| Definition | Nocardia farcinica IFM 10152 chromosome, complete genome. |
|---|---|
| Accession | NC_006361 |
| Length | 6,021,225 |
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The map label for this gene is hdhA [C]
Identifier: 54026635
GI number: 54026635
Start: 4860822
End: 4861571
Strand: Reverse
Name: hdhA [C]
Synonym: nfa46620
Alternate gene names: 54026635
Gene position: 4861571-4860822 (Counterclockwise)
Preceding gene: 54026636
Following gene: 54026634
Centisome position: 80.74
GC content: 68.13
Gene sequence:
>750_bases ATGGGGCGTTTCACCGACCGAACCGCCGTCGTCACCGGCGCCGCCCAGGGGATCGGCGCGGCCTATGCCGCCGCGCTCGC CGCCGAGGGCGCCGACGTGGTCGTGGCCGACCGCAACATCGACGCGGGCAAGACCGTCGCCGCCGAGATCACCGCCTCCG GCGGCAGCGCGGTGTTCTGCGAGGTCGACGTCGCCGATCCCGATTCCGCCACCGCGCTGGCCGAGACCACGATGCAGCGC TTCGGCCGTATCGACCACCTGGTCAACAACGCCGCCATCTACGGCGACATGAAGCTGGACCTGCTCATCACCGTGCCCTG GGACTACTACAAGAAGTTCATGTCGGTGAACATGGACGGCGCACTCAACGTGACCCGGGCGGTGTACCCGCACATGCAGC GGTCGGGCGGCTCGATCGTGAACCAGTCCTCCACCGCGGCCTGGGTCTACTCCGGGTTCTACGGTGTGGCCAAGGCGGGC CTCAACAGCCTCACCCAGCAACTCGCGGTCGAACTCGGCGGCAGCAACATCCGCGTCAACGCCATCGCGCCCGGGCCCAT CGACACCGACGCCACCAAGACCGTCACCCCCGGCAGCATCGTGGCCGACATGGTGAAGCGGCTGCCGCTCAAGCGGATGG GCACGCCGCAGGACCTCGTCGGGGCCTGCCTCTACCTGCTCTCCGACGAGGCATCGTGGGTGACCGGGCAGATCTTCAAC ATCGACGGCGGACAGATCATCCGGTCATGA
Upstream 100 bases:
>100_bases CGAGATGGGCACCGCGGGCTTCGAGGAATACCTGGAGACCAAGCTGATCGCCACCGCGGTCTGAACCCCTTCCCACCCAA CGACTTCCGAGGAGCCCACC
Downstream 100 bases:
>100_bases GCGACATCCGGGTCGGATTCATCGGATTGGGCAACATGGGCGCGCCCATGGCACAGCGCCTGCTGGCCTGGCCGGGCGGG CTCACCGTCTGCGACACGCG
Product: short chain dehydrogenase
Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]
Alternate protein names: ORFC [H]
Number of amino acids: Translated: 249; Mature: 248
Protein sequence:
>249_residues MGRFTDRTAVVTGAAQGIGAAYAAALAAEGADVVVADRNIDAGKTVAAEITASGGSAVFCEVDVADPDSATALAETTMQR FGRIDHLVNNAAIYGDMKLDLLITVPWDYYKKFMSVNMDGALNVTRAVYPHMQRSGGSIVNQSSTAAWVYSGFYGVAKAG LNSLTQQLAVELGGSNIRVNAIAPGPIDTDATKTVTPGSIVADMVKRLPLKRMGTPQDLVGACLYLLSDEASWVTGQIFN IDGGQIIRS
Sequences:
>Translated_249_residues MGRFTDRTAVVTGAAQGIGAAYAAALAAEGADVVVADRNIDAGKTVAAEITASGGSAVFCEVDVADPDSATALAETTMQR FGRIDHLVNNAAIYGDMKLDLLITVPWDYYKKFMSVNMDGALNVTRAVYPHMQRSGGSIVNQSSTAAWVYSGFYGVAKAG LNSLTQQLAVELGGSNIRVNAIAPGPIDTDATKTVTPGSIVADMVKRLPLKRMGTPQDLVGACLYLLSDEASWVTGQIFN IDGGQIIRS >Mature_248_residues GRFTDRTAVVTGAAQGIGAAYAAALAAEGADVVVADRNIDAGKTVAAEITASGGSAVFCEVDVADPDSATALAETTMQRF GRIDHLVNNAAIYGDMKLDLLITVPWDYYKKFMSVNMDGALNVTRAVYPHMQRSGGSIVNQSSTAAWVYSGFYGVAKAGL NSLTQQLAVELGGSNIRVNAIAPGPIDTDATKTVTPGSIVADMVKRLPLKRMGTPQDLVGACLYLLSDEASWVTGQIFNI DGGQIIRS
Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI10190704, Length=249, Percent_Identity=34.5381526104418, Blast_Score=119, Evalue=3e-27, Organism=Homo sapiens, GI32483357, Length=249, Percent_Identity=35.7429718875502, Blast_Score=118, Evalue=5e-27, Organism=Homo sapiens, GI19923817, Length=256, Percent_Identity=31.25, Blast_Score=116, Evalue=2e-26, Organism=Homo sapiens, GI4504505, Length=257, Percent_Identity=33.0739299610895, Blast_Score=104, Evalue=8e-23, Organism=Homo sapiens, GI40254992, Length=247, Percent_Identity=31.9838056680162, Blast_Score=103, Evalue=1e-22, Organism=Homo sapiens, GI5031737, Length=247, Percent_Identity=31.5789473684211, Blast_Score=103, Evalue=2e-22, Organism=Homo sapiens, GI15277342, Length=255, Percent_Identity=31.7647058823529, Blast_Score=102, Evalue=5e-22, Organism=Homo sapiens, GI4503301, Length=250, Percent_Identity=30, Blast_Score=97, Evalue=1e-20, Organism=Homo sapiens, GI126723750, Length=249, Percent_Identity=32.1285140562249, Blast_Score=96, Evalue=3e-20, Organism=Homo sapiens, GI59889578, Length=228, Percent_Identity=28.0701754385965, Blast_Score=92, Evalue=5e-19, Organism=Homo sapiens, GI33667109, Length=201, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-18, Organism=Homo sapiens, GI7705925, Length=244, Percent_Identity=31.5573770491803, Blast_Score=89, Evalue=5e-18, Organism=Homo sapiens, GI31542939, Length=200, Percent_Identity=31, Blast_Score=84, Evalue=2e-16, Organism=Homo sapiens, GI40254986, Length=196, Percent_Identity=33.1632653061224, Blast_Score=82, Evalue=4e-16, Organism=Homo sapiens, GI7706318, Length=206, Percent_Identity=29.6116504854369, Blast_Score=82, Evalue=6e-16, Organism=Homo sapiens, GI126723191, Length=186, Percent_Identity=34.9462365591398, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI4758504, Length=252, Percent_Identity=31.7460317460317, Blast_Score=79, Evalue=5e-15, Organism=Homo sapiens, GI109715829, Length=253, Percent_Identity=28.4584980237154, Blast_Score=77, Evalue=1e-14, Organism=Homo sapiens, GI66933014, Length=256, Percent_Identity=31.640625, Blast_Score=77, Evalue=2e-14, Organism=Homo sapiens, GI20149619, Length=192, Percent_Identity=32.2916666666667, Blast_Score=74, Evalue=1e-13, Organism=Homo sapiens, GI224922801, Length=146, Percent_Identity=32.1917808219178, Blast_Score=68, Evalue=8e-12, Organism=Homo sapiens, GI83715985, Length=254, Percent_Identity=29.5275590551181, Blast_Score=65, Evalue=4e-11, Organism=Escherichia coli, GI1787905, Length=246, Percent_Identity=36.5853658536585, Blast_Score=154, Evalue=7e-39, Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=34.7490347490347, Blast_Score=133, Evalue=8e-33, Organism=Escherichia coli, GI1787335, Length=245, Percent_Identity=31.8367346938775, Blast_Score=119, Evalue=2e-28, Organism=Escherichia coli, GI1790717, Length=246, Percent_Identity=30.8943089430894, Blast_Score=114, Evalue=4e-27, Organism=Escherichia coli, GI1788459, Length=245, Percent_Identity=32.6530612244898, Blast_Score=107, Evalue=6e-25, Organism=Escherichia coli, GI87082160, Length=247, Percent_Identity=31.5789473684211, Blast_Score=107, Evalue=1e-24, Organism=Escherichia coli, GI2367175, Length=249, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=1e-23, Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=31.0483870967742, Blast_Score=100, Evalue=1e-22, Organism=Escherichia coli, GI1786812, Length=258, Percent_Identity=30.6201550387597, Blast_Score=96, Evalue=2e-21, Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=29.6442687747036, Blast_Score=94, Evalue=7e-21, Organism=Escherichia coli, GI1789057, Length=260, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=2e-19, Organism=Escherichia coli, GI2367365, Length=252, Percent_Identity=30.5555555555556, Blast_Score=88, Evalue=4e-19, Organism=Escherichia coli, GI1787526, Length=252, Percent_Identity=26.984126984127, Blast_Score=70, Evalue=1e-13, Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=25.6809338521401, Blast_Score=66, Evalue=2e-12, Organism=Escherichia coli, GI1787820, Length=180, Percent_Identity=28.8888888888889, Blast_Score=65, Evalue=6e-12, Organism=Caenorhabditis elegans, GI17562910, Length=264, Percent_Identity=33.7121212121212, Blast_Score=141, Evalue=4e-34, Organism=Caenorhabditis elegans, GI17560676, Length=260, Percent_Identity=36.5384615384615, Blast_Score=130, Evalue=9e-31, Organism=Caenorhabditis elegans, GI17561402, Length=254, Percent_Identity=35.8267716535433, Blast_Score=129, Evalue=2e-30, Organism=Caenorhabditis elegans, GI71994600, Length=249, Percent_Identity=30.1204819277108, Blast_Score=120, Evalue=8e-28, Organism=Caenorhabditis elegans, GI17560332, Length=262, Percent_Identity=31.2977099236641, Blast_Score=114, Evalue=4e-26, Organism=Caenorhabditis elegans, GI17562906, Length=264, Percent_Identity=30.6818181818182, Blast_Score=114, Evalue=6e-26, Organism=Caenorhabditis elegans, GI71994604, Length=248, Percent_Identity=29.0322580645161, Blast_Score=114, Evalue=7e-26, Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=35.8870967741936, Blast_Score=112, Evalue=2e-25, Organism=Caenorhabditis elegans, GI17555706, Length=247, Percent_Identity=32.3886639676113, Blast_Score=111, Evalue=3e-25, Organism=Caenorhabditis elegans, GI17562904, Length=265, Percent_Identity=30.9433962264151, Blast_Score=110, Evalue=5e-25, Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=30.4182509505703, Blast_Score=110, Evalue=8e-25, Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=29.1666666666667, Blast_Score=108, Evalue=2e-24, Organism=Caenorhabditis elegans, GI17560150, Length=263, Percent_Identity=29.6577946768061, Blast_Score=107, Evalue=5e-24, Organism=Caenorhabditis elegans, GI17538480, Length=261, Percent_Identity=29.8850574712644, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI17551412, Length=261, Percent_Identity=30.2681992337165, Blast_Score=104, Evalue=5e-23, Organism=Caenorhabditis elegans, GI17538486, Length=262, Percent_Identity=29.7709923664122, Blast_Score=103, Evalue=6e-23, Organism=Caenorhabditis elegans, GI17531453, Length=261, Percent_Identity=29.8850574712644, Blast_Score=103, Evalue=8e-23, Organism=Caenorhabditis elegans, GI17536651, Length=255, Percent_Identity=30.9803921568627, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI193204405, Length=266, Percent_Identity=30.0751879699248, Blast_Score=100, Evalue=7e-22, Organism=Caenorhabditis elegans, GI17536025, Length=257, Percent_Identity=32.295719844358, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17560220, Length=267, Percent_Identity=29.9625468164794, Blast_Score=98, Evalue=5e-21, Organism=Caenorhabditis elegans, GI17565030, Length=262, Percent_Identity=29.3893129770992, Blast_Score=97, Evalue=6e-21, Organism=Caenorhabditis elegans, GI115534694, Length=247, Percent_Identity=33.1983805668016, Blast_Score=97, Evalue=9e-21, Organism=Caenorhabditis elegans, GI17562990, Length=263, Percent_Identity=28.5171102661597, Blast_Score=96, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17544670, Length=268, Percent_Identity=26.865671641791, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=26.615969581749, Blast_Score=95, Evalue=3e-20, Organism=Caenorhabditis elegans, GI17563726, Length=265, Percent_Identity=28.3018867924528, Blast_Score=94, Evalue=7e-20, Organism=Caenorhabditis elegans, GI17568967, Length=215, Percent_Identity=34.8837209302326, Blast_Score=91, Evalue=4e-19, Organism=Caenorhabditis elegans, GI17561272, Length=239, Percent_Identity=30.5439330543933, Blast_Score=91, Evalue=7e-19, Organism=Caenorhabditis elegans, GI71982365, Length=197, Percent_Identity=31.9796954314721, Blast_Score=79, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17538182, Length=249, Percent_Identity=28.1124497991968, Blast_Score=77, Evalue=6e-15, Organism=Caenorhabditis elegans, GI17558628, Length=199, Percent_Identity=32.1608040201005, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17567345, Length=166, Percent_Identity=30.1204819277108, Blast_Score=70, Evalue=1e-12, Organism=Saccharomyces cerevisiae, GI6324126, Length=249, Percent_Identity=31.3253012048193, Blast_Score=114, Evalue=2e-26, Organism=Saccharomyces cerevisiae, GI6322861, Length=255, Percent_Identity=33.7254901960784, Blast_Score=106, Evalue=4e-24, Organism=Saccharomyces cerevisiae, GI6323882, Length=197, Percent_Identity=28.9340101522843, Blast_Score=66, Evalue=6e-12, Organism=Drosophila melanogaster, GI23397609, Length=254, Percent_Identity=34.6456692913386, Blast_Score=129, Evalue=2e-30, Organism=Drosophila melanogaster, GI24639444, Length=250, Percent_Identity=33.6, Blast_Score=117, Evalue=6e-27, Organism=Drosophila melanogaster, GI21357041, Length=257, Percent_Identity=31.9066147859922, Blast_Score=112, Evalue=3e-25, Organism=Drosophila melanogaster, GI28571526, Length=254, Percent_Identity=31.496062992126, Blast_Score=110, Evalue=6e-25, Organism=Drosophila melanogaster, GI21355319, Length=257, Percent_Identity=31.1284046692607, Blast_Score=109, Evalue=2e-24, Organism=Drosophila melanogaster, GI24644339, Length=254, Percent_Identity=30.7086614173228, Blast_Score=105, Evalue=2e-23, Organism=Drosophila melanogaster, GI17737361, Length=249, Percent_Identity=30.1204819277108, Blast_Score=91, Evalue=1e-18, Organism=Drosophila melanogaster, GI18859837, Length=195, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=2e-17, Organism=Drosophila melanogaster, GI24643142, Length=243, Percent_Identity=31.6872427983539, Blast_Score=86, Evalue=2e-17, Organism=Drosophila melanogaster, GI24644337, Length=251, Percent_Identity=30.2788844621514, Blast_Score=79, Evalue=3e-15, Organism=Drosophila melanogaster, GI21357673, Length=255, Percent_Identity=28.2352941176471, Blast_Score=79, Evalue=3e-15, Organism=Drosophila melanogaster, GI116007234, Length=250, Percent_Identity=30, Blast_Score=76, Evalue=2e-14, Organism=Drosophila melanogaster, GI116007236, Length=247, Percent_Identity=25.5060728744939, Blast_Score=71, Evalue=6e-13, Organism=Drosophila melanogaster, GI221331218, Length=209, Percent_Identity=29.6650717703349, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI24665243, Length=209, Percent_Identity=29.6650717703349, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI24580925, Length=201, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=7e-12, Organism=Drosophila melanogaster, GI24641232, Length=240, Percent_Identity=27.9166666666667, Blast_Score=67, Evalue=8e-12, Organism=Drosophila melanogaster, GI221331216, Length=210, Percent_Identity=29.5238095238095, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI24665247, Length=197, Percent_Identity=30.4568527918782, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI116007232, Length=196, Percent_Identity=30.6122448979592, Blast_Score=65, Evalue=4e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.159 [C]
Molecular weight: Translated: 26068; Mature: 25937
Theoretical pI: Translated: 4.81; Mature: 4.81
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 3.2 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGRFTDRTAVVTGAAQGIGAAYAAALAAEGADVVVADRNIDAGKTVAAEITASGGSAVFC CCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEECCCCEEEE EVDVADPDSATALAETTMQRFGRIDHLVNNAAIYGDMKLDLLITVPWDYYKKFMSVNMDG EEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECEEEEEEEECCHHHHHHHHHCCCCC ALNVTRAVYPHMQRSGGSIVNQSSTAAWVYSGFYGVAKAGLNSLTQQLAVELGGSNIRVN CCHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE AIAPGPIDTDATKTVTPGSIVADMVKRLPLKRMGTPQDLVGACLYLLSDEASWVTGQIFN EEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEE IDGGQIIRS CCCCCEECC >Mature Secondary Structure GRFTDRTAVVTGAAQGIGAAYAAALAAEGADVVVADRNIDAGKTVAAEITASGGSAVFC CCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEECCCCEEEE EVDVADPDSATALAETTMQRFGRIDHLVNNAAIYGDMKLDLLITVPWDYYKKFMSVNMDG EEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECEEEEEEEECCHHHHHHHHHCCCCC ALNVTRAVYPHMQRSGGSIVNQSSTAAWVYSGFYGVAKAGLNSLTQQLAVELGGSNIRVN CCHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE AIAPGPIDTDATKTVTPGSIVADMVKRLPLKRMGTPQDLVGACLYLLSDEASWVTGQIFN EEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEE IDGGQIIRS CCCCCEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]
Metal ions: CaCl2; KCl; MnCl2; NaCl [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]
Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]
Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]
General reaction: Redox reaction [C]
Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 8868443 [H]