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The map label for this gene is limA [H]

Identifier: 54026163

GI number: 54026163

Start: 4340784

End: 4341215

Strand: Reverse

Name: limA [H]

Synonym: nfa41920

Alternate gene names: 54026163

Gene position: 4341215-4340784 (Counterclockwise)

Preceding gene: 54026165

Following gene: 54026162

Centisome position: 72.1

GC content: 68.98

Gene sequence:

>432_bases
TTGTTTGCTTCAATCGGAAGCCGTCCCCGCCGCCGACGAGAAGAGTGCCCCATGACCGACCCCGACGCCGTCGTCCGCGC
CCTGTGCCAGGCCTGGTCGACCCCCGACCCCGACGCGATCGCGGCCTACTTCGCCGAGGACGCCGTGTACCACAACATCC
CGATGGATCCGGTCGTCGGCCGCACCGCCATCCGGGAGTTCGTCGCGGGGTTCCTGACCACCTTCGAGGCCATCGATTTC
GAGATCCACCACCAGGTGGCCGCCGACGGCGTGGTGATGAACGAGCGCACCGACACCATGCGCGGCAAGGACGGCCGAGC
CACGCCGCTGCCGGTCATGGGTGTGTTCGAGGTGCGCGACGGCCTGATCACGGCCTGGCGCGACTACTTCGACATGGCGG
CCGTGACCGCCGCGTTCGCGCCACCGGAGTGA

Upstream 100 bases:

>100_bases
CTGCGGCAACGCATTCTGACGTCGGGCGCGGCTGTGTAGCGTGCGAAGGCCCCGGCGGGCCTCGGCGCGCAATCCGCCGA
ACCCGCGTGAGACCAAGCGG

Downstream 100 bases:

>100_bases
GCCGCCGGGCGCCACGGGCGTGTGACGGGCGGATGAACGAAACTTCGCAACCTTGCCAGCCAAAGTTGTGAAGAAGATGC
CGAAGCGGGACTTCGGTGTC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MFASIGSRPRRRREECPMTDPDAVVRALCQAWSTPDPDAIAAYFAEDAVYHNIPMDPVVGRTAIREFVAGFLTTFEAIDF
EIHHQVAADGVVMNERTDTMRGKDGRATPLPVMGVFEVRDGLITAWRDYFDMAAVTAAFAPPE

Sequences:

>Translated_143_residues
MFASIGSRPRRRREECPMTDPDAVVRALCQAWSTPDPDAIAAYFAEDAVYHNIPMDPVVGRTAIREFVAGFLTTFEAIDF
EIHHQVAADGVVMNERTDTMRGKDGRATPLPVMGVFEVRDGLITAWRDYFDMAAVTAAFAPPE
>Mature_143_residues
MFASIGSRPRRRREECPMTDPDAVVRALCQAWSTPDPDAIAAYFAEDAVYHNIPMDPVVGRTAIREFVAGFLTTFEAIDF
EIHHQVAADGVVMNERTDTMRGKDGRATPLPVMGVFEVRDGLITAWRDYFDMAAVTAAFAPPE

Specific function: Catalyzes the conversion of limonene-1,2-epoxide to limonene-1,2-diol. Can use both the (-) and (+) isomers of limonene-1,2-epoxide as substrates and also has some activity with 1-methylcyclohexene oxide, cyclohexene oxide and indene oxide as substrates [

COG id: COG4308

COG function: function code Q; Limonene-1,2-epoxide hydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the limonene-1,2-epoxide hydrolase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013100 [H]

Pfam domain/function: PF07858 LEH [H]

EC number: =3.3.2.8 [H]

Molecular weight: Translated: 15865; Mature: 15865

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFASIGSRPRRRREECPMTDPDAVVRALCQAWSTPDPDAIAAYFAEDAVYHNIPMDPVVG
CCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
RTAIREFVAGFLTTFEAIDFEIHHQVAADGVVMNERTDTMRGKDGRATPLPVMGVFEVRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHCCCCCCCCCCCCHHHHHHHH
GLITAWRDYFDMAAVTAAFAPPE
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFASIGSRPRRRREECPMTDPDAVVRALCQAWSTPDPDAIAAYFAEDAVYHNIPMDPVVG
CCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
RTAIREFVAGFLTTFEAIDFEIHHQVAADGVVMNERTDTMRGKDGRATPLPVMGVFEVRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHCCCCCCCCCCCCHHHHHHHH
GLITAWRDYFDMAAVTAAFAPPE
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9827564; 9748436 [H]