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The map label for this gene is rlmN

Identifier: 54026096

GI number: 54026096

Start: 4273793

End: 4274902

Strand: Reverse

Name: rlmN

Synonym: nfa41250

Alternate gene names: 54026096

Gene position: 4274902-4273793 (Counterclockwise)

Preceding gene: 54026097

Following gene: 54026093

Centisome position: 71.0

GC content: 72.16

Gene sequence:

>1110_bases
ATGACGGTCTCCCTCCCCCTGGTTTTCGACGCTCCGCGCCGTGGCATGCCGCCCCGGCACCTCGCCGACCTCGATGCCGA
GCAGCGGCGCGCGGCGGTGGCCGAGCTGGGGCTGCCGAAATTCCGGGCCGACCAGATCGCCCGCCAGTACTACGGGCGGC
TGGAGGCCGATCCGGCGCGGATGACCGATCTGCCTGCCCCGGTCCGCGAGCAGATCGGTGCGGCGCTGTTCCCGCGCCTG
CTGGACGTGGTCAAGCACGTGGCCTGCGACGACGGCCGCACCCGCAAGACGCTGTGGCGGGCCGGTGACGGCACGTTGCT
GGAGAGCGTGCTCATGCGCTACGCCGACCGCAACACCCTGTGCATCTCCAGCCAGGCCGGCTGCGGCATGGCCTGCCCCT
TCTGTGCCACCGGTCAGGGTGGGCTGAACCGCAACCTGTCCACCGCCGAGATCGTCGACCAGGTCCGCGCGGCCGCGGCC
GCCCTGCGCGACGGTGCGGTCGCGGGCGGGCCGGGCCGGCTGTCCAACATCGTGTTCATGGGCATGGGCGAGCCGCTGGC
CAACTACAAGCGCGTGGTCGCCGCGGTGCGCCGCATCACCGCACCCGCTCCCGACGGGCTCGGCATCTCCCAGCGCAACG
TCGTCGTCTCCACCGTCGGCTTGGCCCCCGCCATCCGCAAGCTGGCCGACGAGGGGCTCTCGGTCACCCTCGCGGTCTCC
CTGCACACCCCCGACGACGAGCTGCGCGACACCCTGGTGCCGGTGAACAACCGCTGGCCCGTCGCCGAGGTGCTCGACGC
CGCCCGCTACTACGCCGACACGACCGGCCGCCGGGTGTCGGTCGAGTACGCGCTCATCCGCGACATCAACGATCAGCCCT
GGCGCGCCGACATGCTCGGTGCCAAGCTGCACAAGGCGCTGGGCTCGCGGGTGCACGTCAACGTGATCCCGCTCAACCCG
ACTCCGGGCAGCAAGTGGGATGCCAGCCCGAAGCCGGTCGAGCGGGAATTCGTGCGCCGTGTCAACGAGCAGGGCGTGCC
GTGCACGGTGCGCGACACGCGCGGCCAGGAGATCGCCGCGGCGTGCGGACAGCTCGCCGCCGAGAACTGA

Upstream 100 bases:

>100_bases
TGGTCGGCGGCGTGCGGATTCTCCAGTTGCGGCGCGCCGCCAAGCACTGATTCCGGGCGGCTGCACGGCACCCGGGCGGA
GATGGGACACTGGAAGCACT

Downstream 100 bases:

>100_bases
CCCGACGCGCCACCGCCGGGTCAGGGCAGCCGCCGCGGGGTCAGGGAATCAGCACGGTTCGACCCACCGCGGTCCTGGTC
TCGATCGCGGCGTGCGCGCC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTVSLPLVFDAPRRGMPPRHLADLDAEQRRAAVAELGLPKFRADQIARQYYGRLEADPARMTDLPAPVREQIGAALFPRL
LDVVKHVACDDGRTRKTLWRAGDGTLLESVLMRYADRNTLCISSQAGCGMACPFCATGQGGLNRNLSTAEIVDQVRAAAA
ALRDGAVAGGPGRLSNIVFMGMGEPLANYKRVVAAVRRITAPAPDGLGISQRNVVVSTVGLAPAIRKLADEGLSVTLAVS
LHTPDDELRDTLVPVNNRWPVAEVLDAARYYADTTGRRVSVEYALIRDINDQPWRADMLGAKLHKALGSRVHVNVIPLNP
TPGSKWDASPKPVEREFVRRVNEQGVPCTVRDTRGQEIAAACGQLAAEN

Sequences:

>Translated_369_residues
MTVSLPLVFDAPRRGMPPRHLADLDAEQRRAAVAELGLPKFRADQIARQYYGRLEADPARMTDLPAPVREQIGAALFPRL
LDVVKHVACDDGRTRKTLWRAGDGTLLESVLMRYADRNTLCISSQAGCGMACPFCATGQGGLNRNLSTAEIVDQVRAAAA
ALRDGAVAGGPGRLSNIVFMGMGEPLANYKRVVAAVRRITAPAPDGLGISQRNVVVSTVGLAPAIRKLADEGLSVTLAVS
LHTPDDELRDTLVPVNNRWPVAEVLDAARYYADTTGRRVSVEYALIRDINDQPWRADMLGAKLHKALGSRVHVNVIPLNP
TPGSKWDASPKPVEREFVRRVNEQGVPCTVRDTRGQEIAAACGQLAAEN
>Mature_368_residues
TVSLPLVFDAPRRGMPPRHLADLDAEQRRAAVAELGLPKFRADQIARQYYGRLEADPARMTDLPAPVREQIGAALFPRLL
DVVKHVACDDGRTRKTLWRAGDGTLLESVLMRYADRNTLCISSQAGCGMACPFCATGQGGLNRNLSTAEIVDQVRAAAAA
LRDGAVAGGPGRLSNIVFMGMGEPLANYKRVVAAVRRITAPAPDGLGISQRNVVVSTVGLAPAIRKLADEGLSVTLAVSL
HTPDDELRDTLVPVNNRWPVAEVLDAARYYADTTGRRVSVEYALIRDINDQPWRADMLGAKLHKALGSRVHVNVIPLNPT
PGSKWDASPKPVEREFVRRVNEQGVPCTVRDTRGQEIAAACGQLAAEN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=352, Percent_Identity=37.5, Blast_Score=212, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_NOCFA (Q5YS67)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120338.1
- ProteinModelPortal:   Q5YS67
- SMR:   Q5YS67
- GeneID:   3108910
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa41250
- NMPDR:   fig|247156.1.peg.3982
- HOGENOM:   HBG335345
- OMA:   DINDQPW
- ProtClustDB:   PRK14459
- BioCyc:   NFAR247156:NFA41250-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39875; Mature: 39744

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSLPLVFDAPRRGMPPRHLADLDAEQRRAAVAELGLPKFRADQIARQYYGRLEADPAR
CEEEEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHH
MTDLPAPVREQIGAALFPRLLDVVKHVACDDGRTRKTLWRAGDGTLLESVLMRYADRNTL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEE
CISSQAGCGMACPFCATGQGGLNRNLSTAEIVDQVRAAAAALRDGAVAGGPGRLSNIVFM
EEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCEEEE
GMGEPLANYKRVVAAVRRITAPAPDGLGISQRNVVVSTVGLAPAIRKLADEGLSVTLAVS
ECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCEEEEEEE
LHTPDDELRDTLVPVNNRWPVAEVLDAARYYADTTGRRVSVEYALIRDINDQPWRADMLG
EECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHHHHH
AKLHKALGSRVHVNVIPLNPTPGSKWDASPKPVEREFVRRVNEQGVPCTVRDTRGQEIAA
HHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHH
ACGQLAAEN
HHHHHHCCC
>Mature Secondary Structure 
TVSLPLVFDAPRRGMPPRHLADLDAEQRRAAVAELGLPKFRADQIARQYYGRLEADPAR
EEEEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHH
MTDLPAPVREQIGAALFPRLLDVVKHVACDDGRTRKTLWRAGDGTLLESVLMRYADRNTL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEE
CISSQAGCGMACPFCATGQGGLNRNLSTAEIVDQVRAAAAALRDGAVAGGPGRLSNIVFM
EEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCEEEE
GMGEPLANYKRVVAAVRRITAPAPDGLGISQRNVVVSTVGLAPAIRKLADEGLSVTLAVS
ECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCEEEEEEE
LHTPDDELRDTLVPVNNRWPVAEVLDAARYYADTTGRRVSVEYALIRDINDQPWRADMLG
EECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHHHHH
AKLHKALGSRVHVNVIPLNPTPGSKWDASPKPVEREFVRRVNEQGVPCTVRDTRGQEIAA
HHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHH
ACGQLAAEN
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA