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The map label for this gene is thiO/thiG [H]

Identifier: 54027313

GI number: 54027313

Start: 5643678

End: 5644781

Strand: Reverse

Name: thiO/thiG [H]

Synonym: nfa53390

Alternate gene names: 54027313

Gene position: 5644781-5643678 (Counterclockwise)

Preceding gene: 54027315

Following gene: 54027312

Centisome position: 93.75

GC content: 76.63

Gene sequence:

>1104_bases
GTGGCAGACATGCGGACTCTGGCCGTCGTCGGCGGCGGCGCCATCGGTTTCGCGGTGGCCTGGCGGGCCGCCGAAGCGGG
ATGGCGGGTCACGCTGTTCGACGCCGCCGCCGGGTCCGGCGCCTCCCGGGTCGCGGGTGGCATGCTCGCGCCGCTGTCGG
AGGGCTGGCCGGGGGAGGACCGGGTCCTCGAGTTCGGCGCCGCCTCGCTGGCGCGCTGGCCCGATTTCGCCGCTCGGCTG
CGCAGCGCCACCGGCATCGATGTCTTCGTCGCCGAGGCGACCCTGACCGTCGCGCTCGACGCCGCCGACGCGGCCGACCT
GCGCACCGTCGCCGACTGGGTCGGCGAACGCGGCCACGACCTGCGCGTGCTGGATCGGGCGGGCGTCCGCGCGATCGAAC
CCGCCCTCGCCCGGACCGTGCGCGCGGGGATGCTCTCCGCCGAACCCGCCATCGACAATCGGCAGCTGTTCCGGGCACTG
CACGCCGCGGCCACGGCGGCCGGGGTGGACGTGCGCGCCGAGTCCGTCACCGACCTGACGGCCCTGTCCTTCGACCAGGT
GGTGCTGGCCGCGGGCGCCGCGTCCGCCGGGTTGTGGCCGGGCCTGCCGGTGCGGCCGGTGAAGGGCGAGATCCTGCGCC
TGCGGCACCGGCCGGGCGCGGCGCCCGCACCCCGCCGGGTGGTGCGTGCCCGGGTGCACGGCCGCCCGGTCTACCTGGTG
CCGCGCGCGGACGGCATCGTCGTCGGCGCCACCCAGTACGAAGCCGGGTTCGACACCACCGTCACCGTCGGCGGTGTCCG
TGATCTGATCGCCGACGCCGAGGCGGTGCTGCCCGGCATCGGTGAATACGAGCTGGCCGAGGCGAGCGCGGGCTCCCGCC
CCGGCACCCCGGACAACCTGCCTCTCATCGGCAGGCTGTCCGAGCGGGTCGTGGCCGCCACCGGCCACGGCCGCAACGGC
ATGCTGGCCGTGCCGCTCACCGTCGACGCGACGATGGCCGCGCTCGGTGACGCGCCGCTGCCCGAGGCCGCGGCCGCCGA
CCCGCACCGCTTCACCCCGACGCCCGCCCCGGCGTCGCTGTCCGCGTCAGGAGGAATTCGATGA

Upstream 100 bases:

>100_bases
TGGCAGCGGTCGCTTCGGGTGGGAGGGATGCACGTCGTCGACGGTAGCCGCGCTACGGTGGGGAAGCGAGCAGAGGGGGA
CCCGAGCGCTATCCAGGAAG

Downstream 100 bases:

>100_bases
CCGCACCATCCATCCCCATCGGTGTCACGGTGAACGGCGAGGACCACGAGTTCGCCGAGCCGCTCACCGTGCGGGAACTG
CTGGACCGGCTCGCGCTGCC

Product: putative amino acid oxidase

Products: NA

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MADMRTLAVVGGGAIGFAVAWRAAEAGWRVTLFDAAAGSGASRVAGGMLAPLSEGWPGEDRVLEFGAASLARWPDFAARL
RSATGIDVFVAEATLTVALDAADAADLRTVADWVGERGHDLRVLDRAGVRAIEPALARTVRAGMLSAEPAIDNRQLFRAL
HAAATAAGVDVRAESVTDLTALSFDQVVLAAGAASAGLWPGLPVRPVKGEILRLRHRPGAAPAPRRVVRARVHGRPVYLV
PRADGIVVGATQYEAGFDTTVTVGGVRDLIADAEAVLPGIGEYELAEASAGSRPGTPDNLPLIGRLSERVVAATGHGRNG
MLAVPLTVDATMAALGDAPLPEAAAADPHRFTPTPAPASLSASGGIR

Sequences:

>Translated_367_residues
MADMRTLAVVGGGAIGFAVAWRAAEAGWRVTLFDAAAGSGASRVAGGMLAPLSEGWPGEDRVLEFGAASLARWPDFAARL
RSATGIDVFVAEATLTVALDAADAADLRTVADWVGERGHDLRVLDRAGVRAIEPALARTVRAGMLSAEPAIDNRQLFRAL
HAAATAAGVDVRAESVTDLTALSFDQVVLAAGAASAGLWPGLPVRPVKGEILRLRHRPGAAPAPRRVVRARVHGRPVYLV
PRADGIVVGATQYEAGFDTTVTVGGVRDLIADAEAVLPGIGEYELAEASAGSRPGTPDNLPLIGRLSERVVAATGHGRNG
MLAVPLTVDATMAALGDAPLPEAAAADPHRFTPTPAPASLSASGGIR
>Mature_366_residues
ADMRTLAVVGGGAIGFAVAWRAAEAGWRVTLFDAAAGSGASRVAGGMLAPLSEGWPGEDRVLEFGAASLARWPDFAARLR
SATGIDVFVAEATLTVALDAADAADLRTVADWVGERGHDLRVLDRAGVRAIEPALARTVRAGMLSAEPAIDNRQLFRALH
AAATAAGVDVRAESVTDLTALSFDQVVLAAGAASAGLWPGLPVRPVKGEILRLRHRPGAAPAPRRVVRARVHGRPVYLVP
RADGIVVGATQYEAGFDTTVTVGGVRDLIADAEAVLPGIGEYELAEASAGSRPGTPDNLPLIGRLSERVVAATGHGRNGM
LAVPLTVDATMAALGDAPLPEAAAADPHRFTPTPAPASLSASGGIR

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 37656; Mature: 37525

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADMRTLAVVGGGAIGFAVAWRAAEAGWRVTLFDAAAGSGASRVAGGMLAPLSEGWPGED
CCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCEECCCCCCCCCHH
RVLEFGAASLARWPDFAARLRSATGIDVFVAEATLTVALDAADAADLRTVADWVGERGHD
HHHHHCHHHHHHCHHHHHHHHCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCC
LRVLDRAGVRAIEPALARTVRAGMLSAEPAIDNRQLFRALHAAATAAGVDVRAESVTDLT
EEEEHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHH
ALSFDQVVLAAGAASAGLWPGLPVRPVKGEILRLRHRPGAAPAPRRVVRARVHGRPVYLV
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEE
PRADGIVVGATQYEAGFDTTVTVGGVRDLIADAEAVLPGIGEYELAEASAGSRPGTPDNL
ECCCCEEEEEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCC
PLIGRLSERVVAATGHGRNGMLAVPLTVDATMAALGDAPLPEAAAADPHRFTPTPAPASL
CCCCCCCCEEEEECCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
SASGGIR
CCCCCCC
>Mature Secondary Structure 
ADMRTLAVVGGGAIGFAVAWRAAEAGWRVTLFDAAAGSGASRVAGGMLAPLSEGWPGED
CCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCEECCCCCCCCCHH
RVLEFGAASLARWPDFAARLRSATGIDVFVAEATLTVALDAADAADLRTVADWVGERGHD
HHHHHCHHHHHHCHHHHHHHHCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCC
LRVLDRAGVRAIEPALARTVRAGMLSAEPAIDNRQLFRALHAAATAAGVDVRAESVTDLT
EEEEHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHH
ALSFDQVVLAAGAASAGLWPGLPVRPVKGEILRLRHRPGAAPAPRRVVRARVHGRPVYLV
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEE
PRADGIVVGATQYEAGFDTTVTVGGVRDLIADAEAVLPGIGEYELAEASAGSRPGTPDNL
ECCCCEEEEEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCC
PLIGRLSERVVAATGHGRNGMLAVPLTVDATMAALGDAPLPEAAAADPHRFTPTPAPASL
CCCCCCCCEEEEECCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
SASGGIR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]