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The map label for this gene is gatB

Identifier: 54026209

GI number: 54026209

Start: 4386629

End: 4388140

Strand: Reverse

Name: gatB

Synonym: nfa42380

Alternate gene names: 54026209

Gene position: 4388140-4386629 (Counterclockwise)

Preceding gene: 54026210

Following gene: 54026206

Centisome position: 72.88

GC content: 69.58

Gene sequence:

>1512_bases
ATGGCCATGAGCGCACCCACGGTCGAGCTGATGGATTACGCCGATGTCGTCGCCCGGTACGAGCCGGTGCTCGGCATGGA
GGTCCACGTCGAACTCTCCACCGCGACCAAGATGTTCTGCGGTTGCCCGACCGATTTCGGCGCCGAGCCGAACACCCAGG
TGTGCCCGGTGTGCCTCGGCCTGCCCGGCTCGCTGCCGGTCGTCAACGAGAAGGCCGTCGAATCGGCCATCCGCATCGGC
CTGGCGCTGAACTGTTCGATCACCCCGTGGGGCCGGTTCGCGCGCAAGAACTACTTCTACCCGGACCAGCCGAAGAACTA
CCAGATCAGCCAGTACGACGAGCCGATCGCCACCGACGGTCACCTCGATGTGGTGCTCGACGACGGCAGCGTGTTCCGGG
TCGACATCGAGCGCGCGCACATGGAGGAGGACACCGGCAAGTCCGTGCACGTGGGCGGCGCCACCGGCCGCATCCACGGC
GCCAGCCACTCGCTGCTGGACTACAACCGTGCCGGGGTGCCGCTGATCGAGATCGTCACCAAGCCGATCACCGGGGCGGG
GGAGCGGGCGCCCGAGGTGGCCCGCGCCTACGTCACCGCGCTGCGCGATCTGCTCAAGTCGCTGGGCGTGTCGGATGTGA
AGATGGAGCAGGGCTCGCTGCGCTGCGACGCCAACGTCTCGCTGATGCCGGTCGGCGCGAGCGAGTTCGGCACCCGCACC
GAGACCAAGAACGTCAACTCCCTCAAGAGCGTCGAGGTCGCCGTGCGCTACGAGATGCGCCGCCAGGCCGCCGTACTGGC
CGCGGGCGGCGCCATCGTGCAGGAGACCAGGCACTTCCACGAGTCCGACGGCACCACCTCGCCCGGCCGCCGCAAGGAGA
CCGCCGAGGACTACCGCTACTTCCCCGAGCCCGACCTCGAGCCGATCGCCCCGTCCCCGGAGTGGATCGAGGAACTGCGC
GCGACCATTCCCGAGTACCCGTGGCTGCGTCGGGCCCGCATCCAGCAGGAGTGGGGGGTCTCCGACGAGGTCATGCGCGA
CGTCGTCAACGCGGGCGCGCTGGATCTCATCATCGCCACCGTCGAGGCGGGCGCACCGGCGAACGAGGCCCGCTCCTGGT
GGGTCGCCTACCTCAGCGAGAAGGCCAAGGAACGCGGCGTCGCGCTCGACGAGCTGCCCATCACCCCGGCCCAGGTCGCC
GAGGTGGTGAAGCTGGTCGAGTCCAAGACGGTCAACAGCAAGGTCGCCAAGCAGGTCGTCGACTTCGTGCTGGCGGGCGA
GGGGGAACCGGCCGCCGTCGTCGAGGCCAAGGGTCTGGGCATGGTCAGCGACGACAGCGCGCTGCAGGCCGAGGTGGAGA
AGGCGCTGGCCGCCAACCCCGACATCGCCGACAAGATCCGCGCCGGGAAGGTGCAGGCCGCGGGCAAGATCGTCGGTGAC
GTCATGAAGGCGACCCGCGGCCAGGCCGACGCCGCCCGGGTGCGCGAGCTCGTGCTCGCCGCCTGCTCCTGA

Upstream 100 bases:

>100_bases
GAAGTCCTCACCGCCGTCACCGAGTGGCTGACCGACCGGGTCCCGCCGCGGTGACCGCGAGGAGGTCGTGGCGATCGCGC
GGGCGGGCTTACCTAGACTG

Downstream 100 bases:

>100_bases
GCGGTGCCGCCCGCGGTGACACCGCCGCGGGCGGATCCGGCCGGGTGCGCTCGCTGCGCGATCAGTGCGCCGCGAGCGCG
TCCCGGTAGCGGTCGAGGCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MAMSAPTVELMDYADVVARYEPVLGMEVHVELSTATKMFCGCPTDFGAEPNTQVCPVCLGLPGSLPVVNEKAVESAIRIG
LALNCSITPWGRFARKNYFYPDQPKNYQISQYDEPIATDGHLDVVLDDGSVFRVDIERAHMEEDTGKSVHVGGATGRIHG
ASHSLLDYNRAGVPLIEIVTKPITGAGERAPEVARAYVTALRDLLKSLGVSDVKMEQGSLRCDANVSLMPVGASEFGTRT
ETKNVNSLKSVEVAVRYEMRRQAAVLAAGGAIVQETRHFHESDGTTSPGRRKETAEDYRYFPEPDLEPIAPSPEWIEELR
ATIPEYPWLRRARIQQEWGVSDEVMRDVVNAGALDLIIATVEAGAPANEARSWWVAYLSEKAKERGVALDELPITPAQVA
EVVKLVESKTVNSKVAKQVVDFVLAGEGEPAAVVEAKGLGMVSDDSALQAEVEKALAANPDIADKIRAGKVQAAGKIVGD
VMKATRGQADAARVRELVLAACS

Sequences:

>Translated_503_residues
MAMSAPTVELMDYADVVARYEPVLGMEVHVELSTATKMFCGCPTDFGAEPNTQVCPVCLGLPGSLPVVNEKAVESAIRIG
LALNCSITPWGRFARKNYFYPDQPKNYQISQYDEPIATDGHLDVVLDDGSVFRVDIERAHMEEDTGKSVHVGGATGRIHG
ASHSLLDYNRAGVPLIEIVTKPITGAGERAPEVARAYVTALRDLLKSLGVSDVKMEQGSLRCDANVSLMPVGASEFGTRT
ETKNVNSLKSVEVAVRYEMRRQAAVLAAGGAIVQETRHFHESDGTTSPGRRKETAEDYRYFPEPDLEPIAPSPEWIEELR
ATIPEYPWLRRARIQQEWGVSDEVMRDVVNAGALDLIIATVEAGAPANEARSWWVAYLSEKAKERGVALDELPITPAQVA
EVVKLVESKTVNSKVAKQVVDFVLAGEGEPAAVVEAKGLGMVSDDSALQAEVEKALAANPDIADKIRAGKVQAAGKIVGD
VMKATRGQADAARVRELVLAACS
>Mature_502_residues
AMSAPTVELMDYADVVARYEPVLGMEVHVELSTATKMFCGCPTDFGAEPNTQVCPVCLGLPGSLPVVNEKAVESAIRIGL
ALNCSITPWGRFARKNYFYPDQPKNYQISQYDEPIATDGHLDVVLDDGSVFRVDIERAHMEEDTGKSVHVGGATGRIHGA
SHSLLDYNRAGVPLIEIVTKPITGAGERAPEVARAYVTALRDLLKSLGVSDVKMEQGSLRCDANVSLMPVGASEFGTRTE
TKNVNSLKSVEVAVRYEMRRQAAVLAAGGAIVQETRHFHESDGTTSPGRRKETAEDYRYFPEPDLEPIAPSPEWIEELRA
TIPEYPWLRRARIQQEWGVSDEVMRDVVNAGALDLIIATVEAGAPANEARSWWVAYLSEKAKERGVALDELPITPAQVAE
VVKLVESKTVNSKVAKQVVDFVLAGEGEPAAVVEAKGLGMVSDDSALQAEVEKALAANPDIADKIRAGKVQAAGKIVGDV
MKATRGQADAARVRELVLAACS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=502, Percent_Identity=35.0597609561753, Blast_Score=285, Evalue=9e-77,
Organism=Caenorhabditis elegans, GI115534094, Length=308, Percent_Identity=39.2857142857143, Blast_Score=212, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=313, Percent_Identity=33.5463258785943, Blast_Score=170, Evalue=5e-43,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=30.3643724696356, Blast_Score=233, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_NOCFA (Q5YRV4)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120451.1
- ProteinModelPortal:   Q5YRV4
- SMR:   Q5YRV4
- GeneID:   3109640
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa42380
- NMPDR:   fig|247156.1.peg.4086
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   NFAR247156:NFA42380-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: NA

Molecular weight: Translated: 54306; Mature: 54175

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSAPTVELMDYADVVARYEPVLGMEVHVELSTATKMFCGCPTDFGAEPNTQVCPVCLG
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHCCCCCCCCCCCCCCCCEEEEC
LPGSLPVVNEKAVESAIRIGLALNCSITPWGRFARKNYFYPDQPKNYQISQYDEPIATDG
CCCCCCCCCHHHHHHHHHHCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
HLDVVLDDGSVFRVDIERAHMEEDTGKSVHVGGATGRIHGASHSLLDYNRAGVPLIEIVT
CEEEEECCCCEEEEEHHHHHCCCCCCCEEEECCCCCEEECCCCCHHCCCCCCCCHHHHHH
KPITGAGERAPEVARAYVTALRDLLKSLGVSDVKMEQGSLRCDANVSLMPVGASEFGTRT
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCEEEECCCHHHCCCC
ETKNVNSLKSVEVAVRYEMRRQAAVLAAGGAIVQETRHFHESDGTTSPGRRKETAEDYRY
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHCCC
FPEPDLEPIAPSPEWIEELRATIPEYPWLRRARIQQEWGVSDEVMRDVVNAGALDLIIAT
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEE
VEAGAPANEARSWWVAYLSEKAKERGVALDELPITPAQVAEVVKLVESKTVNSKVAKQVV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
DFVLAGEGEPAAVVEAKGLGMVSDDSALQAEVEKALAANPDIADKIRAGKVQAAGKIVGD
HHHHCCCCCCCEEEEECCCCEECCCHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHH
VMKATRGQADAARVRELVLAACS
HHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AMSAPTVELMDYADVVARYEPVLGMEVHVELSTATKMFCGCPTDFGAEPNTQVCPVCLG
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHCCCCCCCCCCCCCCCCEEEEC
LPGSLPVVNEKAVESAIRIGLALNCSITPWGRFARKNYFYPDQPKNYQISQYDEPIATDG
CCCCCCCCCHHHHHHHHHHCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
HLDVVLDDGSVFRVDIERAHMEEDTGKSVHVGGATGRIHGASHSLLDYNRAGVPLIEIVT
CEEEEECCCCEEEEEHHHHHCCCCCCCEEEECCCCCEEECCCCCHHCCCCCCCCHHHHHH
KPITGAGERAPEVARAYVTALRDLLKSLGVSDVKMEQGSLRCDANVSLMPVGASEFGTRT
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCEEEECCCHHHCCCC
ETKNVNSLKSVEVAVRYEMRRQAAVLAAGGAIVQETRHFHESDGTTSPGRRKETAEDYRY
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHCCC
FPEPDLEPIAPSPEWIEELRATIPEYPWLRRARIQQEWGVSDEVMRDVVNAGALDLIIAT
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEE
VEAGAPANEARSWWVAYLSEKAKERGVALDELPITPAQVAEVVKLVESKTVNSKVAKQVV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
DFVLAGEGEPAAVVEAKGLGMVSDDSALQAEVEKALAANPDIADKIRAGKVQAAGKIVGD
HHHHCCCCCCCEEEEECCCCEECCCHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHH
VMKATRGQADAARVRELVLAACS
HHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA