Click here to switch to the map view.

The map label for this gene is ackA

Identifier: 54027319

GI number: 54027319

Start: 5651600

End: 5652808

Strand: Reverse

Name: ackA

Synonym: nfa53450

Alternate gene names: 54027319

Gene position: 5652808-5651600 (Counterclockwise)

Preceding gene: 54027320

Following gene: 54027315

Centisome position: 93.88

GC content: 71.38

Gene sequence:

>1209_bases
ATGACCGCCGCATCCGAACACCTCGTCCTCGTGCTCAATTCGGGGTCGTCCTCGATCAAGTACCAGCTGGTCGACCCCGA
GACGGGCGAGGTGTCCGCCGCCGGGCTGGTCGAGCGGATCGGTGAGGACGACAGCGCGATCGAGCATCGCTGCGGTGCGC
GCACCATCGGTCACGAGGGCCGCATCCCCGACCACCGCGCGGGCCTGGAGCGGGTGTTCGAGATGTTCGCCGAGACCGGG
TTCGACCTGGCGGGCGCCGGCCTGCGCGCAGTCGGGCACCGGGTGGTGCACGGCGGGGAAATGTTTCACGAGCCCACGCT
GGTGACCGACGAGGTGGTCGCGGCCATCGCCGATTTCGCCGAACTCGCGCCGCTGCACAACCCCGCCAATGTCACCGGCA
TCGAGAACGCGCGTCGCCTGCTACCGGGCGTCCCGCAGGTCGCGGTGTTCGACACCGCCTTCTTCCACGGCCTGCCCGAT
GCCGCCAAGACCTACGCCATCGACGCCAAGATCGCCGCGGAGTACGGCATCCGCCGCTACGGCTTCCACGGCATCTCGCA
CGAGTACGTGTCCGGCCGGGTCGCCGAGGTACTCGACCTCGACCCGGCGCGGCTGCGCCAGATCGTCTTCCACCTGGGCA
ACGGCGCCTCGGCGTCGGCCGTGCGCGGCGGCAGCCCGATCGACACCTCGATGGGCCTGACCCCGCTGGAGGGCCTGGTC
ATGGGCACCAGGGGCGGTGACCTCGATCCCGGCATCCTCGGGCACCTGGCCCGCGCGGCCGGCTTCGACGTCGACCGCAT
CGACAAGCTGCTCAACCGCGAGGCCGGCCTCAAAGGCATGGCGGGCGTCAACGACTTCCGGGAACTGCGCCGCCTGATCG
ACGAGGGCGACGCCGCGGCGAAGCTCGCCTACGACGTCTACGTGCACCGGCTGCGCCGCTACCTCGGCGCCTACCTGATC
GCGCTCGGCGGCGTCGACGCCATCACCTTCACCGCCGGTGTCGGGGAGAACAGCGCCGACGTGCGCGCCGACGCCCTGGC
CGGACTGGAGAACTTCGGCATCGCCGTCGATCCCGCCCGCAACACCGCGAAAGACCGCGGCGCCAGGGTCATTTCGCCGC
CGGCGGCCCCGGTGACGGTGTTGGTGGTGCCGACCAACGAGGAGCTGGCCATCGCCCGCGCGGCCGACCGCCTGGTCGCC
GCGGGCTGA

Upstream 100 bases:

>100_bases
CGGTCAACGATCTCTCCCGTGGCGCGCTGGTCGCCGACATCGTCAACACCGTGGCCATCACCGCCATCCAGGCGCAGGGC
ATCGCGGAACAGGAGGGCTG

Downstream 100 bases:

>100_bases
CGCCGGCCCGCCCGGCGGATCAGAACCAGGTCTTCGCCCGGATGGAGTTGGCCAGGTCGACCAGGGCGTAGCGATGGTCG
CGCCCCGGGGCGGCGCGGGC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTAASEHLVLVLNSGSSSIKYQLVDPETGEVSAAGLVERIGEDDSAIEHRCGARTIGHEGRIPDHRAGLERVFEMFAETG
FDLAGAGLRAVGHRVVHGGEMFHEPTLVTDEVVAAIADFAELAPLHNPANVTGIENARRLLPGVPQVAVFDTAFFHGLPD
AAKTYAIDAKIAAEYGIRRYGFHGISHEYVSGRVAEVLDLDPARLRQIVFHLGNGASASAVRGGSPIDTSMGLTPLEGLV
MGTRGGDLDPGILGHLARAAGFDVDRIDKLLNREAGLKGMAGVNDFRELRRLIDEGDAAAKLAYDVYVHRLRRYLGAYLI
ALGGVDAITFTAGVGENSADVRADALAGLENFGIAVDPARNTAKDRGARVISPPAAPVTVLVVPTNEELAIARAADRLVA
AG

Sequences:

>Translated_402_residues
MTAASEHLVLVLNSGSSSIKYQLVDPETGEVSAAGLVERIGEDDSAIEHRCGARTIGHEGRIPDHRAGLERVFEMFAETG
FDLAGAGLRAVGHRVVHGGEMFHEPTLVTDEVVAAIADFAELAPLHNPANVTGIENARRLLPGVPQVAVFDTAFFHGLPD
AAKTYAIDAKIAAEYGIRRYGFHGISHEYVSGRVAEVLDLDPARLRQIVFHLGNGASASAVRGGSPIDTSMGLTPLEGLV
MGTRGGDLDPGILGHLARAAGFDVDRIDKLLNREAGLKGMAGVNDFRELRRLIDEGDAAAKLAYDVYVHRLRRYLGAYLI
ALGGVDAITFTAGVGENSADVRADALAGLENFGIAVDPARNTAKDRGARVISPPAAPVTVLVVPTNEELAIARAADRLVA
AG
>Mature_401_residues
TAASEHLVLVLNSGSSSIKYQLVDPETGEVSAAGLVERIGEDDSAIEHRCGARTIGHEGRIPDHRAGLERVFEMFAETGF
DLAGAGLRAVGHRVVHGGEMFHEPTLVTDEVVAAIADFAELAPLHNPANVTGIENARRLLPGVPQVAVFDTAFFHGLPDA
AKTYAIDAKIAAEYGIRRYGFHGISHEYVSGRVAEVLDLDPARLRQIVFHLGNGASASAVRGGSPIDTSMGLTPLEGLVM
GTRGGDLDPGILGHLARAAGFDVDRIDKLLNREAGLKGMAGVNDFRELRRLIDEGDAAAKLAYDVYVHRLRRYLGAYLIA
LGGVDAITFTAGVGENSADVRADALAGLENFGIAVDPARNTAKDRGARVISPPAAPVTVLVVPTNEELAIARAADRLVAA
G

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=402, Percent_Identity=43.2835820895522, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI145693184, Length=395, Percent_Identity=40, Blast_Score=276, Evalue=1e-75,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_NOCFA (Q5YNP4)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121561.1
- ProteinModelPortal:   Q5YNP4
- SMR:   Q5YNP4
- GeneID:   3109913
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa53450
- NMPDR:   fig|247156.1.peg.5142
- HOGENOM:   HBG285863
- OMA:   HGGDLFT
- ProtClustDB:   PRK00180
- BioCyc:   NFAR247156:NFA53450-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 42395; Mature: 42264

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAASEHLVLVLNSGSSSIKYQLVDPETGEVSAAGLVERIGEDDSAIEHRCGARTIGHEG
CCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCC
RIPDHRAGLERVFEMFAETGFDLAGAGLRAVGHRVVHGGEMFHEPTLVTDEVVAAIADFA
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHH
ELAPLHNPANVTGIENARRLLPGVPQVAVFDTAFFHGLPDAAKTYAIDAKIAAEYGIRRY
HHCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHEEEHHHHHHHHCHHHH
GFHGISHEYVSGRVAEVLDLDPARLRQIVFHLGNGASASAVRGGSPIDTSMGLTPLEGLV
CCCCCCHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHEE
MGTRGGDLDPGILGHLARAAGFDVDRIDKLLNREAGLKGMAGVNDFRELRRLIDEGDAAA
EECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHH
KLAYDVYVHRLRRYLGAYLIALGGVDAITFTAGVGENSADVRADALAGLENFGIAVDPAR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEECCCC
NTAKDRGARVISPPAAPVTVLVVPTNEELAIARAADRLVAAG
CCHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TAASEHLVLVLNSGSSSIKYQLVDPETGEVSAAGLVERIGEDDSAIEHRCGARTIGHEG
CCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCC
RIPDHRAGLERVFEMFAETGFDLAGAGLRAVGHRVVHGGEMFHEPTLVTDEVVAAIADFA
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHH
ELAPLHNPANVTGIENARRLLPGVPQVAVFDTAFFHGLPDAAKTYAIDAKIAAEYGIRRY
HHCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHEEEHHHHHHHHCHHHH
GFHGISHEYVSGRVAEVLDLDPARLRQIVFHLGNGASASAVRGGSPIDTSMGLTPLEGLV
CCCCCCHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHEE
MGTRGGDLDPGILGHLARAAGFDVDRIDKLLNREAGLKGMAGVNDFRELRRLIDEGDAAA
EECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHH
KLAYDVYVHRLRRYLGAYLIALGGVDAITFTAGVGENSADVRADALAGLENFGIAVDPAR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEECCCC
NTAKDRGARVISPPAAPVTVLVVPTNEELAIARAADRLVAAG
CCHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA