Click here to switch to the map view.

The map label for this gene is thiG

Identifier: 54027311

GI number: 54027311

Start: 5642700

End: 5643470

Strand: Reverse

Name: thiG

Synonym: nfa53370

Alternate gene names: 54027311

Gene position: 5643470-5642700 (Counterclockwise)

Preceding gene: 54027312

Following gene: 54027310

Centisome position: 93.73

GC content: 72.89

Gene sequence:

>771_bases
GTGGCTGAGCATCCCGACCTGCCGCCGCTGCGCATCGCCGACCGGGAGTTCGGTTCCCGCCTCATCATGGGCACCGGCGG
TGCCGAGAACCTGGCCGTGCTGGAGGAGGCCCTGGTCGCCTCCGGCACCGAACTCACCACGGTCGCCATGCGCCGCGTCG
ACGCCGCGGGCGGCACGGGCGTGCTCGACCTGCTGAAACGGCTCGAGATCACCCCGTTGCCGAACACCGCGGGCTGCCGG
ACCGCCGCCGAGGCGGTGTTGACCGCGCAGCTGGCCGCCGAAGCGCTCGACACCACCTGGGTCAAGCTCGAGGTCGTCGC
CGACGAACGCACCCTGCTTCCGGACCCGATCGAATTGCTCAGCGCGGCAGAGCAATTGGTCGATGCCGGTTTCACCGTGC
TGCCCTACACCAACGACGACCCGGTGCTGGCCCGGCGGCTGGAGGACGCGGGCTGCGCGGCGGTGATGCCGCTGGGCGCC
CCCATCGGCACCGGCCTCGGCATCGGCAACCCGCACAACATCGAGATGATCGTCGCCGCCGCGGGCGTGCCGGTGATCCT
CGACGCGGGCATCGGCACCGCGAGCGATGCCGCGCTGGCGATGGAACTCGGCTGCTCGGCGGTGCTGCTGGCCACCGCCG
TGACCCGGGCCAAGCGCCCGCCGCTGATGGCGGCGGCGATGGCCGACGCCGTACGGGCGGGTCTGCTCGCCCGCGAGGCG
GGCCGCATCCCCAAGCGGTTCTGGGCTCAGGCGTCCTCCCCGCAGCGGTAA

Upstream 100 bases:

>100_bases
GGCGTCGCGGTGGCCGTGGACGGCGCGGTGTTCCCGAAGTCGCGCTGGGACGAACAGGTCGGGCGCGGCTGGTCCATCGA
GGTGCTGACGGCGGTCCAAG

Downstream 100 bases:

>100_bases
GGGCCGCAGCGGTAGGGGCAGGCCCCCGGGGCACTGGGGGATCGGGTCATTCTCGAGGATGACCGAATTCCACCCCAGGT
GGGGACGCGCGCGGGCCCGT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAEHPDLPPLRIADREFGSRLIMGTGGAENLAVLEEALVASGTELTTVAMRRVDAAGGTGVLDLLKRLEITPLPNTAGCR
TAAEAVLTAQLAAEALDTTWVKLEVVADERTLLPDPIELLSAAEQLVDAGFTVLPYTNDDPVLARRLEDAGCAAVMPLGA
PIGTGLGIGNPHNIEMIVAAAGVPVILDAGIGTASDAALAMELGCSAVLLATAVTRAKRPPLMAAAMADAVRAGLLAREA
GRIPKRFWAQASSPQR

Sequences:

>Translated_256_residues
MAEHPDLPPLRIADREFGSRLIMGTGGAENLAVLEEALVASGTELTTVAMRRVDAAGGTGVLDLLKRLEITPLPNTAGCR
TAAEAVLTAQLAAEALDTTWVKLEVVADERTLLPDPIELLSAAEQLVDAGFTVLPYTNDDPVLARRLEDAGCAAVMPLGA
PIGTGLGIGNPHNIEMIVAAAGVPVILDAGIGTASDAALAMELGCSAVLLATAVTRAKRPPLMAAAMADAVRAGLLAREA
GRIPKRFWAQASSPQR
>Mature_255_residues
AEHPDLPPLRIADREFGSRLIMGTGGAENLAVLEEALVASGTELTTVAMRRVDAAGGTGVLDLLKRLEITPLPNTAGCRT
AAEAVLTAQLAAEALDTTWVKLEVVADERTLLPDPIELLSAAEQLVDAGFTVLPYTNDDPVLARRLEDAGCAAVMPLGAP
IGTGLGIGNPHNIEMIVAAAGVPVILDAGIGTASDAALAMELGCSAVLLATAVTRAKRPPLMAAAMADAVRAGLLAREAG
RIPKRFWAQASSPQR

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=246, Percent_Identity=52.0325203252033, Blast_Score=230, Evalue=8e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_NOCFA (Q5YNQ2)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121553.1
- ProteinModelPortal:   Q5YNQ2
- SMR:   Q5YNQ2
- GeneID:   3111588
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa53370
- NMPDR:   fig|247156.1.peg.5134
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   NFAR247156:NFA53370-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 26409; Mature: 26278

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: ACT_SITE 102-102 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHPDLPPLRIADREFGSRLIMGTGGAENLAVLEEALVASGTELTTVAMRRVDAAGGTG
CCCCCCCCCCEECCHHHCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
VLDLLKRLEITPLPNTAGCRTAAEAVLTAQLAAEALDTTWVKLEVVADERTLLPDPIELL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHH
SAAEQLVDAGFTVLPYTNDDPVLARRLEDAGCAAVMPLGAPIGTGLGIGNPHNIEMIVAA
HHHHHHHHCCCEEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEE
AGVPVILDAGIGTASDAALAMELGCSAVLLATAVTRAKRPPLMAAAMADAVRAGLLAREA
CCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GRIPKRFWAQASSPQR
CCCCHHHHHCCCCCCC
>Mature Secondary Structure 
AEHPDLPPLRIADREFGSRLIMGTGGAENLAVLEEALVASGTELTTVAMRRVDAAGGTG
CCCCCCCCCEECCHHHCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
VLDLLKRLEITPLPNTAGCRTAAEAVLTAQLAAEALDTTWVKLEVVADERTLLPDPIELL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHH
SAAEQLVDAGFTVLPYTNDDPVLARRLEDAGCAAVMPLGAPIGTGLGIGNPHNIEMIVAA
HHHHHHHHCCCEEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEE
AGVPVILDAGIGTASDAALAMELGCSAVLLATAVTRAKRPPLMAAAMADAVRAGLLAREA
CCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GRIPKRFWAQASSPQR
CCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA