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The map label for this gene is dinB2 [H]

Identifier: 54027589

GI number: 54027589

Start: 5954376

End: 5955443

Strand: Reverse

Name: dinB2 [H]

Synonym: nfa56150

Alternate gene names: 54027589

Gene position: 5955443-5954376 (Counterclockwise)

Preceding gene: 54027592

Following gene: 54027587

Centisome position: 98.91

GC content: 71.44

Gene sequence:

>1068_bases
GTGGCCAGATGGCTGTTGCACGTCGATCTCGACCAGTTCCAGGCGGCGGTGGAGTTCCGGCGCCACCCGGAACTGCGCGG
CCGTCCGGTGATCGTCGGTGGCAACGGTGACCCGCAGGAACCGCGCAAGGTCGTCACCTGCGCGTCCTATCCGGCACGGG
CGTACGGGGTGCGGGCGGGCATGCCGTTGCGCGCGGCGGCTCGCAAGTGCCCCGACGGCGTGTTCCTCCCGTTGGACATG
GCCGCCTACGAGGATGCCTCCGACGAGGTGATGGCGGTGCTGCGCACGTTTCCAGGCACCGTGGAGGTGTGGGGTTGGGA
CGAGGCGTTCCTGGCCGCCGACACCGACGACCCGGAACTGCTGGCCCGCGAGATCCGCGGCGCGATCGCGGAGCTGGATC
TGGGCTGCGCGATCGGTATCGGCGACAACAAGCTCACCGCCAAGCTGGCCACCGGCTTCGCCAAGTCGGCGGGTAAATCC
TCCGCCGCACCCGCCGACGACCCCGGCGCGGCCACCGGCACCTTCCGGCTCACCGCCGCGAACTGGGCCGAGGTGATGGG
CCACCGCCCCACCGCCGCGCTGTGGGGCATCGGCAACCGCATCGCGGCCCGGCTGGCCGGCCTGGGCATCGAGACCGTCG
CCGACCTGATGGCCGCCGACCGGCACCGGCTGGCCGCCGAGTTCGGGCCGAGCACCGGCCCGTACCTGTGGGTGCTCGGG
CACGGCAAAGGCGACACCGAGGTCAGCAGCGAACCACGAGTGCCGGTGGGCCGCAGCCGATCCGAGACCTTCCCCCGCGA
CCTCACCGACCCCGCGGAGATCAGGGCGCAGGTCGCGCGGATCGCCACCGCGGTCGCCGAGGAGATGGCCGAGGCCGGAC
GGATCACCGTCCGGGTCTCGGTCACGGTGCGGACCAAGACCTTCTTCACCCGCAGCAAGCAGACGAAGCTACCCGAACCG
ACCGCCGACGTCGCCACCATCACCGAGGCGGCGTTGCGGGTGATCGACCGATTCGAGCTCGACCGTCCGGTCCGATTGCT
CGGCGTACGACTGGAATTCGCGCCCTGA

Upstream 100 bases:

>100_bases
ATATCCAGAATCTAGTTCTGTTTTCCGGGTGTGGCAAGAGAAGAACCGCTGCTCGTGGTGGCGGATGGGCTGAGGCGGCG
CGGTGGGGTAAGACGGAGAG

Downstream 100 bases:

>100_bases
ACCCGGTGCTGGTCAGCGGCGCTGCAACCAGACCGAGGCCAGCGGATACGGCAGGATCACCGGCGTATCGGCGACGTTCA
CCGTCCCGGTCCACATGCCC

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV 2 [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MARWLLHVDLDQFQAAVEFRRHPELRGRPVIVGGNGDPQEPRKVVTCASYPARAYGVRAGMPLRAAARKCPDGVFLPLDM
AAYEDASDEVMAVLRTFPGTVEVWGWDEAFLAADTDDPELLAREIRGAIAELDLGCAIGIGDNKLTAKLATGFAKSAGKS
SAAPADDPGAATGTFRLTAANWAEVMGHRPTAALWGIGNRIAARLAGLGIETVADLMAADRHRLAAEFGPSTGPYLWVLG
HGKGDTEVSSEPRVPVGRSRSETFPRDLTDPAEIRAQVARIATAVAEEMAEAGRITVRVSVTVRTKTFFTRSKQTKLPEP
TADVATITEAALRVIDRFELDRPVRLLGVRLEFAP

Sequences:

>Translated_355_residues
MARWLLHVDLDQFQAAVEFRRHPELRGRPVIVGGNGDPQEPRKVVTCASYPARAYGVRAGMPLRAAARKCPDGVFLPLDM
AAYEDASDEVMAVLRTFPGTVEVWGWDEAFLAADTDDPELLAREIRGAIAELDLGCAIGIGDNKLTAKLATGFAKSAGKS
SAAPADDPGAATGTFRLTAANWAEVMGHRPTAALWGIGNRIAARLAGLGIETVADLMAADRHRLAAEFGPSTGPYLWVLG
HGKGDTEVSSEPRVPVGRSRSETFPRDLTDPAEIRAQVARIATAVAEEMAEAGRITVRVSVTVRTKTFFTRSKQTKLPEP
TADVATITEAALRVIDRFELDRPVRLLGVRLEFAP
>Mature_354_residues
ARWLLHVDLDQFQAAVEFRRHPELRGRPVIVGGNGDPQEPRKVVTCASYPARAYGVRAGMPLRAAARKCPDGVFLPLDMA
AYEDASDEVMAVLRTFPGTVEVWGWDEAFLAADTDDPELLAREIRGAIAELDLGCAIGIGDNKLTAKLATGFAKSAGKSS
AAPADDPGAATGTFRLTAANWAEVMGHRPTAALWGIGNRIAARLAGLGIETVADLMAADRHRLAAEFGPSTGPYLWVLGH
GKGDTEVSSEPRVPVGRSRSETFPRDLTDPAEIRAQVARIATAVAEEMAEAGRITVRVSVTVRTKTFFTRSKQTKLPEPT
ADVATITEAALRVIDRFELDRPVRLLGVRLEFAP

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI84043967, Length=357, Percent_Identity=25.2100840336134, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI7706681, Length=358, Percent_Identity=25.1396648044693, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI7705344, Length=111, Percent_Identity=34.2342342342342, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786425, Length=360, Percent_Identity=30, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1787432, Length=345, Percent_Identity=26.9565217391304, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17537959, Length=238, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355641, Length=349, Percent_Identity=25.7879656160458, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24644984, Length=349, Percent_Identity=25.7879656160458, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 38190; Mature: 38059

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARWLLHVDLDQFQAAVEFRRHPELRGRPVIVGGNGDPQEPRKVVTCASYPARAYGVRAG
CCEEEEEECHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHEEEECCCCCHHHCCCCC
MPLRAAARKCPDGVFLPLDMAAYEDASDEVMAVLRTFPGTVEVWGWDEAFLAADTDDPEL
CCHHHHHHHCCCCEEEECCHHHCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHH
LAREIRGAIAELDLGCAIGIGDNKLTAKLATGFAKSAGKSSAAPADDPGAATGTFRLTAA
HHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEC
NWAEVMGHRPTAALWGIGNRIAARLAGLGIETVADLMAADRHRLAAEFGPSTGPYLWVLG
HHHHHHCCCCCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
HGKGDTEVSSEPRVPVGRSRSETFPRDLTDPAEIRAQVARIATAVAEEMAEAGRITVRVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
VTVRTKTFFTRSKQTKLPEPTADVATITEAALRVIDRFELDRPVRLLGVRLEFAP
EEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC
>Mature Secondary Structure 
ARWLLHVDLDQFQAAVEFRRHPELRGRPVIVGGNGDPQEPRKVVTCASYPARAYGVRAG
CEEEEEECHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHEEEECCCCCHHHCCCCC
MPLRAAARKCPDGVFLPLDMAAYEDASDEVMAVLRTFPGTVEVWGWDEAFLAADTDDPEL
CCHHHHHHHCCCCEEEECCHHHCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHH
LAREIRGAIAELDLGCAIGIGDNKLTAKLATGFAKSAGKSSAAPADDPGAATGTFRLTAA
HHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEC
NWAEVMGHRPTAALWGIGNRIAARLAGLGIETVADLMAADRHRLAAEFGPSTGPYLWVLG
HHHHHHCCCCCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
HGKGDTEVSSEPRVPVGRSRSETFPRDLTDPAEIRAQVARIATAVAEEMAEAGRITVRVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
VTVRTKTFFTRSKQTKLPEPTADVATITEAALRVIDRFELDRPVRLLGVRLEFAP
EEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]