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The map label for this gene is gatA

Identifier: 54026229

GI number: 54026229

Start: 4411630

End: 4413102

Strand: Reverse

Name: gatA

Synonym: nfa42580

Alternate gene names: 54026229

Gene position: 4413102-4411630 (Counterclockwise)

Preceding gene: 54026230

Following gene: 54026225

Centisome position: 73.29

GC content: 71.62

Gene sequence:

>1473_bases
ATGAGCGACCTGACCACCCGCACCGCGGCCGAACTCGCGCAGCAGATCCACGCGCGCGAGATCAGCTCCGTCGAGGTCAC
CCAGGCCCACCTGGACCGCATCGCCGAGGTGGACGGCGAGCTGAACGCCTTCCTGCACGTGGCGGGCGAGCGCGCGCTGG
AGGCCGCCGCCGCGGTCGACGCCGCCCTGGCCGCGGGCGAGGCGCCCGCCTCCCCGCTGGCCGGTGTTCCCTTGGCGCTC
AAGGACGTTTTCACCACCACCGACATGCCGACCACCTGCGCGTCGAAGATCCTCGAGGGCTGGATGTCGCCCTACGACGC
GACGGTGACCACCAAGCTGCGCGCGGCGGGCATCCCGATCCTCGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCCT
CCACCGAGAACTCCGCCTACGGCCCGACCCGCAACCCGTGGGACACCACCCGCATTCCCGGCGGCTCCGGCGGCGGCTCG
GCGGCCGCGCTGGCCTCGCGTCAGGCGCCGCTGGCCATCGGTACCGATACCGGTGGCTCCATCCGTCAGCCCGCCGCCGT
CACCGCGACCGTCGGCACCAAGCCGACCTACGGCACCGTCTCCCGCTACGGGCTGGTCGCCTGCGCGTCCTCGCTCGACC
AGGGCGGGCCGTGTGGCCGCACGGTGCTCGACACCGCGTTGCTGCACGAGGTGATCGCGGGCTACGACCCGCGCGATTCC
ACCTCCCGGGACGTGCCGGTCCCGCCGGTGGTCGAGGCCGCTCGCCGGGGCGCCGCGGGCGACCTGCGCGGCGTCAAGGT
CGGCGTGGTCAAGGAGTTGCACTCCGACAGCTACCAGCCGGGCGTACTCGCCTCCTTCGACGCCGCGGTGGCGGTGCTGA
AGGATCTGGGCGCCGAGGTGGTCGAGGTGTCCTGCCCGCACTTCGAGTACGGCCTGCCGTCCTACTACCTGGTGATGCCG
TCGGAGGTGTCGTCGAACCTGGCCCGCTTCGACGCCATGCGGTACGGCCTGCGCGTCGGCGACGACGGCACGCACAGCGC
CGAGCAGGTGATGGCGATGACCAGGGCCGCCGGTTTCGGCCCGGAGGTCAAGCGCCGCATCATGATCGGCACCTACGCCC
TGTCGGCCGGCTATTACGACGAGTACTACGGCCAGGCGCTCAAGGTGCGCACCCTCATCGCCCGCGATTTCGACCGTGCC
TACGAGCAGGTCGACGTGCTGGTCTCGCCGACCAGTCCGTTCACCCCGTGGAAGCTGGGCGAGAAGGTCGACGATCCGCT
GGCGATGTACCTGTCGGATCTGTGCACCCTGCCCACCAACCTGGCCGGGCACCCGGCCATGTCGGTGCCGTCCGGGCTGA
GCAAGGACGACGGCATGCCGGTGGGCCTGCAGATCATGGCGCCCGCGCTGGCCGACGACCGGCTCTACCGGGTGGGCGCG
GCGTACGAGGCCGCGCGCGGTCCGATCGCCTGA

Upstream 100 bases:

>100_bases
ACACCGTGGTGCCCGGCCTGACGCCGGACCAGGCGCTGGCCGCCGCGCCGGCGGTCGAGGAGCAGCGGTTCATGGTTCCG
CAGATTCTGGGAGAGGGCGA

Downstream 100 bases:

>100_bases
AGCAGGGTGTGAGCGCCGCTGAGGACGCCCGCCGTCCTCAGCGGGCGCGCAGGGTTTCGGCCTGCCGCTGGACGGCGGCG
GTCATCCCCTCGAAGCCGTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSDLTTRTAAELAQQIHAREISSVEVTQAHLDRIAEVDGELNAFLHVAGERALEAAAAVDAALAAGEAPASPLAGVPLAL
KDVFTTTDMPTTCASKILEGWMSPYDATVTTKLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWDTTRIPGGSGGGS
AAALASRQAPLAIGTDTGGSIRQPAAVTATVGTKPTYGTVSRYGLVACASSLDQGGPCGRTVLDTALLHEVIAGYDPRDS
TSRDVPVPPVVEAARRGAAGDLRGVKVGVVKELHSDSYQPGVLASFDAAVAVLKDLGAEVVEVSCPHFEYGLPSYYLVMP
SEVSSNLARFDAMRYGLRVGDDGTHSAEQVMAMTRAAGFGPEVKRRIMIGTYALSAGYYDEYYGQALKVRTLIARDFDRA
YEQVDVLVSPTSPFTPWKLGEKVDDPLAMYLSDLCTLPTNLAGHPAMSVPSGLSKDDGMPVGLQIMAPALADDRLYRVGA
AYEAARGPIA

Sequences:

>Translated_490_residues
MSDLTTRTAAELAQQIHAREISSVEVTQAHLDRIAEVDGELNAFLHVAGERALEAAAAVDAALAAGEAPASPLAGVPLAL
KDVFTTTDMPTTCASKILEGWMSPYDATVTTKLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWDTTRIPGGSGGGS
AAALASRQAPLAIGTDTGGSIRQPAAVTATVGTKPTYGTVSRYGLVACASSLDQGGPCGRTVLDTALLHEVIAGYDPRDS
TSRDVPVPPVVEAARRGAAGDLRGVKVGVVKELHSDSYQPGVLASFDAAVAVLKDLGAEVVEVSCPHFEYGLPSYYLVMP
SEVSSNLARFDAMRYGLRVGDDGTHSAEQVMAMTRAAGFGPEVKRRIMIGTYALSAGYYDEYYGQALKVRTLIARDFDRA
YEQVDVLVSPTSPFTPWKLGEKVDDPLAMYLSDLCTLPTNLAGHPAMSVPSGLSKDDGMPVGLQIMAPALADDRLYRVGA
AYEAARGPIA
>Mature_489_residues
SDLTTRTAAELAQQIHAREISSVEVTQAHLDRIAEVDGELNAFLHVAGERALEAAAAVDAALAAGEAPASPLAGVPLALK
DVFTTTDMPTTCASKILEGWMSPYDATVTTKLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWDTTRIPGGSGGGSA
AALASRQAPLAIGTDTGGSIRQPAAVTATVGTKPTYGTVSRYGLVACASSLDQGGPCGRTVLDTALLHEVIAGYDPRDST
SRDVPVPPVVEAARRGAAGDLRGVKVGVVKELHSDSYQPGVLASFDAAVAVLKDLGAEVVEVSCPHFEYGLPSYYLVMPS
EVSSNLARFDAMRYGLRVGDDGTHSAEQVMAMTRAAGFGPEVKRRIMIGTYALSAGYYDEYYGQALKVRTLIARDFDRAY
EQVDVLVSPTSPFTPWKLGEKVDDPLAMYLSDLCTLPTNLAGHPAMSVPSGLSKDDGMPVGLQIMAPALADDRLYRVGAA
YEAARGPIA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=37.4, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI195972892, Length=499, Percent_Identity=25.0501002004008, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI166795287, Length=196, Percent_Identity=31.1224489795918, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=418, Percent_Identity=36.6028708133971, Blast_Score=251, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI17537465, Length=497, Percent_Identity=25.1509054325956, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=317, Percent_Identity=28.7066246056782, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=304, Percent_Identity=27.3026315789474, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=188, Percent_Identity=27.6595744680851, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=414, Percent_Identity=35.9903381642512, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6319685, Length=418, Percent_Identity=29.9043062200957, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=201, Percent_Identity=28.8557213930348, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648113, Length=465, Percent_Identity=40.6451612903226, Blast_Score=291, Evalue=9e-79,
Organism=Drosophila melanogaster, GI24652985, Length=489, Percent_Identity=25.3578732106339, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI19922090, Length=489, Percent_Identity=25.3578732106339, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24652981, Length=489, Percent_Identity=25.3578732106339, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24652983, Length=489, Percent_Identity=25.3578732106339, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24644968, Length=517, Percent_Identity=26.6924564796905, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI45550774, Length=209, Percent_Identity=34.4497607655502, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648435, Length=209, Percent_Identity=34.4497607655502, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648437, Length=209, Percent_Identity=34.4497607655502, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356731, Length=236, Percent_Identity=31.3559322033898, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI161078093, Length=526, Percent_Identity=26.425855513308, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648441, Length=134, Percent_Identity=35.8208955223881, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648439, Length=134, Percent_Identity=35.8208955223881, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_NOCFA (Q5YRT4)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120471.1
- ProteinModelPortal:   Q5YRT4
- SMR:   Q5YRT4
- GeneID:   3109639
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa42580
- NMPDR:   fig|247156.1.peg.4105
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   NFAR247156:NFA42580-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: NA

Molecular weight: Translated: 51388; Mature: 51256

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 81-81 ACT_SITE 156-156 ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLTTRTAAELAQQIHAREISSVEVTQAHLDRIAEVDGELNAFLHVAGERALEAAAAVD
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AALAAGEAPASPLAGVPLALKDVFTTTDMPTTCASKILEGWMSPYDATVTTKLRAAGIPI
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHEEEHHHCCCCE
LGKTNMDEFAMGSSTENSAYGPTRNPWDTTRIPGGSGGGSAAALASRQAPLAIGTDTGGS
ECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCC
IRQPAAVTATVGTKPTYGTVSRYGLVACASSLDQGGPCGRTVLDTALLHEVIAGYDPRDS
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
TSRDVPVPPVVEAARRGAAGDLRGVKVGVVKELHSDSYQPGVLASFDAAVAVLKDLGAEV
CCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCE
VEVSCPHFEYGLPSYYLVMPSEVSSNLARFDAMRYGLRVGDDGTHSAEQVMAMTRAAGFG
EEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHCCCC
PEVKRRIMIGTYALSAGYYDEYYGQALKVRTLIARDFDRAYEQVDVLVSPTSPFTPWKLG
HHHHHEEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCC
EKVDDPLAMYLSDLCTLPTNLAGHPAMSVPSGLSKDDGMPVGLQIMAPALADDRLYRVGA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHCCCHHEECCC
AYEAARGPIA
HHHHCCCCCC
>Mature Secondary Structure 
SDLTTRTAAELAQQIHAREISSVEVTQAHLDRIAEVDGELNAFLHVAGERALEAAAAVD
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AALAAGEAPASPLAGVPLALKDVFTTTDMPTTCASKILEGWMSPYDATVTTKLRAAGIPI
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHEEEHHHCCCCE
LGKTNMDEFAMGSSTENSAYGPTRNPWDTTRIPGGSGGGSAAALASRQAPLAIGTDTGGS
ECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCC
IRQPAAVTATVGTKPTYGTVSRYGLVACASSLDQGGPCGRTVLDTALLHEVIAGYDPRDS
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
TSRDVPVPPVVEAARRGAAGDLRGVKVGVVKELHSDSYQPGVLASFDAAVAVLKDLGAEV
CCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCE
VEVSCPHFEYGLPSYYLVMPSEVSSNLARFDAMRYGLRVGDDGTHSAEQVMAMTRAAGFG
EEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHCCCC
PEVKRRIMIGTYALSAGYYDEYYGQALKVRTLIARDFDRAYEQVDVLVSPTSPFTPWKLG
HHHHHEEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCC
EKVDDPLAMYLSDLCTLPTNLAGHPAMSVPSGLSKDDGMPVGLQIMAPALADDRLYRVGA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHCCCHHEECCC
AYEAARGPIA
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA