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The map label for this gene is pfkA [H]

Identifier: 54026212

GI number: 54026212

Start: 4390165

End: 4391199

Strand: Reverse

Name: pfkA [H]

Synonym: nfa42410

Alternate gene names: 54026212

Gene position: 4391199-4390165 (Counterclockwise)

Preceding gene: 54026213

Following gene: 54026211

Centisome position: 72.93

GC content: 68.89

Gene sequence:

>1035_bases
ATGCGCATCGGAGTCTTGACCGGAGGCGGGGACTGTCCAGGACTGAACGCGGTCATCCGCGCTGTCGTTCGTACCGCGAA
CGGCCGCTACGGTGACGCGATCGTGGGATTCCAGGACGGCTGGCGCGGCCTATTGGAAGACCGCAAGATCAACATCCTCA
ACGACGACCGCACCGACCGGCTGCTCAACAAGGGCGGCACCATGCTCGGCACCGCGCGCACCAACCCCGACGTGCTGCGC
GCCGGGCTCGGCAAGATCAAACAGACGCTCGACGACAACGGCATCGAGGCACTCATCCCGATCGGCGGCGAGGGCACCCT
CACCGCGGCCAGCTGGCTCTCCGACGAGGGGGTGCCGGTGGTCGGCGTCCCCAAGACCATCGACAACGACATCGACTGCA
CCGATGTCACCTTCGGCCACGACACCGCCCTGAGCATCGCCACCGAGGCCATCGACCGGCTGCACACCACCGCCGAATCG
CACCAGCGCGTGATGCTGGTCGAGGTGATGGGCAGGCACGCGGGCTGGATCGCGGTCAACTCGGGCATGGCCGCGGGCGC
CCACCTCACCCTGGTGCCGGAGGAACCCTTCGACGTCGACGAGGTGTGCACGATGATCAAGCGGCGCTTCCAGCGCGGCG
ACAAGCATTTCATCTGCGTGGTGGCCGAGGGCTCGCATCCCGCGCCCGATTCCGGGTTCACCCTGCGCGAGGGTGGTGTC
GACGAGTTCGGCCACGAGCGGTTCACCGGCGTGGCGCAACAGCTGGGCACCGAGATCGAGCGCCGCATCGGCAAGGAGGT
GCGCACCACCGTCCTCGGTCACGTGCAGCGCGGCGGCAGCCCCACCCCCTACGACCGGGTACTGGCGACCCGCTTCGGCC
TGCACGCCGCCGAGGCCGTGCACGCCGGACATTTCGGCCAGATGGTCGCGCTGCACGGCACCGCCATCGAACTGGTGCCG
CTGGCCGAGGCGACCAAGCAGCTCAAGCGCGTGCCCACCGAGCGGTACCGGGAGGCGCAGGCGTTCTTCGGGTGA

Upstream 100 bases:

>100_bases
ACGAACTCGCGGGTGGCAGCTGCCCGTCCTGACCGGGCGCGACGCGGCGTTGCCGGGGTCGCGGCTGCCGGAATCGCGCG
CGAGGGGCAAGATAGCGGCC

Downstream 100 bases:

>100_bases
CCCGCTGACATCGTCGGGGCCGCGAATTAGGCTGGCCGCCATGGTTTTCCTCGACGACGCCCCGGCCTGCCCGAACAGCT
TGGATCTGCCCGCCGAGACG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRIGVLTGGGDCPGLNAVIRAVVRTANGRYGDAIVGFQDGWRGLLEDRKINILNDDRTDRLLNKGGTMLGTARTNPDVLR
AGLGKIKQTLDDNGIEALIPIGGEGTLTAASWLSDEGVPVVGVPKTIDNDIDCTDVTFGHDTALSIATEAIDRLHTTAES
HQRVMLVEVMGRHAGWIAVNSGMAAGAHLTLVPEEPFDVDEVCTMIKRRFQRGDKHFICVVAEGSHPAPDSGFTLREGGV
DEFGHERFTGVAQQLGTEIERRIGKEVRTTVLGHVQRGGSPTPYDRVLATRFGLHAAEAVHAGHFGQMVALHGTAIELVP
LAEATKQLKRVPTERYREAQAFFG

Sequences:

>Translated_344_residues
MRIGVLTGGGDCPGLNAVIRAVVRTANGRYGDAIVGFQDGWRGLLEDRKINILNDDRTDRLLNKGGTMLGTARTNPDVLR
AGLGKIKQTLDDNGIEALIPIGGEGTLTAASWLSDEGVPVVGVPKTIDNDIDCTDVTFGHDTALSIATEAIDRLHTTAES
HQRVMLVEVMGRHAGWIAVNSGMAAGAHLTLVPEEPFDVDEVCTMIKRRFQRGDKHFICVVAEGSHPAPDSGFTLREGGV
DEFGHERFTGVAQQLGTEIERRIGKEVRTTVLGHVQRGGSPTPYDRVLATRFGLHAAEAVHAGHFGQMVALHGTAIELVP
LAEATKQLKRVPTERYREAQAFFG
>Mature_344_residues
MRIGVLTGGGDCPGLNAVIRAVVRTANGRYGDAIVGFQDGWRGLLEDRKINILNDDRTDRLLNKGGTMLGTARTNPDVLR
AGLGKIKQTLDDNGIEALIPIGGEGTLTAASWLSDEGVPVVGVPKTIDNDIDCTDVTFGHDTALSIATEAIDRLHTTAES
HQRVMLVEVMGRHAGWIAVNSGMAAGAHLTLVPEEPFDVDEVCTMIKRRFQRGDKHFICVVAEGSHPAPDSGFTLREGGV
DEFGHERFTGVAQQLGTEIERRIGKEVRTTVLGHVQRGGSPTPYDRVLATRFGLHAAEAVHAGHFGQMVALHGTAIELVP
LAEATKQLKRVPTERYREAQAFFG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=361, Percent_Identity=33.7950138504155, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI48762920, Length=377, Percent_Identity=33.42175066313, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI266453768, Length=360, Percent_Identity=34.4444444444444, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI266453748, Length=360, Percent_Identity=34.4444444444444, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4505749, Length=360, Percent_Identity=34.4444444444444, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI266453619, Length=360, Percent_Identity=34.4444444444444, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI1790350, Length=330, Percent_Identity=40.6060606060606, Blast_Score=226, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI25147584, Length=369, Percent_Identity=29.2682926829268, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17558788, Length=327, Percent_Identity=32.4159021406728, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI72003601, Length=366, Percent_Identity=25.9562841530055, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6323861, Length=364, Percent_Identity=34.0659340659341, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321679, Length=365, Percent_Identity=33.6986301369863, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI28573326, Length=359, Percent_Identity=34.5403899721448, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24652337, Length=359, Percent_Identity=34.2618384401114, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI17647809, Length=359, Percent_Identity=34.2618384401114, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 37052; Mature: 37052

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGVLTGGGDCPGLNAVIRAVVRTANGRYGDAIVGFQDGWRGLLEDRKINILNDDRTDR
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCCEEEEECCCHHHH
LLNKGGTMLGTARTNPDVLRAGLGKIKQTLDDNGIEALIPIGGEGTLTAASWLSDEGVPV
HHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHCCCCCCEE
VGVPKTIDNDIDCTDVTFGHDTALSIATEAIDRLHTTAESHQRVMLVEVMGRHAGWIAVN
EECCHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEHHCCCCCEEEEE
SGMAAGAHLTLVPEEPFDVDEVCTMIKRRFQRGDKHFICVVAEGSHPAPDSGFTLREGGV
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEECCCCC
DEFGHERFTGVAQQLGTEIERRIGKEVRTTVLGHVQRGGSPTPYDRVLATRFGLHAAEAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHH
HAGHFGQMVALHGTAIELVPLAEATKQLKRVPTERYREAQAFFG
HHCCCCCEEEECCCEEEEEEHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MRIGVLTGGGDCPGLNAVIRAVVRTANGRYGDAIVGFQDGWRGLLEDRKINILNDDRTDR
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCCEEEEECCCHHHH
LLNKGGTMLGTARTNPDVLRAGLGKIKQTLDDNGIEALIPIGGEGTLTAASWLSDEGVPV
HHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHCCCCCCEE
VGVPKTIDNDIDCTDVTFGHDTALSIATEAIDRLHTTAESHQRVMLVEVMGRHAGWIAVN
EECCHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEHHCCCCCEEEEE
SGMAAGAHLTLVPEEPFDVDEVCTMIKRRFQRGDKHFICVVAEGSHPAPDSGFTLREGGV
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEECCCCC
DEFGHERFTGVAQQLGTEIERRIGKEVRTTVLGHVQRGGSPTPYDRVLATRFGLHAAEAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHH
HAGHFGQMVALHGTAIELVPLAEATKQLKRVPTERYREAQAFFG
HHCCCCCEEEECCCEEEEEEHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]