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The map label for this gene is regX3 [H]

Identifier: 54027160

GI number: 54027160

Start: 5483440

End: 5484129

Strand: Reverse

Name: regX3 [H]

Synonym: nfa51860

Alternate gene names: 54027160

Gene position: 5484129-5483440 (Counterclockwise)

Preceding gene: 54027161

Following gene: 54027155

Centisome position: 91.08

GC content: 68.26

Gene sequence:

>690_bases
ATGACGAGTGTTCTGATCGTCGAGGATGAGGAGTCGCTGGCCGATCCGCTCGCGTTCCTGCTGCGCAAGGAGGGCTTCGA
GGTCACCGTGGTGGGCGACGGTCCGTCGGCGCTGGCCGAATTCGACCGCTCCGGCGCCGACATCGTGCTGCTCGATCTCA
TGCTGCCGGGCATGAGCGGCACGGATGTCTGCAAGCAGCTGCGCACCCGCAGCGGGGTACCGGTCATCATGGTGACCGCG
CGCGACAGCGAGATCGACAAGGTGGTCGGCCTCGAGCTGGGTGCCGACGACTACGTCACCAAGCCGTACTCGGCGCGGGA
GCTGATCGCCCGCATCCGGGCCGTGCTGCGCCGCGGCGCGGGCGACGAGCTCGACGGCAACGGCGAGAGCGGCGTGCTCG
AGGCCGGTCCGGTGCGGATGGACGTCGACCGGCACACCGTGATGGTGAACGGCAAACCGGTGACGTTGCCGCTCAAGGAG
TTCGACCTGCTCGAGTACCTGCTGCGCAACTCCGGCCGGGTGCTGACCCGCGGCCAGCTGATCGATCGGGTGTGGGGCGC
GGACTACGTCGGCGACACCAAGACCCTCGATGTGCACGTCAAGCGACTGCGCTCGAAGATCGAAGCCGACCCCGCCAAGC
CGGAGCACCTGGTCACCGTCCGCGGCCTGGGGTACAAGCTCGAAGCCTGA

Upstream 100 bases:

>100_bases
GCGACGACGACGTCGACGCGGACGGCGCCGCGGTGAGCACGAAGGAAAACGGCTCGCGTCCCTCGGGGCCGGGCAGACCC
AATGGTGTGGAGGCACGCAG

Downstream 100 bases:

>100_bases
GCGGGTATGGCCGTCGTGCTGTCTGCCGTTCGCGTGGTTCAGGGCAGGTAGATCCAGGTGGGGCCGATGCCGGGAACGTC
GGTGAGGCGCAGGGTGATCC

Product: putative two-component system response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTSVLIVEDEESLADPLAFLLRKEGFEVTVVGDGPSALAEFDRSGADIVLLDLMLPGMSGTDVCKQLRTRSGVPVIMVTA
RDSEIDKVVGLELGADDYVTKPYSARELIARIRAVLRRGAGDELDGNGESGVLEAGPVRMDVDRHTVMVNGKPVTLPLKE
FDLLEYLLRNSGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRSKIEADPAKPEHLVTVRGLGYKLEA

Sequences:

>Translated_229_residues
MTSVLIVEDEESLADPLAFLLRKEGFEVTVVGDGPSALAEFDRSGADIVLLDLMLPGMSGTDVCKQLRTRSGVPVIMVTA
RDSEIDKVVGLELGADDYVTKPYSARELIARIRAVLRRGAGDELDGNGESGVLEAGPVRMDVDRHTVMVNGKPVTLPLKE
FDLLEYLLRNSGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRSKIEADPAKPEHLVTVRGLGYKLEA
>Mature_228_residues
TSVLIVEDEESLADPLAFLLRKEGFEVTVVGDGPSALAEFDRSGADIVLLDLMLPGMSGTDVCKQLRTRSGVPVIMVTAR
DSEIDKVVGLELGADDYVTKPYSARELIARIRAVLRRGAGDELDGNGESGVLEAGPVRMDVDRHTVMVNGKPVTLPLKEF
DLLEYLLRNSGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRSKIEADPAKPEHLVTVRGLGYKLEA

Specific function: Part of the two-component regulatory system senX3/regX3. Once phosphorylated by senX3, activates the expression of several operons/genes involved in phosphate assimilation and metabolism, such as pstSCAB, phnDCE, and phoA [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=39.4736842105263, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1789809, Length=226, Percent_Identity=38.9380530973451, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=38.3259911894273, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=38.4955752212389, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1786784, Length=227, Percent_Identity=38.7665198237885, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI87082012, Length=226, Percent_Identity=36.7256637168142, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI1786911, Length=227, Percent_Identity=37.4449339207048, Blast_Score=146, Evalue=9e-37,
Organism=Escherichia coli, GI2367329, Length=227, Percent_Identity=39.2070484581498, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=36.8888888888889, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=32.9004329004329, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=36.2445414847162, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1787375, Length=226, Percent_Identity=33.6283185840708, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1790552, Length=228, Percent_Identity=34.6491228070175, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=30.0429184549356, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788550, Length=159, Percent_Identity=29.559748427673, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25010; Mature: 24879

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVLIVEDEESLADPLAFLLRKEGFEVTVVGDGPSALAEFDRSGADIVLLDLMLPGMSG
CCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCEEEEEEECCCCCH
TDVCKQLRTRSGVPVIMVTARDSEIDKVVGLELGADDYVTKPYSARELIARIRAVLRRGA
HHHHHHHHHCCCCCEEEEEECCCCCHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
GDELDGNGESGVLEAGPVRMDVDRHTVMVNGKPVTLPLKEFDLLEYLLRNSGRVLTRGQL
CCCCCCCCCCCCEECCCEEEECCCEEEEECCCEEEEEHHHHHHHHHHHHCCCCEEEHHHH
IDRVWGADYVGDTKTLDVHVKRLRSKIEADPAKPEHLVTVRGLGYKLEA
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEECC
>Mature Secondary Structure 
TSVLIVEDEESLADPLAFLLRKEGFEVTVVGDGPSALAEFDRSGADIVLLDLMLPGMSG
CEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCEEEEEEECCCCCH
TDVCKQLRTRSGVPVIMVTARDSEIDKVVGLELGADDYVTKPYSARELIARIRAVLRRGA
HHHHHHHHHCCCCCEEEEEECCCCCHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
GDELDGNGESGVLEAGPVRMDVDRHTVMVNGKPVTLPLKEFDLLEYLLRNSGRVLTRGQL
CCCCCCCCCCCCEECCCEEEECCCEEEEECCCEEEEEHHHHHHHHHHHHCCCCEEEHHHH
IDRVWGADYVGDTKTLDVHVKRLRSKIEADPAKPEHLVTVRGLGYKLEA
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA