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The map label for this gene is tsf

Identifier: 54026101

GI number: 54026101

Start: 4277952

End: 4278779

Strand: Reverse

Name: tsf

Synonym: nfa41300

Alternate gene names: 54026101

Gene position: 4278779-4277952 (Counterclockwise)

Preceding gene: 54026102

Following gene: 54026100

Centisome position: 71.06

GC content: 70.05

Gene sequence:

>828_bases
ATGGCGAACTACACCGCTGCCGATGTGAAGCGGCTCCGCGAGCTCACCGGCTCCGGAATGATGGACTGCAAGAACGCGCT
GGCCGAGACCGACGGCGACTTCGACAAGGCCGTCGAGCTGCTGCGCATCAAGGGCGCCAAGGACGTGGGCAAGCGCGCCG
AGCGCACCACCGCCGAGGGCCTGGTCGCCGCCAAGGACGGCGTGATGATCGAGATCAACTCCGAGACCGACTTCGTCGCC
AAGAACGACGAGTTCCAGGCGCTCGCGAACCAGATCGTCACCGCCGCCGCGGCCGCCAAGACCGCCGACCTGGACAGCCT
CAAGGCGCTGGACCTGGGCGACGGCCGTACCGCCGACGCCGCCCTGCAGGAGCTGGCCGCCAAGATCGGCGAGAAGCTCG
AGCTGCGCCGCGTGGTGTCGCTCGACGGCCCGGTCGCCACCTACCTGCACAAGCGTGCCTCCGACCTGCCGCCCGCCGTC
GGCGTGCTGGTGGAGTACCAGGGTGCGGGCGACGCCGCGGCCGAGGCCGCCCGTGCCGCCGCCATGCAGGTCGCCGCGCT
CAAGGCCAAGTACGTGACCCGCGACGAGGTCCCCGCCGACATCGTGGAGAACGAGCGCCGCATCGCCGAGCAGACCGCTC
GCGAGGAGGGCAAGCCCGAGGCCGCCCTGCCCAAGATCACCGAGGGCCGCGTCAACGGCTTCTTCAAGGACGTCGTCCTG
CTGGAGCAGCCGTCGGTGACCGACAACAAGAAGACCGTCAAGCAGCAGCTGGACGAAGCCGGTGTCACCGTCACCCGCTT
CGCCCGCTTCGAGGTCGGCCAGGCCTGA

Upstream 100 bases:

>100_bases
TCGGAGCCGTTCGTTCACCTTGCCGACCCTTCTGCGTTCAGGAGCGGTCGGCAAGGTGGTTTTCCCACCCCGACCTAGAC
ACTGAGGAGGCTCGCCACCG

Downstream 100 bases:

>100_bases
CCCCGAGCTCAGGCAGGCCCCCCGCGTGACAACGGCGTCGTGCGGGGGGCCTGTTGCTTTGTCCGGGTGCCGAATCACGC
GATCGATATTCCGGGGCGCG

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MANYTAADVKRLRELTGSGMMDCKNALAETDGDFDKAVELLRIKGAKDVGKRAERTTAEGLVAAKDGVMIEINSETDFVA
KNDEFQALANQIVTAAAAAKTADLDSLKALDLGDGRTADAALQELAAKIGEKLELRRVVSLDGPVATYLHKRASDLPPAV
GVLVEYQGAGDAAAEAARAAAMQVAALKAKYVTRDEVPADIVENERRIAEQTAREEGKPEAALPKITEGRVNGFFKDVVL
LEQPSVTDNKKTVKQQLDEAGVTVTRFARFEVGQA

Sequences:

>Translated_275_residues
MANYTAADVKRLRELTGSGMMDCKNALAETDGDFDKAVELLRIKGAKDVGKRAERTTAEGLVAAKDGVMIEINSETDFVA
KNDEFQALANQIVTAAAAAKTADLDSLKALDLGDGRTADAALQELAAKIGEKLELRRVVSLDGPVATYLHKRASDLPPAV
GVLVEYQGAGDAAAEAARAAAMQVAALKAKYVTRDEVPADIVENERRIAEQTAREEGKPEAALPKITEGRVNGFFKDVVL
LEQPSVTDNKKTVKQQLDEAGVTVTRFARFEVGQA
>Mature_274_residues
ANYTAADVKRLRELTGSGMMDCKNALAETDGDFDKAVELLRIKGAKDVGKRAERTTAEGLVAAKDGVMIEINSETDFVAK
NDEFQALANQIVTAAAAAKTADLDSLKALDLGDGRTADAALQELAAKIGEKLELRRVVSLDGPVATYLHKRASDLPPAVG
VLVEYQGAGDAAAEAARAAAMQVAALKAKYVTRDEVPADIVENERRIAEQTAREEGKPEAALPKITEGRVNGFFKDVVLL
EQPSVTDNKKTVKQQLDEAGVTVTRFARFEVGQA

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Escherichia coli, GI1786366, Length=279, Percent_Identity=41.2186379928315, Blast_Score=181, Evalue=6e-47,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_NOCFA (Q5YS62)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120343.1
- ProteinModelPortal:   Q5YS62
- SMR:   Q5YS62
- GeneID:   3111616
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa41300
- NMPDR:   fig|247156.1.peg.3987
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   NFAR247156:NFA41300-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 29356; Mature: 29225

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANYTAADVKRLRELTGSGMMDCKNALAETDGDFDKAVELLRIKGAKDVGKRAERTTAEG
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHC
LVAAKDGVMIEINSETDFVAKNDEFQALANQIVTAAAAAKTADLDSLKALDLGDGRTADA
EEEECCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCCHHH
ALQELAAKIGEKLELRRVVSLDGPVATYLHKRASDLPPAVGVLVEYQGAGDAAAEAARAA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHEEEEEECCCCHHHHHHHHHH
AMQVAALKAKYVTRDEVPADIVENERRIAEQTAREEGKPEAALPKITEGRVNGFFKDVVL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHE
LEQPSVTDNKKTVKQQLDEAGVTVTRFARFEVGQA
ECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure 
ANYTAADVKRLRELTGSGMMDCKNALAETDGDFDKAVELLRIKGAKDVGKRAERTTAEG
CCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHC
LVAAKDGVMIEINSETDFVAKNDEFQALANQIVTAAAAAKTADLDSLKALDLGDGRTADA
EEEECCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCCHHH
ALQELAAKIGEKLELRRVVSLDGPVATYLHKRASDLPPAVGVLVEYQGAGDAAAEAARAA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHEEEEEECCCCHHHHHHHHHH
AMQVAALKAKYVTRDEVPADIVENERRIAEQTAREEGKPEAALPKITEGRVNGFFKDVVL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHE
LEQPSVTDNKKTVKQQLDEAGVTVTRFARFEVGQA
ECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA