCAATTGATGCCCAAACCGGCTGATAGAGAGCAATTAATTAAATT AACTCGTGAATTAGAATTTAAGTCATGGCTTAAAGAGTTGCTACAGGAAGAAGAGAATCAAAAATCACCCAAAGAAATGG GTGCCAGCAATAATAAATCCTATGAAATAATAACGACTCATCAACAGTTAAATCATTGGCTTAATATGCTAGAGCAAAGT CAACAATTTTGCATCGACACGGAAACAACCAACCTTGATGTCATGCAAGCTGAATTGGTAGGCATCTCTTTAGCAGTTGA GGAAGAAAATGCATCTTATATTCCCCTTGCTCATACTGATGGCAGTACACAGTTAGTAAAAGAAGAAGTTTTAACTGCTT TAAAGCCTATTTTGGAAAATCCAGCTATAGGAAAAATAGGGCAAAACATTAAATATGATTACAGTGTCCTTAAAAATTAT GGCATTACCCTAAAAGGCATTAGGTACGACACCATGCTGGAATCCTATGTGCTTAATAGTGGTGCGGGCAGACATGATAT GGATTCTTTGGCTTTAAAATATCTGGGTTATAAAACAATAAGTTATGAGGATGTTGCGGGAAAGGGAGCCAAACAGCTCC GTTTTGACCAAATTCCAGTAGAGAAAGCCGGGATCTATGCAGCCGAAGACGCTGACATTACTTTGCGATTACATCATAAG CTGTACCCCATGCTGGATAAGCCATTGCGTAACGTGTTGCATGATATCGAAATGCCTTTACTCACTGTTCTCGCAGACAT GGAAATGCACGGCGTTCTCATCGATCCGGTCATTCTGGAAAAGCACGGAAGTCGTTTGAAAGAACAAATGAAATCCCTGG AACAGGAAGCAATTCAACTCGCTGGAAAAGCATTCAATCTCAACTCCCCAAAACAATTACAAGAAATACTATTTGATGAA CAAAAACTGCCTGTTGTAGCGAAAACCCCTACGGGACAGCCTTCAACTGCAGAATCTGTTTTACAGGAATTGGCTTTTGA TTATCGCCTTCCTGCTGTAATTCTGGAATACCGAAGCTTATCAAAATTGGTCAGTACCTATATTGATGCCTTACCCAAAA GAATCAACCCCAAAACACATAGGGTGCATACATCCTATAATCAAGCGGTAGCAGCAACAGGCCGATTATCTTCCAGTGAT CCTAACTTACAAAATATCCCTATACGGAGTGAAGAAGGAAGGTTGATTCGAACTGCCTTCATAGCACCAGAAGGAAGTCT TATCATGGCGGCTGATTACTCGCAAATCGAGTTAAGGATAATGGCGCATTTATCTCAAGATGATAACCTTTTACGAGCTT TTGCTAATGGTTGGGACATCCATGCCGCAACGGCGAGTGAAATCTTTCAAACGAATTTGGATGCTGTGTCCAAAGAACAA CGAAGGCGTGCCAAGGCAGTGAACTTTGGTTTGATATATGGTATGTCTGCCTTTGGACTGGCAAAACAAATTGGTGTGGA GCGTCAGGATGCTCAACATTATATTGATACTTATTTCCGCCGTTATCCTAAGGTACTTGAATACATGGAGCGTACTCGCA AACAAGCGCATCAACTTGGTTATGTTGAAACCTTGTTTGGCCGTCGTCTCTACTTGCCTGAAATTAATTCACGTAACCTA ATGAGACAAAAAGCAGCTGAGCGCACTGCTATCAACGGACCAATGCAAGGAACCGCAGCTGATATTATAAAAAAAACGAT GCTGGCTGTTTCCAATTGGGAGCATTCGCAAAAGAACCCCTCTGCAAAGATGATCATGCAGGTACATGATGAATTGGTTT TTGAAGTCCGAAAGGAAGCGGTTGAAGAATGTCGCGCCATTATCCACAAGCTTATGGAACAAACTGTAAAATTATCAGTG CCTCTTGTTGTGTCTATTGGCACAGGTTCTAACTGGGATGATGCGCATTGA
Upstream 100 bases:
>100_bases ATCGTATCCCATTAATACGAAAAAACATTAAATCACTTACCGAGCAAGTGGCTGTAATTAACAAAAAACTTGATATTAAA GCAAAAGAGGTAGAGTAATT
Downstream 100 bases:
>100_bases AGCGCATAAAAGCGTTTAATTTATTGGATTTAATAATAATTAATAGAAGTTTGACTCGCAAAGGAAGGATTTTGACCACC TATAAACTCACCAAAATAAT
Product: DNA polymerase I
Products: NA
Alternate protein names: POL I [H]
Number of amino acids: Translated: 896; Mature: 896
Protein sequence:
>896_residues MKPPLILIDGSSYFFRAFHALPPLTNSKGLPTGAIYGVANMIKKIIKDYQPEEIAVVFDAKGKTFRDEWYPEYKAHRPPM PQELSSQFQPLIQLLQAMGLPLLIIDGVEADDVIGTLAKQATEQGIPVVISTGDKDMAQLVNEHVSLINTMSNYSMDIDG VKAKFGVTPEQIIDYLTLIGDSVDNIPGVEKCGPKTAVKWLTEYQTLDNLLDHANEIGGKIGEYLRASIPHLPLSKKLVT IKTDLDLPLNLNQLMPKPADREQLIKLTRELEFKSWLKELLQEEENQKSPKEMGASNNKSYEIITTHQQLNHWLNMLEQS QQFCIDTETTNLDVMQAELVGISLAVEEENASYIPLAHTDGSTQLVKEEVLTALKPILENPAIGKIGQNIKYDYSVLKNY GITLKGIRYDTMLESYVLNSGAGRHDMDSLALKYLGYKTISYEDVAGKGAKQLRFDQIPVEKAGIYAAEDADITLRLHHK LYPMLDKPLRNVLHDIEMPLLTVLADMEMHGVLIDPVILEKHGSRLKEQMKSLEQEAIQLAGKAFNLNSPKQLQEILFDE QKLPVVAKTPTGQPSTAESVLQELAFDYRLPAVILEYRSLSKLVSTYIDALPKRINPKTHRVHTSYNQAVAATGRLSSSD PNLQNIPIRSEEGRLIRTAFIAPEGSLIMAADYSQIELRIMAHLSQDDNLLRAFANGWDIHAATASEIFQTNLDAVSKEQ RRRAKAVNFGLIYGMSAFGLAKQIGVERQDAQHYIDTYFRRYPKVLEYMERTRKQAHQLGYVETLFGRRLYLPEINSRNL MRQKAAERTAINGPMQGTAADIIKKTMLAVSNWEHSQKNPSAKMIMQVHDELVFEVRKEAVEECRAIIHKLMEQTVKLSV PLVVSIGTGSNWDDAH
Sequences:
>Translated_896_residues MKPPLILIDGSSYFFRAFHALPPLTNSKGLPTGAIYGVANMIKKIIKDYQPEEIAVVFDAKGKTFRDEWYPEYKAHRPPM PQELSSQFQPLIQLLQAMGLPLLIIDGVEADDVIGTLAKQATEQGIPVVISTGDKDMAQLVNEHVSLINTMSNYSMDIDG VKAKFGVTPEQIIDYLTLIGDSVDNIPGVEKCGPKTAVKWLTEYQTLDNLLDHANEIGGKIGEYLRASIPHLPLSKKLVT IKTDLDLPLNLNQLMPKPADREQLIKLTRELEFKSWLKELLQEEENQKSPKEMGASNNKSYEIITTHQQLNHWLNMLEQS QQFCIDTETTNLDVMQAELVGISLAVEEENASYIPLAHTDGSTQLVKEEVLTALKPILENPAIGKIGQNIKYDYSVLKNY GITLKGIRYDTMLESYVLNSGAGRHDMDSLALKYLGYKTISYEDVAGKGAKQLRFDQIPVEKAGIYAAEDADITLRLHHK LYPMLDKPLRNVLHDIEMPLLTVLADMEMHGVLIDPVILEKHGSRLKEQMKSLEQEAIQLAGKAFNLNSPKQLQEILFDE QKLPVVAKTPTGQPSTAESVLQELAFDYRLPAVILEYRSLSKLVSTYIDALPKRINPKTHRVHTSYNQAVAATGRLSSSD PNLQNIPIRSEEGRLIRTAFIAPEGSLIMAADYSQIELRIMAHLSQDDNLLRAFANGWDIHAATASEIFQTNLDAVSKEQ RRRAKAVNFGLIYGMSAFGLAKQIGVERQDAQHYIDTYFRRYPKVLEYMERTRKQAHQLGYVETLFGRRLYLPEINSRNL MRQKAAERTAINGPMQGTAADIIKKTMLAVSNWEHSQKNPSAKMIMQVHDELVFEVRKEAVEECRAIIHKLMEQTVKLSV PLVVSIGTGSNWDDAH >Mature_896_residues MKPPLILIDGSSYFFRAFHALPPLTNSKGLPTGAIYGVANMIKKIIKDYQPEEIAVVFDAKGKTFRDEWYPEYKAHRPPM PQELSSQFQPLIQLLQAMGLPLLIIDGVEADDVIGTLAKQATEQGIPVVISTGDKDMAQLVNEHVSLINTMSNYSMDIDG VKAKFGVTPEQIIDYLTLIGDSVDNIPGVEKCGPKTAVKWLTEYQTLDNLLDHANEIGGKIGEYLRASIPHLPLSKKLVT IKTDLDLPLNLNQLMPKPADREQLIKLTRELEFKSWLKELLQEEENQKSPKEMGASNNKSYEIITTHQQLNHWLNMLEQS QQFCIDTETTNLDVMQAELVGISLAVEEENASYIPLAHTDGSTQLVKEEVLTALKPILENPAIGKIGQNIKYDYSVLKNY GITLKGIRYDTMLESYVLNSGAGRHDMDSLALKYLGYKTISYEDVAGKGAKQLRFDQIPVEKAGIYAAEDADITLRLHHK LYPMLDKPLRNVLHDIEMPLLTVLADMEMHGVLIDPVILEKHGSRLKEQMKSLEQEAIQLAGKAFNLNSPKQLQEILFDE QKLPVVAKTPTGQPSTAESVLQELAFDYRLPAVILEYRSLSKLVSTYIDALPKRINPKTHRVHTSYNQAVAATGRLSSSD PNLQNIPIRSEEGRLIRTAFIAPEGSLIMAADYSQIELRIMAHLSQDDNLLRAFANGWDIHAATASEIFQTNLDAVSKEQ RRRAKAVNFGLIYGMSAFGLAKQIGVERQDAQHYIDTYFRRYPKVLEYMERTRKQAHQLGYVETLFGRRLYLPEINSRNL MRQKAAERTAINGPMQGTAADIIKKTMLAVSNWEHSQKNPSAKMIMQVHDELVFEVRKEAVEECRAIIHKLMEQTVKLSV PLVVSIGTGSNWDDAH
Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]
COG id: COG0749
COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 5'-3' exonuclease domain [H]
Homologues:
Organism=Homo sapiens, GI112420958, Length=565, Percent_Identity=30.6194690265487, Blast_Score=245, Evalue=2e-64, Organism=Homo sapiens, GI139394648, Length=613, Percent_Identity=27.8955954323002, Blast_Score=209, Evalue=1e-53, Organism=Escherichia coli, GI1790294, Length=923, Percent_Identity=55.6879739978332, Blast_Score=979, Evalue=0.0, Organism=Escherichia coli, GI226510970, Length=252, Percent_Identity=29.7619047619048, Blast_Score=122, Evalue=1e-28, Organism=Caenorhabditis elegans, GI86563272, Length=410, Percent_Identity=28.780487804878, Blast_Score=165, Evalue=1e-40, Organism=Drosophila melanogaster, GI17933644, Length=422, Percent_Identity=32.2274881516588, Blast_Score=196, Evalue=7e-50,
Paralogues:
None
Copy number: 400 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002562 - InterPro: IPR020046 - InterPro: IPR020045 - InterPro: IPR002421 - InterPro: IPR020047 - InterPro: IPR019760 - InterPro: IPR001098 - InterPro: IPR018320 - InterPro: IPR002298 - InterPro: IPR008918 - InterPro: IPR012337 [H]
Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 100730; Mature: 100730
Theoretical pI: Translated: 6.32; Mature: 6.32
Prosite motif: PS00447 DNA_POLYMERASE_A
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKPPLILIDGSSYFFRAFHALPPLTNSKGLPTGAIYGVANMIKKIIKDYQPEEIAVVFDA CCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC KGKTFRDEWYPEYKAHRPPMPQELSSQFQPLIQLLQAMGLPLLIIDGVEADDVIGTLAKQ CCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHH ATEQGIPVVISTGDKDMAQLVNEHVSLINTMSNYSMDIDGVKAKFGVTPEQIIDYLTLIG HHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHC DSVDNIPGVEKCGPKTAVKWLTEYQTLDNLLDHANEIGGKIGEYLRASIPHLPLSKKLVT CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE IKTDLDLPLNLNQLMPKPADREQLIKLTRELEFKSWLKELLQEEENQKSPKEMGASNNKS EEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC YEIITTHQQLNHWLNMLEQSQQFCIDTETTNLDVMQAELVGISLAVEEENASYIPLAHTD EEEEECHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHEEEEEEEECCCCCEEEEEECC GSTQLVKEEVLTALKPILENPAIGKIGQNIKYDYSVLKNYGITLKGIRYDTMLESYVLNS CCHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHCC GAGRHDMDSLALKYLGYKTISYEDVAGKGAKQLRFDQIPVEKAGIYAAEDADITLRLHHK CCCCCHHHHHHHHHHCCCCCCCHHHCCCCHHHCCCCCCCCHHCCEEEECCCCEEEEEHHH LYPMLDKPLRNVLHDIEMPLLTVLADMEMHGVLIDPVILEKHGSRLKEQMKSLEQEAIQL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH AGKAFNLNSPKQLQEILFDEQKLPVVAKTPTGQPSTAESVLQELAFDYRLPAVILEYRSL HCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH SKLVSTYIDALPKRINPKTHRVHTSYNQAVAATGRLSSSDPNLQNIPIRSEEGRLIRTAF HHHHHHHHHHHHHHCCCCHHEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEE IAPEGSLIMAADYSQIELRIMAHLSQDDNLLRAFANGWDIHAATASEIFQTNLDAVSKEQ ECCCCCEEEEECCCHHEEHHHHHCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH RRRAKAVNFGLIYGMSAFGLAKQIGVERQDAQHYIDTYFRRYPKVLEYMERTRKQAHQLG HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC YVETLFGRRLYLPEINSRNLMRQKAAERTAINGPMQGTAADIIKKTMLAVSNWEHSQKNP HHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC SAKMIMQVHDELVFEVRKEAVEECRAIIHKLMEQTVKLSVPLVVSIGTGSNWDDAH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC >Mature Secondary Structure MKPPLILIDGSSYFFRAFHALPPLTNSKGLPTGAIYGVANMIKKIIKDYQPEEIAVVFDA CCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC KGKTFRDEWYPEYKAHRPPMPQELSSQFQPLIQLLQAMGLPLLIIDGVEADDVIGTLAKQ CCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHH ATEQGIPVVISTGDKDMAQLVNEHVSLINTMSNYSMDIDGVKAKFGVTPEQIIDYLTLIG HHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHC DSVDNIPGVEKCGPKTAVKWLTEYQTLDNLLDHANEIGGKIGEYLRASIPHLPLSKKLVT CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE IKTDLDLPLNLNQLMPKPADREQLIKLTRELEFKSWLKELLQEEENQKSPKEMGASNNKS EEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC YEIITTHQQLNHWLNMLEQSQQFCIDTETTNLDVMQAELVGISLAVEEENASYIPLAHTD EEEEECHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHEEEEEEEECCCCCEEEEEECC GSTQLVKEEVLTALKPILENPAIGKIGQNIKYDYSVLKNYGITLKGIRYDTMLESYVLNS CCHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHCC GAGRHDMDSLALKYLGYKTISYEDVAGKGAKQLRFDQIPVEKAGIYAAEDADITLRLHHK CCCCCHHHHHHHHHHCCCCCCCHHHCCCCHHHCCCCCCCCHHCCEEEECCCCEEEEEHHH LYPMLDKPLRNVLHDIEMPLLTVLADMEMHGVLIDPVILEKHGSRLKEQMKSLEQEAIQL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH AGKAFNLNSPKQLQEILFDEQKLPVVAKTPTGQPSTAESVLQELAFDYRLPAVILEYRSL HCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH SKLVSTYIDALPKRINPKTHRVHTSYNQAVAATGRLSSSDPNLQNIPIRSEEGRLIRTAF HHHHHHHHHHHHHHCCCCHHEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEE IAPEGSLIMAADYSQIELRIMAHLSQDDNLLRAFANGWDIHAATASEIFQTNLDAVSKEQ ECCCCCEEEEECCCHHEEHHHHHCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH RRRAKAVNFGLIYGMSAFGLAKQIGVERQDAQHYIDTYFRRYPKVLEYMERTRKQAHQLG HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC YVETLFGRRLYLPEINSRNLMRQKAAERTAINGPMQGTAADIIKKTMLAVSNWEHSQKNP HHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC SAKMIMQVHDELVFEVRKEAVEECRAIIHKLMEQTVKLSVPLVVSIGTGSNWDDAH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043 [H]