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The map label for this gene is cobB2 [H]

Identifier: 54026431

GI number: 54026431

Start: 4626559

End: 4627305

Strand: Reverse

Name: cobB2 [H]

Synonym: nfa44580

Alternate gene names: 54026431

Gene position: 4627305-4626559 (Counterclockwise)

Preceding gene: 54026435

Following gene: 54026428

Centisome position: 76.85

GC content: 71.08

Gene sequence:

>747_bases
ATGACCAGGCAATGGCGCACCCGCTCGGGCCGGATCGGTGTCCTCACCGGCGCGGGCATCTCCACCGACTCCGGCATCCC
GGATTTCCGCGGCCCCCGCGGGGTGTGGACCGAGGACCCCATCGCCGAACTGATGTCGACCTACGACCAGTACCTGTCCG
ACCCGGACCTGCGCAGGCGCTCCTGGCTGGCCCGCCGCGCCAACCCCGCCTGGCAGGCCGAACCGAACGCCGGGCACCTC
GCCCTGGTCGACCTCGAGCGGGCGGGCCGGGCGGTCACGATCATCACCCAGAACGTCGACCGGCTGCACCAGCGCGCCGG
CTCCTCGCCCCAGCGGGTCGTCGAGATCCACGGCAACATGTTCGAGGTCGTCTGCGTCGGCTGCGACTACGAGACCGGCA
TGGCCGATGTGCTCGCCCGCGTCGAGGCGGGCGAGCCCGATCCGGCCTGCCCGGAGTGCGGCGGCATCCTCAAGGCCGCC
ACCATCATGTTCGGCCAGCAACTCGATCAGCGCACCATGACCAAGGCCGCGCTGACCGCCCAGACCAGTGACATCTTCCT
GGCGGTCGGCACCTCGTTGCAGGTGGAGCCGGCCGCCTCGATGTGCGCGCTCGCCGTCGACGCGGGCGCCGACCTGGTCA
TCGTCAACGCCGAACCCACCCCCTACGACAGCATCGCCACCGAGGTCGTCCACGAGCCGATCGGCACCGCCCTGCCCCGG
CTGGTGAAGGAGATCCTCGACGCCTGA

Upstream 100 bases:

>100_bases
TATCAGGGCGATGAGATAATTAGCATAGCAATCTCTCATTCTCTTGGCTTCCCCTGACGTGTCCGCCCACACCCCGGCCA
CGCCCACCTAGGCTGGCGGC

Downstream 100 bases:

>100_bases
ACGGTGCTCGCCGGGTGCCCGGTCAGGACGGGATCGGTCCGAGCACGCGCGTCAGATACTCGTTCGCGAACAGCCCCTCG
GGGTCGAACCGCTTGCGCAC

Product: Sir2 family regulator

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog 2 [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTRQWRTRSGRIGVLTGAGISTDSGIPDFRGPRGVWTEDPIAELMSTYDQYLSDPDLRRRSWLARRANPAWQAEPNAGHL
ALVDLERAGRAVTIITQNVDRLHQRAGSSPQRVVEIHGNMFEVVCVGCDYETGMADVLARVEAGEPDPACPECGGILKAA
TIMFGQQLDQRTMTKAALTAQTSDIFLAVGTSLQVEPAASMCALAVDAGADLVIVNAEPTPYDSIATEVVHEPIGTALPR
LVKEILDA

Sequences:

>Translated_248_residues
MTRQWRTRSGRIGVLTGAGISTDSGIPDFRGPRGVWTEDPIAELMSTYDQYLSDPDLRRRSWLARRANPAWQAEPNAGHL
ALVDLERAGRAVTIITQNVDRLHQRAGSSPQRVVEIHGNMFEVVCVGCDYETGMADVLARVEAGEPDPACPECGGILKAA
TIMFGQQLDQRTMTKAALTAQTSDIFLAVGTSLQVEPAASMCALAVDAGADLVIVNAEPTPYDSIATEVVHEPIGTALPR
LVKEILDA
>Mature_247_residues
TRQWRTRSGRIGVLTGAGISTDSGIPDFRGPRGVWTEDPIAELMSTYDQYLSDPDLRRRSWLARRANPAWQAEPNAGHLA
LVDLERAGRAVTIITQNVDRLHQRAGSSPQRVVEIHGNMFEVVCVGCDYETGMADVLARVEAGEPDPACPECGGILKAAT
IMFGQQLDQRTMTKAALTAQTSDIFLAVGTSLQVEPAASMCALAVDAGADLVIVNAEPTPYDSIATEVVHEPIGTALPRL
VKEILDA

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912664, Length=259, Percent_Identity=31.6602316602317, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI300795542, Length=248, Percent_Identity=31.4516129032258, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI300797577, Length=246, Percent_Identity=31.7073170731707, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI13787215, Length=241, Percent_Identity=31.9502074688797, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI6912662, Length=267, Percent_Identity=29.9625468164794, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI300797597, Length=241, Percent_Identity=31.5352697095436, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI6912660, Length=212, Percent_Identity=33.0188679245283, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI63054862, Length=208, Percent_Identity=32.6923076923077, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI13775602, Length=215, Percent_Identity=30.2325581395349, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI7657575, Length=236, Percent_Identity=27.5423728813559, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI13775600, Length=215, Percent_Identity=30.2325581395349, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI7706712, Length=244, Percent_Identity=30.327868852459, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI300797705, Length=195, Percent_Identity=29.7435897435897, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI215982798, Length=195, Percent_Identity=25.1282051282051, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI308199517, Length=240, Percent_Identity=33.75, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71990482, Length=264, Percent_Identity=30.6818181818182, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17567771, Length=265, Percent_Identity=28.6792452830189, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71990487, Length=266, Percent_Identity=30.4511278195489, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17505510, Length=237, Percent_Identity=27.8481012658228, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17541892, Length=241, Percent_Identity=29.4605809128631, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324504, Length=235, Percent_Identity=25.9574468085106, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320163, Length=256, Percent_Identity=25, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325242, Length=214, Percent_Identity=28.0373831775701, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24645650, Length=246, Percent_Identity=28.8617886178862, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28571445, Length=261, Percent_Identity=31.0344827586207, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648389, Length=206, Percent_Identity=30.0970873786408, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137536, Length=242, Percent_Identity=28.5123966942149, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24650933, Length=224, Percent_Identity=29.0178571428571, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571443, Length=221, Percent_Identity=28.9592760180996, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571441, Length=221, Percent_Identity=28.9592760180996, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26709; Mature: 26577

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQWRTRSGRIGVLTGAGISTDSGIPDFRGPRGVWTEDPIAELMSTYDQYLSDPDLRRR
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
SWLARRANPAWQAEPNAGHLALVDLERAGRAVTIITQNVDRLHQRAGSSPQRVVEIHGNM
HHHHHHCCCCCCCCCCCCCEEEEEHHHCCCEEEEEECCHHHHHHHHCCCHHHEEEECCCE
FEVVCVGCDYETGMADVLARVEAGEPDPACPECGGILKAATIMFGQQLDQRTMTKAALTA
EEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTSDIFLAVGTSLQVEPAASMCALAVDAGADLVIVNAEPTPYDSIATEVVHEPIGTALPR
CCCCEEEEECCCEEECCHHHHHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
LVKEILDA
HHHHHHCC
>Mature Secondary Structure 
TRQWRTRSGRIGVLTGAGISTDSGIPDFRGPRGVWTEDPIAELMSTYDQYLSDPDLRRR
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
SWLARRANPAWQAEPNAGHLALVDLERAGRAVTIITQNVDRLHQRAGSSPQRVVEIHGNM
HHHHHHCCCCCCCCCCCCCEEEEEHHHCCCEEEEEECCHHHHHHHHCCCHHHEEEECCCE
FEVVCVGCDYETGMADVLARVEAGEPDPACPECGGILKAATIMFGQQLDQRTMTKAALTA
EEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTSDIFLAVGTSLQVEPAASMCALAVDAGADLVIVNAEPTPYDSIATEVVHEPIGTALPR
CCCCEEEEECCCEEECCHHHHHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
LVKEILDA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]