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The map label for this gene is proP [H]

Identifier: 54026297

GI number: 54026297

Start: 4490133

End: 4491458

Strand: Reverse

Name: proP [H]

Synonym: nfa43260

Alternate gene names: 54026297

Gene position: 4491458-4490133 (Counterclockwise)

Preceding gene: 54026302

Following gene: 54026296

Centisome position: 74.59

GC content: 70.29

Gene sequence:

>1326_bases
ATGACCGAGACGCAGAATCCGGGACAATTGCGACGGTCGGTGGCCGCGTCGGCGATGGGCAACGCCACCGAGTGGTTCGA
CTACGGCGTCTACGCCGCGACTGCCACCTACCTCACCGACGCGTTCTTCCCCGGTGAACTCGGCACGCTGGGCACCATGC
TCGGCTTCGCCATCTCGTTCGTGCTGCGCCCCCTCGGCGGCATGGTCTGGGGCCCGCTGGGTGACCGGCTCGGCCGCAAG
CGGGTGCTGGCCAGCACGATCCTGTTGATGTCGGCGGCCACCGGCGCGATCGGCCTGCTGCCCACGCACGCCTCGGTCGG
CGTGCTCGCGCCCATCCTGCTGATCCTGCTGCGCGTGCTGCAGGGCTTCTCCACCGGCGGCGAGTACGGCGGCGCGGCCA
CCTACCTCGCCGAATCCGCCAGCGACCGCCGCCGCGGCTTCTTCGGCAGTTTCCTCGAGTTCGGCACGCTCGCCGGATTC
GTCGGCGGCTCGGCCGTGGTACTGGCCTGCCAGCTGGTGCTCGGCTCGGACGCCATGCACGACTGGGGCTGGCGCATCCC
GTTCCTGATCGCGGTGCCGCTCGGCCTGACCGGCCTGTACCTGCGCTCGCGGTTGGACGAGTCGCCGGTGTTCAAGGAGG
TGGCCCGGAACCCGCACCCGCCGACCGGCCTGCGCGAGCTGATCCTGCACTACCGCCGCGAGCTGCTCACCCTGGGCGGC
CTGGTGATCGCGCTGAACGTGGTGAACTACACGCTGCTGACCTACCAGCCGACCTACCTGCAGAAGACCATCGGCATGTC
CGAGTCCGCCACCACCACGATGATGCTCATCGGGCAGGTGGTGATCATGCTGGTGTTGCCGTTCTTCGGCGCGATCTCCG
ACCGGGTCGGCCGTCGCCCGATGTGGTTGTTCTCGCTGGTCGGCCTGGCCGTGTGCGCGGTGCCGATGTACTGGCTGATG
GGCCAGGGCCCGGTGTGGGCGGTGCTCGGCTTCGTGGTGCTCGGCCTGCTGTACGTGCCGCAGCTGTCCACCATCAGCGC
CACCTTCCCGGCGATCTTCCCCACCAATGTCCGGTACGCGGGCTTCGCGCTGGCCTACAACGTCTCGACCGCGGCCTTCG
GCGGCACCGCGCCGCTGGTGAACGAGGCAGTCATCGAGTCGACCGGCTGGGCGCTGTTCCCCGCCCTCTACATGGTGGGC
GCCTCGCTGCTCGGCCTGGTCGCCTGGGCCTATCTGCGCGAGACCGCGGGCACGTCGCTGCGCGGTACCGAGGTTCCCGA
CGCCCAGCGGACGGCCGTTCCGGTCGCCGCCGCGGCGGCGGGCTGA

Upstream 100 bases:

>100_bases
GTGGGTAACGGTTCTTTTCGTGCCCGGACCGAACGTAGCGGGACGGGTGCGCGAGAGGCAGAACCGGAGGTCTGCGGACG
CGGGCCGGTGAGGAGGCGCA

Downstream 100 bases:

>100_bases
CCGGCGGCGCCCACGGTCACGGTCCGGCGTCGGGGGTCGGGCCGGGTTCGGGGGTGCGGTCGCCGGGGCGTCGCCGTCGA
GGGTGCGGGTGGGTCGCTCA

Product: putative transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTETQNPGQLRRSVAASAMGNATEWFDYGVYAATATYLTDAFFPGELGTLGTMLGFAISFVLRPLGGMVWGPLGDRLGRK
RVLASTILLMSAATGAIGLLPTHASVGVLAPILLILLRVLQGFSTGGEYGGAATYLAESASDRRRGFFGSFLEFGTLAGF
VGGSAVVLACQLVLGSDAMHDWGWRIPFLIAVPLGLTGLYLRSRLDESPVFKEVARNPHPPTGLRELILHYRRELLTLGG
LVIALNVVNYTLLTYQPTYLQKTIGMSESATTTMMLIGQVVIMLVLPFFGAISDRVGRRPMWLFSLVGLAVCAVPMYWLM
GQGPVWAVLGFVVLGLLYVPQLSTISATFPAIFPTNVRYAGFALAYNVSTAAFGGTAPLVNEAVIESTGWALFPALYMVG
ASLLGLVAWAYLRETAGTSLRGTEVPDAQRTAVPVAAAAAG

Sequences:

>Translated_441_residues
MTETQNPGQLRRSVAASAMGNATEWFDYGVYAATATYLTDAFFPGELGTLGTMLGFAISFVLRPLGGMVWGPLGDRLGRK
RVLASTILLMSAATGAIGLLPTHASVGVLAPILLILLRVLQGFSTGGEYGGAATYLAESASDRRRGFFGSFLEFGTLAGF
VGGSAVVLACQLVLGSDAMHDWGWRIPFLIAVPLGLTGLYLRSRLDESPVFKEVARNPHPPTGLRELILHYRRELLTLGG
LVIALNVVNYTLLTYQPTYLQKTIGMSESATTTMMLIGQVVIMLVLPFFGAISDRVGRRPMWLFSLVGLAVCAVPMYWLM
GQGPVWAVLGFVVLGLLYVPQLSTISATFPAIFPTNVRYAGFALAYNVSTAAFGGTAPLVNEAVIESTGWALFPALYMVG
ASLLGLVAWAYLRETAGTSLRGTEVPDAQRTAVPVAAAAAG
>Mature_440_residues
TETQNPGQLRRSVAASAMGNATEWFDYGVYAATATYLTDAFFPGELGTLGTMLGFAISFVLRPLGGMVWGPLGDRLGRKR
VLASTILLMSAATGAIGLLPTHASVGVLAPILLILLRVLQGFSTGGEYGGAATYLAESASDRRRGFFGSFLEFGTLAGFV
GGSAVVLACQLVLGSDAMHDWGWRIPFLIAVPLGLTGLYLRSRLDESPVFKEVARNPHPPTGLRELILHYRRELLTLGGL
VIALNVVNYTLLTYQPTYLQKTIGMSESATTTMMLIGQVVIMLVLPFFGAISDRVGRRPMWLFSLVGLAVCAVPMYWLMG
QGPVWAVLGFVVLGLLYVPQLSTISATFPAIFPTNVRYAGFALAYNVSTAAFGGTAPLVNEAVIESTGWALFPALYMVGA
SLLGLVAWAYLRETAGTSLRGTEVPDAQRTAVPVAAAAAG

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=451, Percent_Identity=39.0243902439024, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1788942, Length=373, Percent_Identity=35.1206434316354, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1788292, Length=387, Percent_Identity=30.4909560723514, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1789941, Length=430, Percent_Identity=31.8604651162791, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI87082231, Length=240, Percent_Identity=29.5833333333333, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082404, Length=393, Percent_Identity=22.9007633587786, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46888; Mature: 46757

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETQNPGQLRRSVAASAMGNATEWFDYGVYAATATYLTDAFFPGELGTLGTMLGFAISF
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VLRPLGGMVWGPLGDRLGRKRVLASTILLMSAATGAIGLLPTHASVGVLAPILLILLRVL
HHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHH
QGFSTGGEYGGAATYLAESASDRRRGFFGSFLEFGTLAGFVGGSAVVLACQLVLGSDAMH
HCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
DWGWRIPFLIAVPLGLTGLYLRSRLDESPVFKEVARNPHPPTGLRELILHYRRELLTLGG
HCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LVIALNVVNYTLLTYQPTYLQKTIGMSESATTTMMLIGQVVIMLVLPFFGAISDRVGRRP
HHHHHHHHHHHHEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
MWLFSLVGLAVCAVPMYWLMGQGPVWAVLGFVVLGLLYVPQLSTISATFPAIFPTNVRYA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHH
GFALAYNVSTAAFGGTAPLVNEAVIESTGWALFPALYMVGASLLGLVAWAYLRETAGTSL
CEEEEEECCHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RGTEVPDAQRTAVPVAAAAAG
CCCCCCCCHHHCCCHHHHCCC
>Mature Secondary Structure 
TETQNPGQLRRSVAASAMGNATEWFDYGVYAATATYLTDAFFPGELGTLGTMLGFAISF
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VLRPLGGMVWGPLGDRLGRKRVLASTILLMSAATGAIGLLPTHASVGVLAPILLILLRVL
HHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHH
QGFSTGGEYGGAATYLAESASDRRRGFFGSFLEFGTLAGFVGGSAVVLACQLVLGSDAMH
HCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
DWGWRIPFLIAVPLGLTGLYLRSRLDESPVFKEVARNPHPPTGLRELILHYRRELLTLGG
HCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LVIALNVVNYTLLTYQPTYLQKTIGMSESATTTMMLIGQVVIMLVLPFFGAISDRVGRRP
HHHHHHHHHHHHEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
MWLFSLVGLAVCAVPMYWLMGQGPVWAVLGFVVLGLLYVPQLSTISATFPAIFPTNVRYA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHH
GFALAYNVSTAAFGGTAPLVNEAVIESTGWALFPALYMVGASLLGLVAWAYLRETAGTSL
CEEEEEECCHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RGTEVPDAQRTAVPVAAAAAG
CCCCCCCCHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]