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The map label for this gene is mcrA [C]

Identifier: 54026197

GI number: 54026197

Start: 4375303

End: 4375674

Strand: Reverse

Name: mcrA [C]

Synonym: nfa42260

Alternate gene names: 54026197

Gene position: 4375674-4375303 (Counterclockwise)

Preceding gene: 54026200

Following gene: 54026196

Centisome position: 72.67

GC content: 62.1

Gene sequence:

>372_bases
GTGGAGATCCGGGAGGGGACCCTGCTGGCGGCGAATCATTATCGTCGTGAGCGGAATCCGAAGTTGCGGAAGGCGAAGCT
GGCGGAATTTCGCCGGGTGCATTCCCGGGTTTTCTGTGAGGTCTGTGGCTTCGACTTCGAAGCGGTGTACGGGGAGCGCG
GGGCGGGCTATATCGAATGCCATCACATTGTGCCGCTGCATGTTTCGGGGGAGACCGTGACGAAGCTGTCGGATCTGGTG
CTGCTGTGCTCGAACTGTCACCGGATGATTCACCACGGGTCGCGGTGGTTGAGTCCGGAAGAGTTGCGCGAGGTTGTTCA
GGTGTCCGGACAGCCGGCCGAACCCGGGGACGGCGAGCGGGTGGAGTTCTGA

Upstream 100 bases:

>100_bases
CGGCGGGCACGATGCCGAGACGATCCGTGCTCTGCTTGAGGACATTCCCGCCACGCCGCCGACCGAATCGCTCGGTTTCG
ACGTCGCCTTCATCGATTCG

Downstream 100 bases:

>100_bases
GGTTCCGATTCGATTACGAAAACCCTGGTGGGGCCCGGTCTGAGGGCGGGTGCGACCACTTCGGCGGCTGTTTTGACGGC
GCGTGCCCGCACTTGCGGGG

Product: putative endonuclease

Products: NA

Alternate protein names: Restriction Endonuclease-Like Protein; Endonuclease; HNH Endonuclease Family Protein; Restriction Endonuclease; Hnh Endonuclease; Endonuclease-Like Protein; 5-Methylcytosine-Specific Restriction A

Number of amino acids: Translated: 123; Mature: 123

Protein sequence:

>123_residues
MEIREGTLLAANHYRRERNPKLRKAKLAEFRRVHSRVFCEVCGFDFEAVYGERGAGYIECHHIVPLHVSGETVTKLSDLV
LLCSNCHRMIHHGSRWLSPEELREVVQVSGQPAEPGDGERVEF

Sequences:

>Translated_123_residues
MEIREGTLLAANHYRRERNPKLRKAKLAEFRRVHSRVFCEVCGFDFEAVYGERGAGYIECHHIVPLHVSGETVTKLSDLV
LLCSNCHRMIHHGSRWLSPEELREVVQVSGQPAEPGDGERVEF
>Mature_123_residues
MEIREGTLLAANHYRRERNPKLRKAKLAEFRRVHSRVFCEVCGFDFEAVYGERGAGYIECHHIVPLHVSGETVTKLSDLV
LLCSNCHRMIHHGSRWLSPEELREVVQVSGQPAEPGDGERVEF

Specific function: Restriction Of 5-Methyl And 5-Hydroxymethylcytosines At The Specific DNA Sequence C(Me)Cgg. [C]

COG id: COG3183

COG function: function code V; Predicted restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.21.- [C]

Molecular weight: Translated: 14082; Mature: 14082

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIREGTLLAANHYRRERNPKLRKAKLAEFRRVHSRVFCEVCGFDFEAVYGERGAGYIEC
CCCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEE
HHIVPLHVSGETVTKLSDLVLLCSNCHRMIHHGSRWLSPEELREVVQVSGQPAEPGDGER
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
VEF
CCC
>Mature Secondary Structure
MEIREGTLLAANHYRRERNPKLRKAKLAEFRRVHSRVFCEVCGFDFEAVYGERGAGYIEC
CCCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEE
HHIVPLHVSGETVTKLSDLVLLCSNCHRMIHHGSRWLSPEELREVVQVSGQPAEPGDGER
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
VEF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA