Click here to switch to the map view.

The map label for this gene is clpB [H]

Identifier: 54027376

GI number: 54027376

Start: 5704757

End: 5707318

Strand: Reverse

Name: clpB [H]

Synonym: nfa54020

Alternate gene names: 54027376

Gene position: 5707318-5704757 (Counterclockwise)

Preceding gene: 54027379

Following gene: 54027375

Centisome position: 94.79

GC content: 69.95

Gene sequence:

>2562_bases
GTGGACTCGTTCAACCCCACCACCAAGACCCAGGCGGCCCTGACCGCGGCCTTGCAGGCCGCCTCGGCCGCGGGCAACCC
GGAGATCCGGCCCGCACACCTGTTGGTGGCGTTGCTGGATCAGACCGACGGCATCGCCGCACCGCTGCTCAAGGCCGTCG
GGGTGGACCCGGCGACGGTGCGGCGGGAGGCGCAGGACCTCGTCGACCGGCTGCCCCGCGCCACCGGCGCGACCACCCAG
CCGCAGCTGGGCCGGGAGGCGCTGGCCGCCATCACCGCCGCGCAGCGGCTGGCGACCGAACTCGGTGACGAGTACGTCTC
CACCGAACACGTCCTGGTGGGGCTGGCCGACGGCGATTCCGACGTCACGATGCTGCTGAAGAAGTACGGCGCCACCGCCG
ACGCGCTGCGCGAGGCATTCACGACCGTGCGCGGCAGCGCGCGCGTCACCACGCCCGACCCCGAGGCCACCTACCAGGCG
CTGGAGAAGTACTCCACCGATCTCACCGAGGCCGCGCGCACCGGCAAGCTCGATCCGGTGATCGGCCGGGACACCGAGAT
CCGCCGCGTCGTGCAGGTGCTGAGCCGGCGCACCAAGAACAACCCGGTGCTGATCGGTGAGCCCGGCGTCGGCAAGACCG
CGATCGTGGAGGGGCTCGCCCAGCGCATCGTGGCCGGTGACGTGCCGGAGTCGCTGCGCGGCAAGAAGGTGATCTCGCTG
GATCTGGGCGCGATGGTGGCGGGTGCGAAGTACCGCGGCGAGTTCGAGGAGCGGCTCAAGGCCGTGCTCGAGGACATCAA
GAACAGCGCGGGCCAGATCATCACGTTCATCGACGAGCTGCACACCATCGTCGGCGCGGGCGCGACCGGCGAATCGGCGA
TGGACGCGGGCAACATGATCAAGCCGATGCTGGCGCGCGGTGAGCTGCGGCTGGTCGGCGCGACCACGCTGGAGGAGTAC
CGCCAGCACATCGAGAAGGACGCCGCGCTCGAGCGGCGCTTCCAGCAGGTGCTGGTCGGTGAGCCCTCGGTGGAGGACAC
CGTCGGCATCCTGCGCGGCATCAAGGAGCGCTACGAGGTGCACCACGGCGTGCGGATCACCGACTCGGCGCTGGTGGCCG
CGGCGGCGCTGTCCGACCGCTACATCACCTCGCGTTTCCTGCCGGACAAGGCGATCGACCTGGTCGACGAGTCCGCTTCC
CGGCTGCGCATGGAGATCGACTCCCGCCCGGTCGAGATCGACGAGGTCGAGCGCGCGGTGCGGCGGCTGGAGATCGAAGA
GGTCGCGCTGAGCAAGGAGACCGACGAGGCCTCCAAGTTGCGGCTGGAGAAGCTGCGCCAGGAACTGGCCGACGACCGGG
AGAAGCTGAACCAGCTGATGACCCGGTGGCAGAACGAGAAGAGCGCTATCGACCAGGTCCGCACGCTCAAGGAACAGCTC
GAGGCGCTGCGCGGGGAGTCCGAGCGGGCCGAGCGCGACGGCGATCTGGGCAAGGCCGCCGAGCTGCGCTACGGCCGCAT
CCCCAGTCTGGAGAAGCAGCTGGCCGAGGCCGAGAAGGCCTCCGGTGCGGCCGCCGACGGCGAGGTGATGCTCAAGGAGG
AGGTCGGCCCCGACGACATCGCCGAGGTGGTGTCCTCCTGGACCGGCATTCCGGTCGGCAGGCTGCTCGAGGGTGAGACC
CAGAAGCTGCTGCGGATGGAGGACGAGCTGGGCCGTCGCGTGGTCGGACAGAAGGAGGCCGTGCAGGCGGTGTCGGACGC
GGTGCGCCGGGCGCGGGCCGGAGTCGCCGACCCGAACCGGCCGACCGGCTCGTTCATGTTCGTCGGACCGACCGGCGTCG
GCAAGACCGAGCTGGCCAAGGCGCTGGCCGACTTCCTGTTCGACGACGAGCGCGCGATGGTGCGTATCGACATGAGCGAG
TACAGCGAGAAGCACGCGGTGGCTCGCCTGGTCGGCGCGCCTCCCGGCTACGTCGGCTACGACCAGGGCGGCCAGCTCAC
CGAGGCGGTGCGGCGCAGGCCGTACACGGTGGTGCTGTTCGACGAGATCGAGAAGGCGCACCCGGACGTCTTCGACATCC
TGCTGCAGGTGCTCGACGAGGGCAGGTTGACCGACGGCCAGGGCCGCACGGTCGACTTCCGCAACACCATCCTCATCCTC
ACCTCCAACCTGGGCGCGGGTGGTGACCAGGAGTTCGTCATGAACGCGGTGCGGGCCGCCTTCAAGCCGGAGTTCCTCAA
CCGGCTCGACGACGTGGTCATGTTCCACGCGCTCGACGAGGAACAGCTCGAGCACATCGTCGACATCCAGCTCGAGCAGC
TGCAGAAGCGCCTGGCGCAGCGGCGGCTCACCCTCGATGTCACCGGCTCGGCGCGGTTCTGGCTGGCGGTGCGCGGCTAC
GACCCCGTCTACGGCGCGCGGCCACTGCGCAGGCTGATCCAGCAGGCCATCGGCGACACCCTCGCCAAGGAACTGTTGGC
GGGCACCGTCACCGACGGCGACACCGTCAAGGTGGGTGTCAGCCCCGACGGCGACAACCTGGTCGTCGAACCGGCGTCCT
GA

Upstream 100 bases:

>100_bases
TAAACTTGAGCGGAACAGACTCAACCTCTCCCACGTTGGATCCGGTAGGAGCAGTAGAGCCGCGGCCCTACTGCACAGGC
GACTAGTAGGAAGGTGTCCC

Downstream 100 bases:

>100_bases
TCACCGAACGTCCCCATCGGCGCACGCCGGTGGGGACGTTCCGCGTGCCCGGCACGGTGGACACAGCCGGGCCGAAGGTG
CGGCGAATCGCATCCCGCGC

Product: putative Clp protease ATP-binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 853; Mature: 853

Protein sequence:

>853_residues
MDSFNPTTKTQAALTAALQAASAAGNPEIRPAHLLVALLDQTDGIAAPLLKAVGVDPATVRREAQDLVDRLPRATGATTQ
PQLGREALAAITAAQRLATELGDEYVSTEHVLVGLADGDSDVTMLLKKYGATADALREAFTTVRGSARVTTPDPEATYQA
LEKYSTDLTEAARTGKLDPVIGRDTEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPESLRGKKVISL
DLGAMVAGAKYRGEFEERLKAVLEDIKNSAGQIITFIDELHTIVGAGATGESAMDAGNMIKPMLARGELRLVGATTLEEY
RQHIEKDAALERRFQQVLVGEPSVEDTVGILRGIKERYEVHHGVRITDSALVAAAALSDRYITSRFLPDKAIDLVDESAS
RLRMEIDSRPVEIDEVERAVRRLEIEEVALSKETDEASKLRLEKLRQELADDREKLNQLMTRWQNEKSAIDQVRTLKEQL
EALRGESERAERDGDLGKAAELRYGRIPSLEKQLAEAEKASGAAADGEVMLKEEVGPDDIAEVVSSWTGIPVGRLLEGET
QKLLRMEDELGRRVVGQKEAVQAVSDAVRRARAGVADPNRPTGSFMFVGPTGVGKTELAKALADFLFDDERAMVRIDMSE
YSEKHAVARLVGAPPGYVGYDQGGQLTEAVRRRPYTVVLFDEIEKAHPDVFDILLQVLDEGRLTDGQGRTVDFRNTILIL
TSNLGAGGDQEFVMNAVRAAFKPEFLNRLDDVVMFHALDEEQLEHIVDIQLEQLQKRLAQRRLTLDVTGSARFWLAVRGY
DPVYGARPLRRLIQQAIGDTLAKELLAGTVTDGDTVKVGVSPDGDNLVVEPAS

Sequences:

>Translated_853_residues
MDSFNPTTKTQAALTAALQAASAAGNPEIRPAHLLVALLDQTDGIAAPLLKAVGVDPATVRREAQDLVDRLPRATGATTQ
PQLGREALAAITAAQRLATELGDEYVSTEHVLVGLADGDSDVTMLLKKYGATADALREAFTTVRGSARVTTPDPEATYQA
LEKYSTDLTEAARTGKLDPVIGRDTEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPESLRGKKVISL
DLGAMVAGAKYRGEFEERLKAVLEDIKNSAGQIITFIDELHTIVGAGATGESAMDAGNMIKPMLARGELRLVGATTLEEY
RQHIEKDAALERRFQQVLVGEPSVEDTVGILRGIKERYEVHHGVRITDSALVAAAALSDRYITSRFLPDKAIDLVDESAS
RLRMEIDSRPVEIDEVERAVRRLEIEEVALSKETDEASKLRLEKLRQELADDREKLNQLMTRWQNEKSAIDQVRTLKEQL
EALRGESERAERDGDLGKAAELRYGRIPSLEKQLAEAEKASGAAADGEVMLKEEVGPDDIAEVVSSWTGIPVGRLLEGET
QKLLRMEDELGRRVVGQKEAVQAVSDAVRRARAGVADPNRPTGSFMFVGPTGVGKTELAKALADFLFDDERAMVRIDMSE
YSEKHAVARLVGAPPGYVGYDQGGQLTEAVRRRPYTVVLFDEIEKAHPDVFDILLQVLDEGRLTDGQGRTVDFRNTILIL
TSNLGAGGDQEFVMNAVRAAFKPEFLNRLDDVVMFHALDEEQLEHIVDIQLEQLQKRLAQRRLTLDVTGSARFWLAVRGY
DPVYGARPLRRLIQQAIGDTLAKELLAGTVTDGDTVKVGVSPDGDNLVVEPAS
>Mature_853_residues
MDSFNPTTKTQAALTAALQAASAAGNPEIRPAHLLVALLDQTDGIAAPLLKAVGVDPATVRREAQDLVDRLPRATGATTQ
PQLGREALAAITAAQRLATELGDEYVSTEHVLVGLADGDSDVTMLLKKYGATADALREAFTTVRGSARVTTPDPEATYQA
LEKYSTDLTEAARTGKLDPVIGRDTEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPESLRGKKVISL
DLGAMVAGAKYRGEFEERLKAVLEDIKNSAGQIITFIDELHTIVGAGATGESAMDAGNMIKPMLARGELRLVGATTLEEY
RQHIEKDAALERRFQQVLVGEPSVEDTVGILRGIKERYEVHHGVRITDSALVAAAALSDRYITSRFLPDKAIDLVDESAS
RLRMEIDSRPVEIDEVERAVRRLEIEEVALSKETDEASKLRLEKLRQELADDREKLNQLMTRWQNEKSAIDQVRTLKEQL
EALRGESERAERDGDLGKAAELRYGRIPSLEKQLAEAEKASGAAADGEVMLKEEVGPDDIAEVVSSWTGIPVGRLLEGET
QKLLRMEDELGRRVVGQKEAVQAVSDAVRRARAGVADPNRPTGSFMFVGPTGVGKTELAKALADFLFDDERAMVRIDMSE
YSEKHAVARLVGAPPGYVGYDQGGQLTEAVRRRPYTVVLFDEIEKAHPDVFDILLQVLDEGRLTDGQGRTVDFRNTILIL
TSNLGAGGDQEFVMNAVRAAFKPEFLNRLDDVVMFHALDEEQLEHIVDIQLEQLQKRLAQRRLTLDVTGSARFWLAVRGY
DPVYGARPLRRLIQQAIGDTLAKELLAGTVTDGDTVKVGVSPDGDNLVVEPAS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=371, Percent_Identity=32.8840970350404, Blast_Score=200, Evalue=4e-51,
Organism=Escherichia coli, GI1788943, Length=849, Percent_Identity=55.5948174322733, Blast_Score=940, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=408, Percent_Identity=40.1960784313725, Blast_Score=288, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6320464, Length=716, Percent_Identity=54.7486033519553, Blast_Score=781, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=861, Percent_Identity=43.0894308943089, Blast_Score=688, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 93108; Mature: 93108

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSFNPTTKTQAALTAALQAASAAGNPEIRPAHLLVALLDQTDGIAAPLLKAVGVDPATV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
RREAQDLVDRLPRATGATTQPQLGREALAAITAAQRLATELGDEYVSTEHVLVGLADGDS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
DVTMLLKKYGATADALREAFTTVRGSARVTTPDPEATYQALEKYSTDLTEAARTGKLDPV
HHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDTEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPESLRGKKVISL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHCCCEEEEE
DLGAMVAGAKYRGEFEERLKAVLEDIKNSAGQIITFIDELHTIVGAGATGESAMDAGNMI
EHHHHHHCCHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHH
KPMLARGELRLVGATTLEEYRQHIEKDAALERRFQQVLVGEPSVEDTVGILRGIKERYEV
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HHGVRITDSALVAAAALSDRYITSRFLPDKAIDLVDESASRLRMEIDSRPVEIDEVERAV
HCCCEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RRLEIEEVALSKETDEASKLRLEKLRQELADDREKLNQLMTRWQNEKSAIDQVRTLKEQL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EALRGESERAERDGDLGKAAELRYGRIPSLEKQLAEAEKASGAAADGEVMLKEEVGPDDI
HHHCCCHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHH
AEVVSSWTGIPVGRLLEGETQKLLRMEDELGRRVVGQKEAVQAVSDAVRRARAGVADPNR
HHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCC
PTGSFMFVGPTGVGKTELAKALADFLFDDERAMVRIDMSEYSEKHAVARLVGAPPGYVGY
CCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCC
DQGGQLTEAVRRRPYTVVLFDEIEKAHPDVFDILLQVLDEGRLTDGQGRTVDFRNTILIL
CCCCHHHHHHHHCCCEEEEEHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEECCEEEEE
TSNLGAGGDQEFVMNAVRAAFKPEFLNRLDDVVMFHALDEEQLEHIVDIQLEQLQKRLAQ
EECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RRLTLDVTGSARFWLAVRGYDPVYGARPLRRLIQQAIGDTLAKELLAGTVTDGDTVKVGV
CCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
SPDGDNLVVEPAS
CCCCCCEEEECCC
>Mature Secondary Structure
MDSFNPTTKTQAALTAALQAASAAGNPEIRPAHLLVALLDQTDGIAAPLLKAVGVDPATV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
RREAQDLVDRLPRATGATTQPQLGREALAAITAAQRLATELGDEYVSTEHVLVGLADGDS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
DVTMLLKKYGATADALREAFTTVRGSARVTTPDPEATYQALEKYSTDLTEAARTGKLDPV
HHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDTEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPESLRGKKVISL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHCCCEEEEE
DLGAMVAGAKYRGEFEERLKAVLEDIKNSAGQIITFIDELHTIVGAGATGESAMDAGNMI
EHHHHHHCCHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHH
KPMLARGELRLVGATTLEEYRQHIEKDAALERRFQQVLVGEPSVEDTVGILRGIKERYEV
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HHGVRITDSALVAAAALSDRYITSRFLPDKAIDLVDESASRLRMEIDSRPVEIDEVERAV
HCCCEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RRLEIEEVALSKETDEASKLRLEKLRQELADDREKLNQLMTRWQNEKSAIDQVRTLKEQL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EALRGESERAERDGDLGKAAELRYGRIPSLEKQLAEAEKASGAAADGEVMLKEEVGPDDI
HHHCCCHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHH
AEVVSSWTGIPVGRLLEGETQKLLRMEDELGRRVVGQKEAVQAVSDAVRRARAGVADPNR
HHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCC
PTGSFMFVGPTGVGKTELAKALADFLFDDERAMVRIDMSEYSEKHAVARLVGAPPGYVGY
CCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCC
DQGGQLTEAVRRRPYTVVLFDEIEKAHPDVFDILLQVLDEGRLTDGQGRTVDFRNTILIL
CCCCHHHHHHHHCCCEEEEEHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEECCEEEEE
TSNLGAGGDQEFVMNAVRAAFKPEFLNRLDDVVMFHALDEEQLEHIVDIQLEQLQKRLAQ
EECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RRLTLDVTGSARFWLAVRGYDPVYGARPLRRLIQQAIGDTLAKELLAGTVTDGDTVKVGV
CCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
SPDGDNLVVEPAS
CCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9692182; 12788972 [H]